GGDEF domain

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GGDEF domain
PDB 1w25 EBI.jpg
response regulator PleD in complex with c-diGMP
Identifiers
SymbolGGDEF
Pfam PF00990
Pfam clan CL0276
InterPro IPR000160
SCOP2 1w25 / SCOPe / SUPFAM
CDD cd01949
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary

In molecular biology, a GGDEF domain is a protein domain which appears to be ubiquitous in bacteria and is often linked to a regulatory domain, such as a phosphorylation receiver or oxygen sensing domain. Its function is to act as a diguanylate cyclase and synthesize cyclic di-GMP, which is used as an intracellular signalling molecule in a wide variety of bacteria. [1] [2] Enzymatic activity can be strongly influenced by the adjacent domains. Processes regulated by this domain include exopolysaccharide synthesis, biofilm formation, motility and cell differentiation.

Structural studies of PleD from Caulobacter crescentus show that this domain forms a five-stranded beta sheet surrounded by helices, similar to the catalytic core of adenylate cyclase. [3]

References

  1. Paul R, Weiser S, Amiot NC, Chan C, Schirmer T, Giese B, Jenal U (March 2004). "Cell cycle-dependent dynamic localization of a bacterial response regulator with a novel di-guanylate cyclase output domain". Genes Dev. 18 (6): 715–27. doi:10.1101/gad.289504. PMC   387245 . PMID   15075296.
  2. Ryjenkov DA, Tarutina M, Moskvin OV, Gomelsky M (March 2005). "Cyclic diguanylate is a ubiquitous signaling molecule in bacteria: insights into biochemistry of the GGDEF protein domain". J. Bacteriol. 187 (5): 1792–8. doi:10.1128/JB.187.5.1792-1798.2005. PMC   1064016 . PMID   15716451.
  3. Chan C, Paul R, Samoray D, Amiot NC, Giese B, Jenal U, Schirmer T (December 2004). "Structural basis of activity and allosteric control of diguanylate cyclase". Proc. Natl. Acad. Sci. U.S.A. 101 (49): 17084–9. Bibcode:2004PNAS..10117084C. doi: 10.1073/pnas.0406134101 . PMC   535365 . PMID   15569936.
This article incorporates text from the public domain Pfam and InterPro: IPR000160