Deinococcota

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Deinococcota
Deinococcus radiodurans.jpg
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Domain: Bacteria
Subkingdom: Negibacteria
Phylum: Deinococcota
Weisburg et al. 2021 [1]
Class: Deinococci
Garrity and Holt 2002 [2]
Orders & families
Synonyms
  • "Deinobacteria" Cavalier-Smith 2006
  • "Deinococcobacteria" Margulis & Schwartz 1998
  • "Deinococcaeota" Oren et al. 2015
  • "Deinococcota" Whitman et al. 2018
  • "Deinococcus–Thermus" Weisburg et al. 1989
  • "Hadobacteria" Cavalier-Smith 2006 [3]
  • "Xenobacteria"

Deinococcota (synonym, Deinococcus-Thermus) is a phylum of bacteria with a single class, Deinococci, that are highly resistant to environmental hazards, also known as extremophiles. [4] These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria. [5] [6] [7]

Contents

Taxonomy

The phylum Deinococcota consists of a single class ( Deinococci ) and two orders:

Though these two groups evolved from a common ancestor, the two mechanisms of resistance appear to be largely independent. [11] [15]

Molecular signatures

Molecular signatures in the form of conserved signature indels (CSIs) and proteins (CSPs) have been found that are uniquely shared by all members belonging to the Deinococcota phylum. [4] [11] These CSIs and CSPs are distinguishing characteristics that delineate the unique phylum from all other bacterial organisms, and their exclusive distribution is parallel with the observed differences in physiology. CSIs and CSPs have also been found that support order and family-level taxonomic rankings within the phylum. Some of the CSIs found to support order level distinctions are thought to play a role in the respective extremophilic characteristics. [11] The CSIs found in DNA-directed RNA polymerase subunit beta and DNA topoisomerase I in Thermales species may be involved in thermophilicity, [16] while those found in Excinuclease ABC, DNA gyrase, and DNA repair protein RadA in Deinococcales species may be associated with radioresistance. [17] Two CSPs that were found uniquely for all members belonging to the Deinococcus genus are well characterized and are thought to play a role in their characteristic radioresistant phenotype. [11] These CSPs include the DNA damage repair protein PprA the single-stranded DNA-binding protein DdrB.

Additionally, some genera within this group, including Deinococcus , Thermus , and Meiothermus , also have molecular signatures that demarcate them as individual genera, inclusive of their respective species, providing a means to distinguish them from the rest of the group and all other bacteria. [11] CSIs have also been found specific for Truepera radiovictrix .

Phylogeny

16S rRNA based LTP_08_2023 [18] [19] [20] 120 marker proteins based GTDB 08-RS214 [21] [22] [23]
"Deinococcia"
Thermales
Thermaceae

Allomeiothermus

Calidithermus

Meiothermus

Rhabdothermus

Vulcanithermus

Oceanithermus

Marinithermus

Thermus

Trueperales
Trueperaceae

Truepera

Deinococcales
Deinococcaceae

Deinobacterium

Deinococcus

"Deinococcia"
Deinococcales
"Marinithermaceae"

Marinithermus

Oceanithermus

Thermaceae

Allomeiothermus

Calidithermus

Meiothermus

Thermus

Trueperaceae

Truepera

Deinococcaceae

Deinobacterium

Deinococcus species-group 2

Deinococcus

Taxonomy

The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) [24] and National Center for Biotechnology Information (NCBI) [25]

Sequenced genomes

Currently there are 10 sequenced genomes of strains in this phylum. [26]

The two Meiothermus species were sequenced under the auspices of the Genomic Encyclopedia of Bacteria and Archaea project (GEBA), which aims at sequencing organisms based on phylogenetic novelty and not on pathogenicity or notoriety. [27]

See also

Related Research Articles

<span class="mw-page-title-main">Spirochaete</span> Phylum of bacteria

A spirochaete or spirochete is a member of the phylum Spirochaetota, which contains distinctive diderm (double-membrane) gram-negative bacteria, most of which have long, helically coiled cells. Spirochaetes are chemoheterotrophic in nature, with lengths between 3 and 500 μm and diameters around 0.09 to at least 3 μm.

The Aquificota phylum is a diverse collection of bacteria that live in harsh environmental settings. The name Aquificota was given to this phylum based on an early genus identified within this group, Aquifex, which is able to produce water by oxidizing hydrogen. They have been found in springs, pools, and oceans. They are autotrophs, and are the primary carbon fixers in their environments. These bacteria are Gram-negative, non-spore-forming rods. They are true bacteria as opposed to the other inhabitants of extreme environments, the Archaea.

The Chloroflexia are a class of bacteria in the phylum Chloroflexota. Chloroflexia are typically filamentous, and can move about through bacterial gliding. It is named after the order Chloroflexales.

The Thermomicrobia is a group of thermophilic green non-sulfur bacteria. Based on species Thermomicrobium roseum and Sphaerobacter thermophilus, this bacteria class has the following description:

<span class="mw-page-title-main">Chlamydiota</span> Phylum of bacteria

The Chlamydiota are a bacterial phylum and class whose members are remarkably diverse, including pathogens of humans and animals, symbionts of ubiquitous protozoa, and marine sediment forms not yet well understood. All of the Chlamydiota that humans have known about for many decades are obligate intracellular bacteria; in 2020 many additional Chlamydiota were discovered in ocean-floor environments, and it is not yet known whether they all have hosts. Historically it was believed that all Chlamydiota had a peptidoglycan-free cell wall, but studies in the 2010s demonstrated a detectable presence of peptidoglycan, as well as other important proteins.

<i>Thermus</i> Genus of bacteria

Thermus is a genus of thermophilic bacteria. It is one of several bacteria belonging to the Deinococcota phylum. Thermus species can be distinguished from other genera in the family Thermaceae as well as all other bacteria by the presence of eight conserved signature indels (CSIs) found in proteins such as adenylate kinase and replicative DNA helicase as well as 14 conserved signature proteins (CSPs) that are exclusively shared by members of this genus.

<span class="mw-page-title-main">Pasteurellaceae</span> Family of bacteria

The Pasteurellaceae comprise a large family of Gram-negative bacteria. Most members live as commensals on mucosal surfaces of birds and mammals, especially in the upper respiratory tract. Pasteurellaceae are typically rod-shaped, and are a notable group of facultative anaerobes. Their biochemical characteristics can be distinguished from the related Enterobacteriaceae by the presence of oxidase, and from most other similar bacteria by the absence of flagella.

The Thermotogota are a phylum of the domain Bacteria. The phylum contains a single class, Thermotogae. The phylum Thermotogota is composed of Gram-negative staining, anaerobic, and mostly thermophilic and hyperthermophilic bacteria.

The Synergistota is a phylum of anaerobic bacteria that show Gram-negative staining and have rod/vibrioid cell shape. Although Synergistota have a diderm cell envelope, the genes for various proteins involved in lipopolysaccharides biosynthesis have not yet been detected in Synergistota, indicating that they may have an atypical outer cell envelope. The Synergistota inhabit a majority of anaerobic environments including animal gastrointestinal tracts, soil, oil wells, and wastewater treatment plants and they are also present in sites of human diseases such as cysts, abscesses, and areas of periodontal disease. Due to their presence at illness related sites, the Synergistota are suggested to be opportunistic pathogens but they can also be found in healthy individuals in the microbiome of the umbilicus and in normal vaginal flora. Species within this phylum have also been implicated in periodontal disease, gastrointestinal infections and soft tissue infections. Other species from this phylum have been identified as significant contributors in the degradation of sludge for production of biogas in anaerobic digesters and are potential candidates for use in renewable energy production through their production of hydrogen gas. All of the known Synergistota species and genera are presently part of a single class (Synergistia), order (Synergistiales), and family (Synergistaceae).

The Chloroflexota are a phylum of bacteria containing isolates with a diversity of phenotypes, including members that are aerobic thermophiles, which use oxygen and grow well in high temperatures; anoxygenic phototrophs, which use light for photosynthesis ; and anaerobic halorespirers, which uses halogenated organics as electron acceptors.

<i>Deinococcus radiodurans</i> Radioresistant extremophile species of bacterium

Deinococcus radiodurans is a bacterium, an extremophile and one of the most radiation-resistant organisms known. It can survive cold, dehydration, vacuum, and acid, and therefore is known as a polyextremophile. It has been listed as the world's toughest known bacterium in The Guinness Book Of World Records.

<i>Deinococcus</i> Genus of bacteria

Deinococcus is in the monotypic family Deinococcaceae, and one genus of three in the order Deinococcales of the bacterial phylum Deinococcota highly resistant to environmental hazards. These bacteria have thick cell walls that give them Gram-positive stains, but they include a second membrane and so are closer in structure to Gram-negative bacteria. Deinococcus survive when their DNA is exposed to high doses of gamma and UV radiation. Whereas other bacteria change their structure in the presence of radiation, such as by forming endospores, Deinococcus tolerate it without changing their cellular form and do not retreat into a hardened structure. They are also characterized by the presence of the carotenoid pigment deinoxanthin that give them their pink color. They are usually isolated according to these two criteria. In August 2020, scientists reported that bacteria from Earth, particularly Deinococcus bacteria, were found to survive for three years in outer space, based on studies conducted on the International Space Station. These findings support the notion of panspermia, the hypothesis that life exists throughout the Universe, distributed in various ways, including space dust, meteoroids, asteroids, comets, planetoids or contaminated spacecraft.

The Negativicutes are a class of bacteria in the phylum Bacillota, whose members have a peculiar cell wall with a lipopolysaccharide outer membrane which stains gram-negative, unlike most other members of the Bacillota. Although several neighbouring Clostridia species also stain gram-negative, the proteins responsible for the unusual diderm structure of the Negativicutes may have actually been laterally acquired from Pseudomonadota. Additional research is required to confirm the origin of the diderm cell envelope in the Negativicutes.

Deinobacterium is a genus in the Deinococcota phylum (Bacteria). Not to be confused with Deinobacter, a disused name for Deinococcus.

There are several models of the Branching order of bacterial phyla, one of these was proposed in 1987 paper by Carl Woese.

Thermaceae is a family of bacteria belonging to the phylum Deinococcota. It is the only family in the order Thermales. They are particularly resistant to heat, and live in the benthic zone of the Gulf of Mexico.

<i>Meiothermus</i> Genus of bacteria

Meiothermus is a genus of Deinococcota bacteria. Members of Meiothermus can be reliably distinguished from other genera in the family Thermaceae as well as all other bacteria by the presence of three conserved signature indels (CSIs) found in the proteins: 5-methyltetrahydrofolate–homocysteine methyltransferase, cadmium transporter and polynucleotide phosphorylase and are exclusively shared by species of this genus. Meiothermus is also different than the Thermus genus, which it was originally a member of, in their optimum growth temperatures, with Meiothermus being able to grow in colder environments. Meiothermus was first isolated with Thermus in alkaline and neutral hot springs in Kamchatka, Russia and Yellowstone National Park, USA.

Deinococcus frigens is a species of low temperature and drought-tolerating, UV-resistant bacteria from Antarctica. It is Gram-positive, non-motile and coccoid-shaped. Its type strain is AA-692. Individual Deinococcus frigens range in size from 0.9-2.0 μm and colonies appear orange or pink in color. Liquid-grown cells viewed using phase-contrast light microscopy and transmission electron microscopy on agar-coated slides show that isolated D. frigens appear to produce buds. Comparison of the genomes of Deiococcus radiodurans and D. frigens have predicted that no flagellar assembly exists in D. frigens.

Deinococcus marmoris is a Gram-positive bacterium isolated from Antarctica. As a species of the genus Deinococcus, the bacterium is UV-tolerant and able to withstand low temperatures.

Coprothermobacterota is a phylum of nonmotile, rod-shaped bacteria.

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