Cathepsin L1

Last updated

CTSL
Protein CTSL1 PDB 1cjl.png
Available structures
PDB Human UniProt search: PDBe RCSB
Identifiers
Aliases CTSL , CATL, CTSL1, MEP, cathepsin L
External IDs OMIM: 116880; HomoloGene: 129366; GeneCards: CTSL; OMA:CTSL - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

n/a

RefSeq (protein)

n/a

Location (UCSC) Chr 9: 87.72 – 87.73 Mb n/a
PubMed search [2] n/a
Wikidata
View/Edit Human

Cathepsin L1 is a protein that in humans is encoded by the CTSL1 gene. [3] [4] [5] The protein is a cysteine cathepsin, a lysosomal cysteine protease that plays a major role in intracellular protein catabolism. [6] [7] [8] [9]

Function

Cathepsin L1 is a member of the Peptidase C1 (cathepsin) MEROPS family, which plays an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. [10] Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. [5]

Viral entry

Cleavage of the SARS-CoV-2 S2 spike protein required for viral entry into cells can be accomplished by proteases TMPRSS2 located on the cell membrane, or by cathepsins (primarily cathepsin L) in endolysosomes. [11] Hydroxychloroquine inhibits the action of cathepsin L in endolysosomes, but because cathepsin L cleavage is minor compared to TMPRSS2 cleavage, hydroxychloroquine does little to inhibit SARS-CoV-2 infection. [11]

Inflammation

Although Cathepsin L is usually characterized as a lysosomal protease, it can be secreted, resulting in pathological inflammation. [12] Cathepsin L and other cysteine cathepsins tend to be secreted by macrophages and other tissue-invading immune cells when causing pathological inflammation. [13]

Interactions

CTSL1 has been shown to interact with Cystatin A. [14] [15]

Distribution

Cathepsin L has been reported in many organisms including fish, [16] birds, mammals, and sponges. [17]

See also

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000135047 Ensembl, May 2017
  2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. Chauhan SS, Popescu NC, Ray D, Fleischmann R, Gottesman MM, Troen BR (Feb 1993). "Cloning, genomic organization, and chromosomal localization of human cathepsin L". J Biol Chem. 268 (2): 1039–45. doi: 10.1016/S0021-9258(18)54038-2 . PMID   8419312.
  4. Joseph LJ, Chang LC, Stamenkovich D, Sukhatme VP (Jun 1988). "Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts". J Clin Invest. 81 (5): 1621–9. doi:10.1172/JCI113497. PMC   442598 . PMID   2835398.
  5. 1 2 "Entrez Gene: CTSL1 cathepsin L1".
  6. Barrett AJ, Kirschke H (1981). "Cathepsin B, cathepsin H, and cathepsin L". Proteolytic Enzymes, Part C. Methods in Enzymology. Vol. 80 Pt C. pp. 535–561. doi:10.1016/s0076-6879(81)80043-2. ISBN   9780121819804. PMID   7043200.
  7. Barrett AJ, Buttle DJ, Mason RW (1988). "Lysosomal cysteine proteinases". ISI Atlas of Science. Biochemistry. 1: 256–260.
  8. Joseph LJ, Chang LC, Stamenkovich D, Sukhatme VP (May 1988). "Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts". The Journal of Clinical Investigation. 81 (5): 1621–1629. doi:10.1172/JCI113497. PMC   442598 . PMID   2835398.
  9. Kirschke H, Wikstrom P, Shaw E (February 1988). "Active center differences between cathepsins L and B: the S1 binding region". FEBS Letters. 228 (1): 128–130. Bibcode:1988FEBSL.228..128K. doi: 10.1016/0014-5793(88)80600-8 . PMID   3342870.
  10. Dickinson DP (2002). "Cysteine Peptidases of Mammals: Their Biological Roles and Potential Effects in the Oral Cavity and Other Tissues in Health and Disease". Critical Reviews in Oral Biology and Medicine. 13 (3): 238–75. doi:10.1177/154411130201300304. PMID   12090464.
  11. 1 2 Jackson CB, Farzan M, Chen B, Choe H (January 2022). "Mechanisms of SARS-CoV-2 entry into cells". Nature Reviews. Molecular Cell Biology. 23 (1): 3–20. doi:10.1038/s41580-021-00418-x. PMC   8491763 . PMID   34611326.
  12. Gomes CP, Fernandes DE, Casimiro F, da Mata GF, Passos MT, Varela P, et al. (2022). "Cathepsin L in COVID-19: From Pharmacological Evidences to Genetics". Frontiers in Cellular and Infection Microbiology. 10: 589505. doi: 10.3389/fcimb.2020.589505 . PMC   7753008 . PMID   33364201.
  13. Berdowska I, Matusiewicz M (October 2021). "Cathepsin L, transmembrane peptidase/serine subfamily member 2/4, and other host proteases in COVID-19 pathogenesis - with impact on gastrointestinal tract". World Journal of Gastroenterology. 27 (39): 6590–6600. doi: 10.3748/wjg.v27.i39.6590 . PMC   8554394 . PMID   34754154.
  14. Majerle A, Jerala Roman (Sep 2003). "Protein inhibitors form complexes with procathepsin L and augment cleavage of the propeptide". Arch. Biochem. Biophys. 417 (1): 53–8. doi:10.1016/S0003-9861(03)00319-9. ISSN   0003-9861. PMID   12921779.
  15. Estrada S, Nycander M, Hill N J, Craven C J, Waltho J P, Björk I (May 1998). "The role of Gly-4 of human cystatin A (stefin A) in the binding of target proteinases. Characterization by kinetic and equilibrium methods of the interactions of cystatin A Gly-4 mutants with papain, cathepsin B, and cathepsin L". Biochemistry. 37 (20): 7551–60. doi:10.1021/bi980026r. ISSN   0006-2960. PMID   9585570.
  16. Venkatesh K, Prasanth B, Rajesh P, Annie JG, Mukesh P, Jesu A (2014). "A murrel cysteine protease, cathepsin L: bioinformatics characterization, gene expression and proteolytic activity". Biologia. 39 (3): 395–406. Bibcode:2014Biolg..69..395K. doi: 10.2478/s11756-013-0326-8 .
  17. Sevenich L, Pennacchio LA, Peters C, Reinheckel T (July 2006). "Human cathepsin L rescues the neurodegeneration and lethality in cathepsin B/L double-deficient mice". Biological Chemistry. 387 (7): 885–91. doi:10.1515/BC.2006.112. OSTI   927243. PMID   16913838. S2CID   27739485.

Further reading