CKAP5

CKAP5
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4QMI, 4QMJ
Identifiers
Aliases	CKAP5 , CHTOG, MSPS, TOG, TOGp, ch-TOG, cytoskeleton associated protein 5
External IDs	OMIM: 611142; MGI: 1923036; HomoloGene: 8844; GeneCards: CKAP5; OMA:CKAP5 - orthologs
Gene location (Human) Chr. Chromosome 11 (human) [1] Band 11p11.2 Start 46,743,048 bp [1] End 46,846,308 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 E1 Start 91,357,107 bp [2] End 91,451,009 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ventricular zone ganglionic eminence oocyte right testis stromal cell of endometrium Achilles tendon left testis secondary oocyte prefrontal cortex cerebellar hemisphere Top expressed in tail of embryo genital tubercle ventricular zone gastrula ganglionic eminence cerebellar cortex maxillary prominence secondary oocyte mandibular prominence zygote More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding cadherin binding microtubule plus-end binding Cellular component cytoplasm cytosol centrosome spindle pole membrane microtubule cytoskeleton gamma-tubulin complex microtubule organizing center microtubule plus-end cytoskeleton chromosome, centromeric region kinetochore chromosome spindle nucleolus plasma membrane protein-containing complex Biological process RNA transport establishment or maintenance of microtubule cytoskeleton polarity cell division spindle organization G2/M transition of mitotic cell cycle cell cycle sister chromatid cohesion centrosome cycle microtubule polymerization centrosome duplication ciliary basal body-plasma membrane docking regulation of G2/M transition of mitotic cell cycle microtubule depolymerization Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9793 75786 Ensembl ENSG00000175216 ENSMUSG00000040549 UniProt Q14008 A2AGT5 RefSeq (mRNA) NM_014756 NM_001008938 NM_001165989 NM_029437 RefSeq (protein) NP_001008938 NP_055571 NP_001159461 NP_083713 Location (UCSC) Chr 11: 46.74 – 46.85 Mb Chr 2: 91.36 – 91.45 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene. ^{[5] [6] [7]} It is the homolog of the Xenopus protein XMAP215 ^[8] and is also known as ch-Tog.

It has at least two distinct roles in spindle formation: it protects kinetochore microtubules from depolymerization by MCAK (KIF2C), and ch-Tog plays an essential role in centrosomal microtubule assembly, a function independent of MCAK activity. ^[9]

Interactions

CKAP5 has been shown to interact with TACC1. ^{[10] [11]}

References

1. GRCh38: Ensembl release 89: ENSG00000175216 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000040549 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Miyajima N, Tanaka A, Sazuka T, Seki N, Sato S, Tabata S, Ishikawa K, Kawarabayasi Y, Kotani H (Jul 1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi: 10.1093/dnares/2.1.37 . PMID   7788527.
6. Charrasse S, Mazel M, Taviaux S, Berta P, Chow T, Larroque C (Feb 1996). "Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors". Eur. J. Biochem. 234 (2): 406–13. doi: 10.1111/j.1432-1033.1995.406_b.x . PMID   8536682.
7. "Entrez Gene: CKAP5 cytoskeleton associated protein 5".
8. Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly". Mol. Biol. Cell. 15 (4): 1580–90. doi:10.1091/mbc.E03-07-0544. PMC   379257 . PMID   14718566.
9. Barr AR, Gergely F (2008). "MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics". Mol. Cell. Biol. 28 (23): 7199–211. doi:10.1128/MCB.01040-08. PMC   2593372 . PMID   18809577.
10. Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D (Nov 2003). "TACC1-chTOG-Aurora A protein complex in breast cancer". Oncogene. 22 (50): 8102–16. doi:10.1038/sj.onc.1206972. PMID   14603251. S2CID   10536264.
11. Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH (Apr 2002). "Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells". Biochem. J. 363 (Pt 1): 195–200. doi:10.1042/0264-6021:3630195. PMC   1222467 . PMID   11903063.

External links

• Human CKAP5 genome location and CKAP5 gene details page in the UCSC Genome Browser .

Further reading

• Basto R, Gergely F, Draviam VM, Ohkura H, Liley K, Raff JW (2007). "Hsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humans". J. Cell Sci. 120 (Pt 7): 1278–87. doi: 10.1242/jcs.000604 . PMID   17376965.
• Kosturko LD, Maggipinto MJ, D'Sa C, Carson JH, Barbarese E (2005). "The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2". Mol. Biol. Cell. 16 (4): 1938–47. doi:10.1091/mbc.E04-08-0709. PMC   1073673 . PMID   15703215.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC   514446 . PMID   15302935.
• Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly". Mol. Biol. Cell. 15 (4): 1580–90. doi:10.1091/mbc.E03-07-0544. PMC   379257 . PMID   14718566.
• Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D (2003). "TACC1-chTOG-Aurora A protein complex in breast cancer". Oncogene. 22 (50): 8102–16. doi:10.1038/sj.onc.1206972. PMID   14603251. S2CID   10536264.
• Gergely F, Draviam VM, Raff JW (2003). "The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells". Genes Dev. 17 (3): 336–41. doi:10.1101/gad.245603. PMC   195983 . PMID   12569123.
• Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC   187542 . PMID   12421765.
• Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH (2002). "Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells". Biochem. J. 363 (Pt 1): 195–200. doi:10.1042/0264-6021:3630195. PMC   1222467 . PMID   11903063.
• Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi: 10.1101/gr.6.9.791 . PMID   8889548.
• Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y (2012). "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells". PLOS ONE. 7 (12): e51442. Bibcode:2012PLoSO...751442N. doi: 10.1371/journal.pone.0051442 . PMC   3520847 . PMID   23251535.
• Zanic M, Widlund PO, Hyman AA, Howard J (2013). "Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels". Nat. Cell Biol. 15 (6): 688–93. doi:10.1038/ncb2744. PMID   23666085. S2CID   3025200.