RPMI 1640

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RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [2] A modification of McCoy′s 5A medium (or RPMI 1630), it was originally formulated to support lymphoblastoid cells in suspension cultures, but can also support a wide variety of adherent cells.

Contents

It was originally developed to culture human leukemic cells. Over the years, the original formulation was modified and refined by researchers and commercial suppliers to enhance its ability to support the growth of many cell types. This medium contains a great deal of phosphate, amino acids and vitamins. RPMI 1640 uses a bicarbonate buffering system and requires a 5–10% CO2 atmosphere to maintain physiological pH. Normally, the medium contains no proteins or growth factors, so it is commonly supplemented with 10% fetal bovine serum. Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human lymphocytes, Jurkat cells, HeLa cells, bone marrow cells, hybridomas and carcinomas.

Composition

Many different formulations exist. Typically, one liter of RPMI 1640 contains: [2]

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References

  1. Atlas RM, Snyder JW (2006). Handbook of Media for Clinical Microbiology (2nd ed.). Boca Raton, Florida: CRC Press. p. 427.
  2. 1 2 Moore GE, Gerner RE, Franklin HA (1967). "Culture of normal human leukocytes". JAMA. 199 (8): 519–524. doi:10.1001/jama.199.8.519. PMID   4960081.