MYLK

MYLK
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2CQV, 2K0F, 2YR3
Identifiers
Aliases	MYLK , AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK, myosin light chain kinase, MMIHS
External IDs	OMIM: 600922 MGI: 894806 HomoloGene: 14202 GeneCards: MYLK
Gene location (Human) Chr. Chromosome 3 (human) [1] Band 3q21.1 Start 123,610,049 bp [1] End 123,884,331 bp [1]
Gene location (Mouse) Chr. Chromosome 16 (mouse) [2] Band 16|16 B3 Start 34,745,210 bp [2] End 35,002,420 bp [2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • nucleotide binding • protein kinase activity • metal ion binding • calmodulin binding • myosin light chain kinase activity • kinase activity • protein serine/threonine kinase activity • GO:0001948 protein binding • actin binding • ATP binding Cellular component • cytoplasm • cytosol • cell projection • stress fiber • cleavage furrow • cytoskeleton • lamellipodium • cell membrane • actin cytoskeleton Biological process • smooth muscle contraction • muscle contraction • phosphorylation • tonic smooth muscle contraction • positive regulation of cell migration • positive regulation of wound healing • bleb assembly • cellular hypotonic response • protein phosphorylation • aorta smooth muscle tissue morphogenesis • positive regulation of calcium ion transport Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4638	107589
Ensembl	ENSG00000065534	ENSMUSG00000022836
UniProt	Q15746	Q6PDN3
RefSeq (mRNA)	NM_001321309 NM_005965 NM_053025 NM_053026 NM_053027 NM_053028 NM_053029 NM_053030 NM_053031 NM_053032	NM_139300
RefSeq (protein)	NP_001308238 NP_444253 NP_444254 NP_444255 NP_444256 NP_444259 NP_444260	NP_647461
Location (UCSC)	Chr 3: 123.61 – 123.88 Mb	Chr 16: 34.75 – 35 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene. ^[5]

Function

This gene, a muscle member of the immunoglobulin superfamily, encodes a myosin light-chain kinase, which is a calcium-/calmodulin-dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. ^[6]

See also

• Myosin light-chain kinase

References

1. GRCh38: Ensembl release 89: ENSG00000065534 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000022836 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Potier MC, Chelot E, Pekarsky Y, Gardiner K, Rossier J, Turnell WG (Mar 1996). "The human myosin light chain kinase (MLCK) from hippocampus: cloning, sequencing, expression, and localization to 3qcen-q21". Genomics. 29 (3): 562–70. doi:10.1006/geno.1995.9965. PMID   8575746.
6. "Entrez Gene: MYLK myosin, light chain kinase".

Further reading

• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC   139241 . PMID   12477932.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Clayburgh DR, Rosen S, Witkowski ED, et al. (2005). "A Differentiation-dependent Splice Variant of Myosin Light Chain Kinase, MLCK1, Regulates Epithelial Tight Junction Permeability". J. Biol. Chem. 279 (53): 55506–13. doi:10.1074/jbc.M408822200. PMC   1237105 . PMID   15507455.
• Wang F, Graham WV, Wang Y, et al. (2005). "Interferon-γ and Tumor Necrosis Factor-α Synergize to Induce Intestinal Epithelial Barrier Dysfunction by Up-Regulating Myosin Light Chain Kinase Expression". Am. J. Pathol. 166 (2): 409–19. doi:10.1016/S0002-9440(10)62264-X. PMC   1237049 . PMID   15681825.
• Russo JM, Florian P, Shen L, et al. (2005). "Distinct Temporal-Spatial Roles for Rho Kinase and Myosin Light Chain Kinase in Epithelial Purse-String Wound Closure". Gastroenterology. 128 (4): 987–1001. doi:10.1053/j.gastro.2005.01.004. PMC   1237051 . PMID   15825080.
• Shimizu S, Yoshida T, Wakamori M, et al. (2006). "Ca2+–calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells". J. Physiol. 570 (Pt 2): 219–35. doi:10.1113/jphysiol.2005.097998. PMC   1464317 . PMID   16284075.
• Kim MT, Kim BJ, Lee JH, et al. (2006). "Involvement of calmodulin and myosin light chain kinase in activation of mTRPC5 expressed in HEK cells". Am. J. Physiol., Cell Physiol. 290 (4): C1031–40. doi:10.1152/ajpcell.00602.2004. PMID   16306123.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC   1356129 . PMID   16344560.
• Connell LE, Helfman DM (2007). "Myosin light chain kinase plays a role in the regulation of epithelial cell survival". J. Cell Sci. 119 (Pt 11): 2269–81. doi: 10.1242/jcs.02926 . PMID   16723733.
• Seguchi O, Takashima S, Yamazaki S, et al. (2007). "A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart". J. Clin. Invest. 117 (10): 2812–24. doi:10.1172/JCI30804. PMC   1978424 . PMID   17885681.

External links

• MYLK+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.