Thiazole synthase

Thiazole synthase
Thiazole synthase oktamer, Saccharomyces cerevisiae
Identifiers
EC no.	2.8.1.10
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Thiazole synthase (EC 2.8.1.10, thiG (gene)) is an enzyme with systematic name 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase. ^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] ${\displaystyle \rightleftharpoons }$ 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H₂O

H₂S can provide the sulfur in vitro.

References

1. Park JH, Dorrestein PC, Zhai H, Kinsland C, McLafferty FW, Begley TP (October 2003). "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)". Biochemistry. 42 (42): 12430–8. doi:10.1021/bi034902z. PMID   14567704.
2. Dorrestein PC, Zhai H, McLafferty FW, Begley TP (October 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS". Chemistry & Biology. 11 (10): 1373–81. doi: 10.1016/j.chembiol.2004.08.009 . PMID   15489164.
3. Dorrestein PC, Zhai H, Taylor SV, McLafferty FW, Begley TP (March 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase". Journal of the American Chemical Society. 126 (10): 3091–6. Bibcode:2004JAChS.126.3091D. doi:10.1021/ja039616p. PMID   15012138.
4. Settembre EC, Dorrestein PC, Zhai H, Chatterjee A, McLafferty FW, Begley TP, Ealick SE (September 2004). "Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex". Biochemistry. 43 (37): 11647–57. doi:10.1021/bi0488911. PMID   15362849.
5. Hazra A, Chatterjee A, Begley TP (March 2009). "Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction". Journal of the American Chemical Society. 131 (9): 3225–9. Bibcode:2009JAChS.131.3225H. doi:10.1021/ja806752h. PMC   2765510 . PMID   19216519.
6. Hazra AB, Han Y, Chatterjee A, Zhang Y, Lai RY, Ealick SE, Begley TP (June 2011). "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase". Journal of the American Chemical Society. 133 (24): 9311–9. Bibcode:2011JAChS.133.9311H. doi:10.1021/ja1110514. PMC   3116082 . PMID   21534620.

External links

• Thiazole+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)