Cytochrome c oxidase subunit I

"Cox1" redirects here. Particularly in a medical context, this can also refer to cyclooxygenase-1.

COX1
Identifiers
Aliases	COX1 , mitochondrially encoded cytochrome c oxidase I, COI, MTCO1, Main subunit of cytochrome c oxidase, CO I, cytochrome c oxidase subunit I
External IDs	OMIM: 516030 MGI: 102504 HomoloGene: 5016 GeneCards: COX1
Gene location (Human) Chr. Mitochondrial DNA (human) [1] Band n/a Start 5,904 bp [1] End 7,445 bp [1]
Gene location (Mouse) Chr. Mitochondrial DNA (mouse) [2] Band n/a Start 5,328 bp [2] End 6,872 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right uterine tube duodenum stromal cell of endometrium right coronary artery rectum Brodmann area 9 smooth muscle tissue gallbladder appendix right lung Top expressed in cerebellar cortex esophagus superior frontal gyrus hippocampus proper jejunum ganglionic eminence quadriceps femoris muscle kidney lip colon More reference expression data BioGPS n/a
Gene ontology Molecular function cytochrome-c oxidase activity metal ion binding protein binding heme binding oxidoreductase activity Cellular component integral component of membrane membrane mitochondrion mitochondrial inner membrane respirasome respiratory chain complex IV mitochondrial respiratory chain complex III mitochondrial respiratory chain complex IV Biological process response to copper ion human ageing response to electrical stimulus response to oxidative stress cerebellum development oxidative phosphorylation mitochondrial electron transport, cytochrome c to oxygen aerobic respiration electron transport coupled proton transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4512 17708 Ensembl ENSG00000198804 ENSMUSG00000064351 UniProt P00395 P00397 RefSeq (mRNA) n/a n/a RefSeq (protein) n/a NP_904330 Location (UCSC) Chr M: 0.01 – 0.01 Mb Chr M: 0.01 – 0.01 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Cytochrome c oxidase subunit I
Structure of the 13-subunit oxidized cytochrome c oxidase. [5]
Identifiers
Symbol	COX1 or COI
Pfam	PF00115
InterPro	IPR000883
PROSITE	PDOC00074
SCOP2	1occ / SCOPe / SUPFAM
TCDB	3.D.4
OPM superfamily	4
OPM protein	1v55
CDD	cd01663
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Location of the MT-CO1 gene in the human mitochondrial genome. MT-CO1 is one of the three cytochrome c oxidase subunit mitochondrial genes (orange boxes).

Cytochrome c oxidase I (COX1) also known as mitochondrially encoded cytochrome c oxidase I (MT-CO1) is a protein that is encoded by the MT-CO1 gene in eukaryotes. ^[6] The gene is also called COX1, CO1, or COI. ^[7] Cytochrome c oxidase I is the main subunit of the cytochrome c oxidase complex. In humans, mutations in MT-CO1 have been associated with Leber's hereditary optic neuropathy (LHON), acquired idiopathic sideroblastic anemia, Complex IV deficiency, colorectal cancer, sensorineural deafness, and recurrent myoglobinuria. ^{[8] [9] [10]}

Structure

In humans, the MT-CO1 gene is located from nucleotide pairs 5904 to 7444 on the guanine-rich heavy (H) section of mtDNA. The gene product is a 57 kDa protein composed of 513 amino acids. ^{[11] [12]}

Function

Cytochrome c oxidase subunit I (CO1 or MT-CO1) is one of three mitochondrial DNA (mtDNA) encoded subunits (MT-CO1, MT-CO2, MT-CO3) of respiratory complex IV. Complex IV is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. ^[6]

Cytochrome c oxidase (EC 1.9.3.1) is a key enzyme in aerobic metabolism. Proton pumping heme-copper oxidases represent the terminal, energy-transfer enzymes of respiratory chains in prokaryotes and eukaryotes. The CuB-heme a3 (or heme o) binuclear centre, associated with the largest subunit I of cytochrome c and ubiquinol oxidases (EC 1.10.3.10), is directly involved in the coupling between dioxygen reduction and proton pumping. ^{[13] [14]} Some terminal oxidases generate a transmembrane proton gradient across the plasma membrane (prokaryotes) or the mitochondrial inner membrane (eukaryotes).

The enzyme complex consists of 3-4 subunits (prokaryotes) up to 13 polypeptides (mammals) of which only the catalytic subunit (equivalent to mammalian subunit I (COI)) is found in all heme-copper respiratory oxidases. The presence of a bimetallic centre (formed by a high-spin heme and copper B) as well as a low-spin heme, both ligated to six conserved histidine residues near the outer side of four transmembrane spans within COI is common to all family members. ^{[15] [16] [17]} In contrast to eukaryotes the respiratory chain of prokaryotes is branched to multiple terminal oxidases. The enzyme complexes vary in heme and copper composition, substrate type and substrate affinity. The different respiratory oxidases allow the cells to customize their respiratory systems according to a variety of environmental growth conditions. ^[13]

It has been shown that eubacterial quinol oxidase was derived from cytochrome c oxidase in Gram-positive bacteria and that archaebacterial quinol oxidase has an independent origin. A considerable amount of evidence suggests that Pseudomonadota (also known as proteobacteria or purple bacteria) acquired quinol oxidase through a lateral gene transfer from Gram-positive bacteria. ^[13]

A related nitric-oxide reductase (EC 1.7.99.7) exists in denitrifying species of archaea and eubacteria and is a heterodimer of cytochromes b and c. Phenazine methosulphate can act as acceptor. It has been suggested that cytochrome c oxidase catalytic subunits evolved from ancient nitric oxide reductases that could reduce both nitrogen and oxygen. ^{[18] [19]}

Clinical significance

Mutations in this gene are associated with Leber's hereditary optic neuropathy (LHON), acquired idiopathic sideroblastic anemia, Complex IV deficiency, colorectal cancer, sensorineural deafness, and recurrent myoglobinuria. ^{[8] [9] [10]}

Leber's hereditary optic neuropathy (LHON)

LHON, correlated with mutations in MT-CO1, is characterized by optic nerve dysfunction, causing subacute or acute central vision loss. Some patients may display neurological or cardiac conduction defects. Because this disease is a result of mitochondrial DNA mutations affecting the respiratory chain complexes, it is inherited maternally. ^{[20] [9] [10]}

Acquired Idiopathic Sideroblastic Anemia

MT-CO1 may be involved in the development of acquired idiopathic sideroblastic anemia. Mutations in mitochondrial DNA can cause respiratory chain dysfunction, preventing reduction of ferric iron to ferrous iron, which is required for the final step in mitochondrial biosynthesis of heme. The result is a ferric accumulation in mitochondria and insufficient heme production. ^{[21] [22] [9] [10]}

Mitochondrial Complex IV deficiency (MT-C4D)

Mutations in this gene can cause mitochondrial Complex IV deficiency, a disease of the mitochondrial respiratory chain displaying a wide variety of clinical manifestations ranging from isolated myopathy to a severe multisystem disease affecting multiple organs and tissues. Symptoms may include liver dysfunction and hepatomegaly, hypotonia, muscle weakness, exercise intolerance, delayed motor development, mental retardation, developmental delay, and hypertrophic cardiomyopathy. In some patients, the hypertrophic cardiomyopathy is fatal at the neonatal stage. Other affected individuals may manifest Leigh disease. ^{[23] [24] [9] [10]}

Colorectal cancer (CRC)

MT-CO1 mutations play a role in colorectal cancer, a very complex disease displaying malignant lesions in the inner walls of the colon and rectum. Numerous such genetic alterations are often involved with the progression of adenoma, or premalignant lesions, to invasive adenocarcinoma. Long-standing ulcerative colitis, colon polyps, and family history are risk factors for colorectal cancer. ^{[25] [26] [9] [10]}

Recurrent myoglobinuria mitochondrial (RM-MT)

RM-MT is a disease that is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, exercise intolerance, low muscle capacity for oxidative phosphorylation, and followed by excretion of myoglobin in the urine. It has been associated with mitochondrial myopathy. A G5920A mutation, and a heteroplasmic G6708A nonsense mutation have been associated with COX deficiency and RM-MT. ^{[27] [28] [9] [10]}

Deafness, sensorineural, mitochondrial (DFNM)

DFNM is a form of non-syndromic deafness with maternal inheritance. Affected individuals manifest progressive, postlingual, sensorineural hearing loss involving high frequencies. The mutation, A1555G, has been associated with this disease. ^{[29] [9] [10]}

Subfamilies

• Cytochrome c oxidase cbb3-type, subunit I InterPro :  IPR004677
• Cytochrome o ubiquinol oxidase, subunit I InterPro :  IPR014207
• Cytochrome aa3 quinol oxidase, subunit I InterPro :  IPR014233
• Cytochrome c oxidase, subunit I bacterial type InterPro :  IPR014241

Use in DNA barcoding

MT-CO1 is a gene that is often used as a DNA barcode to identify animal species. The MT-CO1 gene sequence is suitable for this role because its mutation rate is generally fast enough to distinguish closely related species and also because its sequence is conserved among conspecifics. Contrary to the primary objection raised by skeptics that MT-CO1 sequence differences are too small to be detected between closely related species, more than 2% sequence divergence is typically detected between closely related animal species, ^[30] suggesting that the barcode is effective for most animals. In most if not all seed plants, however, the rate of evolution of MT-CO1 is very slow. It has also been suggested that MT-CO1 may be a better gene for DNA barcoding of soil fungi than ITS (the gene most commonly used for mycological barcoding). ^[31]

MT-COI (= CCOI) in colonic crypts

Colonic crypts (intestinal glands) within four tissue sections. The cells have been stained by immunohistochemistry to show a brown-orange color if the cells produce the mitochondrial protein cytochrome c oxidase subunit I (CCOI, synonym for MT-COI), and the nuclei of the cells (located at the outer edges of the cells lining the walls of the crypts) are stained blue-gray with haematoxylin. Panels A, B were cut across the long axes of the crypts and panels C, D were cut parallel to the long axes of the crypts. In panel A the bar shows 100 µm and allows an estimate of the frequency of crypts in the colonic epithelium. Panel B includes three crypts in cross-section, each with one segment deficient for MT-COI expression and at least one crypt, on the right side, undergoing fission into two crypts. Panel C shows, on the left side, a crypt fissioning into two crypts. Panel D shows typical small clusters of two and three MT-COI deficient crypts (the bar shows 50 µm). The images were made from original photomicrographs, but panels A, B and D were also included in an article and illustrations were published with Creative Commons Attribution-Noncommercial License allowing re-use.

The MT-COI protein, also known as CCOI, is usually expressed at a high level in the cytoplasm of colonic crypts of the human large intestine (colon). However, MT-COI is frequently lost in colonic crypts with age in humans and is also often absent in field defects that give rise to colon cancers as well as in portions of colon cancers. ^[32]

The epithelial inner surface of the colon is punctuated by invaginations, the colonic crypts. The colon crypts are shaped like microscopic thick walled test tubes with a central hole down the length of the tube (the crypt lumen). Four tissue sections are shown in the image in this section, two cut across the long axes of the crypts and two cut parallel to the long axes.

Most of the human colonic crypts in the images have high expression of the brown-orange stained MT-COI. However, in some of the colonic crypts all of the cells lack MT-COI and appear mostly white, with their main color being the blue-gray staining of the nuclei at the outer walls of the crypts. Greaves et al. ^[33] showed that deficiencies of MT-COI in colonic crypts are due to mutations in the MT-COI gene. As seen in panel B, a portion of the stem cells of three crypts appear to have a mutation in MT-COI, so that 40% to 50% of the cells arising from those stem cells form a white segment in the cross-cut area.

In humans, the percent of colonic crypts deficient for MT-COI is less than 1% before age 40, but then increases linearly with age. ^[32] On average, the percent of colonic crypts deficient for MT-COI reaches 18% in women and 23% in men by 80–84 years of age. ^[32] Colonic tumors often arise in a field of crypts containing a large cluster (as many as 410) of MT-COI-deficient crypts. In colonic cancers, up to 80% of tumor cells can be deficient in MT-COI. ^[32]

As seen in panels C and D, crypts are about 75 to about 110 cells long. The average crypt circumference is 23 cells. ^[34] Based on these measurements, crypts have between 1725 and 2530 cells. Another report gave a range of 1500 to 4900 cells per colonic crypt. ^[35]

The occurrence of frequent crypts with almost complete loss of MT-COI in their 1700 to 5,000 cells suggests a process of natural selection. However, it has also been shown that a deficiency throughout a particular crypt due to an initial mitochondrial DNA mutation may occasionally occur through a stochastic process. ^{[36] [37]} Nevertheless, the frequent occurrence of MT-COI deficiency in many crypts within a colon epithelium indicates that absence of MT-COI likely provides a selective advantage.

MT-COI is coded for by the mitochondrial chromosome. There are multiple copies of the chromosome in most mitochondria, usually between 2 and 6 per mitochondrion. ^{[38] [39] [40]} If a mutation occurs in MT-COI in one chromosome of a mitochondrion, there may be random segregation of the chromosomes during mitochondrial fission to generate new mitochondria. This can give rise to a mitochondrion with primarily or solely MT-COI-mutated chromosomes.

A mitochondrion with largely MT-COI-mutated chromosomes would need to have a positive selection bias in order to frequently become the main type of mitochondrion in a cell (a cell with MT-COI-deficient homoplasmy). There are about 100 to 700 mitochondria per cell, depending on cell type. ^{[39] [40]} Furthermore, there is fairly rapid turnover of mitochondria, so that a mitochondrion with MT-COI-mutated chromosomes and a positive selection bias could shortly become the major type of mitochondrion in a cell. The average half-life of mitochondria in rats, depending on cell type, is between 9 and 24 days, ^[41] and in mice is about 2 days. ^[42] In humans it is likely that the half life of mitochondria is also a matter of days to weeks.

A stem cell at the base of a colonic crypt that was largely MT-COI-deficient may compete with the other 4 or 5 stem cells to take over the stem cell niche. If this occurs, then the colonic crypt would be deficient in MT-COI in all 1700 to 5,000 cells, as is indicated for some crypts in panels A, B and D of the image.

Crypts of the colon can reproduce by fission, as seen in panel C, where a crypt is fissioning to form two crypts, and in panel B where at least one crypt appears to be fissioning. Most crypts deficient in MT-COI are in clusters of crypts (clones of crypts) with two or more MT-COI-deficient crypts adjacent to each other (see panel D). ^[32] This illustrates that clones of deficient crypts often arise, and thus that there is likely a positive selective bias that has allowed them to spread in the human colonic epithelium.

It is not clear why a deficiency of MT-COI should have a positive selective bias. One suggestion ^[32] is that deficiency of MT-COI in a mitochondrion leads to lower reactive oxygen production (and less oxidative damage) and this provides a selective advantage in competition with other mitochondria within the same cell to generate homoplasmy for MT-COI-deficiency. Another suggestion was that cells with a deficiency in cytochrome c oxidase are apoptosis resistant, and thus more likely to survive. The linkage of MT-COI to apoptosis arises because active cytochrome c oxidase oxidizes cytochrome c, which then activates pro-caspase 9, leading to apoptosis. ^[43] These two factors may contribute to the frequent occurrence of MT-COI-deficient colonic crypts with age or during carcinogenesis in the human colon.

Interactions

Within the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase) complex, the encoded protein interacts with COA3 and SMIM20/MITRAC7. This interaction with SMIM20 stabilizes the newly synthesized MT-CO1 and prevents its premature turnover. ^[44] Additionally, it interacts with TMEM177 in a COX20-dependent manner. ^{[45] [9] [10]}

References

1. GRCh38: Ensembl release 89: ENSG00000198804 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000064351 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Tsukihara T, Aoyama H, Yamashita E, Tomizaki T, Yamaguchi H, Shinzawa-Itoh K, et al. (May 1996). "The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A". Science. 272 (5265): 1136–1144. Bibcode:1996Sci...272.1136T. doi:10.1126/science.272.5265.1136. PMID   8638158. S2CID   20860573.
6. "Entrez Gene: Cytochrome c oxidase subunit I".
7. Kosakyan A, Heger TJ, Leander BS, Todorov M, Mitchell EA, Lara E (May 2012). "COI barcoding of Nebelid testate amoebae (Amoebozoa: Arcellinida): extensive cryptic diversity and redefinition of the Hyalospheniidae Schultze" (PDF). Protist. 163 (3): 415–434. doi:10.1016/j.protis.2011.10.003. PMID   22130576.
8. Online Mendelian Inheritance in Man (OMIM): 516030
9. "MT-CO1 - Cytochrome c oxidase subunit 1 - Homo sapiens (Human) - MT-CO1 gene & protein". www.uniprot.org. Retrieved 2018-08-13.  This article incorporates text available under the CC BY 4.0 license.
10. "UniProt: the universal protein knowledgebase". Nucleic Acids Research. 45 (D1): D158–D169. January 2017. doi:10.1093/nar/gkw1099. PMC   5210571 . PMID   27899622.
11. Zong NC, Li H, Li H, Lam MP, Jimenez RC, Kim CS, et al. (October 2013). "Integration of cardiac proteome biology and medicine by a specialized knowledgebase". Circulation Research. 113 (9): 1043–1053. doi:10.1161/CIRCRESAHA.113.301151. PMC   4076475 . PMID   23965338.
12. "MT-CO1 - Cytochrome c oxidase subunit 1". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB).^{[ permanent dead link ]}
13. García-Horsman JA, Barquera B, Rumbley J, Ma J, Gennis RB (September 1994). "The superfamily of heme-copper respiratory oxidases". Journal of Bacteriology. 176 (18): 5587–5600. doi:10.1128/jb.176.18.5587-5600.1994. PMC   196760 . PMID   8083153.
14. Papa S, Capitanio N, Glaser P, Villani G (May 1994). "The proton pump of heme-copper oxidases". Cell Biology International. 18 (5): 345–355. doi:10.1006/cbir.1994.1084. PMID   8049679. S2CID   36428993.
15. Castresana J, Lübben M, Saraste M, Higgins DG (June 1994). "Evolution of cytochrome oxidase, an enzyme older than atmospheric oxygen". The EMBO Journal. 13 (11): 2516–2525. doi:10.1002/j.1460-2075.1994.tb06541.x. PMC   395125 . PMID   8013452.
16. Capaldi RA, Malatesta F, Darley-Usmar VM (July 1983). "Structure of cytochrome c oxidase". Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics. 726 (2): 135–148. doi:10.1016/0304-4173(83)90003-4. PMID   6307356.
17. Holm L, Saraste M, Wikström M (September 1987). "Structural models of the redox centres in cytochrome oxidase". The EMBO Journal. 6 (9): 2819–2823. doi:10.1002/j.1460-2075.1987.tb02578.x. PMC   553708 . PMID   2824194.
18. Saraste M, Castresana J (March 1994). "Cytochrome oxidase evolved by tinkering with denitrification enzymes". FEBS Letters. 341 (1): 1–4. doi: 10.1016/0014-5793(94)80228-9 . PMID   8137905. S2CID   1248917.
19. Chen J, Strous M (February 2013). "Denitrification and aerobic respiration, hybrid electron transport chains and co-evolution". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1827 (2): 136–144. doi: 10.1016/j.bbabio.2012.10.002 . PMID   23044391.
20. Brown MD, Yang CC, Trounce I, Torroni A, Lott MT, Wallace DC (August 1992). "A mitochondrial DNA variant, identified in Leber hereditary optic neuropathy patients, which extends the amino acid sequence of cytochrome c oxidase subunit I". American Journal of Human Genetics. 51 (2): 378–385. PMC   1682694 . PMID   1322638.
21. Gattermann N, Retzlaff S, Wang YL, Hofhaus G, Heinisch J, Aul C, Schneider W (December 1997). "Heteroplasmic point mutations of mitochondrial DNA affecting subunit I of cytochrome c oxidase in two patients with acquired idiopathic sideroblastic anemia". Blood. 90 (12): 4961–4972. doi: 10.1182/blood.V90.12.4961 . PMID   9389715.
22. Bröker S, Meunier B, Rich P, Gattermann N, Hofhaus G (November 1998). "MtDNA mutations associated with sideroblastic anaemia cause a defect of mitochondrial cytochrome c oxidase". European Journal of Biochemistry. 258 (1): 132–138. doi:10.1046/j.1432-1327.1998.2580132.x. PMID   9851701.
23. Varlamov DA, Kudin AP, Vielhaber S, Schröder R, Sassen R, Becker A, et al. (August 2002). "Metabolic consequences of a novel missense mutation of the mtDNA CO I gene". Human Molecular Genetics. 11 (16): 1797–1805. doi: 10.1093/hmg/11.16.1797 . PMID   12140182.
24. Lucioli S, Hoffmeier K, Carrozzo R, Tessa A, Ludwig B, Santorelli FM (March 2006). "Introducing a novel human mtDNA mutation into the Paracoccus denitrificans COX I gene explains functional deficits in a patient". Neurogenetics. 7 (1): 51–57. doi:10.1007/s10048-005-0015-z. PMID   16284789. S2CID   21304246.
25. Greaves LC, Preston SL, Tadrous PJ, Taylor RW, Barron MJ, Oukrif D, et al. (January 2006). "Mitochondrial DNA mutations are established in human colonic stem cells, and mutated clones expand by crypt fission". Proceedings of the National Academy of Sciences of the United States of America. 103 (3): 714–719. Bibcode:2006PNAS..103..714G. doi: 10.1073/pnas.0505903103 . PMC   1325106 . PMID   16407113.
26. Namslauer I, Brzezinski P (March 2009). "A mitochondrial DNA mutation linked to colon cancer results in proton leaks in cytochrome c oxidase". Proceedings of the National Academy of Sciences of the United States of America. 106 (9): 3402–3407. Bibcode:2009PNAS..106.3402N. doi: 10.1073/pnas.0811450106 . PMC   2651238 . PMID   19218458.
27. Karadimas CL, Greenstein P, Sue CM, Joseph JT, Tanji K, Haller RG, et al. (September 2000). "Recurrent myoglobinuria due to a nonsense mutation in the COX I gene of mitochondrial DNA". Neurology. 55 (5): 644–649. doi:10.1212/wnl.55.5.644. PMID   10980727. S2CID   26776388.
28. Kollberg G, Moslemi AR, Lindberg C, Holme E, Oldfors A (February 2005). "Mitochondrial myopathy and rhabdomyolysis associated with a novel nonsense mutation in the gene encoding cytochrome c oxidase subunit I". Journal of Neuropathology and Experimental Neurology. 64 (2): 123–128. doi: 10.1093/jnen/64.2.123 . PMID   15751226.
29. Pandya A, Xia XJ, Erdenetungalag R, Amendola M, Landa B, Radnaabazar J, et al. (December 1999). "Heterogenous point mutations in the mitochondrial tRNA Ser(UCN) precursor coexisting with the A1555G mutation in deaf students from Mongolia". American Journal of Human Genetics. 65 (6): 1803–1806. doi:10.1086/302658. PMC   1288397 . PMID   10577941.
30. Hebert PD, Ratnasingham S, deWaard JR (August 2003). "Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species". Proceedings. Biological Sciences. 270 Suppl 1 (Suppl 1): S96–S99. doi:10.1098/rsbl.2003.0025. PMC   1698023 . PMID   12952648.
31. Molitor C, Inthavong B, Sage L, Geremia RA, Mouhamadou B (January 2010). "Potentiality of the cox1 gene in the taxonomic resolution of soil fungi". FEMS Microbiology Letters. 302 (1): 76–84. doi: 10.1111/j.1574-6968.2009.01839.x . PMID   19909345.
32. Bernstein C, Facista A, Nguyen H, Zaitlin B, Hassounah N, Loustaunau C, et al. (December 2010). "Cancer and age related colonic crypt deficiencies in cytochrome c oxidase I". World Journal of Gastrointestinal Oncology. 2 (12): 429–442. doi:10.4251/wjgo.v2.i12.429. PMC   3011097 . PMID   21191537.
33. Greaves LC, Preston SL, Tadrous PJ, Taylor RW, Barron MJ, Oukrif D, et al. (January 2006). "Mitochondrial DNA mutations are established in human colonic stem cells, and mutated clones expand by crypt fission". Proceedings of the National Academy of Sciences of the United States of America. 103 (3): 714–719. Bibcode:2006PNAS..103..714G. doi: 10.1073/pnas.0505903103 . PMC   1325106 . PMID   16407113.
34. Baker AM, Cereser B, Melton S, Fletcher AG, Rodriguez-Justo M, Tadrous PJ, et al. (August 2014). "Quantification of crypt and stem cell evolution in the normal and neoplastic human colon". Cell Reports. 8 (4): 940–947. doi:10.1016/j.celrep.2014.07.019. PMC   4471679 . PMID   25127143.
35. Nooteboom M, Johnson R, Taylor RW, Wright NA, Lightowlers RN, Kirkwood TB, et al. (February 2010). "Age-associated mitochondrial DNA mutations lead to small but significant changes in cell proliferation and apoptosis in human colonic crypts". Aging Cell. 9 (1): 96–99. doi:10.1111/j.1474-9726.2009.00531.x. PMC   2816353 . PMID   19878146.
36. Coller HA, Bodyak ND, Khrapko K (April 2002). "Frequent intracellular clonal expansions of somatic mtDNA mutations: significance and mechanisms". Annals of the New York Academy of Sciences. 959 (1): 434–447. Bibcode:2002NYASA.959..434C. doi:10.1111/j.1749-6632.2002.tb02113.x. PMID   11976216. S2CID   40639679.
37. Nekhaeva E, Bodyak ND, Kraytsberg Y, McGrath SB, Van Orsouw NJ, Pluzhnikov A, et al. (April 2002). "Clonally expanded mtDNA point mutations are abundant in individual cells of human tissues". Proceedings of the National Academy of Sciences of the United States of America. 99 (8): 5521–5526. Bibcode:2002PNAS...99.5521N. doi: 10.1073/pnas.072670199 . PMC   122802 . PMID   11943860.
38. Legros F, Malka F, Frachon P, Lombès A, Rojo M (June 2004). "Organization and dynamics of human mitochondrial DNA". Journal of Cell Science. 117 (Pt 13): 2653–2662. doi:10.1242/jcs.01134. PMID   15138283.
39. Robin ED, Wong R (September 1988). "Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells". Journal of Cellular Physiology. 136 (3): 507–513. doi:10.1002/jcp.1041360316. PMID   3170646. S2CID   2841036.
40. Satoh M, Kuroiwa T (September 1991). "Organization of multiple nucleoids and DNA molecules in mitochondria of a human cell". Experimental Cell Research. 196 (1): 137–140. doi:10.1016/0014-4827(91)90467-9. PMID   1715276.
41. Menzies RA, Gold PH (April 1971). "The turnover of mitochondria in a variety of tissues of young adult and aged rats". The Journal of Biological Chemistry. 246 (8): 2425–2429. doi: 10.1016/S0021-9258(18)62305-1 . PMID   5553400.
42. Miwa S, Lawless C, von Zglinicki T (December 2008). "Mitochondrial turnover in liver is fast in vivo and is accelerated by dietary restriction: application of a simple dynamic model". Aging Cell. 7 (6): 920–923. doi:10.1111/j.1474-9726.2008.00426.x. PMC   2659384 . PMID   18691181.
43. Brown GC, Borutaite V (2008). "Regulation of apoptosis by the redox state of cytochrome c". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1777 (7–8): 877–881. doi: 10.1016/j.bbabio.2008.03.024 . PMID   18439415.
44. Dennerlein S, Oeljeklaus S, Jans D, Hellwig C, Bareth B, Jakobs S, et al. (September 2015). "MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly". Cell Reports. 12 (10): 1644–1655. doi: 10.1016/j.celrep.2015.08.009 . PMID   26321642.
45. Lorenzi I, Oeljeklaus S, Aich A, Ronsör C, Callegari S, Dudek J, et al. (February 2018). "The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1865 (2): 323–333. doi:10.1016/j.bbamcr.2017.11.010. PMC   5764226 . PMID   29154948.

Further reading

• Torroni A, Achilli A, Macaulay V, Richards M, Bandelt HJ (June 2006). "Harvesting the fruit of the human mtDNA tree". Trends in Genetics. 22 (6): 339–345. doi:10.1016/j.tig.2006.04.001. PMID   16678300.
• Bodenteich A, Mitchell LG, Polymeropoulos MH, Merril CR (May 1992). "Dinucleotide repeat in the human mitochondrial D-loop". Human Molecular Genetics. 1 (2): 140. doi:10.1093/hmg/1.2.140-a. PMID   1301157.
• Brown MD, Yang CC, Trounce I, Torroni A, Lott MT, Wallace DC (August 1992). "A mitochondrial DNA variant, identified in Leber hereditary optic neuropathy patients, which extends the amino acid sequence of cytochrome c oxidase subunit I". American Journal of Human Genetics. 51 (2): 378–385. PMC   1682694 . PMID   1322638.
• Lu X, Walker T, MacManus JP, Seligy VL (July 1992). "Differentiation of HT-29 human colonic adenocarcinoma cells correlates with increased expression of mitochondrial RNA: effects of trehalose on cell growth and maturation". Cancer Research. 52 (13): 3718–3725. PMID   1377597.
• Marzuki S, Noer AS, Lertrit P, Thyagarajan D, Kapsa R, Utthanaphol P, Byrne E (December 1991). "Normal variants of human mitochondrial DNA and translation products: the building of a reference data base". Human Genetics. 88 (2): 139–145. doi:10.1007/bf00206061. PMID   1757091. S2CID   28048453.
• Moraes CT, Andreetta F, Bonilla E, Shanske S, DiMauro S, Schon EA (March 1991). "Replication-competent human mitochondrial DNA lacking the heavy-strand promoter region". Molecular and Cellular Biology. 11 (3): 1631–1637. doi:10.1128/MCB.11.3.1631. PMC   369459 . PMID   1996112.
• Attardi G, Chomyn A, Doolittle RF, Mariottini P, Ragan CI (1987). "Seven unidentified reading frames of human mitochondrial DNA encode subunits of the respiratory chain NADH dehydrogenase". Cold Spring Harbor Symposia on Quantitative Biology. 51 Pt 1 (1): 103–114. doi:10.1101/sqb.1986.051.01.013. PMID   3472707.
• Chomyn A, Cleeter MW, Ragan CI, Riley M, Doolittle RF, Attardi G (October 1986). "URF6, last unidentified reading frame of human mtDNA, codes for an NADH dehydrogenase subunit". Science. 234 (4776): 614–618. Bibcode:1986Sci...234..614C. doi:10.1126/science.3764430. PMID   3764430.
• Chomyn A, Mariottini P, Cleeter MW, Ragan CI, Matsuno-Yagi A, Hatefi Y, et al. (1985). "Six unidentified reading frames of human mitochondrial DNA encode components of the respiratory-chain NADH dehydrogenase". Nature. 314 (6012): 592–597. Bibcode:1985Natur.314..592C. doi:10.1038/314592a0. PMID   3921850. S2CID   32964006.
• Sanger F, Coulson AR, Barrell BG, Smith AJ, Roe BA (October 1980). "Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing". Journal of Molecular Biology. 143 (2): 161–178. doi:10.1016/0022-2836(80)90196-5. PMID   6260957.
• Montoya J, Ojala D, Attardi G (April 1981). "Distinctive features of the 5'-terminal sequences of the human mitochondrial mRNAs". Nature. 290 (5806): 465–470. Bibcode:1981Natur.290..465M. doi:10.1038/290465a0. PMID   7219535. S2CID   4358928.
• Horai S, Hayasaka K, Kondo R, Tsugane K, Takahata N (January 1995). "Recent African origin of modern humans revealed by complete sequences of hominoid mitochondrial DNAs". Proceedings of the National Academy of Sciences of the United States of America. 92 (2): 532–536. Bibcode:1995PNAS...92..532H. doi: 10.1073/pnas.92.2.532 . PMC   42775 . PMID   7530363.
• Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N (October 1999). "Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA". Nature Genetics. 23 (2): 147. doi: 10.1038/13779 . PMID   10508508. S2CID   32212178.
• Ingman M, Kaessmann H, Pääbo S, Gyllensten U (December 2000). "Mitochondrial genome variation and the origin of modern humans". Nature. 408 (6813): 708–713. Bibcode:2000Natur.408..708I. doi:10.1038/35047064. PMID   11130070. S2CID   52850476.
• Finnilä S, Lehtonen MS, Majamaa K (June 2001). "Phylogenetic network for European mtDNA". American Journal of Human Genetics. 68 (6): 1475–1484. doi:10.1086/320591. PMC   1226134 . PMID   11349229.
• Maca-Meyer N, González AM, Larruga JM, Flores C, Cabrera VM (2003). "Major genomic mitochondrial lineages delineate early human expansions". BMC Genetics. 2: 13. doi:10.1186/1471-2156-2-13. PMC   55343 . PMID   11553319.

This article incorporates text from the public domain Pfam and InterPro: IPR000883

This article incorporates text from the United States National Library of Medicine, which is in the public domain.