Glycosylphosphatidylinositol diacylglycerol-lyase

glycosylphosphatidylinositol diacylglycerol-lyase
Identifiers
EC no.	4.6.1.14
CAS no.	129070-68-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The enzyme glycosylphosphatidylinositol diacylglycerol-lyase (EC 4.6.1.14) catalyzes the reaction

6-(α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol = 6-(α-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

This enzyme belongs to the family of lyases, specifically the class of phosphorus-oxygen lyases. The systematic name of this enzyme class is 6-(α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol 1,2-diacyl-sn-glycerol-lyase [6-(α-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate-forming].

Other names in common use include:

• GPI-PLC
• GPI-specific phospholipase C
• Glycosyl inositol phospholipid anchor-hydrolyzing enzyme
• Glycosylphosphatidylinositol-phospholipase C
• Glycosylphosphatidylinositol-specific phospholipase C or VSG-lipase ^[1]
• Variant-surface-glycoprotein phospholipase C
• 6-(α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
• Diacylglycerol-lyase (1,2-cyclic-phosphate-forming)

References

1. Paturiaux-Hanocq, F.; Hanocq-Quertier, J.; de Almeida, M. L.; Nolan, D. P.; Pays, A.; Vanhamme, L.; Van den Abbeele, J.; Wasunna, C. L.; Carrington, M.; Pays, E. (2000-04-21). "A role for the dynamic acylation of a cluster of cysteine residues in regulating the activity of the glycosylphosphatidylinositol-specific phospholipase C of Trypanosoma brucei". The Journal of Biological Chemistry. 275 (16): 12147–12155. doi: 10.1074/jbc.275.16.12147 . ISSN   0021-9258. PMID   10766850.

• Hereld D, Krakow JL, Bangs JD, Hart GW, Englund PT (1986). "A phospholipase C from Trypanosoma brucei which selectively cleaves the glycolipid on the variant surface glycoprotein". J. Biol. Chem. 261 (29): 13813–9. doi: 10.1016/S0021-9258(18)67092-9 . PMID   3759991.
• Carnall N, Webb H, Carrington M (1997). "Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei". Mol. Biochem. Parasitol. 90 (2): 423–32. doi:10.1016/S0166-6851(97)00177-1. PMID   9476790.
• Armah DA, Mensa-Wilmot K (2000). "Tetramerization of glycosylphosphatidylinositol-specific phospholipase C from Trypanosoma brucei". J. Biol. Chem. 275 (25): 19334–42. doi: 10.1074/jbc.M001798200 . PMID   10764777.