Mycobacterium pseudoshottsii

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Mycobacterium pseudoshottsii
Scientific classification
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pseudoshottsii
Binomial name
Mycobacterium pseudoshottsii

Mycobacterium pseudoshottsii, a slowly growing chromogenic species was isolated from Chesapeake Bay striped bass ( Morone saxatilis ) during an epizootic of mycobacteriosis. [1]

Taxonomic name not approved yet.

Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those of the genus Mycobacterium . Biochemical reactions, growth characteristics and mycolic acid profiles (HPLC) resembled those of Mycobacterium shottsii , a non-pigmented mycobacterium also isolated during the same epizootic. Sequencing of the 16S rRNA genes, the gene encoding the exported repeated protein (erp) and the gene encoding the 65 kDa heat-shock protein (hsp65) and restriction enzyme analysis of the hsp65 gene demonstrated that this group of isolates is unique.

Insertion sequences associated with Mycobacterium ulcerans , IS2404 and IS2606, were detected by PCR. These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 °C, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid (1 μg ml–1), p-nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide. On the basis of this polyphasic study, it is proposed that these isolates represent a novel species, Mycobacterium pseudoshottsii sp. nov. The type strain, L15T, has been deposited in the American Type Culture Collection as ATCC BAA-883T and the National Collection of Type Cultures (UK) as NCTC 13318T.

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References

  1. Rhodes MW, Kator H, McNabb A, et al. (May 2005). "Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)". Int. J. Syst. Evol. Microbiol. 55 (Pt 3): 1139–47. doi: 10.1099/ijs.0.63343-0 . PMID   15879246.