| Club | League/Division | Lvl | Nickname | Change from 2023–24 |
|---|
| Saffron Dynamo | Midland League Division One | 10 | Saff | From United Counties League One |
| Saffron Walden Town | Essex Senior League | 9 | Bloods |
| Salford City | EFL League Two | 4 | Ammies |
| Salisbury | National League South | 6 | Whites | From Southern League Premier South |
| Saltash United | Western League Premier Division | 9 | Ashes |
| Saltdean United | Southern Combination League Premier Division | 9 | Tigers |
| Sandbach United | North West Counties League Division One South | 10 | Ramblers |
| Sandhurst Town | Combined Counties League Premier Division South | 9 | Fizzers |
| Sandiacre Town | United Counties League Division One | 10 | Saints |
| Sawbridgeworth Town | Essex Senior League | 9 | Robins | From Spartan South Midlands League Premier |
| Scarborough Athletic | National League North | 6 | Seadogs |
| Scunthorpe United | National League North | 6 | Iron |
| Seaford Town | Southern Combination League Division One | 10 | Badgers |
| Seaham Red Star | Northern League Division One | 9 | Star |
| Selby Town | Northern Counties East League Division One | 10 | Robins |
| Selsey | Southern Combination League Division One | 10 | Blues |
| Selston | United Counties League Division One | 10 | Parisioners |
| Sevenoaks Town | Isthmian League South East Division | 8 | Oaks |
| Shaftesbury | Southern League Division One South | 8 | Rockies | From Wessex League Premier |
| Shawbury United | North West Counties League Division One South | 10 | tbc | From Midland League One |
| Sheerwater | Combined Counties League Premier Division South | 9 | Sheers |
| Sheffield | Northern Premier League Division One East | 8 | Club |
| Sheffield United | EFL Championship | 2 | Blades | From Premier League |
| Sheffield Wednesday | EFL Championship | 2 | Owls |
| Shefford Town & Campton | Spartan South Midlands League Premier Division | 9 | tbc |
| Shelley | Northern Counties East League Division One | 10 | tbc | From North West Counties League One North |
| Sheppey Sports | Southern Counties East League Division One | 10 | Paperboys | Name change - formerly K Sports |
| Sheppey United | Isthmian League South Central Division | 8 | Ites |
| Shepshed Dynamo | Northern Premier League Division One Midlands | 8 | Dynamo |
| Shepton Mallet | Western League Premier Division | 9 | Mallet |
| Sherborne Town | Wessex League Premier Division | 9 | tbc |
| Sheringham | Eastern Counties League Premier Division | 9 | Shannocks |
| Sherwood Colliery | Northern Premier League Division One East | 8 | Parisioners | From United Counties League Premier North |
| Shifnal Town | Midland League Premier Division | 9 | Reds |
| Shildon | Northern League Division One | 9 | Railwaymen |
| Shirebrook Town | United Counties League Premier Division North | 9 | tbc | From Northern Counties East League One |
| Shirehampton | Western League Division One | 10 | tbc |
| Sholing | Southern League Premier Division South | 7 | Boatmen |
| Shoreham | Southern Combination League Premier Division | 9 | Musselmen |
| Shortwood United | Hellenic League Division One | 10 | Woods |
| Shrewsbury Town | EFL League One | 3 | Shrews |
| Sidmouth Town | South West Peninsula League Premier Division East | 10 | Vikings |
| Silsden | Northern Counties East League Premier Division | 9 | Cobbydalers |
| Sittingbourne | Isthmian League South East Division | 8 | Brickies |
| Skegness Town | United Counties League Premier Division North | 9 | Lilywhites |
| Skelmersdale United | North West Counties League Division One North | 10 | Skem | From North West Counties League Premier |
| Sleaford Town | United Counties League Premier Division North | 9 | Greens |
| Slimbridge | Hellenic League Premier Division | 9 | Swans |
| Slough Town | National League South | 6 | Rebels |
| Smethwick Rangers | Midland League Division One | 10 | SR1 |
| Snodland Town | Southern Counties East League Premier Division | 9 | tbc |
| Soham Town Rangers | Eastern Counties League Premier Division | 9 | Greens |
| Solihull Moors | National League | 5 | Moors |
| Soul Tower Hamlets | Eastern Counties League Division One South | 10 | Green Army | From Eastern Counties League One South (also new name - formerly Tower Hamlets) |
| South Leeds | Northern Counties East League Division One | 10 | tbc | From Yorkshire Amateur League Supreme (also new name - formerly Middleton). |
| South Liverpool | North West Counties League Premier Division | 9 | South | From North West Counties League One North |
| South Park | Isthmian League South Central Division | 8 | Sparks |
| South Shields | National League North | 6 | Mariners |
| Southall | Isthmian League South Central Division | 8 | tbc |
| Southam United | Hellenic League Division One | 10 | Saints |
| Southampton | Premier League | 1 | Saints | From EFL Championship |
| Southend Manor | Eastern Counties League Division One South | 10 | Manor |
| Southend United | National League | 5 | Shrimpers |
| Southport | National League North | 6 | Sandgrounders |
| Southwell City | United Counties League Division One | 10 | Bramleys |
| Spalding United | Southern League Premier Division Central | 7 | Tulips | From Northern Premier League One Midlands |
| Spartans Youth | Combined Counties League Division One | 10 | tbc |
| Spelthorne Sports | Combined Counties League Premier Division South | 9 | Spelly |
| Spennymoor Town | National League North | 6 | Moors |
| Sporting Bengal United | Isthmian League Division One North | 8 | Bengal Tigers | From Essex Senior League |
| Sporting Club Inkberrow | Hellenic League Premier Division | 9 | Eagles | From Hellenic League One |
| Sporting Club Thamesmead | Southern Counties East League Division One | 10 | Acres |
| Sporting Khalsa | Northern Premier League Division One Midlands | 8 | Sporting |
| Squires Gate | North West Counties League Premier Division | 9 | Gate |
| St Albans City | National League South | 6 | Saints |
| St. Blazey | Western League Premier Division | 9 | Green & Blacks |
| St Day | South West Peninsula League Premier Division West | 10 | tbc | From St. Piran League West |
| St Ives Town | Southern League Premier Division Central | 7 | Saints |
| St Neots Town | United Counties League Premier Division South | 9 | Saints |
| St Margaretsbury | Eastern Counties League Division One South | 10 | Bury |
| Stafford Rangers | Northern Premier League Division One West | 8 | Rangers | From Northern Premier League Premier |
| Stafford Town | North West Counties League Division One South | 10 | Reds |
| Staines & Lammas (Middlesex) | Combined Counties League Division One | 10 | Lammas | From Surrey Premier County League |
| Stalybridge Celtic | Northern Premier League Division One West | 8 | Celts |
| Stamford | Northern Premier League Premier Division | 7 | Daniels |
| Stansfeld O&BC | Southern Counties East League Premier Division | 9 | Palace |
| Stansted | Essex Senior League | 9 | Blues | From Spartan South Midlands League Premier |
| Stanway Pegasus | Eastern Counties League Division One North | 10 | tbc |
| Stanway Rovers | Essex Senior League | 9 | Rovers |
| Stapenhill | Midland League Division One | 10 | Swans |
| Stapleford Town | United Counties League Division One | 10 | tbc | From Nottinghamshire Senior League Premier |
| Staplehurst Monarchs | Southern Counties East League Division One | 10 | Monarchs |
| Staveley Miners Welfare | United Counties League Division One | 10 | Welfare | From Northern Counties East League One |
| Steeton | North West Counties League Division One North | 10 | tbc |
| Stevenage | EFL League One | 3 | Boro |
| Steyning Town | Isthmian League South East Division | 8 | Barrowmen | From Southern Combination League Premier |
| Sticker | South West Peninsula League Premier Division West | 10 | Ashes |
| Stockport County | EFL League One | 3 | Hatters | From EFL League Two |
| Stockport Georgians | North West Counties League Division One South | 10 | Georgians |
| Stockport Town | North West Counties League Premier Division | 9 | Lions | From North West Counties League One South |
| Stocksbridge Park Steels | Northern Premier League Division One East | 8 | Steels |
| Stockton Town | Northern Premier League Premier Division | 7 | Anchors | From Northern Premier League One East |
| Stoke City | EFL Championship | 2 | Potters |
| Stoke Gabriel & Torbay Police | South West Peninsula League Premier Division East | 10 | Bees |
| Stone Old Alleynians | Midland League Premier Division | 9 | tbc |
| Stonehouse Town | Hellenic League Division One | 10 | Magpies |
| Storrington | Southern Combination League Division One | 10 | Swans | From Southern Combination League Two |
| Stotfold | Southern League Division One Central | 8 | Eagles |
| Stotfold Reserves | Spartan South Midlands League Division One | 10 | Eagles | Joined the League |
| Stourbridge | Southern League Premier Division Central | 7 | Glassboys |
| Stourport Swifts | Midland League Premier Division | 9 | Swifts |
| Stowmarket Town | Eastern Counties League Premier Division | 9 | Old Black & Golds | From Isthmian League One North |
| Stratford Town | Southern League Premier Division Central | 7 | Town |
| Street | Western League Premier Division | 9 | Cobblers |
| Studley | Midland League Premier Division | 9 | Bees |
| Sturminster Newton United | Western League Division One | 10 | Cherries | From Dorset Premier League |
| Sunderland | EFL Championship | 2 | Black Cats |
| Sunderland Ryhope CA | Northern League Division Two | 10 | CA | From Northern League One |
| Sunderland West End | Northern League Division Two | 10 | Albion |
| Sutton Athletic | Southern Counties East League Premier Division | 9 | tbc |
| Sutton Coldfield Town | Northern Premier League Division One Midlands | 8 | Royals |
| Sutton Common Rovers | Isthmian League South Central Division | 8 | Commoners |
| Sutton United (Birmingham) | Midland League Division One | 10 | tbc |
| Sutton United | National League | 5 | U's | From EFL League Two |
| Swaffham Town | Eastern Counties League Division One North | 10 | Pedlars |
| Swallownest | Northern Counties East League Division One | 10 | tbc |
| Swansea City (WE) | EFL Championship | 2 | Swans |
| Swindon Supermarine | Southern League Premier Division South | 7 | Marine |
| Swindon Town | EFL League Two | 4 | Robins |
|
