In enzymology, a D-xylulose reductase (EC 1.1.1.9) is an enzyme that is classified as an Oxidoreductase (EC 1) specifically acting on the CH-OH group of donors (EC 1.1.1) that uses NAD+ or NADP+ as an acceptor (EC 1.1.1.9).[1] This enzyme participates in pentose and glucuronate interconversions; a set of metabolic pathways that involve converting pentose sugars and glucuronate into other compounds.
The systematic name of this enzyme class is xylitol:NAD+ 2-oxidoreductase (D-xylulose-forming). Other common names used include:[2]
NAD+-dependent xylitol dehydrogenase
xylitol dehydrogenase* erythritol dehydrogenase (as this enzyme also acts as an L-erythrylose reductase)
2,3-cis-polyol(DPN) dehydrogenase (C3-5)
pentitol-DPN dehydrogenase, and
xylitol-2-dehydrogenase
EC number
An Enzyme Commission (EC) number is a classification identifier given to all enzymes that helps identify their function and relationships to other enzymes.[3] The EC number for D-xylulose reductase is 1.1.1.9, the breakdown is as follows:
EC 1: Oxidoreductase enzymes
EC 1.1.1: An oxidoreductase enzyme that acts on CH-OH group of donors
EC 1.1.1.9: An oxidoreductase enzyme that acts on the CH-OH group of donors that uses NAD+ or NADP+ as an acceptor[1]
Catalyzed reaction
D-xylulose reductase catalyzes the chemical reaction
where xylitol and NAD are the substrates and D-xylulose, NADH and H+ are the products.
NAD+ acts as the coenzyme for the chemical reaction.
The enzyme is a part of a class called short-chain dehydrogenase/reductases (SDRs) enzyme class which are catalyzed by the amino acid tyrosine which acts as an acid or base. Additionally, hydrogen bonding within the enzyme is thought to help with catalysis by changing the surrounding environment to be favorable towards the reaction.[5]
Role in metabolism
In cellular metabolism D-xylulose reductase is essential in various organisms. In bacteria, D-xylulose reductase is the second step of D-xylose metabolism which is necessary cellular growth. First, D-xylose is converted to xylitol which then is converted to D-xylulose when D-xylulose reductase exchanges NAD+ for NADH. D-xylulose then becomes phosphorylated, and proceeds through the rest of the cycle to create α-ketoglutarate which eventually enters the TCA cycle for energy production.[6]
Species distribution
D-xylulose reductase is found in both prokaryotes and eukaryotes; including molds, yeasts, and fungi.
Structure
Only one structure has been solved for this enzyme, under the Protein Data Bank accession code 1ZEM.[5] The enzyme has 262 amino acid residues and is a tetramer. Each monomer has a Rossman fold domain made up of seven β sheets side by side with three short α helices on one end of the sheet and three longer α helices on the other. There are two shorter helices that stay outside of this Rossman fold.
The N-terminal region of the primary sequence has been found to be responsible for the selectivity of binding NAD+, and the active site is located between helices αFG1, αFG2 and the C-terminal end of the β sheet.
Some images of D-xylulose reductase show magnesium bound, this is because the solution used to grow the protein crystals for visualization that were used contained 100mM of MgCl2. However it has been found that D-xylulose reductase is not inhibited by magnesium; it has even been suggested that magnesium may be important for stabilization and formation of the oligomers.[5]
Function
One example of a mold that uses the enzyme is Aspergillus carbonarius; where the enzyme creates an intermediate for the pentose phosphate pathway. D-xylose is converted to xylitol by xylose reductase, then xylitol is converted to xylulose by D-xylulose reductase, afterwards xylulose is converted to xylulose-5-phosphate by xylulokinase and xylulose-5-P then goes into the pentose phosphate pathway for energy and intermediates production.[7]
In yeast, such as Hansenula polymorpha d-xylulose reductase has been found to help ferment xylose into ethanol, however it usually results in an accumulation of xylitol due to the imbalance between cofactor preferences in xylose reductase and xylulose reductase.[8]
In fungi d-xylulose reductase also ferments xylose into ethanol as a result of metabolic conditions (such as anaerobic conditions).[9]
Related Research Articles
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In enzymology, a fructuronate reductase (EC 1.1.1.57) is an enzyme that catalyzes the chemical reaction
In enzymology, a glucuronate reductase (EC 1.1.1.19) is an enzyme that catalyzes the chemical reaction
In enzymology, a glycerate dehydrogenase (EC 1.1.1.29) is an enzyme that catalyzes the chemical reaction
In enzymology, a hydroxypyruvate reductase (EC 1.1.1.81) is an enzyme that catalyzes the chemical reaction
In enzymology, a L-arabinitol 4-dehydrogenase (EC 1.1.1.12) is an enzyme that catalyzes the chemical reaction
In enzymology, a ribitol 2-dehydrogenase (EC 1.1.1.56) is an enzyme that catalyzes the chemical reaction
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In enzymology, a xylulokinase is an enzyme that catalyzes the chemical reaction
D-xylose reductase (EC 1.1.1.307, XylR, XyrA, msXR, dsXR, monospecific xylose reductase, dual specific xylose reductase, NAD(P)H-dependent xylose reductase, xylose reductase) is an enzyme with systematic name xylitol:NAD(P)+ oxidoreductase. This enzyme catalyses the following chemical reaction
References
1 2 Moss G (2023-07-26). "EC 1.1.1.9". Enzyme Nomenclature. International Union of Biochemistry and Molecular Biology (IUBMB). Retrieved 2023-10-01– via School of Physical and Chemical Sciences, Queen Mary, University of London.
↑ Kim D, Woo HM (November 2018). "Deciphering bacterial xylose metabolism and metabolic engineering of industrial microorganisms for use as efficient microbial cell factories". Applied Microbiology and Biotechnology. 102 (22): 9471–9480. doi:10.1007/s00253-018-9353-2. PMID30238140. S2CID52307667.
Chiang C, Knight SG (November 1960). "A new pathway of pentose metabolism". Biochemical and Biophysical Research Communications. 3 (5): 554–559. doi:10.1016/0006-291X(60)90174-1. PMID13692998.
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