D-Ala-D-Ala dipeptidase

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D-Ala-D-Ala dipeptidase
Identifiers
EC no. 3.4.13.22
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D-Ala-D-Ala dipeptidase (EC 3.4.13.22, D-alanyl-D-alanine dipeptidase, vanX D-Ala-D-Ala dipeptidase, VanX) is an enzyme. [1] [2] [3] [4] [5] [6] This enzyme catalyses the hydrolysis of the D-alanine dipeptide.

D-Ala-D-Ala + H2O 2 D-Ala

This enzyme is Zn2+-dependent.

References

  1. ↑ Reynolds PE, Depardieu F, Dutka-Malen S, Arthur M, Courvalin P (September 1994). "Glycopeptide resistance mediated by enterococcal transposon Tn1546 requires production of VanX for hydrolysis of D-alanyl-D-alanine". Molecular Microbiology. 13 (6): 1065–70. doi:10.1111/j.1365-2958.1994.tb00497.x. PMID   7854121.
  2. ↑ Wu Z, Wright GD, Walsh CT (February 1995). "Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147". Biochemistry. 34 (8): 2455–63. doi:10.1021/bi00008a008. PMID   7873524.
  3. ↑ McCafferty DG, Lessard IA, Walsh CT (August 1997). "Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX". Biochemistry. 36 (34): 10498–505. doi:10.1021/bi970543u. PMID   9265630.
  4. ↑ Bussiere DE, Pratt SD, Katz L, Severin JM, Holzman T, Park CH (July 1998). "The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance". Molecular Cell. 2 (1): 75–84. doi: 10.1016/s1097-2765(00)80115-x . PMID   9702193.
  5. ↑ Tan AL, Loke P, Sim TS (2002). "Molecular cloning and functional characterisation of VanX, a D-alanyl-D-alanine dipeptidase from Streptomyces coelicolor A3(2)". Research in Microbiology. 153 (1): 27–32. doi: 10.1016/s0923-2508(01)01282-7 . PMID   11881895.
  6. ↑ Matthews ML, Periyannan G, Hajdin C, Sidgel TK, Bennett B, Crowder MW (October 2006). "Probing the reaction mechanism of the D-ala-D-ala dipeptidase, VanX, by using stopped-flow kinetic and rapid-freeze quench EPR studies on the Co(II)-substituted enzyme". Journal of the American Chemical Society. 128 (40): 13050–1. doi:10.1021/ja0627343. PMC   2547881 . PMID   17017774.
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