Biochemical oxygen demand

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BOD test bottles at the laboratory of a wastewater treatment plant BOD test bottles (biological oxygen demand) (3231600029).jpg
BOD test bottles at the laboratory of a wastewater treatment plant

Biochemical oxygen demand (also known as BOD or biological oxygen demand) is an analytical parameter representing the amount of dissolved oxygen (DO) consumed by aerobic bacteria growing on the organic material present in a water sample at a specific temperature over a specific time period. The BOD value is most commonly expressed in milligrams of oxygen consumed per liter of sample during 5 days of incubation at 20 °C and is often used as a surrogate of the degree of organic water pollution. [1]

Contents

Biochemical Oxygen Demand (BOD) reduction is used as a gauge of the effectiveness of wastewater treatment plants. BOD of wastewater effluents is used to indicate the short-term impact on the oxygen levels of the receiving water.

BOD analysis is similar in function to chemical oxygen demand (COD) analysis, in that both measure the amount of organic compounds in water. However, COD analysis is less specific, since it measures everything that can be chemically oxidized, rather than just levels of biologically oxidized organic matter.

Background

Most natural waters contain small quantities of organic compounds. Aquatic microorganisms have evolved to use some of these compounds as food. Microorganisms living in oxygenated waters use dissolved oxygen to oxidatively degrade the organic compounds, releasing energy which is used for growth and reproduction. Populations of these microorganisms tend to increase in proportion to the amount of food available. This microbial metabolism creates an oxygen demand proportional to the amount of organic compounds useful as food. Under some circumstances, microbial metabolism can consume dissolved oxygen faster than atmospheric oxygen can dissolve into the water or the autotrophic community (algae, cyanobacteria and macrophytes) can produce. Fish and aquatic insects may die when oxygen is depleted by microbial metabolism. [2]

Biochemical oxygen demand is the amount of oxygen required for microbial metabolism of organic compounds in water. This demand occurs over some variable period of time depending on temperature, nutrient concentrations, and the enzymes available to indigenous microbial populations. The amount of oxygen required to completely oxidize the organic compounds to carbon dioxide and water through generations of microbial growth, death, decay, and cannibalism is total biochemical oxygen demand (total BOD). Total BOD is of more significance to food webs than to water quality. Dissolved oxygen depletion is most likely to become evident during the initial aquatic microbial population explosion in response to a large amount of organic material. If the microbial population deoxygenates the water, however, that lack of oxygen imposes a limit on population growth of aerobic aquatic microbial organisms resulting in a longer term food surplus and oxygen deficit. [3]

A standard temperature at which BOD testing should be carried out was first proposed by the Royal Commission on Sewage Disposal in its eighth report in 1912:

(c) An effluent in order to comply with the general standard must not contain as discharged more than 3 parts per 100,000 of suspended matter, and with its suspended matters included must not take up at 65°F more than 2.0 parts per 100,000 of dissolved oxygen in 5 days. This general standard should be prescribed either by Statute or by order of the Central Authority, and should be subject to modifications by that Authority after an interval of not less than ten years.

This was later standardised at 68 °F and then 20 °C. This temperature may be significantly different from the temperature of the natural environment of the water being tested.

Although the Royal Commission on Sewage Disposal proposed 5 days as an adequate test period for rivers of the United Kingdom of Great Britain and Ireland, longer periods were investigated for North American rivers. Incubation periods of 1, 2, 5, 10 and 20 days were being used into the mid-20th century. [4] Keeping dissolved oxygen available at their chosen temperature, investigators found up to 99 percent of total BOD was exerted within 20 days, 90 percent within 10 days, and approximately 68 percent within 5 days. [5] Variable microbial population shifts to nitrifying bacteria limit test reproducibility for periods greater than 5 days. The 5-day test protocol with acceptably reproducible results emphasizing carbonaceous BOD has been endorsed by the United States Environmental Protection Agency (EPA). This 5-day BOD test result may be described as the amount of oxygen required for aquatic microorganisms to stabilize decomposable organic matter under aerobic conditions. [6] Stabilization, in this context, may be perceived in general terms as the conversion of food to living aquatic fauna. Although these fauna will continue to exert biochemical oxygen demand as they die, that tends to occur within a more stable evolved ecosystem including higher trophic levels. [3]

Taking samples from the influent raw wastewater stream for BOD measurements at a wastewater treatment plant in Haran-Al-Awamied near Damascus in Syria Influent raw wastewater (note grey colour in glass jar) (3232393634).jpg
Taking samples from the influent raw wastewater stream for BOD measurements at a wastewater treatment plant in Haran-Al-Awamied near Damascus in Syria

History

The Royal Commission on River Pollution, established in 1865, and the formation of the Royal Commission on Sewage Disposal in 1898 led to the selection in 1908 of BOD5 as the definitive test for organic pollution of rivers. Five days was chosen as an appropriate test period because this is supposedly the longest time that river water takes to travel from source to estuary in the U.K. In its sixth report the Royal Commission recommended that the standard set should be 15 parts by weight per million of water. [7] However, in the Ninth report the commission had revised the recommended standard:

An effluent taking up 2–0 parts dissolved oxygen per 100,000 would be found by a simple calculation to require dilution with at least 8 volumes of river water taking up 0.2 part if the resulting mixture was not to take up more than 0.4 part. Our experience indicated that in a large majority of cases the volume of river water would exceed 8 times the volume of effluent, and that the figure of 2–0 parts dissolved oxygen per 100,000, which had been shown to be practicable, would be a safe figure to adopt for the purposes of a general standard, taken in conjunction with the condition that the effluent should not contain more than 3–0 parts per 100,000 of suspended solids. [7]

This was the cornerstone 20:30 (BOD:Suspended Solids) + full nitrification standard which was used as a yardstick in the U.K. up to the 1970s for sewage works effluent quality.

The United States includes BOD effluent limitations in its secondary treatment regulations. Secondary sewage treatment is generally expected to remove 85 percent of the BOD measured in sewage and produce effluent BOD concentrations with a 30-day average of less than 30 mg/L and a 7-day average of less than 45 mg/L. The regulations also describe "treatment equivalent to secondary treatment" as removing 65 percent of the BOD and producing effluent BOD concentrations with a 30-day average less than 45 mg/L and a 7-day average less than 65 mg/L. [8]

Typical values

Most pristine rivers will have a 5-day carbonaceous BOD below 1 mg/L. Moderately polluted rivers may have a BOD value in the range of 2 to 8 mg/L. Rivers may be considered severely polluted when BOD values exceed 8 mg/L. [9] Municipal sewage that is efficiently treated by a three-stage process would have a value of about 20 mg/L or less. Untreated sewage varies, but averages around 600 mg/L in Europe and as low as 200 mg/L in the U.S., or where there is severe groundwater or surface water infiltration/inflow. The generally lower values in the U.S. derive from the much greater water use per capita than in other parts of the world. [1]

Use in sewage treatment

The BOD is used in measuring waste loadings to treatment plants and in evaluating the BOD-removal efficiency of such treatment systems.

Methods

Winkler published the methodology of a simple, accurate and direct dissolved oxygen analytical procedure in 1888. [10] Since that time, the analysis of dissolved oxygen levels for water has been key to the determination of surface water. The Winkler method is still one of only two analytical techniques used to calibrate oxygen electrode meters; the other procedure is based on oxygen solubility at saturation as per Henry's law.

There are two recognized methods for the measurement of dissolved oxygen for BOD and a number of other methods not currently internationally recognised as standard methods

Dilution method

Disposable BOD bottle Disposable BOD Bottles.jpg
Disposable BOD bottle
Glass BOD bottle BOD Bottle.jpg
Glass BOD bottle

This standard method is recognized by EPA, which is labeled Method 5210B in the Standard Methods for the Examination of Water and Wastewater. [11] In order to obtain BOD5, dissolved oxygen (DO) concentrations in a sample must be measured before and after the incubation period, and appropriately adjusted by the sample corresponding dilution factor. This analysis is performed using 300 mL incubation bottles in which buffered dilution water is dosed with seed microorganisms and stored for 5 days in the dark room at 20 °C to prevent DO production via photosynthesis. The bottles have traditionally been made of glass, which required cleaning and rinsing between samples. A SM 5210B approved, disposable, plastic BOD bottle is available which eliminates this step. In addition to the various dilutions of BOD samples, this procedure requires dilution water blanks, glucose glutamic acid (GGA) controls, and seed controls. The dilution water blank is used to confirm the quality of the dilution water that is used to dilute the other samples. This is necessary because impurities in the dilution water may cause significant alterations in the results. The GGA control is a standardized solution to determine the quality of the seed, where its recommended BOD5 concentration is 198 mg/L ± 30.5 mg/L. For measurement of carbonaceous BOD (cBOD), a nitrification inhibitor is added after the dilution water has been added to the sample. The inhibitor hinders the oxidation of ammonia nitrogen, which supplies the nitrogenous BOD (nBOD). When performing the BOD5 test, it is conventional practice to measure only cBOD because nitrogenous demand does not reflect the oxygen demand from organic matter. This is because nBOD is generated by the breakdown of proteins, whereas cBOD is produced by the breakdown of organic molecules.

BOD5 is calculated by:

where:

is the dissolved oxygen (DO) of the diluted solution after preparation (mg/L)
is the DO of the diluted solution after 5 day incubation (mg/L)
is the decimal dilution factor
is the DO of diluted seed sample after preparation (mg/L)
is the DO of diluted seed sample after 5 day incubation (mg/L)
is the ratio of seed volume in dilution solution to seed volume in BOD test on seed

Manometric method

This method is limited to the measurement of the oxygen consumption due only to carbonaceous oxidation. Ammonia oxidation is inhibited.

The sample is kept in a sealed container fitted with a pressure sensor. A substance that absorbs carbon dioxide (typically lithium hydroxide) is added in the container above the sample level. The sample is stored in conditions identical to the dilution method. Oxygen is consumed and, as ammonia oxidation is inhibited, carbon dioxide is released. The total amount of gas, and thus the pressure, decreases because carbon dioxide is absorbed. From the drop of pressure, the sensor electronics computes and displays the consumed quantity of oxygen.

The main advantages of this method compared to the dilution method are:

Alternative methods

Biosensor

An alternative to measure BOD is the development of biosensors, which are devices for the detection of an analyte that combines a biological component with a physicochemical detector component. Enzymes are the most widely used biological sensing elements in the fabrication of biosensors. Their application in biosensor construction is limited by the tedious, time-consuming and costly enzyme purification methods. Microorganisms provide an ideal alternative to these bottlenecks. [12]

Many micro organisms useful for BOD assessment are relatively easy to maintain in pure cultures, grow and harvest at low cost. Moreover, the use of microbes in the field of biosensors has opened up new possibilities and advantages such as ease of handling, preparation and low cost of device. A number of pure cultures, e.g. Trichosporon cutaneum, Bacillus cereus, Klebsiella oxytoca, Pseudomonas sp. etc. individually, have been used by many workers for the construction of BOD biosensor. On the other hand, many workers have immobilized activated sludge, or a mixture of two or three bacterial species and on various membranes for the construction of BOD biosensor. The most commonly used membranes were polyvinyl alcohol, porous hydrophilic membranes etc. [13]

A defined microbial consortium can be formed by conducting a systematic study, i.e. pre-testing of selected micro-organisms for use as a seeding material in BOD analysis of a wide variety of industrial effluents. Such a formulated consortium can be immobilized on suitable membrane, i.e. charged nylon membrane. Charged nylon membrane is suitable for microbial immobilization, due to the specific binding between negatively charged bacterial cell and positively charged nylon membrane. So the advantages of the nylon membrane over the other membranes are : The dual binding, i.e. Adsorption as well as entrapment, thus resulting in a more stable immobilized membrane. Such specific Microbial consortium based BOD analytical devices, may find great application in monitoring of the degree of pollutant strength, in a wide variety of industrial waste water within a very short time. [13]

Biosensors can be used to indirectly measure BOD via a fast (usually <30 min) to be determined BOD substitute and a corresponding calibration curve method (pioneered by Karube et al., 1977). Consequently, biosensors are now commercially available, but they do have several limitations such as their high maintenance costs, limited run lengths due to the need for reactivation, and the inability to respond to changing quality characteristics as would normally occur in wastewater treatment streams; e.g. diffusion processes of the biodegradable organic matter into the membrane and different responses by different microbial species which lead to problems with the reproducibility of result (Praet et al., 1995). Another important limitation is the uncertainty associated with the calibration function for translating the BOD substitute into the real BOD (Rustum et al., 2008).

Fluorescent

A surrogate to BOD5 has been developed using a resazurin derivative which reveals the extent of oxygen uptake by micro-organisms for organic matter mineralization. [14] A cross-validation performed on 109 samples in Europe and the United-States showed a strict statistical equivalence between results from both methods. [15]

An electrode has been developed based on the luminescence emission of a photo-active chemical compound and the quenching of that emission by oxygen. This quenching photophysics mechanism is described by the Stern–Volmer equation for dissolved oxygen in a solution: [16]

The determination of oxygen concentration by luminescence quenching has a linear response over a broad range of oxygen concentrations and has excellent accuracy and reproducibility. [17]

Polargraphic method

The development of an analytical instrument that utilizes the reduction-oxidation (redox) chemistry of oxygen in the presence of dissimilar metal electrodes was introduced during the 1950s. [18] This redox electrode utilized an oxygen-permeable membrane to allow the diffusion of the gas into an electrochemical cell and its concentration determined by polarographic or galvanic electrodes. This analytical method is sensitive and accurate down to levels of ± 0.1 mg/L dissolved oxygen. Calibration of the redox electrode of this membrane electrode still requires the use of the Henry's law table or the Winkler test for dissolved oxygen.

Software sensor

There have been proposals for automation to make rapid prediction of BOD so it could be used for on-line process monitoring and control. For example, the use of a computerised machine learning method to make rapid inferences about BOD using easy to measure water quality parameters. Ones such as flow rate, chemical oxygen demand, ammonia, nitrogen, pH and suspended solids can be obtained directly and reliably using on-line hardware sensors. In a test of this idea, measurements of these values along with BOD which had been made over three years was used to train and test a model for prediction. The technique could allow for some missing data. It indicated that this approach was possible but needed sufficient historic data to be available. [19]

Real-time BOD monitoring

Until recently, real-time monitoring of BOD was unattainable owing to its complex nature. Recent research by a leading UK university has discovered the link between multiple water quality parameters including electrical conductivity, turbidity, TLF and CDOM. [20] [21] These parameters are all capable of being monitored in real-time through a combination of traditional methods (electrical conductivity via electrodes) and newer methods such as fluorescence. The monitoring of tryptophan-like fluorescence (TLF) has been successfully utilised as a proxy for biological activity and enumeration, particularly with a focus on Escherichia coli (E. Coli). [22] [21] [23] [24] TLF based monitoring is applicable across a wide range of environments, including but by no means limited to sewage treatment works and freshwaters. Therefore, there has been a significant movement towards combined sensor systems that can monitor parameters and use them, in real-time, to provide a reading of BOD that is of laboratory quality.

Dissolved oxygen probes: Membrane and luminescence

The development of an analytical instrument that utilizes the reduction-oxidation (redox) chemistry of oxygen in the presence of dissimilar metal electrodes was introduced during the 1950s. [25] This redox electrode (also known as dissolved oxygen sensor [26] ) utilized an oxygen-permeable membrane to allow the diffusion of the gas into an electrochemical cell and its concentration determined by polarographic or galvanic electrodes. This analytical method is sensitive and accurate to down to levels of ± 0.1 mg/L dissolved oxygen. Calibration of the redox electrode of this membrane electrode still requires the use of the Henry's law table or the Winkler test for dissolved oxygen.

Dissolved oxygen sensor in a sewage treatment plant used as a feedback loop to control the blowers in an aeration system [27]

Test limitations

The test method involves variables limiting reproducibility. Tests normally show observations varying plus or minus ten to twenty percent around the mean. [28] :82

Toxicity

Some wastes contain chemicals capable of suppressing microbiological growth or activity. Potential sources include industrial wastes, antibiotics in pharmaceutical or medical wastes, sanitizers in food processing or commercial cleaning facilities, chlorination disinfection used following conventional sewage treatment, and odor-control formulations used in sanitary waste holding tanks in passenger vehicles or portable toilets. Suppression of the microbial community oxidizing the waste will lower the test result. [28] :85

Appropriate microbial population

The test relies upon a microbial ecosystem with enzymes capable of oxidizing the available organic material. Some waste waters, such as those from biological secondary sewage treatment, will already contain a large population of microorganisms acclimated to the water being tested. An appreciable portion of the waste may be utilized during the holding period prior to commencement of the test procedure. On the other hand, organic wastes from industrial sources may require specialized enzymes. Microbial populations from standard seed sources may take some time to produce those enzymes. A specialized seed culture may be appropriate to reflect conditions of an evolved ecosystem in the receiving waters. [28] :85–87

See also

Related Research Articles

The Winkler test is used to determine the concentration of dissolved oxygen in water samples. Dissolved oxygen (D.O.) is widely used in water quality studies and routine operation of water reclamation facilities to analyze its level of oxygen saturation.

In environmental chemistry, the chemical oxygen demand (COD) is an indicative measure of the amount of oxygen that can be consumed by reactions in a measured solution. It is commonly expressed in mass of oxygen consumed over volume of solution which in SI units is milligrams per litre (mg/L). A COD test can be used to easily quantify the amount of organics in water. The most common application of COD is in quantifying the amount of oxidizable pollutants found in surface water or wastewater. COD is useful in terms of water quality by providing a metric to determine the effect an effluent will have on the receiving body, much like biochemical oxygen demand (BOD).

<span class="mw-page-title-main">Wastewater treatment</span> Converting wastewater into an effluent for return to the water cycle

Wastewater treatment is a process which removes and eliminates contaminants from wastewater and converts this into an effluent that can be returned to the water cycle. Once returned to the water cycle, the effluent creates an acceptable impact on the environment or is reused for various purposes. The treatment process takes place in a wastewater treatment plant. There are several kinds of wastewater which are treated at the appropriate type of wastewater treatment plant. For domestic wastewater, the treatment plant is called a Sewage Treatment. For industrial wastewater, treatment either takes place in a separate Industrial wastewater treatment, or in a sewage treatment plant. Further types of wastewater treatment plants include Agricultural wastewater treatment and leachate treatment plants.

<span class="mw-page-title-main">Activated sludge</span> Wastewater treatment process using aeration and a biological floc

The activated sludgeprocess is a type of biological wastewater treatment process for treating sewage or industrial wastewaters using aeration and a biological floc composed of bacteria and protozoa. It uses air and microorganisms to biologically oxidize organic pollutants, producing a waste sludge containing the oxidized material.

<span class="mw-page-title-main">Industrial wastewater treatment</span> Processes used for treating wastewater that is produced by industries as an undesirable by-product

Industrial wastewater treatment describes the processes used for treating wastewater that is produced by industries as an undesirable by-product. After treatment, the treated industrial wastewater may be reused or released to a sanitary sewer or to a surface water in the environment. Some industrial facilities generate wastewater that can be treated in sewage treatment plants. Most industrial processes, such as petroleum refineries, chemical and petrochemical plants have their own specialized facilities to treat their wastewaters so that the pollutant concentrations in the treated wastewater comply with the regulations regarding disposal of wastewaters into sewers or into rivers, lakes or oceans. This applies to industries that generate wastewater with high concentrations of organic matter, toxic pollutants or nutrients such as ammonia. Some industries install a pre-treatment system to remove some pollutants, and then discharge the partially treated wastewater to the municipal sewer system.

<span class="mw-page-title-main">Septic drain field</span> Type of subsurface wastewater disposal facility

Septic drain fields, also called leach fields or leach drains, are subsurface wastewater disposal facilities used to remove contaminants and impurities from the liquid that emerges after anaerobic digestion in a septic tank. Organic materials in the liquid are catabolized by a microbial ecosystem.

Electrocoagulation (EC) is a technique used for wastewater treatment, wash water treatment, industrially processed water, and medical treatment. Electrocoagulation has become a rapidly growing area of wastewater treatment due to its ability to remove contaminants that are generally more difficult to remove by filtration or chemical treatment systems, such as emulsified oil, total petroleum hydrocarbons, refractory organics, suspended solids, and heavy metals. There are many brands of electrocoagulation devices available, and they can range in complexity from a simple anode and cathode to much more complex devices with control over electrode potentials, passivation, anode consumption, cell REDOX potentials as well as the introduction of ultrasonic sound, ultraviolet light and a range of gases and reactants to achieve so-called Advanced Oxidation Processes for refractory or recalcitrant organic substances.

<span class="mw-page-title-main">Wastewater quality indicators</span> Ways to test the suitability of wastewater

Wastewater quality indicators are laboratory test methodologies to assess suitability of wastewater for disposal, treatment or reuse. The main parameters in sewage that are measured to assess the sewage strength or quality as well as treatment options include: solids, indicators of organic matter, nitrogen, phosphorus, indicators of fecal contamination. Tests selected vary with the intended use or discharge location. Tests can measure physical, chemical, and biological characteristics of the wastewater. Physical characteristics include temperature and solids. Chemical characteristics include pH value, dissolved oxygen concentrations, biochemical oxygen demand (BOD) and chemical oxygen demand (COD), nitrogen, phosphorus, chlorine. Biological characteristics are determined with bioassays and aquatic toxicology tests.

<span class="mw-page-title-main">Secondary treatment</span> Biological treatment process for wastewater or sewage

Secondary treatment is the removal of biodegradable organic matter from sewage or similar kinds of wastewater. The aim is to achieve a certain degree of effluent quality in a sewage treatment plant suitable for the intended disposal or reuse option. A "primary treatment" step often precedes secondary treatment, whereby physical phase separation is used to remove settleable solids. During secondary treatment, biological processes are used to remove dissolved and suspended organic matter measured as biochemical oxygen demand (BOD). These processes are performed by microorganisms in a managed aerobic or anaerobic process depending on the treatment technology. Bacteria and protozoa consume biodegradable soluble organic contaminants while reproducing to form cells of biological solids. Secondary treatment is widely used in sewage treatment and is also applicable to many agricultural and industrial wastewaters.

Microbial fuel cell (MFC) is a type of bioelectrochemical fuel cell system also known as micro fuel cell that generates electric current by diverting electrons produced from the microbial oxidation of reduced compounds on the anode to oxidized compounds such as oxygen on the cathode through an external electrical circuit. MFCs produce electricity by using the electrons derived from biochemical reactions catalyzed by bacteria.Comprehensive Biotechnology MFCs can be grouped into two general categories: mediated and unmediated. The first MFCs, demonstrated in the early 20th century, used a mediator: a chemical that transfers electrons from the bacteria in the cell to the anode. Unmediated MFCs emerged in the 1970s; in this type of MFC the bacteria typically have electrochemically active redox proteins such as cytochromes on their outer membrane that can transfer electrons directly to the anode. In the 21st century MFCs have started to find commercial use in wastewater treatment.

An aerobic treatment system (ATS), often called an aerobic septic system, is a small scale sewage treatment system similar to a septic tank system, but which uses an aerobic process for digestion rather than just the anaerobic process used in septic systems. These systems are commonly found in rural areas where public sewers are not available, and may be used for a single residence or for a small group of homes.

Coffee wastewater, also known as coffee effluent, is a byproduct of coffee processing. Its treatment and disposal is an important environmental consideration for coffee processing as wastewater is a form of industrial water pollution.

<span class="mw-page-title-main">Sewage treatment</span> Process of removing contaminants from municipal wastewater

Sewage treatment is a type of wastewater treatment which aims to remove contaminants from sewage to produce an effluent that is suitable to discharge to the surrounding environment or an intended reuse application, thereby preventing water pollution from raw sewage discharges. Sewage contains wastewater from households and businesses and possibly pre-treated industrial wastewater. There are a high number of sewage treatment processes to choose from. These can range from decentralized systems to large centralized systems involving a network of pipes and pump stations which convey the sewage to a treatment plant. For cities that have a combined sewer, the sewers will also carry urban runoff (stormwater) to the sewage treatment plant. Sewage treatment often involves two main stages, called primary and secondary treatment, while advanced treatment also incorporates a tertiary treatment stage with polishing processes and nutrient removal. Secondary treatment can reduce organic matter from sewage,  using aerobic or anaerobic biological processes. A so-called quarternary treatment step can also be added for the removal of organic micropollutants, such as pharmaceuticals. This has been implemented in full-scale for example in Sweden.

<span class="mw-page-title-main">Sewage</span> Wastewater that is produced by a community of people

Sewage is a type of wastewater that is produced by a community of people. It is typically transported through a sewer system. Sewage consists of wastewater discharged from residences and from commercial, institutional and public facilities that exist in the locality. Sub-types of sewage are greywater and blackwater. Sewage also contains soaps and detergents. Food waste may be present from dishwashing, and food quantities may be increased where garbage disposal units are used. In regions where toilet paper is used rather than bidets, that paper is also added to the sewage. Sewage contains macro-pollutants and micro-pollutants, and may also incorporate some municipal solid waste and pollutants from industrial wastewater.

<span class="mw-page-title-main">Facultative lagoon</span>

Facultative lagoons are a type of waste stabilization pond used for biological treatment of industrial and domestic wastewater. Sewage or organic waste from food or fiber processing may be catabolized in a system of constructed ponds where adequate space is available to provide an average waste retention time exceeding a month. A series of ponds prevents mixing of untreated waste with treated wastewater and allows better control of waste residence time for uniform treatment efficiency.

<span class="mw-page-title-main">Rotating biological contactor</span> Biological process for wastewater treatment

A rotating biological contactor or RBC is a biological fixed-film treatment process used in the secondary treatment of wastewater following primary treatment. The primary treatment process involves removal of grit, sand and coarse suspended material through a screening process, followed by settling of suspended solids. The RBC process allows the wastewater to come in contact with a biological film in order to remove pollutants in the wastewater before discharge of the treated wastewater to the environment, usually a body of water. A rotating biological contactor is a type of secondary (biological) treatment process. It consists of a series of closely spaced, parallel discs mounted on a rotating shaft which is supported just above the surface of the wastewater. Microorganisms grow on the surface of the discs where biological degradation of the wastewater pollutants takes place.

Mixed liquor suspended solids (MLSS) is the concentration of suspended solids, in an aeration tank during the activated sludge process, which occurs during the treatment of waste water. The units MLSS is primarily measured in milligram per litre (mg/L), but for activated sludge its mostly measured in gram per litre [g/L] which is equal to kilogram per cubic metre [kg/m3]. Mixed liquor is a combination of raw or unsettled wastewater or pre-settled wastewater and activated sludge within an aeration tank. MLSS consists mostly of microorganisms and non-biodegradable suspended matter. MLSS is an important part of the activated sludge process to ensure that there is a sufficient quantity of active biomass available to consume the applied quantity of organic pollutant at any time. This is known as the food to microorganism ratio, more commonly notated as the F/M ratio. By maintaining this ratio at the appropriate level the biomass will consume high percentages of the food. This minimizes the loss of residual food in the treated effluent. In simple terms, the more the biomass consumes the lower the biochemical oxygen demand (BOD) will be in the discharge. It is important that MLSS removes COD and BOD in order to purify water for clean surface waters, and subsequently clean drinking water and hygiene. Raw sewage enters in the water treatment process with a concentration of sometimes several hundred mg/L of BOD. Upon being treated by screening, pre-settling, activated sludge processes or other methods of treatment, the concentration of BOD in water can be lowered to less than 2 mg/L, which is considered to be clean, safe to discharge to surface waters or to reuse water.

<span class="mw-page-title-main">Vermifilter</span> Aerobic treatment system, consisting of a biological reactor containing media

A vermifilter is an aerobic treatment system, consisting of a biological reactor containing media that filters organic material from wastewater. The media also provides a habitat for aerobic bacteria and composting earthworms that purify the wastewater by removing pathogens and oxygen demand. The "trickling action" of the wastewater through the media dissolves oxygen into the wastewater, ensuring the treatment environment is aerobic for rapid decomposition of organic substances.

<span class="mw-page-title-main">BOD bottle</span>

BOD Bottle or an incubation bottle is a main apparatus used for the Biological Oxygen Demand (BOD) test. During the five-day BOD or BOD5 test process, the BOD bottle is used for incubating diluted samples under the 20 °C or 68 °F of temperature.

Microbial electrochemical technologies (METs) use microorganisms as electrochemical catalyst, merging the microbial metabolism with electrochemical processes for the production of bioelectricity, biofuels, H2 and other valuable chemicals. Microbial fuel cells (MFC) and microbial electrolysis cells (MEC) are prominent examples of METs. While MFC is used to generate electricity from organic matter typically associated with wastewater treatment, MEC use electricity to drive chemical reactions such as the production of H2 or methane. Recently, microbial electrosynthesis cells (MES) have also emerged as a promising MET, where valuable chemicals can be produced in the cathode compartment. Other MET applications include microbial remediation cell, microbial desalination cell, microbial solar cell, microbial chemical cell, etc.,.

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Further reading