Walter Gilbert

Last updated
Walter Gilbert
ForMemRS
Walter Gilbert HD2008 portrait.JPG
Walter Gilbert in 2008
Born (1932-03-21) March 21, 1932 (age 92)
Boston, Massachusetts, U.S.
Education
Known forDNA sequencing
Spouse
Celia Stone
​
(m. 1953)​
[1]
Children2 [1]
Awards
Scientific career
Fields
Institutions Harvard University
Thesis On generalised dispersion relations and meson-nucleon scattering  (1958)
Doctoral advisor Abdus Salam
Doctoral students
Website www.nobelprize.org/nobel_prizes/chemistry/laureates/1980/gilbert-bio.html

Walter Gilbert (born March 21, 1932) is an American biochemist, physicist, molecular biology pioneer, and Nobel laureate. [1] [4] [5]

Contents

Education and early life

Walter Gilbert was born in Boston, Massachusetts, on March 21, 1932, into a Jewish family, [6] the son of Emma (Cohen), a child psychologist, and Richard V. Gilbert, an economist. [4] [7]

When Gilbert was seven years old, the family moved to the Washington D.C. area so his father could work under Harry Hopkins on the New Deal brain trust. While living in Washington the family became friends with the family of I.F. Stone and Wally met Stone's oldest daughter, Celia, when they were both 8. They later married at age 21. [8]

He was educated at the Sidwell Friends School, and attended Harvard University for undergraduate and graduate studies, earning a baccalaureate in chemistry and physics in 1953 and a master's degree in physics in 1954. [4] He studied for his doctorate at the University of Cambridge, where he earned a PhD in physics supervised by the Nobel laureate Abdus Salam in 1957. [4] [9]

Career and research

Gilbert returned to Harvard in 1956 and was appointed assistant professor of physics in 1959. [4] Gilbert's wife Celia worked for James Watson, leading Gilbert to become interested in molecular biology. Watson and Gilbert ran their laboratory jointly through most of the 1960s, until Watson left for Cold Spring Harbor Laboratory. [10] In 1964 he was promoted to associate professor of biophysics and promoted again in 1968 to professor of biochemistry. [4]

Gilbert is a co-founder of the biotech start-up companies Biogen, with Kenneth Murray, Phillip Sharp and Charles Weissman [11] and Myriad Genetics with Dr. Mark Skolnick and Kevin Kimberlin [12] [13] where he was the first chairman on their respective boards of directors. Gilbert left his position at Harvard to run Biogen as CEO, but was later asked to resign by the company's board of directors. [14] He is a member of the Board of Scientific Governors at The Scripps Research Institute. Gilbert has served as the chairman of the Harvard Society of Fellows.

In 1996, Gilbert and Stuart B. Levy founded Paratek Pharmaceuticals. Gilbert served as chairman until 2014. [15]

Gilbert was an early proponent of sequencing the human genome. At a March 1986 meeting in Santa Fe New Mexico he proclaimed "The total human sequence is the grail of human genetics". In 1987, he proposed starting a company called Genome Corporation to sequence the genome and sell access to the information. [14] In an opinion piece in Nature in 1991, he envisioned completion of the human genome sequence transforming biology into a field in which computer databases would be as essential as laboratory reagents [16]

Gilbert returned to Harvard in 1985. [17] Gilbert was an outspoken critic of David Baltimore in the handling of the scientific fraud accusations against Thereza Imanishi-Kari. [18] Gilbert also joined the early controversy over the cause of AIDS. [19] In 1962, Gilbert's physics PhD student Gerald Guralnik extended Gilbert's work on massless particles; Guralnik's work is widely recognized as an important thread in the discovery of the Higgs Boson. [20]

With his PhD student Benno Müller-Hill, Gilbert was the first to purify the lac repressor, [21] just beating out Mark Ptashne for purifying the first gene regulatory protein. [22]

Together with Allan Maxam, Gilbert developed a new DNA sequencing method, Maxam–Gilbert sequencing, [23] [24] using chemical methods developed by Andrei Mirzabekov. His approach to the first synthesis of insulin via recombinant DNA [25] lost out to Genentech's approach which used genes built up from the nucleotides rather than from natural sources. Gilbert's effort was hampered by a temporary moratorium on recombinant DNA work in Cambridge, Massachusetts, forcing his group to move their work to an English biological weapons site. [26]

Gilbert first proposed the terms introns (intragenic regions) and exons (expressed regions) in reference to recently discovered phenomenon of splicing [27] and suggested explanation for the evolution of introns in a seminal 1978 "News and Views" correspondence to Nature titled "why genes in pieces?". [28] In 1986, Gilbert proposed the RNA world hypothesis for the origin of life, [29] based on a concept first proposed by Carl Woese in 1967.

Awards and honors

Walter Gilbert portrait via the National Library of Medicine WalterGilbert2.jpg
Walter Gilbert portrait via the National Library of Medicine

In 1969, Gilbert was awarded Harvard's Ledlie Prize. [4] In 1972 he was named American Cancer Society Professor of Molecular Biology. [4] In 1979, Gilbert was awarded the Louisa Gross Horwitz Prize from Columbia University together with Frederick Sanger. [4] [30] That year he was also awarded the Gairdner Prize and the Albert Lasker Award for Basic Medical Research. [4]

Gilbert was awarded the 1980 Nobel Prize in Chemistry, shared with Frederick Sanger and Paul Berg. Gilbert and Sanger were recognized for their pioneering work in devising methods for determining the sequence of nucleotides in a nucleic acid.

Gilbert has also been honored by the National Academy of Sciences (US Steel Foundation Award, 1968); Massachusetts General Hospital (Warren Triennial Prize, 1977); [31] the New York Academy of Sciences; (Louis and Bert Freedman Foundation Award, 1977), the Académie des Sciences of France (Prix Charles-Leopold Mayer Award, 1977). [4] Gilbert was elected a Foreign Member of the Royal Society (ForMemRS) in 1987. [4] [32] [33]

In 2002, he received the Biotechnology Heritage Award, from the Biotechnology Industry Organization (BIO) and the Chemical Heritage Foundation. [34] [35]

Allan Maxam and Walter Gilbert's 1977 paper "A new method for sequencing DNA" was honored by a Citation for Chemical Breakthrough Award from the Division of History of Chemistry of the American Chemical Society for 2017. It was presented to the Department of Molecular & Cellular Biology, Harvard University. [36] [24]

Personal life

Gilbert married Celia Stone, the daughter of I. F. Stone, in 1953 and has two children. [1] After retiring from Harvard in 2001, Gilbert has launched an artistic career to combine art and science. His art format is centered on digital photography. [17] [37]

Purple Swirl by Wally Gilbert Purple Swirl by Wally Gilbert.jpg
Purple Swirl by Wally Gilbert

See also

Related Research Articles

An intron is any nucleotide sequence within a gene that is not expressed or operative in the final RNA product. The word intron is derived from the term intragenic region, i.e., a region inside a gene. The term intron refers to both the DNA sequence within a gene and the corresponding RNA sequence in RNA transcripts. The non-intron sequences that become joined by this RNA processing to form the mature RNA are called exons.

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<span class="mw-page-title-main">DNA sequencing</span> Process of determining the nucleic acid sequence

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References

  1. 1 2 3 4 Walter Gilbert on Nobelprize.org OOjs UI icon edit-ltr-progressive.svg , accessed 11 October 2020
  2. ↑ Church, G.; Gilbert, W. (1984). "Genomic sequencing". Proceedings of the National Academy of Sciences of the United States of America. 81 (7): 1991–1995. Bibcode:1984PNAS...81.1991C. doi: 10.1073/pnas.81.7.1991 . PMC   345422 . PMID   6326095.
  3. ↑ "Jack Greenblatt". academictree.org.
  4. 1 2 3 4 5 6 7 8 9 10 11 12 Shampo MA, Kyle RA (May 2003). "Walter Gilbert--1980 Nobel Prize for Chemistry". Mayo Clin. Proc. 78 (5): 588. doi: 10.4065/78.5.588 . PMID   12744546.
  5. ↑ Walter Gilbert's publications indexed by the Scopus bibliographic database. (subscription required)
  6. ↑ JINFO. "Jewish Nobel Prize Winners in Chemistry". www.jinfo.org. Retrieved 2023-03-30.
  7. ↑ "Home - the Chicago Literary Club" (PDF).
  8. ↑ Saltzman, Jonathan (2018-03-31). "Five things you should know about Wally Gilbert". The Boston Globe . Retrieved 2018-04-01.
  9. ↑ Gilbert, Walter (1958). On generalised dispersion relations and meson-nucleon scattering (PhD thesis). University of Cambridge. OCLC   879396761.
  10. ↑ Watson, James D. (2003). Genes, Girls and Gamow.
  11. ↑ Stendahl, Max (May 31, 2018). "40 years later, Biogen's founders Get the Band Back Together". Boston Business Journal.
  12. ↑ Davies, Kevin; White, Michael (1996). Breakthrough: The Race to Find the Breast Cancer Gene. Wiley. p. 199.
  13. ↑ "MYRIAD: PIONEERING PREDICTIVE MEDICINE". University of Utah. April 25, 2018.
  14. 1 2 Kanigel, Robert (December 13, 1987). "The Genome Project". The New York Times Magazine: 44, 98–101, 106. PMID   11658922.
  15. ↑ "Founders".
  16. ↑ Gilbert, Walter (1991). "Towards a paradigm shift in biology". Nature. 349 (6305): 99. Bibcode:1991Natur.349...99G. doi: 10.1038/349099a0 . PMID   1986314. S2CID   1173431.
  17. 1 2 Johnson, Carolyn Y. (March 13, 2015). "A physicist, biologist, Nobel laureate, CEO, and now, artist". The Boston Globe.
  18. ↑ Kolata, Gina (June 25, 1996). "Inquiry lacking due process". The New York Times Books.
  19. ↑ Cohen, Jon (December 9, 1984). "The Duesberg Phenomenon" (PDF). Science. 266 (5191): 1643–1644. Bibcode:1994Sci...266.1642C. doi:10.1126/science.7992043. PMID   7992043.
  20. ↑ Close, Frank (2013). The Infinity Puzzle: The Personalities, Politics and Extraordinary Science Behind the Higgs Boson. Oxford University Press. pp. 146–147.
  21. ↑ Gilbert, Walter; Müller-Hill, Benno (December 1966). "Isolation of the lac repressor". PNAS. 56 (6): 1891–1898. Bibcode:1966PNAS...56.1891G. doi: 10.1073/pnas.56.6.1891 . PMC   220206 . PMID   16591435.
  22. ↑ Ptashne, Mark (February 1967). "Isolation of the lambda phage repressor". PNAS. 57 (2): 306–313. Bibcode:1967PNAS...57..306P. doi: 10.1073/pnas.57.2.306 . PMC   335506 . PMID   16591470.
  23. ↑ Maxam, Allan; Gilbert, Walter (1980). "Sequencing end-labeled DNA with base-specific chemical cleavages". Nucleic Acids Part I. Methods in Enzymology. Vol. 65. pp. 499–560. doi:10.1016/S0076-6879(80)65059-9. ISBN   9780121819651. PMID   6246368.
  24. 1 2 Maxam, A.; Gilbert, W. (1977). "A new method for sequencing DNA". Proceedings of the National Academy of Sciences of the United States of America. 74 (2): 560–564. Bibcode:1977PNAS...74..560M. doi: 10.1073/pnas.74.2.560 . PMC   392330 . PMID   265521.
  25. ↑ Villa-Komaroff L, Efstratiadis A, Broome S, Lomedico P, Tizard R, Naber SP, Chick WL, Gilbert W (1978). "A bacterial clone synthesizing proinsulin". PNAS. 75 (8): 3727–31. Bibcode:1978PNAS...75.3727V. doi: 10.1073/pnas.75.8.3727 . PMC   392859 . PMID   358198.
  26. ↑ Hall, Stephen S. (1987). Invisible Frontiers: The Race to Synthesize a Human Gene. Atlantic Monthly Press.
  27. ↑ Berget SM, Moore C, Sharp PA (August 1977). "Spliced segments at the 5' terminus of adenovirus 2 late mRNA". Proceedings of the National Academy of Sciences of the United States of America. 74 (8): 3171–3175. Bibcode:1977PNAS...74.3171B. doi: 10.1073/pnas.74.8.3171 . PMC   431482 . PMID   269380.
  28. ↑ Gilbert, Walter (February 9, 1978). "Why genes in pieces". Nature. 271 (5645): 501. Bibcode:1978Natur.271..501G. doi: 10.1038/271501a0 . PMID   622185. S2CID   4216649.
  29. ↑ Gilbert, W. (1986). "Origin of life: The RNA world". Nature. 319 (6055): 618. Bibcode:1986Natur.319..618G. doi: 10.1038/319618a0 . S2CID   8026658.
  30. ↑ Brownlee, George G. (2015). "Frederick Sanger CBE CH OM. 13 August 1918 — 19 November 2013". Biographical Memoirs of Fellows of the Royal Society . 61. Royal Society publishing: 437–466. doi: 10.1098/rsbm.2015.0013 . ISSN   0080-4606.
  31. ↑ "Warren Triennial Prize". ecor.mgh.harvard.edu. Archived from the original on November 27, 2020. Retrieved 2021-10-05.
  32. ↑ "Professor Walter Gilbert ForMemRS". London: Royal Society. Archived from the original on 2015-09-22.
  33. ↑ "Fellowship of the Royal Society 1660-2015". Royal Society. Archived from the original on 2015-10-15.
  34. ↑ "Biotechnology Heritage Award". Science History Institute. 2016-05-31. Retrieved 22 March 2018.
  35. ↑ "Paratek Pharmaceuticals Chairman and Co-Founder Dr. Walter Gilbert Receives Heritage Award at BIO 2002". PR NewsWire. 10 June 2002. Retrieved 5 February 2014.
  36. ↑ "Citations for Chemical Breakthrough Awards 2017 Awardees". Division of the History of Chemistry. Retrieved 12 March 2018.
  37. ↑ "Wally Gilbert "Worlds"". www.nyartbeat.com. Retrieved 2019-12-16.
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