Galdieria sulphuraria

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Galdieria sulphuraria
Scientific classification OOjs UI icon edit-ltr.svg
(unranked): Archaeplastida
Division: Rhodophyta
Class: Cyanidiophyceae
Order: Cyanidiales
Family: Galdieriaceae
Genus: Galdieria
Species:
G. sulphuraria
Binomial name
Galdieria sulphuraria
Merola et al., 1981 [1]

Galdieria sulphuraria is an extremophilic unicellular species of red algae. It is the type species of the genus Galdieria . [2] It is known for its broad metabolic capacities, including photosynthesis and heterotrophic growth on over 50 different extracellular carbon sources. The members of the class Cyanidiophyceae are among the most acidophilic known photosynthetic organisms, and the growth conditions of G. sulphurariapH between 0 and 4, and temperatures up to 56 °C – are among the most extreme known for eukaryotes. Analysis of its genome suggests that its thermoacidophilic adaptations derive from horizontal gene transfer from archaea and bacteria, another rarity among eukaryotes. [3]

Contents

History and taxonomy

Published descriptions of thermoacidophilic unicellular algae date to the mid-19th century. The earliest description of an organism corresponding to the modern G. sulphuraria was published in 1899 by an Italian scientist, A. Galdieri, who gave it the name Pleurococcus sulphurarius. The taxonomy of thermoacidophilic algae was revised in 1981, which introduced the genus Galdieria and gave the organism its modern designation. [1] [4] G. sulphuraria is the type species for this genus. [1] [2]

The group to which G. sulphuraria belongs, the Cyanidiophyceae, is the most deeply branching subgroup of the rhodophyta (red algae), meaning they were the earliest to diverge in the evolutionary history of this group. [5]

Metabolism

G. sulphuraria is noted for its extreme metabolic flexibility: it is capable of photosynthesis and can also grow heterotrophically on a wide variety of carbon sources, including diverse carbohydrates. Over 50 different carbon sources that support growth have been reported. [6] [7] [8] Careful measurements of its growth patterns under laboratory conditions suggest that it is not a true mixotroph capable of using both energy sources at the same time; rather, it prefers heterotrophic growth conditions and downregulates photosynthesis after extended exposure to extracellular carbon sources. [9] Analysis of the G. sulphuraria photosystem I complex, a key photosynthetic component, suggests a structure intermediate between the homologous complexes in cyanobacteria and plants. [8]

Although most red algae use floridean starch as a storage glucan, G. sulphuraria uses a highly unusual form of glycogen which is among the most highly branched glycogens known, has very short branch lengths, and forms particles of unusually low molecular weight. These properties are believed to be metabolic adaptations to extreme environmental conditions, although the precise mechanism is unclear. [10]

Habitat and ecology

G. sulphuraria is unusual for a eukaryote in being thermoacidophilic – that is, capable of growing at both high temperature and low pH. It grows well in a pH range of 0–4 and at temperatures up to 56 °C, [9] close to the approximately 60 °C sometimes cited as the likely maximum for eukaryotic life. [11] [12] It is also highly tolerant of high salt concentrations and of toxic metals. It is found in naturally acidic hot springs, in solfataric environments, and in polluted environments; [3] It is also found in endolithic ecosystems, where light is scarce and its heterotrophic metabolic capacities are particularly important. [13] [14] [15] Laboratory tests indicate that it is capable of actively acidifying its environment. [9]

Genome

The G. sulphuraria genome contains evidence of extensive horizontal gene transfer (HGT) from thermoacidophilic archaea and bacteria, explaining the origin of its adaptation to this environment. At least 5% of its proteome is likely to be derived from HGT. [3] This is highly unusual for a eukaryote; relatively few well-substantiated examples exist of HGT from prokaryotes to eukaryotes. [16]

The genome of its mitochondria is also exceptionally small and has a very high GC skew, while the genome of its plastids is of normal size but contains an unusual number of stem-loop structures. Both of these properties are proposed to be adaptations for the organism's polyextremophilic environment. [17] By comparison to Cyanidioschyzon merolae – a unicellular thermoacidophilic red alga that is obligately photoautotrophic – the G. sulphuraria genome contains a large number of genes associated with carbohydrate metabolism and cross-membrane transport. [18]

Biotechnology

Because of its ability to tolerate extreme environments and grow under a wide variety of conditions, G. sulphuraria has been considered for use in bioremediation projects. For example, it has been tested for the ability to recover precious metals, [19] recover rare-earth metals, [20] and remove phosphorus and nitrogen [21] from various waste streams.

It is also an interesting source of proteins and especially phycocianin. The phycocianin produced by this specie is interesting since it is thermoresistant and acidotestant, two interesting properties for food application.

Related Research Articles

<span class="mw-page-title-main">Chlorophyta</span> Phylum of green algae

Chlorophyta is a taxon of green algae informally called chlorophytes. The name is used in two very different senses, so care is needed to determine the use by a particular author. In older classification systems, it is a highly paraphyletic group of all the green algae within the green plants (Viridiplantae) and thus includes about 7,000 species of mostly aquatic photosynthetic eukaryotic organisms. In newer classifications, it is the sister clade of the streptophytes/charophytes. The clade Streptophyta consists of the Charophyta in which the Embryophyta emerged. In this latter sense the Chlorophyta includes only about 4,300 species. About 90% of all known species live in freshwater. Like the land plants, green algae contain chlorophyll a and chlorophyll b and store food as starch in their plastids.

<span class="mw-page-title-main">Cyanobacteria</span> Phylum of photosynthesising prokaryotes

Cyanobacteria, also called Cyanophyta, are a phylum of gram-negative bacteria that obtain energy via photosynthesis. The name cyanobacteria refers to their color, which similarly forms the basis of cyanobacteria's common name, blue-green algae, although they are not usually scientifically classified as algae. They appear to have originated in a freshwater or terrestrial environment. Sericytochromatia, the proposed name of the paraphyletic and most basal group, is the ancestor of both the non-photosynthetic group Melainabacteria and the photosynthetic cyanobacteria, also called Oxyphotobacteria.

<span class="mw-page-title-main">Plastid</span> Plant cell organelles that perform photosynthesis and store starch

The plastid is a membrane-bound organelle found in the cells of plants, algae, and some other eukaryotic organisms. They are considered to be intracellular endosymbiotic cyanobacteria. Examples include chloroplasts, chromoplasts, and leucoplasts.

<span class="mw-page-title-main">Horizontal gene transfer</span> Type of nonhereditary genetic change

Horizontal gene transfer (HGT) or lateral gene transfer (LGT) is the movement of genetic material between unicellular and/or multicellular organisms other than by the ("vertical") transmission of DNA from parent to offspring (reproduction). HGT is an important factor in the evolution of many organisms. HGT is influencing scientific understanding of higher order evolution while more significantly shifting perspectives on bacterial evolution.

<span class="mw-page-title-main">Brown algae</span> Large group of multicellular algae, comprising the class Phaeophyceae

Brown algae, comprising the class Phaeophyceae, are a large group of multicellular algae, including many seaweeds located in colder waters within the Northern Hemisphere. Brown algae are the major seaweeds of the temperate and polar regions. They are dominant on rocky shores throughout cooler areas of the world. Most brown algae live in marine environments, where they play an important role both as food and as a potential habitat. For instance, Macrocystis, a kelp of the order Laminariales, may reach 60 m (200 ft) in length and forms prominent underwater kelp forests. Kelp forests like these contain a high level of biodiversity. Another example is Sargassum, which creates unique floating mats of seaweed in the tropical waters of the Sargasso Sea that serve as the habitats for many species. Many brown algae, such as members of the order Fucales, commonly grow along rocky seashores. Some members of the class, such as kelps, are used by humans as food.

<span class="mw-page-title-main">Unicellular organism</span> Organism that consists of only one cell

A unicellular organism, also known as a single-celled organism, is an organism that consists of a single cell, unlike a multicellular organism that consists of multiple cells. Organisms fall into two general categories: prokaryotic organisms and eukaryotic organisms. Most prokaryotes are unicellular and are classified into bacteria and archaea. Many eukaryotes are multicellular, but some are unicellular such as protozoa, unicellular algae, and unicellular fungi. Unicellular organisms are thought to be the oldest form of life, with early protocells possibly emerging 3.8–4.0 billion years ago.

<span class="mw-page-title-main">Green algae</span> Paraphyletic group of autotrophic eukaryotes in the clade Archaeplastida

The green algae are a group consisting of the Prasinodermophyta and its unnamed sister which contains the Chlorophyta and Charophyta/Streptophyta. The land plants (Embryophytes) have emerged deep in the Charophyte alga as sister of the Zygnematophyceae. Since the realization that the Embryophytes emerged within the green algae, some authors are starting to include them. The completed clade that includes both green algae and embryophytes is monophyletic and is referred to as the clade Viridiplantae and as the kingdom Plantae. The green algae include unicellular and colonial flagellates, most with two flagella per cell, as well as various colonial, coccoid and filamentous forms, and macroscopic, multicellular seaweeds. There are about 22,000 species of green algae. Many species live most of their lives as single cells, while other species form coenobia (colonies), long filaments, or highly differentiated macroscopic seaweeds.

<i>Chlamydomonas reinhardtii</i> Species of alga

Chlamydomonas reinhardtii is a single-cell green alga about 10 micrometres in diameter that swims with two flagella. It has a cell wall made of hydroxyproline-rich glycoproteins, a large cup-shaped chloroplast, a large pyrenoid, and an eyespot that senses light.

<span class="mw-page-title-main">Thermoacidophile</span>

A thermoacidophile is an extremophilic microorganism that is both thermophilic and acidophilic; i.e., it can grow under conditions of high temperature and low pH. The large majority of thermoacidophiles are archaea or bacteria, though occasional eukaryotic examples have been reported. Thermoacidophiles can be found in hot springs and solfataric environments, within deep sea vents, or in other environments of geothermal activity. They also occur in polluted environments, such as in acid mine drainage.

<span class="mw-page-title-main">Protist</span> Eukaryotes other than animals, plants, fungi

A protist is any eukaryotic organism that is not an animal, plant, or fungus. Protists, along with other eukaryotes, all descend from the last eukaryotic common ancestor. Protists do not form a natural group, or clade; any unicellular eukaryote may be described as a protist, in addition to some cases of multicellular protists such as slime molds, brown algae and xenophyophorean forams. Protists represent an extremely large, undiscovered diversity in the process of being defined. The study of protists is termed protistology.

<span class="mw-page-title-main">Red algae</span> Division of archaeplastids

Red algae, or Rhodophyta, are one of the oldest groups of eukaryotic algae. The Rhodophyta comprises one of the largest phyla of algae, containing over 7,000 currently recognized species with taxonomic revisions ongoing. The majority of species (6,793) are found in the Florideophyceae (class), and mostly consist of multicellular, marine algae, including many notable seaweeds. Red algae are abundant in marine habitats but relatively rare in freshwaters. Approximately 5% of red algae species occur in freshwater environments, with greater concentrations found in warmer areas. Except for two coastal cave dwelling species in the asexual class Cyanidiophyceae, there are no terrestrial species, which may be due to an evolutionary bottleneck in which the last common ancestor lost about 25% of its core genes and much of its evolutionary plasticity.

<span class="mw-page-title-main">Eukaryote</span> Domain of life whose cells have nuclei

Eukaryota, whose members are known as eukaryotes, is a diverse domain of organisms whose cells have a nucleus. All animals, plants, fungi, and many unicellular organisms are eukaryotes. They constitute a major group of living things, along with the two groups of prokaryotes, the Bacteria and the Archaea.

<i>Cyanidioschyzon</i> Species of alga

Cyanidioschyzon merolae is a small (2μm), club-shaped, unicellular haploid red alga adapted to high sulfur acidic hot spring environments. The cellular architecture of C. merolae is extremely simple, containing only a single chloroplast and a single mitochondrion and lacking a vacuole and cell wall. In addition, the cellular and organelle divisions can be synchronized. For these reasons, C. merolae is considered an excellent model system for study of cellular and organelle division processes, as well as biochemistry and structural biology. The organism's genome was the first full algal genome to be sequenced in 2004; its plastid was sequenced in 2000 and 2003, and its mitochondrion in 1998. The organism has been considered the simplest of eukaryotic cells for its minimalist cellular organization.

<span class="mw-page-title-main">Picozoa</span> Phylum of marine unicellular heterotrophic eukaryotes

Picozoa, Picobiliphyta, Picobiliphytes, or Biliphytes are protists of a phylum of marine unicellular heterotrophic eukaryotes with a size of less than about 3 micrometers. They were formerly treated as eukaryotic algae and the smallest member of photosynthetic picoplankton before it was discovered they do not perform photosynthesis. The first species identified therein is Picomonas judraskeda. They probably belong in the Archaeplastida as sister of the Rhodophyta.

Picrophilus torridus is a species of Archaea described in 1996. Picrophilus torridus was found in soil near a hot spring in Hokkaido, Japan. The pH of the soil was less than 0.5. P. torridus also has one of the smallest genomes found among organisms that are free-living and are non-parasitic and a high coding density, meaning that the majority of its genes are coding regions and provide instructions for building proteins. The current research suggests the two hostile conditions favored by P. torridus have exerted selective pressure towards having a small and compact genome, which is less likely to be damaged by the harsh environment.

<i>Cyanothece</i> Genus of bacteria

Cyanothece is a genus of unicellular, diazotrophic, oxygenic photosynthesizing cyanobacteria.

<span class="mw-page-title-main">Floridean starch</span> Type of storage glucan

Floridean starch is a type of a storage glucan found in glaucophytes and in red algae, in which it is usually the primary sink for fixed carbon from photosynthesis. It is found in grains or granules in the cell's cytoplasm and is composed of an α-linked glucose polymer with a degree of branching intermediate between amylopectin and glycogen, though more similar to the former. The polymers that make up floridean starch are sometimes referred to as "semi-amylopectin".

<span class="mw-page-title-main">Marine primary production</span>

Marine primary production is the chemical synthesis in the ocean of organic compounds from atmospheric or dissolved carbon dioxide. It principally occurs through the process of photosynthesis, which uses light as its source of energy, but it also occurs through chemosynthesis, which uses the oxidation or reduction of inorganic chemical compounds as its source of energy. Almost all life on Earth relies directly or indirectly on primary production. The organisms responsible for primary production are called primary producers or autotrophs.

Galdieria is a genus of red algae belonging to the family Galdieriaceae. It was created by an Italian botanist Aldo Merola in 1981 for the identification from the species of Cyanidium.

<i>Galdieria partita</i> Species of red algae

Galdieria partita is a species of extremophilic red algae that lives in acidic hot springs. It is the only unicellular species of red algae known to reproduce sexually. It was discovered in 1894 by Josephine Elizabeth Tilden from Yellowstone National Park in the western United States. Originally described as a specides of green algae, Chroococcus varium, its scientific name and taxonomic position were revised several times. In 1959, Mary Belle Allen produced the pure culture which has been distributed as the "Allen strain".

References

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