Cyanothece

Last updated

Cyanothece
SAG 87.79 Cyanothece aeruginosa TD 160415 001 ovl.jpg
Cyanothece aeruginosa
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Bacteria
Phylum: Cyanobacteria
Class: Cyanophyceae
Order: Oscillatoriales
Family: Cyanothecaceae
Komárek et al. 2014 [1]
Genus: Cyanothece
Komárek 1976

Cyanothece is a genus of unicellular, diazotrophic, oxygenic photosynthesizing cyanobacteria.

Contents

Modern organisms and cellular organization

In 1976, Jiří Komárek defined the prokaryotic cyanobacteria genus Cyanothece as distinct from Synechococcus NAG 1949. [2] Organisms in both genera share characteristics in addition to being oxygenic phototrophs. They are both unicellular, forming aggregates, but not found in mucilaginous colonies. [2] [3] They may have a thin mucilage layer around each cell. [2] Both genera also divide by binary fission along an axis perpendicular to the cell's longitudinal axis. [2] [3] [4]

A handful of characteristics distinguish the two genera. While Synechococcus species are usually cylindrical, Cyanothece species are normally oval and longer than 3 μm., [2] [5] [6] [7] Cyanothece’s outer cell wall layer is relatively thick and contains spherical, glassy vesicles whose function has yet to be defined. [2] Cyanothece’s nucleoids are spread loosely throughout the cell, with a net-like appearance. [2] [3] Instead of concentric thylakoid membranes that share a center or axis, Cyanothece’s exhibit short, wavy and radially arranged., [3] [7] All Cyanothece had nitrogenase activity at one time; although some strains have lost the necessary genes. [5] During nitrogen-fixing conditions, Cyanothece creates inclusion storage bodies under the control of a circadian rhythm. [7]

Evolutionary history

Between 2.5 and 3.0 billion years ago, cyanobacteria started using the energy from light to split water, releasing oxygen into the anaerobic, reducing environment. [5] [8] Parts of this ancient cyanobacterial metabolism are still maintained today. [8] Bandyopadhyay et al. 2011 created a phylogenic tree for cyanobacteria using 226 homolog protein groups. They grouped five of the six major Cyanothece strains (PCC 7424, PCC 7822, ATCC 51142, PCC 8801, PCC 8802) as belonging to a single clade, but had Cyanothece sp PCC 7425 branched off earlier. PCC 7425's nitrogenase cluster is arranged differently from the other five strains and can only fix nitrogen anaerobically. [5] Most other cyanobacteria may have lost their ability to fix nitrogen. As Earth's climate became more oxidated, the process of fixing nitrogen became unfavorable, and natural selection eliminated some of the necessary genes for the nitrogenase protein complex to increase evolutionary fitness. [5] [6]

Photosynthesis/pigments

Cyanobacteria turn energy from the sun into chemical energy through oxygenic photosynthesis. Their light-harvesting complex that captures the photons usually includes the pigments chlorophyll a and phycocyanin. A cyanobacterium's typical blue-green color is a result of the combination of these two pigments. Three Cyanothece strains, sp. PCC 7424, 7822 and 8801, have the additional pigment phycoerythrin, which expands the wavelengths of light these species use for energy. Phycoerythrin also gives these three species a brownish-green color. [5] [9]

The rate of oxygen created by photosystem II is much higher when Cyanothece does not fix nitrogen (when the medium is nitrogen-replete). [10] The genera's circadian rhythm controls photosynthetic oxygen generation by regulating when the proteins for their photosynthetic machinery are produced. [11] [12] This diurnal oscillation occurs even when the organisms are kept in the light continuously [13] [14] or in the dark continuously. [14] Photosynthesis is downregulated when the nitrogen-fixing enzyme, nitrogenase, is upregulated. Decreasing the oxygen in the cell allows the oxygen-sensitive nitrogenase to fix nitrogen from the air for the organism's needs. [5] [14]

Metabolism, biosynthesis, symbiosis

Cyanothece balances the production of oxygen through photosynthesis and oxygen-sensitive nitrogen fixation and fermentation all in one cell. They accomplish this by separating the two processes in time under the control of their circadian rhythm. [5] [13] During the day, they use the energy harnessed from photosynthesis to produce the carbohydrate glycogen, which is stored in granules. [5] [13] At night, the organisms break down the glycogen, providing the energy for nitrogen fixation. [13] In a very energy-intensive process, nitrogenase is first synthesized [13] [14] and then takes N2 from the air, combining it with protons and electrons to produce ammonia and hydrogen gas. The organisms also store cyanophycin, a nitrogen-reserve molecule which is a polymer of arginine and asparagine, for use by the organism during the day. [5] Different Cyanothece species metabolize nitrogen-containing compounds through a variety of pathways; all have an arginine decarboxylase, but vary after that point. [5]

To provide the anoxic environment needed by nitrogenase, Cyanothece boosts its respiration as night begins by using its glycogen stores [12] while turning off photosynthesis. [8] [13] In addition, the organisms produce peroxidases and catalases which help scavenge any oxygen left in the cell. [5] The circadian rhythm ensures that this occurs even when the organism is growing in continuous light [7] [13] [14] or continuous darkness. [9] [14] In the dark, the cyanobacteria act as heterotrophs, getting their energy and carbon from the medium. Cyanothece has the genes for the use of a variety of sugar molecules; [5] although glycerol is the only one that has been used successfully to grow Cyanothece in the dark. [7] [9] [10] [14] Many of the genes that are unique to the genera have homologs in anaerobic bacteria, including those responsible for formate production through mixed-acid fermentation and also fermentative lactate production. [5] Some Cyanothece species also are capable of tryptophan degradation, methionine salvage, conversion of stored lipids into carbohydrates, alkane and higher alcohol synthesis, and phosphonate metabolism. [5] They can switch between a photoautotrophic and photoheterotrophic metabolism depending on the environmental conditions that maximize their growth, employing the pathways that use the least amount of energy. [10]

Genome size, organization, and ploidy options

The genomes of many of Cyanothece species have been sequenced, ranging from 4.79 to 7.84 Mbp. Between 4367 and 6642 coding sequences are an amalgamation of genes encoding capabilities for fermentation and aerobic nitrogen fixation (like filamentous cyanobacteria). [5] Unusually, the genes for nitrogen fixation are in a large, contiguous cluster (under the control of multiple promoters), [15] including genes for the uptake hydrogenase, regulators, and transporters. [5] The organism's robust circadian rhythm is apparent in the co-ordination of transcription of correlated processes. [5] Using microarrays, about 30% of 5000 genes tested exhibited diurnal oscillations in 12-hour light/dark conditions, while 10% continued the behavior in continuous light. [8] About 1,705 of the gene groups are >99.5% homologous with other cyanobacteria genera, largely Microcystis and filamentous, nitrogen-fixing strains. Typical GC content is about 40%. [5] [7] Cyanothece species also have three to six plasmids ranging between 10 and 330 kb. [5] Unique to some species of this genus is one to three linear pieces of DNA. [5] [15] The linear DNA encodes enzymes for glucose and pyruvate metabolism [15] (recall that glycerol is the only organic carbon source on which Cyanothece has grown successfully [10] ), lactate fermentation, [8] transposons, and CRISPR proteins. [5] Cyanothece species do not typically use homologous recombination, which greatly hinders genetic manipulation; an exception is Cyanothece sp. PCC 7822 in which gene knock-outs can be generated. [9]

Cell size, growth patterns, sex

Cyanothece species are normally oval and longer than 3 μm. [2] [5] [6] [7] They double in 10 to 14 hours in the presence of nitrate, when they do not need to use energy to fix nitrogen, and 16 to 20 hours when fixing nitrogen. [7] They divide by binary fission in one plane that is perpendicular to their longitudinal axis. [2] [6] Daughter cells remain joined for just a short time after division. [2] Cell division does not proceed until the daughter cells reach their mature size and original shape. [2] [3]

Habitat range

Cyanothece has been found in a variety of environments all over the world. One point in common is that the pH is usually lower than 7. [2] Typically they are associated with water in benthic marine environments, [5] rice fields, [5] acidic marshes, [4] peaty bogs, [2] intertidal zones, [4] [7] moors [3] and clear lakes, [3] but sometimes are found in mountain soils. [3]

Walls and resting cysts

Cyanothece species have a thin mucilaginous layer around a thick outer wall that contains spherical, glassy vesicles of unknown function. [2] [3] They have been shown to secrete abundant Extracellular polymeric substances (EPS). [16] The EPS has been used to sequester metals from industrial waste, with more than 90% of Ni2+, Cu2+, and Co2+ removed. [16]

Storage products

Cyanothece stores the products of carbon fixation as glycogen granules which they use as an energy source during the "night". [5] [8] [9] [12] These granules form between the thylakoid membranes. [7] The granules are rapidly consumed to boost respiration, so remove the oxygen from the cell at the onset of nitrogen fixation. [8] The carboxysomes contain the carbon-fixing enzyme rubisco and a carbon-concentrating system to boost the enzyme's efficiency. The nitrogen product, cyanophycin, is stored as a granule during nitrogen fixation and is metabolized during the "day". [5] [8] [14]

Motility

Cyanothece species are not flagellated. A twitching motility protein for sp. PCC 8802 is annotated on the protein database UniProtKB. [17]

Single-celled vs. multicelled

Cyanothece species are unicellular. [2] [5] [6] They can be found as free aggregates, [2] but have never been found as a chain. [6]

Hydrogen production

Biohydrogen is being investigated as a clean and renewable energy source. Two main enzymes produce hydrogen in microbes, hydrogenase and nitrogenase; Cyanothece has both enzymes. [18] The nitrogenase fixes nitrogen, releasing hydrogen as a byproduct. The two different hydrogenase enzymes are an uptake hydrogenase associated with the nitrogenase and a bidirectional hydrogenase. When cultures are entrained in light-dark cycles, the nitrogenase and uptake hydrogenase are both active during the "night", with many copies per cell. [8] Western blots show only a few copies of the bidirectional hydrogenase occur at any time. [8] About 300 μmol H2/(mg Chl h) was produced by sp. ATCC 51142 from cultures that were grown in continuous light at 30 μmol photons/(m2s), anaerobic conditions, 50 mM glycerol, and without any nitrate (so the nitrogenase was active). [18] Glycerol in the growth medium reduces the need for carbon fixation, leaving more energy for nitrogen fixation and hydrogen production. [10] It was shown by acetylene reduction that hydrogen generation and nitrogen fixation were directly proportional. [18] A parallel study has demonstrated concomitant and uninterrupted production of both H2 and O2 in continuously illuminated photobioreactor cultures, upon nitrogen-deprivation of ammonium-limited chemostat growth. [19] Further work on improving the supply of protons and electrons to nitrogenase, as well as protecting it from oxygen could stimulate even better rates.

See also

Related Research Articles

Nitrogen fixation is a chemical process by which molecular nitrogen (N
2), which has a strong triple covalent bond, is converted into ammonia (NH
3) or related nitrogenous compounds, typically in soil or aquatic systems but also in industry. The nitrogen in air is molecular dinitrogen, a relatively nonreactive molecule that is metabolically useless to all but a few microorganisms. Biological nitrogen fixation or diazotrophy is an important microbe-mediated process that converts dinitrogen (N2) gas to ammonia (NH3) using the nitrogenase protein complex (Nif).

<span class="mw-page-title-main">Cyanobacteria</span> Phylum of photosynthesising prokaryotes

Cyanobacteria, also called Cyanobacteriota or Cyanophyta, are a phylum of gram-negative bacteria that obtain energy via photosynthesis. The name cyanobacteria refers to their color, which similarly forms the basis of cyanobacteria's common name, blue-green algae, although they are not usually scientifically classified as algae. They appear to have originated in a freshwater or terrestrial environment. Sericytochromatia, the proposed name of the paraphyletic and most basal group, is the ancestor of both the non-photosynthetic group Melainabacteria and the photosynthetic cyanobacteria, also called Oxyphotobacteria.

<i>Trichodesmium</i> Genus of bacteria

Trichodesmium, also called sea sawdust, is a genus of filamentous cyanobacteria. They are found in nutrient poor tropical and subtropical ocean waters. Trichodesmium is a diazotroph; that is, it fixes atmospheric nitrogen into ammonium, a nutrient used by other organisms. Trichodesmium is thought to fix nitrogen on such a scale that it accounts for almost half of the nitrogen fixation in marine systems globally. Trichodesmium is the only known diazotroph able to fix nitrogen in daylight under aerobic conditions without the use of heterocysts.

<span class="mw-page-title-main">Heterocyst</span>

Heterocysts or heterocytes are specialized nitrogen-fixing cells formed during nitrogen starvation by some filamentous cyanobacteria, such as Nostoc punctiforme, Cylindrospermum stagnale, and Anabaena sphaerica. They fix nitrogen from dinitrogen (N2) in the air using the enzyme nitrogenase, in order to provide the cells in the filament with nitrogen for biosynthesis.

Diazotrophs are bacteria and archaea that fix gaseous nitrogen in the atmosphere into a more usable form such as ammonia.

<i>Azotobacter</i> Genus of bacteria

Azotobacter is a genus of usually motile, oval or spherical bacteria that form thick-walled cysts and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil. In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, Azotobacter chroococcum, was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. Azotobacter species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants.

<i>Anabaena</i> Genus of bacteria

Anabaena is a genus of filamentous cyanobacteria that exist as plankton. They are known for nitrogen-fixing abilities, and they form symbiotic relationships with certain plants, such as the mosquito fern. They are one of four genera of cyanobacteria that produce neurotoxins, which are harmful to local wildlife, as well as farm animals and pets. Production of these neurotoxins is assumed to be an input into its symbiotic relationships, protecting the plant from grazing pressure.

<span class="mw-page-title-main">Biohydrogen</span>

Biohydrogen is H2 that is produced biologically. Interest is high in this technology because H2 is a clean fuel and can be readily produced from certain kinds of biomass, including biological waste. Furthermore some photosynthetic microorganisms are capable to produce H2 directly from water splitting using light as energy source.

<i>Synechococcus</i> Genus of bacteria

Synechococcus is a unicellular cyanobacterium that is very widespread in the marine environment. Its size varies from 0.8 to 1.5 µm. The photosynthetic coccoid cells are preferentially found in well–lit surface waters where it can be very abundant. Many freshwater species of Synechococcus have also been described.

Hydrogen-oxidizing bacteria are a group of facultative autotrophs that can use hydrogen as an electron donor. They can be divided into aerobes and anaerobes. The former use hydrogen as an electron donor and oxygen as an acceptor while the latter use sulphate or nitrogen dioxide as electron acceptors. Species of both types have been isolated from a variety of environments, including fresh waters, sediments, soils, activated sludge, hot springs, hydrothermal vents and percolating water.

Synechocystis sp. PCC6803 is a strain of unicellular, freshwater cyanobacteria. Synechocystis sp. PCC6803 is capable of both phototrophic growth by oxygenic photosynthesis during light periods and heterotrophic growth by glycolysis and oxidative phosphorylation during dark periods. Gene expression is regulated by a circadian clock and the organism can effectively anticipate transitions between the light and dark phases.

Cyanobionts are cyanobacteria that live in symbiosis with a wide range of organisms such as terrestrial or aquatic plants; as well as, algal and fungal species. They can reside within extracellular or intracellular structures of the host. In order for a cyanobacterium to successfully form a symbiotic relationship, it must be able to exchange signals with the host, overcome defense mounted by the host, be capable of hormogonia formation, chemotaxis, heterocyst formation, as well as possess adequate resilience to reside in host tissue which may present extreme conditions, such as low oxygen levels, and/or acidic mucilage. The most well-known plant-associated cyanobionts belong to the genus Nostoc. With the ability to differentiate into several cell types that have various functions, members of the genus Nostoc have the morphological plasticity, flexibility and adaptability to adjust to a wide range of environmental conditions, contributing to its high capacity to form symbiotic relationships with other organisms. Several cyanobionts involved with fungi and marine organisms also belong to the genera Richelia, Calothrix, Synechocystis, Aphanocapsa and Anabaena, as well as the species Oscillatoria spongeliae. Although there are many documented symbioses between cyanobacteria and marine organisms, little is known about the nature of many of these symbioses. The possibility of discovering more novel symbiotic relationships is apparent from preliminary microscopic observations.

CandidatusAtelocyanobacterium thalassa, also referred to as UCYN-A, is a diazotrophic species of cyanobacteria commonly found in measurable quantities throughout the world's oceans and some seas. Members of A. thalassa are spheroid in shape and are 1-2µm in diameter, and provide nitrogen to ocean regions by fixing non biologically available atmospheric nitrogen into biologically available ammonium that other marine microorganisms can use. Unlike many other cyanobacteria, the genome of A. thalassa does not contain genes for RuBisCO, photosystem II, or the TCA cycle. Consequently, A. thalassa lacks the ability to fix carbon via photosynthesis. Some genes specific to the cyanobacteria group are also absent from the A. thalassa genome despite being an evolutionary descendant of this group. With the inability to fix their own carbon, A. thalassa are obligate symbionts that have been found within photosynthetic picoeukaryote algae. Most notably, the UCYN-A2 sublineage has been observed as an endosymbiont in the alga Braarudosphaera bigelowii with a minimum of 1-2 endosymbionts per host. A. thalassa fixes nitrogen for the algae, while the algae provide carbon for A. thalassa through photosynthesis. There are many sublineages of A. thalassa that are distributed across a wide range of marine environments and host organisms. It appears that some sublineages of A. thalassa have a preference for oligotrophic ocean waters while other sublineages prefer coastal waters. Much is still unknown about all of A. thalassa's hosts and host preferences.

Raphidiopsis raciborskii is a freshwater cyanobacterium.)

<span class="mw-page-title-main">Cyanophycinase</span> Class of enzymes

Cyanophycinase (EC 3.4.15.6, cyanophycin degrading enzyme, beta-Asp-Arg hydrolysing enzyme, CGPase, CphB, CphE, cyanophycin granule polypeptidase, extracellular CGPase) is an enzyme. It catalyses the following chemical reaction

<i>Crocosphaera watsonii</i> Species of bacterium

Crocosphaera watsonii is an isolate of a species of unicellular diazotrophic marine cyanobacteria which represent less than 0.1% of the marine microbial population. They thrive in offshore, open-ocean oligotrophic regions where the waters are warmer than 24 degrees Celsius. Crocosphaera watsonii cell density can exceed 1,000 cells per milliliter within the euphotic zone; however, their growth may be limited by the concentration of phosphorus. Crocosphaera watsonii are able to contribute to the oceanic carbon and nitrogen budgets in tropical oceans due to their size, abundance, and rapid growth rate. Crocosphaera watsonii are unicellular nitrogen fixers that fix atmospheric nitrogen to ammonia during the night and contribute to new nitrogen in the oceans. They are a major source of nitrogen to open-ocean systems. Nitrogen fixation is important in the oceans as it not only allows phytoplankton to continue growing when nitrogen and ammonium are in very low supply but it also replenishes other forms of nitrogen, thus fertilizing the ocean and allowing more phytoplankton growth.

Trichodesmium thiebautii is a cyanobacteria that is often found in open oceans of tropical and subtropical regions and is known to be a contributor to large oceanic surface blooms. This microbial species is a diazotroph, meaning it fixes nitrogen gas (N2), but it does so without the use of heterocysts. T. thiebautii is able to simultaneously perform oxygenic photosynthesis. T. thiebautii was discovered in 1892 by M.A. Gomont. T. thiebautii are important for nutrient cycling in marine habitats because of their ability to fix N2, a limiting nutrient in ocean ecosystems.

<i>Synechocystis</i> Genus of bacteria

Synechocystis is a genus of unicellular, freshwater cyanobacteria in the family Merismopediaceae. It includes a strain, Synechocystis sp. PCC 6803, which is a well studied model organism.

<i>Synechococcus elongatus</i> Species of bacterium

Synechococcus elongatus is a unicellular cyanobacterium that has a rapid autotrophic growth comparable to yeast. Its ability to grow rapidly using sunlight has implications for biotechnological applications, especially when incorporating genetic modification.

Alexander Glazer was a professor of the Graduate School in the Department of Molecular and Cell Biology at the University of California, Berkeley. He had a passion for protein chemistry and structure function relationships. He also had a longstanding interest in light-harvesting complexes in cyanobacteria and red algae called phycobilisomes. He had also spent more than 10 years working on the human genome project where he has investigated methods for DNA detection and sequencing which most notably includes the development of fluorescent reagents involved in cell labeling. Most recently, he had focused his studies on issues in environmental sciences. He died on July 18, 2021, in Orinda, California

References

  1. ↑ Komárek J, Kaštovský J, Mareš J, Johansen JR (2014). "Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach" (PDF). Preslia. 86: 295–335.
  2. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Komarek, J.; Cepak, V. (1998). "Cytomorphological characters supporting the taxonomic validity ofCyanothece (Cyanoprokaryota)". Plant Systematics and Evolution. 210 (1–2): 25–39. doi:10.1007/BF00984725. S2CID   6349520.
  3. 1 2 3 4 5 6 7 8 9 Porta, D.; Rippka, R.; Hernandez-Marine, M. (2000). "Unusual ultrastructural features in three strains of Cyanothece (cyanobacteria)". Archives of Microbiology. 173 (2): 154–163. doi:10.1007/s002039900126. PMID   10795687. S2CID   19444708.
  4. 1 2 3 Komárek, Jiří; Cepák, Vladislav; Kaštovský, Jan; Sulek, Josef (1 August 2004). "What are the cyanobacterial genera Cyanothece and Cyanobacterium? Contribution to the combined molecular and phenotype taxonomic evaluation of cyanobacterial diversity". Algological Studies. 113 (1): 1–36. doi:10.1127/1864-1318/2004/0113-0001.
  5. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Bandyopadhyay, A.; Elvitigala, T.; Welsh, E.; Stockel, J.; Liberton, M.; Min, H.; Sherman, L. A.; Pakrasi, H. B. (4 October 2011). "Novel Metabolic Attributes of the Genus Cyanothece, Comprising a Group of Unicellular Nitrogen-Fixing Cyanobacteria". mBio. 2 (5): e00214-11–e00214-11. doi:10.1128/mBio.00214-11. PMC   3187577 . PMID   21972240.
  6. 1 2 3 4 5 6 Turner, S.; Huang, T.-C.; Chaw, S.-M. (2001). "Molecular phylogeny of nitrogen-fixing unicellular cyanobacteria". Botanical Bulletin of Academia Sinica. 42.
  7. 1 2 3 4 5 6 7 8 9 10 Reddy, K.J.; Haskell, J.B.; Sherman, D.M.; Sherman, L.A. (1993). "Unicellular, aerobic nitrogen-fixing cyanobacteria of the genus Cyanothece". Journal of Bacteriology. 175 (5): 1284–1292. doi:10.1128/JB.175.5.1284-1292.1993. PMC   193213 . PMID   8444791.
  8. 1 2 3 4 5 6 7 8 9 10 Sherman, L.A.; Min, H.; Toepel, J.; Pakrasi, H.B. (2010). "Better Living Through Cyanothece – Unicellular Diazotrophic Cyanobacteria with Highly Versatile Metabolic Systems". Recent Advances in Phototrophic Prokaryotes. Advances in Experimental Medicine and Biology. Vol. 675. pp. 275–290. doi:10.1007/978-1-4419-1528-3_16. ISBN   978-1-4419-1527-6. PMID   20532747.
  9. 1 2 3 4 5 Aryal, U. K.; Callister, S. J.; Mishra, S.; Zhang, X.; Shutthanandan, J. I.; Angel, T. E.; Shukla, A. K.; Monroe, M. E.; Moore, R. J.; Koppenaal, D. W.; Smith, R. D.; Sherman, L. (30 November 2012). "Proteome Analyses of Strains ATCC 51142 and PCC 7822 of the Diazotrophic Cyanobacterium Cyanothece sp. under Culture Conditions Resulting in Enhanced H2 Production". Applied and Environmental Microbiology. 79 (4): 1070–1077. doi:10.1128/AEM.02864-12. PMC   3568600 . PMID   23204418.
  10. 1 2 3 4 5 Feng, X.; Bandyopadhyay, A.; Berla, B.; Page, L.; Wu, B.; Pakrasi, H. B.; Tang, Y. J. (29 April 2010). "Mixotrophic and photoheterotrophic metabolism in Cyanothece sp. ATCC 51142 under continuous light". Microbiology. 156 (8): 2566–2574. doi: 10.1099/mic.0.038232-0 . PMID   20430816.
  11. ↑ Basu, Subhayu; Gerchman, Yoram; Collins, Cynthia H.; Arnold, Frances H.; Weiss, Ron (28 April 2005). "A synthetic multicellular system for programmed pattern formation" (PDF). Nature. 434 (7037): 1130–1134. doi:10.1038/nature03461. PMID   15858574. S2CID   4370309.
  12. 1 2 3 Schneegurt, M.A.; Sherman, D.M.; Nayar, S.; Sherman, L.A. (1994). "Oscillating behavior of carbohydrate granule formation and dinitrogen fixation in the cyanobacterium Cyanothece sp. strain ATCC 51142". Journal of Bacteriology. 176 (6): 1586–1597. doi:10.1128/JB.176.6.1586-1597.1994. PMC   205243 . PMID   8132452.
  13. 1 2 3 4 5 6 7 Colon-Lopez, M.S.; Sherman, D.M.; Sherman, L.A. (1997). "Transcriptional and translational regulation of nitrogenase in light-dark- and continuous-light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142". Journal of Bacteriology. 179 (13): 4319–4327. doi:10.1128/JB.179.13.4319-4327.1997. PMC   179256 . PMID   9209050.
  14. 1 2 3 4 5 6 7 8 Schneegurt, Mark A.; Tucker, Don L.; Ondr, Jennifer K.; Sherman, Debra M.; Sherman, Louis A. (9 February 2000). "Metabolic rhythms of a diazotrophic cyanobacterium, cyanothece sp. strain atcc 51142, heterotrophically grown in continuous dark". Journal of Phycology. 36 (1): 107–117. doi:10.1046/j.1529-8817.2000.99152.x. S2CID   84207562.
  15. 1 2 3 Welsh, E. A.; Liberton, M.; Stockel, J.; Loh, T.; Elvitigala, T.; Wang, C.; Wollam, A.; Fulton, R. S.; Clifton, S. W.; Jacobs, J. M.; Aurora, R.; Ghosh, B. K.; Sherman, L. A.; Smith, R. D.; Wilson, R. K.; Pakrasi, H. B. (23 September 2008). "The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle". Proceedings of the National Academy of Sciences. 105 (39): 15094–15099. doi: 10.1073/pnas.0805418105 . PMC   2567498 . PMID   18812508.
  16. 1 2 Shah, V.; Ray, A.; Garg, N.; Madamwar, D. (2000). "Characterization of the extracellular polysaccharide produced by a marine cyanobacterium, Cyanothece sp. ATCC 51142, and its exploitation toward metal removal from solutions". Current Microbiology. 40 (4): 274–278. doi:10.1007/s002849910054. PMID   10688698. S2CID   22352524.
  17. ↑ "UniProt Knowledgebase". Uniprot. Retrieved August 26, 2020.
  18. 1 2 3 Min, H.; Sherman, L. A. (7 May 2010). "Hydrogen Production by the Unicellular, Diazotrophic Cyanobacterium Cyanothece sp. Strain ATCC 51142 under Conditions of Continuous Light". Applied and Environmental Microbiology. 76 (13): 4293–4301. doi:10.1128/AEM.00146-10. PMC   2897434 . PMID   20453150.
  19. ↑ Melnicki, M. R.; Pinchuk, G. E.; Hill, E. A.; Kucek, L. A.; Fredrickson, J. K.; Konopka, A.; Beliaev, A. S. (2012). "Sustained H2 Production Driven by Photosynthetic Water Splitting in a Unicellular Cyanobacterium". mBio. 3 (4): e00197-12–e00197-12. doi:10.1128/mBio.00197-12. ISSN   2150-7511. PMC   3419522 . PMID   22872781.
This page is based on this Wikipedia article
Text is available under the CC BY-SA 4.0 license; additional terms may apply.
Images, videos and audio are available under their respective licenses.