Entamoeba gingivalis

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Entamoeba gingivalis
Parasite130116-1-olm Entamoeba gingivalis microscopy.tif
Entamoeba gingivalis from periodontal pocket [1]
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Phylum: Amoebozoa
Family: Entamoebidae
Genus: Entamoeba
Species:
E. gingivalis
Binomial name
Entamoeba gingivalis
Gros, 1849
Entamoeba gingivalis from aggressive periodontal disease patient biofilm using phase contrast microscope 1000x. It is recognizable through its dense core in the middle, formed by a central point encircled by a circular halo and surrounded by bigger phagosomes inside a greyish cytoplasm. Entamoeba gingivalis.jpg
Entamoeba gingivalis from aggressive periodontal disease patient biofilm using phase contrast microscope 1000x. It is recognizable through its dense core in the middle, formed by a central point encircled by a circular halo and surrounded by bigger phagosomes inside a greyish cytoplasm.

Entamoeba gingivalis is an opportunistic Amoebozoa [ citation needed ] (reported by some as an effect of disease; not a cause [hence status as a commensal]) [2] [3] [4] and is the first amoeba in humans to be described.

Contents

It is found in the mouth [5] inside the gingival pocket biofilm near the base of the teeth, and in periodontal pockets. [1] Entamoeba gingivalis is found in 95% of people with gum disease and rarely in people with healthy gums. [6] [7] Cyst formation is not present; therefore transmission is direct from one person to another by kissing, or by sharing eating utensils. Only the trophozoites are formed and the size is usually 20 micrometers to 150 micrometers in diameter. Entamoeba gingivalis have pseudopodia that allow them to move quickly and phagocytise the nucleus of polynuclear neutrophils by exonucleophagy in periodontal disease. [8] Their spheroid nucleus is 2 micrometers to 4 micrometers in diameter and contains a small central endosome. There are numerous food vacuoles, which consists mostly of phagocitised PMN nucleus, blood cells, and bacteria. It also causes pyorrhoea.

Media

From the top of the image, Entamoeba gingivalis is sipping a white cell nucleus, imperceptibly through negative suction apparently. Halfway through the process, it starts enveloping its prey in the middle, to better digest it in a future phagosome.

The main activity of the amoeba Entamoeba gingivalis in the infected periodontal crevices, besides moving, consists in feeding on the nucleus of white blood cells. The amoeba penetrates into the cytoplasm to reach the nucleus and literally suctions its contents via the negative pressure of the pseudopod. The food so gulped down is gradually digested inside the endoplasm. Phagocytosis can sometimes continue for more than 20 polynuclear neutrophil nuclei. This activity leaves a denucleated cell, unable to achieve either its NETS activity or its preprogrammed apoptosis. It will release PMN-uncontrolled proteolytic enzymes on surrounding tissues and could be considered a pathogen from this vampirising activity.

Related Research Articles

<i>Entamoeba</i> Genus of internal parasites

Entamoeba is a genus of Amoebozoa found as internal parasites or commensals of animals. In 1875, Fedor Lösch described the first proven case of amoebic dysentery in St. Petersburg, Russia. He referred to the amoeba he observed microscopically as Amoeba coli; however, it is not clear whether he was using this as a descriptive term or intended it as a formal taxonomic name. The genus Entamoeba was defined by Casagrandi and Barbagallo for the species Entamoeba coli, which is known to be a commensal organism. Lösch's organism was renamed Entamoeba histolytica by Fritz Schaudinn in 1903; he later died, in 1906, from a self-inflicted infection when studying this amoeba. For a time during the first half of the 20th century the entire genus Entamoeba was transferred to Endamoeba, a genus of amoebas infecting invertebrates about which little is known. This move was reversed by the International Commission on Zoological Nomenclature in the late 1950s, and Entamoeba has stayed 'stable' ever since.

<span class="mw-page-title-main">Periodontal disease</span> Medical condition

Periodontal disease, also known as gum disease, is a set of inflammatory conditions affecting the tissues surrounding the teeth. In its early stage, called gingivitis, the gums become swollen and red and may bleed. It is considered the main cause of tooth loss for adults worldwide. In its more serious form, called periodontitis, the gums can pull away from the tooth, bone can be lost, and the teeth may loosen or fall out. Halitosis may also occur.

<span class="mw-page-title-main">Calculus (dental)</span> Form of hardened dental plaque

In dentistry, calculus or tartar is a form of hardened dental plaque. It is caused by precipitation of minerals from saliva and gingival crevicular fluid (GCF) in plaque on the teeth. This process of precipitation kills the bacterial cells within dental plaque, but the rough and hardened surface that is formed provides an ideal surface for further plaque formation. This leads to calculus buildup, which compromises the health of the gingiva (gums). Calculus can form both along the gumline, where it is referred to as supragingival, and within the narrow sulcus that exists between the teeth and the gingiva, where it is referred to as subgingival.

Periodontology or periodontics is the specialty of dentistry that studies supporting structures of teeth, as well as diseases and conditions that affect them. The supporting tissues are known as the periodontium, which includes the gingiva (gums), alveolar bone, cementum, and the periodontal ligament. A periodontist is a dentist that specializes in the prevention, diagnosis and treatment of periodontal disease and in the placement of dental implants.

Dental plaque is a biofilm of microorganisms that grows on surfaces within the mouth. It is a sticky colorless deposit at first, but when it forms tartar, it is often brown or pale yellow. It is commonly found between the teeth, on the front of teeth, behind teeth, on chewing surfaces, along the gumline (supragingival), or below the gumline cervical margins (subgingival). Dental plaque is also known as microbial plaque, oral biofilm, dental biofilm, dental plaque biofilm or bacterial plaque biofilm. Bacterial plaque is one of the major causes for dental decay and gum disease.

<span class="mw-page-title-main">Oral irrigator</span> Dental care device

An oral irrigator is a home dental care device which uses a stream of high-pressure pulsating water intended to remove dental plaque and food debris between teeth and below the gum line. Regular use of an oral irrigator is believed to improve gingival health. The devices may also provide easier cleaning for braces and dental implants. However, more research is needed to confirm plaque biofilm removal and effectiveness when used by patients with special oral or systemic health needs.

<span class="mw-page-title-main">Gingival sulcus</span> Space between tooth and gums

The gingival sulcus is an area of potential space between a tooth and the surrounding gingival tissue and is lined by sulcular epithelium. The depth of the sulcus is bounded by two entities: apically by the gingival fibers of the connective tissue attachment and coronally by the free gingival margin. A healthy sulcular depth is three millimeters or less, which is readily self-cleansable with a properly used toothbrush or the supplemental use of other oral hygiene aids.

Porphyromonas gingivalis belongs to the phylum Bacteroidota and is a nonmotile, Gram-negative, rod-shaped, anaerobic, pathogenic bacterium. It forms black colonies on blood agar.

<span class="mw-page-title-main">Gingival and periodontal pocket</span>

Gingival and periodontal pockets are dental terms indicating the presence of an abnormal depth of the gingival sulcus near the point at which the gingival tissue contacts the tooth.

<span class="mw-page-title-main">Scaling and root planing</span> Dental procedure

Scaling and root planing, also known as conventional periodontal therapy, non-surgical periodontal therapy or deep cleaning, is a procedure involving removal of dental plaque and calculus and then smoothing, or planing, of the (exposed) surfaces of the roots, removing cementum or dentine that is impregnated with calculus, toxins, or microorganisms, the agents that cause inflammation. It is a part of non-surgical periodontal therapy. This helps to establish a periodontium that is in remission of periodontal disease. Periodontal scalers and periodontal curettes are some of the tools involved.

The junctional epithelium (JE) is that epithelium which lies at, and in health also defines, the base of the gingival sulcus. The probing depth of the gingival sulcus is measured by a calibrated periodontal probe. In a healthy-case scenario, the probe is gently inserted, slides by the sulcular epithelium (SE), and is stopped by the epithelial attachment (EA). However, the probing depth of the gingival sulcus may be considerably different from the true histological gingival sulcus depth.

Treponema denticola is a Gram-negative, obligate anaerobic, motile and highly proteolytic spirochete bacterium. It is one of four species of oral spirochetes to be reliably cultured, the others being Treponema pectinovorum, Treponema socranskii and Treponema vincentii. T. denticola dwells in a complex and diverse microbial community within the oral cavity and is highly specialized to survive in this environment. T. denticola is associated with the incidence and severity of human periodontal disease. Treponema denticola is one of three bacteria that form the Red Complex, the other two being Porphyromonas gingivalis and Tannerella forsythia. Together they form the major virulent pathogens that cause chronic periodontitis. Having elevated T. denticola levels in the mouth is considered one of the main etiological agents of periodontitis. T. denticola is related to the syphilis-causing obligate human pathogen, Treponema pallidum subsp. pallidum. It has also been isolated from women with bacterial vaginosis.

<span class="mw-page-title-main">Gingivitis</span> Inflammation of the gums

Gingivitis is a non-destructive disease that causes inflammation of the gums; ulitis is an alternative term. The most common form of gingivitis, and the most common form of periodontal disease overall, is in response to bacterial biofilms that are attached to tooth surfaces, termed plaque-induced gingivitis. Most forms of gingivitis are plaque-induced.

Gingival disease is a term used to group the diseases that affect the gingiva(gums). The most common gingival disease is gingivitis, the earliest stage of gingival-related diseases. Gingival disease encompasses all the conditions that surround the gums, this includes plaque-induced gingivitis, non-dental biofilm plaque-induced gingivitis, and periodontal diseases.

<span class="mw-page-title-main">Debridement (dental)</span> Removal of plaque and calculus from teeth

In dentistry, debridement refers to the removal by dental cleaning of accumulations of plaque and calculus (tartar) in order to maintain dental health. Debridement may be performed using ultrasonic instruments, which fracture the calculus, thereby facilitating its removal, as well as hand tools, including periodontal scaler and curettes, or through the use of chemicals such as hydrogen peroxide.

<span class="mw-page-title-main">Periodontal abscess</span> Medical condition

A periodontal abscess, is a localized collection of pus within the tissues of the periodontium. It is a type of dental abscess. A periodontal abscess occurs alongside a tooth, and is different from the more common periapical abscess, which represents the spread of infection from a dead tooth. To reflect this, sometimes the term "lateral (periodontal) abscess" is used. In contrast to a periapical abscess, periodontal abscesses are usually associated with a vital (living) tooth. Abscesses of the periodontium are acute bacterial infections classified primarily by location.

Chronic periodontitis is one of the seven categories of periodontitis as defined by the American Academy of Periodontology 1999 classification system. Chronic periodontitis is a common disease of the oral cavity consisting of chronic inflammation of the periodontal tissues that is caused by the accumulation of profuse amounts of dental plaque. Periodontitis initially begins as gingivitis and can progress onto chronic and subsequent aggressive periodontitis according to the 1999 classification.

Aggressive periodontitis describes a type of periodontal disease and includes two of the seven classifications of periodontitis as defined by the 1999 classification system:

  1. Localized aggressive periodontitis (LAP)
  2. Generalized aggressive periodontitis (GAP)
<i>Trichomonas tenax</i> Species of single-celled organism

Trichomonas tenax, or oral trichomonas, is a species of Trichomonas commonly found in the oral cavity of humans. Routine hygiene is generally not sufficient to eliminate the parasite, hence its Latin name, meaning "tenacious". The parasite is frequently encountered in periodontal infections, affecting more than 50% of the population in some areas, but it is usually considered insignificant. T. tenax is generally not found on the gums of healthy patients. It is known to play a pathogenic role in necrotizing ulcerative gingivitis and necrotizing ulcerative periodontitis, worsening preexisting periodontal disease. This parasite is also implicated in some chronic lung diseases; in such cases, removal of the parasite is sufficient to allow recovery.

References

  1. 1 2 Bonner M, Amard V, Bar-Pinatel C, Charpentier F, Chatard JM, Desmuyck Y, et al. (2014). "Detection of the amoeba Entamoeba gingivalis in periodontal pockets". Parasite. 21: 30. doi:10.1051/parasite/2014029. PMC   4077299 . PMID   24983705.
  2. Jian B, Kolansky AS, Baloach ZW, Gupta PK (September 2008). "Entamoeba gingivalis pulmonary abscess - diagnosed by fine needle aspiration". CytoJournal. 5: 12. doi: 10.4103/1742-6413.43179 . PMC   2669679 . PMID   19495399.
  3. Lyons T, Sholten T, Palmer JC (October 1980). "Oral amoebiasis: a new approach for the general practitioner in the diagnosis and treatment of periodontal disease". Oral Health. 70 (10): 39–41, 108, 110. PMID   6950337.
  4. Lyons T. Introduction to protozoa and fungi in periodontal disease. Trevor Lyons publications, Ontario, Canada 1989. ISBN   0-9693950-0-0
  5. Prieto-Prieto J, Calvo A (2004). "Microbiological basis of oral infections and sensitivity to antibiotics". Medicina Oral, Patologia Oral y Cirugia Bucal. 9 Suppl: 15–8, 11–4. PMID   15580129.
  6. Kofoid CA, Hinshaw HC, Johnstone HG (1929). "Animal Parasites of the Mouth and Their Relation to Dental Disease**From the Protozoological Section of the California Stomatological Research Group and the Department of Zoology of the University of California, under the direction of Prof. Charles A. Kofoid, aided by grants from the Carnegie Corporation, from the American Dental Association and from the Associated Laboratories of San Francisco". The Journal of the American Dental Association. 16 (8): 1436–1455. doi:10.14219/jada.archive.1929.0207.
  7. Trim RD, Skinner MA, Farone MB, Dubois JD, Newsome AL (September 2011). "Use of PCR to detect Entamoeba gingivalis in diseased gingival pockets and demonstrate its absence in healthy gingival sites". Parasitology Research. 109 (3): 857–64. doi:10.1007/s00436-011-2312-9. PMID   21400116.
  8. Bonner M (2013). To Kiss or Not to Kiss. Amyris Editions. ISBN   978-28755-2016-6.

Further reading