PIAS1

PIAS1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1V66
Identifiers
Aliases	PIAS1 , DDXBP1, GBP, GU/RH-II, ZMIZ3, protein inhibitor of activated STAT 1
External IDs	OMIM: 603566; MGI: 1913125; HomoloGene: 22953; GeneCards: PIAS1; OMA:PIAS1 - orthologs
Gene location (Human) Chr. Chromosome 15 (human) [1] Band 15q23 Start 68,054,309 bp [1] End 68,198,603 bp [1]
Gene location (Mouse) Chr. Chromosome 9 (mouse) [2] Band 9|9 B Start 62,785,650 bp [2] End 62,895,206 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte seminal vesicula nipple caput epididymis tail of epididymis bone marrow cells cardia trabecular bone Achilles tendon buccal mucosa cell Top expressed in zygote spermatid secondary oocyte primary oocyte seminiferous tubule pineal gland genital tubercle spermatocyte olfactory tubercle granulocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding transcription corepressor activity transcription coactivator activity zinc ion binding transcription factor binding metal ion binding SUMO ligase activity protein binding androgen receptor binding enzyme binding ubiquitin protein ligase binding protein domain specific binding SUMO transferase activity transferase activity protein C-terminus binding Cellular component PML body nuclear speck nucleoplasm nucleus Biological process androgen receptor signaling pathway receptor signaling pathway via JAK-STAT regulation of transcription, DNA-templated negative regulation of transcription by RNA polymerase II protein sumoylation transcription, DNA-templated regulation of interferon-gamma-mediated signaling pathway regulation of cell population proliferation fat cell differentiation positive regulation of proteasomal ubiquitin-dependent protein catabolic process visual learning negative regulation of apoptotic process positive regulation of smooth muscle cell differentiation spermatogenesis positive regulation of protein sumoylation protein-DNA complex assembly positive regulation of transcription, DNA-templated G1/S transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8554 56469 Ensembl ENSG00000033800 ENSMUSG00000032405 UniProt O75925 O88907 RefSeq (mRNA) NM_016166 NM_001320687 NM_019663 RefSeq (protein) NP_001307616 NP_057250 NP_062637 Location (UCSC) Chr 15: 68.05 – 68.2 Mb Chr 9: 62.79 – 62.9 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. ^{[5] [6] [7]}

Function

This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. ^[7]

Interactions

PIAS1 has been shown to interact with:

• DNMT3A, ^[8]
• P53, ^{[9] [10]}
• STAT1, ^[11]
• Small ubiquitin-related modifier 1, ^{[9] [12]}
• Sp3 transcription factor, ^[13] and
• UBE2I. ^{[9] [12] [13]}

References

1. GRCh38: Ensembl release 89: ENSG00000033800 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032405 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Liu B, Liao J, Rao X, Kushner SA, Chung CD, Chang DD, Shuai K (September 1998). "Inhibition of Stat1-mediated gene activation by PIAS1". Proc Natl Acad Sci U S A. 95 (18): 10626–10631. Bibcode:1998PNAS...9510626L. doi: 10.1073/pnas.95.18.10626 . PMC   27945 . PMID   9724754.
6. Valdez BC, Henning D, Perlaky L, Busch RK, Busch H (July 1997). "Cloning and characterization of Gu/RH-II binding protein". Biochem Biophys Res Commun. 234 (2): 335–340. doi:10.1006/bbrc.1997.6642. PMID   9177271.
7. "Entrez Gene: PIAS1 protein inhibitor of activated STAT, 1".
8. Ling Y, Sankpal UT, Robertson AK, McNally JG, Karpova T, Robertson KD (2004). "Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription". Nucleic Acids Res. 32 (2): 598–610. doi:10.1093/nar/gkh195. PMC   373322 . PMID   14752048.
9. Kahyo T, Nishida T, Yasuda H (September 2001). "Involvement of PIAS1 in the sumoylation of tumor suppressor p53". Mol. Cell. 8 (3): 713–8. doi: 10.1016/s1097-2765(01)00349-5 . PMID   11583632.
10. Gallagher WM, Argentini M, Sierra V, Bracco L, Debussche L, Conseiller E (June 1999). "MBP1: a novel mutant p53-specific protein partner with oncogenic properties". Oncogene. 18 (24): 3608–16. doi: 10.1038/sj.onc.1202937 . PMID   10380882.
11. Liao J, Fu Y, Shuai K (May 2000). "Distinct roles of the NH2- and COOH-terminal domains of the protein inhibitor of activated signal transducer and activator of transcription (STAT) 1 (PIAS1) in cytokine-induced PIAS1-Stat1 interaction". Proc. Natl. Acad. Sci. U.S.A. 97 (10): 5267–72. Bibcode:2000PNAS...97.5267L. doi: 10.1073/pnas.97.10.5267 . PMC   25817 . PMID   10805787.
12. Lee BH, Yoshimatsu K, Maeda A, Ochiai K, Morimatsu M, Araki K, Ogino M, Morikawa S, Arikawa J (December 2003). "Association of the nucleocapsid protein of the Seoul and Hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules". Virus Res. 98 (1): 83–91. doi:10.1016/j.virusres.2003.09.001. PMID   14609633.
13. Sapetschnig A, Rischitor G, Braun H, Doll A, Schergaut M, Melchior F, Suske G (October 2002). "Transcription factor Sp3 is silenced through SUMO modification by PIAS1". EMBO J. 21 (19): 5206–15. doi:10.1093/emboj/cdf510. PMC   129032 . PMID   12356736.

Further reading

• Tan J, Hall SH, Hamil KG, Grossman G, Petrusz P, Liao J, Shuai K, French FS (2000). "Protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis". Mol. Endocrinol. 14 (1): 14–26. doi: 10.1210/mend.14.1.0408 . PMID   10628744.
• Liao J, Fu Y, Shuai K (2000). "Distinct roles of the NH2- and COOH-terminal domains of the protein inhibitor of activated signal transducer and activator of transcription (STAT) 1 (PIAS1) in cytokine-induced PIAS1-Stat1 interaction". Proc. Natl. Acad. Sci. U.S.A. 97 (10): 5267–5272. Bibcode:2000PNAS...97.5267L. doi: 10.1073/pnas.97.10.5267 . PMC   25817 . PMID   10805787.
• Kotaja N, Aittomäki S, Silvennoinen O, Palvimo JJ, Jänne OA (2001). "ARIP3 (androgen receptor-interacting protein 3) and other PIAS (protein inhibitor of activated STAT) proteins differ in their ability to modulate steroid receptor-dependent transcriptional activation". Mol. Endocrinol. 14 (12): 1986–2000. doi: 10.1210/mend.14.12.0569 . PMID   11117529.
• Gross M, Liu B, Tan J, French FS, Carey M, Shuai K (2001). "Distinct effects of PIAS proteins on androgen-mediated gene activation in prostate cancer cells". Oncogene. 20 (29): 3880–3887. doi: 10.1038/sj.onc.1204489 . PMID   11439351.
• Liu B, Shuai K (2001). "Induction of apoptosis by protein inhibitor of activated Stat1 through c-Jun NH2-terminal kinase activation". J. Biol. Chem. 276 (39): 36624–36631. doi: 10.1074/jbc.M101085200 . PMID   11451946.
• Kahyo T, Nishida T, Yasuda H (2001). "Involvement of PIAS1 in the sumoylation of tumor suppressor p53". Mol. Cell. 8 (3): 713–718. doi: 10.1016/S1097-2765(01)00349-5 . PMID   11583632.
• Weiskirchen R, Moser M, Weiskirchen S, Erdel M, Dahmen S, Buettner R, Gressner AM (2001). "LIM-domain protein cysteine- and glycine-rich protein 2 (CRP2) is a novel marker of hepatic stellate cells and binding partner of the protein inhibitor of activated STAT1". Biochem. J. 359 (Pt 3): 485–96. doi:10.1042/0264-6021:3590485. PMC   1222169 . PMID   11672422.
• Megidish T, Xu JH, Xu CW (2002). "Activation of p53 by protein inhibitor of activated Stat1 (PIAS1)". J. Biol. Chem. 277 (10): 8255–8259. doi: 10.1074/jbc.C200001200 . PMID   11788578.
• Schmidt D, Müller S (2002). "Members of the PIAS family act as SUMO ligases for c-Jun and p53 and repress p53 activity". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2872–2877. Bibcode:2002PNAS...99.2872S. doi: 10.1073/pnas.052559499 . PMC   122440 . PMID   11867732.
• Tan JA, Hall SH, Hamil KG, Grossman G, Petrusz P, French FS (2002). "Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators". J. Biol. Chem. 277 (19): 16993–17001. doi: 10.1074/jbc.M109217200 . PMID   11877418.
• Nishida T, Yasuda H (2003). "PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor and repress androgen receptor-dependent transcription". J. Biol. Chem. 277 (44): 41311–41317. doi: 10.1074/jbc.M206741200 . PMID   12177000.
• Rui HL, Fan E, Zhou HM, Xu Z, Zhang Y, Lin SC (2003). "SUMO-1 modification of the C-terminal KVEKVD of Axin is required for JNK activation but has no effect on Wnt signaling". J. Biol. Chem. 277 (45): 42981–42986. doi: 10.1074/jbc.M208099200 . PMID   12223491.
• Sapetschnig A, Rischitor G, Braun H, Doll A, Schergaut M, Melchior F, Suske G (2002). "Transcription factor Sp3 is silenced through SUMO modification by PIAS1". EMBO J. 21 (19): 5206–5215. doi:10.1093/emboj/cdf510. PMC   129032 . PMID   12356736.
• Miyauchi Y, Yogosawa S, Honda R, Nishida T, Yasuda H (2003). "Sumoylation of Mdm2 by protein inhibitor of activated STAT (PIAS) and RanBP2 enzymes". J. Biol. Chem. 277 (51): 50131–50136. doi: 10.1074/jbc.M208319200 . PMID   12393906.
• Ungureanu D, Vanhatupa S, Kotaja N, Yang J, Aittomaki S, Jänne OA, Palvimo JJ, Silvennoinen O (2004). "PIAS proteins promote SUMO-1 conjugation to STAT1". Blood. 102 (9): 3311–3313. doi: 10.1182/blood-2002-12-3816 . PMID   12855578.
• Kadaré G, Toutant M, Formstecher E, Corvol JC, Carnaud M, Boutterin MC, Girault JA (2004). "PIAS1-mediated sumoylation of focal adhesion kinase activates its autophosphorylation". J. Biol. Chem. 278 (48): 47434–47440. doi: 10.1074/jbc.M308562200 . PMID   14500712.
• Tallec LP, Kirsh O, Lecomte MC, Viengchareun S, Zennaro MC, Dejean A, Lombès M (2004). "Protein inhibitor of activated signal transducer and activator of transcription 1 interacts with the N-terminal domain of mineralocorticoid receptor and represses its transcriptional activity: implication of small ubiquitin-related modifier 1 modification". Mol. Endocrinol. 17 (12): 2529–2542. doi: 10.1210/me.2003-0299 . PMID   14500761.
• Coppola D, Parikh V, Boulware D, Blanck G (2009). "Substantially reduced expression of PIAS1 is associated with colon cancer development". J Cancer Res Clin Oncol. 135 (9): 1287–91. doi:10.1007/s00432-009-0570-z. PMID   19288270. S2CID   20383429.