EP-B2

Endoprotease B, isoform 2 (EP-B2) is a cysteine protease found in barley. In studies, it has shown promise as a possible therapeutic for coeliac disease. ^[1] Aspergillopepsin from Aspergillus niger has been shown to enhance the effects of EP-B2. ^[2]

References

1. Vora, H.; McIntire, J.; Kumar, P.; Deshpande, M.; Khosla, C. (2007). "A scaleable manufacturing process for pro-EP-B2, a cysteine protease from barley indicated for celiac sprue". Biotechnology and Bioengineering. 98 (1): 177–185. doi:10.1002/bit.21423. PMID   17385743. S2CID   19581223.
2. Ehren, J.; Morón, B.; Martin, E.; Bethune, M.; Gray, G.; Khosla, C.; El-Shemy, H. A. (2009). "A food-grade enzyme preparation with modest gluten detoxification properties". PLOS ONE. 4 (7) e6313. Bibcode:2009PLoSO...4.6313E. doi: 10.1371/journal.pone.0006313 . PMC   2708912 . PMID   19621078.

Further reading

• Bethune, M.; Ribka, E.; Khosla, C.; Sestak, K.; Bozza, P. (2008). "Transepithelial transport and enzymatic detoxification of gluten in gluten-sensitive rhesus macaques". PLOS ONE. 3 (3) e1857. Bibcode:2008PLoSO...3.1857B. doi: 10.1371/journal.pone.0001857 . PMC   2267209 . PMID   18365012.
• Gass, J.; Bethune, M.; Siegel, M.; Spencer, A.; Khosla, C. (2007). "Combination enzyme therapy for gastric digestion of dietary gluten in patients with celiac sprue". Gastroenterology. 133 (2): 472–480. doi: 10.1053/j.gastro.2007.05.028 . PMID   17681168.
• Siegel, M.; Bethune, M.; Gass, J.; Ehren, J.; Xia, J.; Johannsen, A.; Stuge, T.; Gray, G.; Lee, P.; Khosla, C. (2006). "Rational design of combination enzyme therapy for celiac sprue". Chemistry & Biology. 13 (6): 649–658. doi: 10.1016/j.chembiol.2006.04.009 . PMID   16793522.
• Bethune, M.; Strop, P.; Tang, Y.; Sollid, L.; Khosla, C. (2006). "Heterologous expression, purification, refolding, and structural-functional characterization of EP-B2, a self-activating barley cysteine endoprotease". Chemistry & Biology. 13 (6): 637–647. doi: 10.1016/j.chembiol.2006.04.008 . PMID   16793521.
• Gass, J.; Vora, H.; Bethune, M.; Gray, G.; Khosla, C. (2006). "Effect of barley endoprotease EP-B2 on gluten digestion in the intact rat". The Journal of Pharmacology and Experimental Therapeutics. 318 (3): 1178–1186. doi:10.1124/jpet.106.104315. PMID   16757540. S2CID   33662709.