Beta-peptidyl aminopeptidase

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Beta-peptidyl aminopeptidase
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EC no. 3.4.11.25
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Beta-peptidyl aminopeptidase (EC 3.4.11.25, BapA) is an enzyme. [1] [2] [3] [4] This enzyme catalyses the following chemical reaction:

Cleaves N-terminal beta-homoamino acids from peptides composed of 2 to 6 amino acids

Sphingosinicella xenopeptidilytica strain 3-2W4 could use beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu as only source of carbon and energy.

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Amastatin, also known as 3-amino-2-hydroxy-5-methylhexanoyl-L-valyl-L-valyl-L-aspartic acid, is a naturally occurring, competitive and reversible aminopeptidase inhibitor that was isolated from Streptomyces sp. ME 98-M3. It specifically inhibits leucyl aminopeptidase, alanyl aminopeptidase, bacterial leucyl aminopeptidase, leucyl/cystinyl aminopeptidase (oxytocinase/vasopressinase), and, to a lesser extent, glutamyl aminopeptidase, as well as other aminopeptidases. It does not inhibit arginyl aminopeptidase. Amastatin has been found to potentiate the central nervous system effects of oxytocin and vasopressin in vivo. It also inhibits the degradation of met-enkephalin, dynorphin A, and other endogenous peptides.

References

  1. Heck T, Limbach M, Geueke B, Zacharias M, Gardiner J, Kohler HP, Seebach D (December 2006). "Enzymatic degradation of beta- and mixed alpha,beta-oligopeptides". Chemistry & Biodiversity. 3 (12): 1325–48. doi:10.1002/cbdv.200690136. PMID   17193247. S2CID   38292191.
  2. Geueke B, Namoto K, Seebach D, Kohler HP (September 2005). "A novel beta-peptidyl aminopeptidase (BapA) from strain 3-2W4 cleaves peptide bonds of synthetic beta-tri- and beta-dipeptides". Journal of Bacteriology. 187 (17): 5910–7. doi:10.1128/jb.187.17.5910-5917.2005. PMC   1196161 . PMID   16109932.
  3. Geueke B, Heck T, Limbach M, Nesatyy V, Seebach D, Kohler HP (December 2006). "Bacterial beta-peptidyl aminopeptidases with unique substrate specificities for beta-oligopeptides and mixed beta,alpha-oligopeptides". The FEBS Journal. 273 (23): 5261–72. doi: 10.1111/j.1742-4658.2006.05519.x . PMID   17064315. S2CID   84167461.
  4. Heck T, Kohler HP, Limbach M, Flögel O, Seebach D, Geueke B (September 2007). "Enzyme-catalyzed formation of beta-peptides: beta-peptidyl aminopeptidases BapA and DmpA acting as beta-peptide-synthesizing enzymes". Chemistry & Biodiversity. 4 (9): 2016–30. doi:10.1002/cbdv.200790168. PMID   17886858. S2CID   85123861.