A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology.
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss Physcomitrella patens. Different types of media are used for growing different types of cells.
Mycolicibacterium alvei is a species of the phylum Actinomycetota, belonging to the genus Mycolicibacterium.
Mycobacterium botniense is a slowly growing Mycobacterium, which produces a yellow pigment. It was first isolated from a stream of water. M. botniense is most closely related to Mycobacterium xenopi. Etymology: botniense; of Botnia, referring to the Latin name of the province of Finland from which the isolation was made.
Mycobacterium branderi is a slowly growing, nonchromogenic Mycobacterium first isolated from patients in Finland. Etymology: of Brander, referring to Eljas Brander, the former head of the Tuberculosis Laboratory of the National Public Health Institute, Finland, who collected the strains.
Mycobacterium cookii is a species of the phylum Actinomycetota, belonging to the genus Mycobacterium.
Mycobacterium elephantis, a bacterium of the family Mycobacteriaceae, was discovered and isolated from a deceased elephant near India and may be linked to respiratory dysfunction. Organisms in the genus Mycobacterium are known to be aerobic and non-motile. Organisms within Mycobacterium belong to either the rapid growing group or the slow growing group. M. elephantis is classified as a rapid grower and relates most closely to Mycobacterium confluentis and Mycobacterium phlei.
Mycobacterium fallax is a species of the phylum Actinomycetota, belonging to the genus Mycobacterium.
Mycobacterium gordonae is a species of Mycobacterium named for Ruth E. Gordon. It is a species of the phylum Actinomycetota, belonging to the genus Mycobacterium.
Mycobacterium haemophilum is a species of the phylum Actinomycetota, belonging to the genus Mycobacterium.
Mycobacterium heidelbergense is a Gram-positive, nonmotile, acid-fast coccobacillus. It is a species of the phylum Actinomycetota, belonging to the genus Mycobacterium.
Mycolicibacter hiberniae is a species of bacteria in the phylum Actinomycetota.
Mycobacterium interjectum is a species of the phylum Actinomycetota, belonging to the genus Mycobacterium.
Mycobacterium intermedium is a species of the phylum Actinomycetota, belonging to the genus Mycobacterium.
Mycobacterium kubicae is a Gram-positive, nonmotile and acid-fast bacterial species. Cells are typically rod-shaped, with some coccoid forms. Colonies of M. kubicae on solid media are generally smooth and domed, with a yellow scotochromogenic pigment. On Löwenstein-Jensen media they appear film-like. This species is not known to be pathogenic to humans. The species is named after American mycobacteriologist George Kubica.
Mycobacterium lacus is a species of bacteria in the genus Mycobacterium known to be a causative agent in immunocompetent individuals.
Löwenstein–Jensen medium, more commonly known as LJ medium, is a growth medium specially used for culture of Mycobacterium species, notably Mycobacterium tuberculosis.
Mycobacteria Growth Indicator Tube (MGIT) is intended for the culture, detection and recovery of mycobacteria. The MGIT Mycobacteria Growth Indicator Tube contains 7 mL of modified Middlebrook 7H9 Broth base. The complete medium, with OADC enrichment and PANTA antibiotic mixture, is one of the most commonly used liquid media for the cultivation of mycobacteria.
Middlebrook 7H9 broth is a liquid growth medium specially used for culture of Mycobacterium species, notably Mycobacterium tuberculosis.
In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes, as present in the environment, for example in water or soil, or from living beings with skin flora, oral flora or gut flora, in order to identify the microbe(s) of interest. Historically, the laboratory techniques of isolation first developed in the field of bacteriology and parasitology, before those in virology during the 20th century.
