Ensifer numidicus

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Ensifer numidicus
Rhizobia nodules on Vigna unguiculata.jpg
Rhizobia nodules attached to roots of Vigna unguiculata
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Bacteria
Phylum: Pseudomonadota
Class: Alphaproteobacteria
Order: Hyphomicrobiales
Family: Rhizobiaceae
Genus: Ensifer
Species:
E. numidicus
Binomial name
Ensifer numidicus
Merabet et al. 2010
Type strain
ORS 1444, ORS1410, ORS 1407, LMG 24690,CIP 109850, PN14, LBi2

Ensifer numidicus is a nitrogen fixing symbiont of Fabaceae. [1] gram-negative, aerobic, non-spore forming, rod-shaped bacterium of the family Rhizobiaceae. [2] First described in 2010; [1] more biovars have since been isolated and described [3] with ORS 1407 considered the representative organism. [4] Most examples have been found in arid and infra-arid regions of Tunisia. [5]

Host plants

Biovars has been shown to induce nodule formation in a wide variety of symbiosis competent plant species including Medicago Sativa(cultivated alfalfa) [6] , Lotus creticus [3] ,Syrian mesquite(Prosopis farcta), [7] Lens culinaris Medikus ssp(lentils) [3] [8] as well as Cicer arietinum (chickpea) [3] and Argyrolobium uniflorum. [1]

Associated Biovars

Argyrolobium uniflorum: ORS 1407 [1]

cultivated alfalfa (Medicago sativa) :ORS 1407 [1]

Lotus creticus: PT26 [7] , ORS 1410 [3]

Cultivated lentils(Lens culinaris): ORS 1444 [3]

Cicer arietinum (chickpea):LBi2 [3]

Syrian mesquite(Prosopis farcta):PN14 [7]

Known relationships between cultivars

numidicus

This phylogeny is based on a constrained analysis of the 16S ribosomal RNA [1]

Genome

16s RNA analysis has found Ensifer numidicus to be closely related to Ensifer medicae and Ensifer garamanticus. [3] Analogous genes between closely related species suggests high levels of horizontal gene transfer between closely related species. [3] Laboratory inoculation has shown Ensifer numidicus engages in indeterminate nodulation with host plants in at least some circumstances. [9]

Growth conditions

E. numidicus has been found to grow on yeast-mannitol medium at 28C with an upper limit of 40C. [1] Laboratory cultivated strains have found metabolism of at least 13 substrates including dulcitol, D-lyxose, 1-O-methyl a-D-glucopyranoside, 3-O-methyl-D-glucopyranose, D-gluconate, L-histidine, succinate, fumarate, ethanolamine, DL-b-hydroxybutyrate, L-aspartate, L-alanine and propionate. [1] Sensitivity has been found to salt concentrations greater than 4%. [3] Due to similarities to other Ensifer species, it cannot be described by growth conditions alone and must be differentiated by genetic components. [3]

Related Research Articles

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Botany, also called plant science, plant biology or phytology, is the science of plant life and a branch of biology. A botanist, plant scientist or phytologist is a scientist who specialises in this field. The term "botany" comes from the Ancient Greek word βοτάνη meaning "pasture", "herbs" "grass", or "fodder"; βοτάνη is in turn derived from βόσκειν, "to feed" or "to graze". Traditionally, botany has also included the study of fungi and algae by mycologists and phycologists respectively, with the study of these three groups of organisms remaining within the sphere of interest of the International Botanical Congress. Nowadays, botanists study approximately 410,000 species of land plants of which some 391,000 species are vascular plants, and approximately 20,000 are bryophytes.

<span class="mw-page-title-main">Endosymbiont</span> Organism that lives within the body or cells of another organism

An endosymbiont or endobiont is any organism that lives within the body or cells of another organism most often, though not always, in a mutualistic relationship. This phenomenon is known as endosymbiosis. Examples are nitrogen-fixing bacteria, which live in the root nodules of legumes, single-cell algae inside reef-building corals and bacterial endosymbionts that provide essential nutrients to insects.

<span class="mw-page-title-main">Soil</span> Mixture of organic matter, minerals, gases, liquids, and organisms that together support life

Soil, also commonly referred to as earth or dirt, is a mixture of organic matter, minerals, gases, liquids, and organisms that together support the life of plants and soil organisms. Some scientific definitions distinguish dirt from soil by restricting the former term specifically to displaced soil.

<span class="mw-page-title-main">Chickpea</span> Species of flowering plant with edible seeds in the family Fabaceae

The chickpea or chick pea is an annual legume of the family Fabaceae, subfamily Faboideae. Its different types are variously known as gram or Bengal gram, chhana, chana, or channa, garbanzo or garbanzo bean, or Egyptian pea. Chickpea seeds are high in protein. It is one of the earliest cultivated legumes, the oldest archaeological evidence of which was found in Syria.

<span class="mw-page-title-main">Fabaceae</span> Family of legume flowering plants

The Fabaceae or Leguminosae, commonly known as the legume, pea, or bean family, are a large and agriculturally important family of flowering plants. It includes trees, shrubs, and perennial or annual herbaceous plants, which are easily recognized by their fruit (legume) and their compound, stipulate leaves. The family is widely distributed, and is the third-largest land plant family in number of species, behind only the Orchidaceae and Asteraceae, with about 765 genera and nearly 20,000 known species.

<span class="mw-page-title-main">Alfalfa</span> Perennial flowering plant

Alfalfa, also called lucerne, is a perennial flowering plant in the legume family Fabaceae. It is cultivated as an important forage crop in many countries around the world. It is used for grazing, hay, and silage, as well as a green manure and cover crop. The name alfalfa is used in North America. The name lucerne is more commonly used in the United Kingdom, South Africa, Australia, and New Zealand. The plant superficially resembles clover, especially while young, when trifoliate leaves comprising round leaflets predominate. Later in maturity, leaflets are elongated. It has clusters of small purple flowers followed by fruits spiralled in two to three turns containing 10–20 seeds. Alfalfa is native to warmer temperate climates. It has been cultivated as livestock fodder since at least the era of the ancient Greeks and Romans.

<i>Ensifer meliloti</i> Species of bacterium

Ensifer meliloti are an aerobic, Gram-negative, and diazotrophic species of bacteria. S. meliloti are motile and possess a cluster of peritrichous flagella. S. meliloti fix atmospheric nitrogen into ammonia for their legume hosts, such as alfalfa. S. meliloti forms a symbiotic relationship with legumes from the genera Medicago, Melilotus and Trigonella, including the model legume Medicago truncatula. This symbiosis promotes the development of a plant organ, termed a root nodule. Because soil often contains a limited amount of nitrogen for plant use, the symbiotic relationship between S. meliloti and their legume hosts has agricultural applications. These techniques reduce the need for inorganic nitrogenous fertilizers.

<span class="mw-page-title-main">Nod factor</span> Signaling molecule

Nod factors, are signaling molecules produced by soil bacteria known as rhizobia in response to flavonoid exudation from plants under nitrogen limited conditions. Nod factors initiate the establishment of a symbiotic relationship between legumes and rhizobia by inducing nodulation. Nod factors produce the differentiation of plant tissue in root hairs into nodules where the bacteria reside and are able to fix nitrogen from the atmosphere for the plant in exchange for photosynthates and the appropriate environment for nitrogen fixation. One of the most important features provided by the plant in this symbiosis is the production of leghemoglobin, which maintains the oxygen concentration low and prevents the inhibition of nitrogenase activity.

<i>Ensifer</i> (bacterium) Genus of bacteria

Ensifer is a genus of nitrogen-fixing bacteria (rhizobia), three of which have been sequenced.

<span class="mw-page-title-main">Crop wild relative</span> Wild plant closely related to a domesticated plant

A crop wild relative (CWR) is a wild plant closely related to a domesticated plant. It may be a wild ancestor of the domesticated (cultivated) plant or another closely related taxon.

<span class="mw-page-title-main">Water on Mars</span> Study of past and present water on Mars

Almost all water on Mars today exists as ice, though it also exists in small quantities as vapor in the atmosphere. What was thought to be low-volume liquid brines in shallow Martian soil, also called recurrent slope lineae, may be grains of flowing sand and dust slipping downhill to make dark streaks. While most water ice is buried, it is exposed at the surface across several locations on Mars. In the mid-latitudes, it is exposed by impact craters, steep scarps and gullies. Additionally, water ice is also visible at the surface at the north polar ice cap. Abundant water ice is also present beneath the permanent carbon dioxide ice cap at the Martian south pole. More than 5 million km3 of ice have been detected at or near the surface of Mars, enough to cover the whole planet to a depth of 35 meters (115 ft). Even more ice might be locked away in the deep subsurface. Some liquid water may occur transiently on the Martian surface today, but limited to traces of dissolved moisture from the atmosphere and thin films, which are challenging environments for known life. No evidence of present-day liquid water has been discovered on the planet's surface because under typical Martian conditions, warming water ice on the Martian surface would sublime at rates of up to 4 meters per year. Before about 3.8 billion years ago, Mars may have had a denser atmosphere and higher surface temperatures, potentially allowing greater amounts of liquid water on the surface, possibly including a large ocean that may have covered one-third of the planet. Water has also apparently flowed across the surface for short periods at various intervals more recently in Mars' history. Aeolis Palus in Gale Crater, explored by the Curiosity rover, is the geological remains of an ancient freshwater lake that could have been a hospitable environment for microbial life. The present-day inventory of water on Mars can be estimated from spacecraft images, remote sensing techniques, and surface investigations from landers and rovers. Geologic evidence of past water includes enormous outflow channels carved by floods, ancient river valley networks, deltas, and lakebeds; and the detection of rocks and minerals on the surface that could only have formed in liquid water. Numerous geomorphic features suggest the presence of ground ice (permafrost) and the movement of ice in glaciers, both in the recent past and present. Gullies and slope lineae along cliffs and crater walls suggest that flowing water continues to shape the surface of Mars, although to a far lesser degree than in the ancient past.

The Legume Information System (LIS) is legume sciences portal specifically for legume breeders and researchers, established and supported by the Agricultural Research Service of the United States Department of Agriculture. The mission of the Legume Information System is "to facilitate discoveries and crop improvement in the legumes," in particular to improve crop yields, their nutritional value, and our understanding of basic legume science.

<i>Trigonella suavissima</i> Species of plant

Trigonella suavissima is a herbaceous plant that is endemic to Australia. It is a member of the genus Trigonella and the family Fabaceae. Common names include Cooper clover, Menindee clover, calomba, Darling trigonella, sweet fenugreek, channel clover, sweet-scented clover and Australian shamrock.

Mesorhizobium mediterraneum is a bacterium from the genus Mesorhizobium, which was isolated from root nodule of the Chickpea in Spain. The species Rhizobium mediterraneum was subsequently transferred to Mesorhizobium mediterraneum. This species, along with many other closely related taxa, have been found to promote production of chickpea and other crops worldwide by forming symbiotic relationships.

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<i>Lotus creticus</i> Species of herb

Lotus creticus is a species of perennial herb of the family Fabaceae found in tropical Africa. It is symbiosis-competent and engages in nitrogen-fixing symbiotic interactions with species of the Ensifer genus[3][3]. It comprises three varieties found in the Mediterranean. There is some controversy as to whether each subgroup could be considered the same species but are classically described as being subgroups. Varieties consist of the most commonly cited silky-hairy var. creticus, which is widely distributed in its western part of the coast; the non-silky var. glabrescens, which has a western Mediterranean distribution; and the eastern Mediterranean var. collinus, which is also not silky and can be described by long petioles and peduncles.

Argyrolobium uniflorum is a wild pluriannual herbaceous species of drought tolerant legume found in arid regions of Northern Africa sometimes described as pseudo-savannah. A. uniflorum is a hardy wild species of plant and it is considered a valuable forage crop for agriculture in Africa. It is also useful as a potentially important species in bioremediation and dryland restoration in arid regions of Tusinia which are sensitive to misappropriation and overgrazing.

References

  1. 1 2 3 4 5 6 7 8 Merabet, C.; Martens, M.; Mahdhi, M.; Zakhia, F.; Sy, A.; Le Roux, C.; Domergue, O.; Coopman, R.; Bekki, A.; Mars, M.; Willems, A. (March 2010). "Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov". International Journal of Systematic and Evolutionary Microbiology. 60 (Pt 3): 664–674. doi:10.1099/ijs.0.012088-0. hdl: 1854/LU-936490 . ISSN   1466-5026. PMID   19656922.
  2. Podstawka, Adam. "Ensifer numidicus | Type strain | LMG 24690, CIP 109850, ORS 1407 | BacDiveID:133535". bacdive.dsmz.de. Retrieved 2021-09-02.
  3. 1 2 3 4 5 6 7 8 9 10 11 Sami, Dhaoui; Mokhtar, Rejili; Peter, Mergaert; Mohamed, Mars (2016). "Rhizobium leguminosarum symbiovar trifolii, Ensifer numidicus and Mesorhizobium amorphae symbiovar ciceri (or Mesorhizobium loti) are new endosymbiotic bacteria of Lens culinaris Medik". FEMS Microbiology Ecology. 92 (8): undefined. doi: 10.1093/femsec/fiw118 . PMID   27267929.
  4. Parker, Charles Thomas; Taylor, Dorothea; Garrity, George M. (2010). Parker, Charles Thomas; Garrity, George M (eds.). "Strain ORS 1407 (=LMG 24690 =CIP 109850) : Microbial Characteristics and Genomic Information". doi:10.1601/ex.17831 (inactive 2024-04-17).{{cite journal}}: Cite journal requires |journal= (help)CS1 maint: DOI inactive as of April 2024 (link)
  5. Bessadok, Khouloud; Navarro-Torre, Salvadora; Fterich, Amira; Caviedes, Miguel Angel; Pajuelo, Eloisa; Rodríguez-Llorente, Ignacio David; Mars, Mohamed (2021-05-01). "Diversity of rhizobia isolated from Tunisian arid soils capable of forming nitrogen-fixing symbiosis with Anthyllis henoniana". Journal of Arid Environments. 188: 104467. Bibcode:2021JArEn.188j4467B. doi:10.1016/j.jaridenv.2021.104467. ISSN   0140-1963. S2CID   233567533.
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  7. 1 2 3 Fterich, A.; Mahdhi, M.; Caviedes, M. A.; Pajuelo, E.; Rivas, R.; Rodriguez-Llorente, I. D.; Mars, M. (2011-06-01). "Characterization of root-nodulating bacteria associated to Prosopis farcta growing in the arid regions of Tunisia". Archives of Microbiology. 193 (6): 385–397. doi:10.1007/s00203-011-0683-z. ISSN   1432-072X. PMID   21359955. S2CID   24398589.
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