Phosphatidylinositol diacylglycerol-lyase

phosphatidylinositol diacylglycerol-lyase
Identifiers
EC no.	4.6.1.13
CAS no.	37288-19-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Phosphatidylinositol-specific phospholipase C, image from OPM database

The enzyme phosphatidylinositol diacylglycerol-lyase (EC 4.6.1.13) catalyzes the following reaction:

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol

This enzyme belongs to the family of lyases, specifically the class of phosphorus-oxygen lyases. The systematic name of this enzyme class is 1-phosphatidyl-1D-myo-inositol 1,2-diacyl-sn-glycerol-lyase (1D-myo-inositol-1,2-cyclic-phosphate-forming). Other names in common use include monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, 1-phosphatidylinositol phosphodiesterase, 1-phosphatidyl-D-myo-inositol inositolphosphohydrolase, (cyclic-phosphate-forming), 1-phosphatidyl-1D-myo-inositol diacylglycerol-lyase, and (1,2-cyclic-phosphate-forming). It participates in inositol phosphate metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1T6M and 2OR2.

References

• Allan D, Michell RH (1974). "Phosphatidylinositol cleavage catalysed by the soluble fraction from lymphocytes. Activity at pH5.5 and pH7.0". Biochem. J. 142 (3): 591–7. doi:10.1042/bj1420591. PMC   1168323 . PMID   4377210.
• Friedel RO, Brown JD, Durell (1967). "Monophosphatidyl inositol inositolphosphohydrolase in guinea-pig brain". Biochim. Biophys. Acta. 144 (3): 684–6. doi:10.1016/0005-2760(67)90059-8. PMID   4294905.
• Irvine RF (1982). "The enzymology of stimulated inositol lipid turnover". Cell Calcium. 3 (4–5): 295–309. doi:10.1016/0143-4160(82)90018-5. PMID   6297738.
• Michell RH, Allan D (1975). "Inositol cyclis phosphate as a product of phosphatidylinositol breakdown by phospholipase C (Bacillus cereus)". FEBS Lett. 53 (3): 302–4. Bibcode:1975FEBSL..53..302M. doi: 10.1016/0014-5793(75)80041-X . PMID   236918. S2CID   5813981.
• Low MG, Finean JB (1977). "Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C". Biochem. J. 167 (1): 281–4. doi:10.1042/bj1670281. PMC   1183647 . PMID   588258.
• Henner DJ, Yang M, Chen E, Hellmiss R, Rodriguez H, Low MG (1988). "Sequence of the Bacillus thuringiensis phosphatidylinositol specific phospholipase C". Nucleic Acids Res. 16 (21) 10383. doi:10.1093/nar/16.21.10383. PMC   338883 . PMID   3194218.