Pheophorbide a oxygenase

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Pheophorbide a oxygenase
Identifiers
EC no. 1.14.15.17
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Pheophorbide a oxygenase (EC 1.14.15.17, pheide a monooxygenase, pheide a oxygenase, PAO) is an enzyme with systematic name pheophorbide-a,NADPH:oxygen oxidoreductase (biladiene-forming). [1] [2] [3] [4] [5] [6] This enzyme catalyses the following chemical reaction

Pheophorbide a + NADPH + H+ + O2 red chlorophyll catabolite + NADP+

Pheophorbide a oxygenase participates in chlorophyll degradation. Loss-of-function mutations in the gene can lead to a stay-green phenotype in plants. [7]

References

  1. Hörtensteiner S, Wüthrich KL, Matile P, Ongania KH, Kräutler B (June 1998). "The key step in chlorophyll breakdown in higher plants. Cleavage of pheophorbide a macrocycle by a monooxygenase". The Journal of Biological Chemistry. 273 (25): 15335–9. doi: 10.1074/jbc.273.25.15335 . PMID   9624113.
  2. Pruzinská A, Tanner G, Anders I, Roca M, Hörtensteiner S (December 2003). "Chlorophyll breakdown: pheophorbide a oxygenase is a Rieske-type iron-sulfur protein, encoded by the accelerated cell death 1 gene". Proceedings of the National Academy of Sciences of the United States of America. 100 (25): 15259–64. Bibcode:2003PNAS..10015259P. doi: 10.1073/pnas.2036571100 . PMC   299977 . PMID   14657372.
  3. Chung DW, Pruzinská A, Hörtensteiner S, Ort DR (September 2006). "The role of pheophorbide a oxygenase expression and activity in the canola green seed problem". Plant Physiology. 142 (1): 88–97. doi:10.1104/pp.106.084483. PMC   1557622 . PMID   16844830.
  4. Rodoni S, Muhlecker W, Anderl M, Krautler B, Moser D, Thomas H, Matile P, Hortensteiner S (October 1997). "Chlorophyll Breakdown in Senescent Chloroplasts (Cleavage of Pheophorbide a in Two Enzymic Steps)". Plant Physiology. 115 (2): 669–676. doi:10.1104/pp.115.2.669. PMC   158527 . PMID   12223835.
  5. Hörtensteiner S (2006). "Chlorophyll degradation during senescence". Annual Review of Plant Biology. 57 (1): 55–77. Bibcode:2006AnRPB..57...55H. doi:10.1146/annurev.arplant.57.032905.105212. PMID   16669755.
  6. Pruzinská A, Anders I, Aubry S, Schenk N, Tapernoux-Lüthi E, Müller T, Kräutler B, Hörtensteiner S (January 2007). "In vivo participation of red chlorophyll catabolite reductase in chlorophyll breakdown". The Plant Cell. 19 (1): 369–87. Bibcode:2007PlanC..19..369P. doi:10.1105/tpc.106.044404. PMC   1820978 . PMID   17237353.
  7. Bachmann, Andre; Fernandez-Lopez, Jose; Ginsburg, Samuel; Thomas, Howard; Bouwcamp, John C; Solomos, Theophanes; Matile, Phillipe (1994). "Stay-green genotypes of Phaseolus vulgaris L.: chloroplast proteins and chlorophyll catabolites during foliar senescence". New Phytologist. 126 (4): 593–600. Bibcode:1994NewPh.126..593B. doi: 10.1111/j.1469-8137.1994.tb02953.x .