DHRS7B

Last updated
DHRS7B
DHRS7B homology model.png
Identifiers
Aliases DHRS7B , SDR32C1, CGI-93, dehydrogenase/reductase (SDR family) member 7B, dehydrogenase/reductase 7B
External IDs OMIM: 616160 MGI: 2384931 HomoloGene: 41044 GeneCards: DHRS7B
Orthologs
SpeciesHumanMouse
Entrez

25979

216820

Ensembl

ENSG00000109016

ENSMUSG00000042569

UniProt

Q6IAN0

Q99J47

RefSeq (mRNA)

NM_015510
NM_001330159

NM_001172112
NM_145428

RefSeq (protein)

NP_001317088
NP_056325

NP_001165583
NP_663403

Location (UCSC) Chr 17: 21.12 – 21.19 Mb Chr 11: 60.72 – 60.75 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Dehydrogenase/reductase (SDR family) member 7B is an enzyme encoded by the DHRS7B gene in humans, found on chromosome 17p11.2. [5] DHRS7B encodes a protein that is predicted to function in steroid hormone regulation. [6] [7] [8] A deletion in the chromosomal region 17p11.2 has been associated with Smith-Magenis Syndrome, a genetic developmental disorder. [9]

Contents

Gene

Overview

The DHRS7B gene is located on the positive strand of chromosome 17, beginning at position 21030258 and ending at position 21094836 (64579 bp). [10] DHRS7B contains seven exons with no predicted alternate splice forms, resulting in an 1841 bp mRNA product. [10] [11]

Upstream of DHRS7B on the negative strand of chromosome 17p11.2 are the genes Coiled-coil domain containing 144 family, N-terminal-like (CCDC144NL) and Ubiquitin specific peptidase 22 (USP22). [12] Downstream of DHSRS7B on the negative strand of chromosome 17p11.2 is the gene Transmembrane protein 11 (TMEM11), and on the positive strand is the gene Mitogen-activated protein kinase, kinase 3 (MAP2K3). [12]

Gene expression

Microarray and EST data indicates that the DHRS7B gene is highly expressed in the testes, thyroid, kidneys, and adipose tissues. There is moderate expression in the brain, pancreas, mammary glands, and ovaries. Finally, there is little expression in spleen, thymus, tonsils, bone marrow, and bladder. [13] [14]

Protein structure

The DHRS7B gene has a predicted protein product that is 325 amino acids, a molecular weight of 35.1 kDa, and an isoelectric point of 9.867. [15] [16] There is one predicted transmembrane domain in the protein sequence, a large neutrally charged region spanning residues 18-38. [15] [16] No signal peptides have been identified in DHRS7B; cellular localization remains unclear. [17]

DHRS7B is a member of the short chain dehydrogenase/reductase (SDR) superfamily and possesses characteristic features of an SDR within the protein sequence. The following table identifies sequences in the protein and corresponding function. [18]

Predicted Features of DHRS7B Protein BIOI Protein Representation.png
Predicted Features of DHRS7B Protein
SequenceFunction
"VVV"Valine-rich region, unknown function
"TGXXXGXG"NADP binding site
"NXXG"Possible active site motif
"DXXD"Adenine ring pocket motif
"GXXXXXSS"Possible active site motif
"SXYXXXK"Catalytic site, with upstream serine residue
"LXNNXG"Conserved region, unknown function
"NLS"N-glycosylation site

Interactions

In humans, DHRS7B has been shown to physically interact with other proteins such as Mediator complex subunit 19 (MED19) and Brain and reproductive expressed-modulator protein (BRE). [19] MED19 was found to interact with DHRS7B through a two hybrid screening approach and plays a role as a co-activator in regulated transcription of most RNA polymerase II dependent genes. [20] BRE is a component of the BRCA1-A complex, which recognizes Lys-63 linked ubiquitinated histones H2A and H2AX DNA lesion sites (identified using anti-tag coimmunoprecipitation). [21] Other proteins interacting with DHRS7B have only been identified through text mining.

Homology

Orthologs

Conservation of the DHRS7B protein sequence has been observed highly in mammals; moderately in reptiles, birds, fish and amphibians; minimally in invertebrates, insects, and fungi. [22]

Genus/SpeciesCommon NameAccession #Sequence LengthSequence IdentitySequence SimilarityNotes
Homo sapiensHumanNP_056325.2 325 aa100%100%DHRS7B
Pan troglodytesChimpanzeeXP_511344.2 325 aa99%99%Mammal
Pongo abelliSumatran orangutanNP_001127381 325 aa99%99%Mammal
Mustela putorius furoDomestic ferretAER97198 345 aa88%94%Mammal
Canis familiarisDogXP_536670 325 aa87%94%Mammal
Gallus gallusChickenXP_414804 309 aa73%87%Bird
Anolis carolinensisLizardXP_003226576 309 aa68%85%Reptile
Salmo salarSalmonACM08861 310 aa64%85%Fish
Xenopus (silurana) tropicalisWestern clawed frogNP_001072246 309 aa68%84%Amphibia
Drosophila melanogasterFruit flyNP_651717 326 aa45%63%Insect
Strongylocentrotus purpuratusPurple sea urchinXP_790920 344 aa34%50%Invertebrate
Saccharomyces cerevisiae S288CYeastNP_013953 267 aa33%49%Fungi

Paralogs

Paralogs of DHRS7B are all in the SDR superfamily and conservation of the SDR functional motifs was identified in a multiple sequence alignment. [22] [23]

Common NameAccession #Sequence LengthSequence IdentitySequence Similarity
DHRS7BNP_056325.2 325 aa100%100%
DHRS7CAA_147025.1 308 aa46%66%
DHRS7 CAH56402 375 aa37%53%
HBD1AAA58352 343 aa35%56%
RDH8EAW84062 331 aa34%53%
RDH16AAC39922 317 aa33%53%
KDSRNP002026 332 aa31%51%
HSD11B1AAK83653 292 aa29%50%
DHRS9AAH58883 319 aa29%50%
RDH5AAH28298 318 aa30%49%

Clinical significance

DHRS7B has been identified in the Smith-Magenis Syndrome region, where a deletion in this chromosomal region (17p11.2) causes a genetic developmental disorder. [8] In breast cancer cells expressing CD44 and CD24, DHRS7B expression was observed to be down regulated. [24] CD44 is an antigen found on the surface of most cell types and functions as a receptor that binds tissue macromolecules. Additionally, it acts as an adhesion molecule for leukocytes on peripheral lymphoid organs and inflammation sites. CD24 is associated with B-cells, epithelial cells, and dendritic cells, functioning as an adhesion molecule and shown to enhance a tumor cell's capability of metastasizing. [25]

Related Research Articles

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<span class="mw-page-title-main">NSDHL</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">RDH11</span>

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<span class="mw-page-title-main">DHRS2</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">DHRS1</span> Protein-coding gene in the species Homo sapiens

Dehydrogenase/reductase SDR family member 1, also known as Short chain dehydrogenase/reductase family 19C member 1 is an enzyme that in humans is encoded by the DHRS1 gene located on chromosome 14.

<span class="mw-page-title-main">RDH14</span>

Retinol dehydrogenase 14 is an enzyme that in humans is encoded by the RDH14 gene.

<span class="mw-page-title-main">DHRS3</span> Protein-coding gene in the species Homo sapiens

Short-chain dehydrogenase/reductase 3 is an enzyme that in humans is encoded by the DHRS3 gene.

<span class="mw-page-title-main">DHRS9</span> Protein-coding gene in the species Homo sapiens

Dehydrogenase/reductase SDR family member 9 is an enzyme that in humans is encoded by the DHRS9 gene.

<span class="mw-page-title-main">MLSTD2</span>

Fatty acyl-CoA reductase 1 is an enzyme that in humans is encoded by the FAR1 gene.

<span class="mw-page-title-main">TMEM63A</span>

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<span class="mw-page-title-main">DHRS4</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">DHRSX</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">DHRS7</span> Protein-coding gene in the species Homo sapiens

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<span class="mw-page-title-main">RDH13</span> Protein-coding gene in the species Homo sapiens

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References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000109016 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000042569 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ↑ "Entrez Gene: Dehydrogenase/reductase (SDR family) member 7B".
  6. ↑ "Genecards: DHRS7B Gene protein-coding GIFtS 47".
  7. ↑ Tannin GM, Agarwal AK, Monder C, New MI, White PC (September 1991). "The human gene for 11 beta-hydroxysteroid dehydrogenase. Structure, tissue distribution, and chromosomal localization". J. Biol. Chem. 266 (25): 16653–8. doi: 10.1016/S0021-9258(18)55351-5 . PMID   1885595.
  8. 1 2 "NCBI (National Center for Biotechnology Information)".
  9. ↑ "National Center for Biotechnology Information".
  10. 1 2 "NCBI: Nucleotide". National Center for Biotechnology Information. Retrieved 2012-04-29.
  11. ↑ "Softberry: FGENES". Softberry, Inc. Retrieved March 31, 2012.
  12. 1 2 "NCBI:MapViewer". NCBI. Retrieved 2012-03-30.
  13. ↑ "EST Profile Viewer".
  14. ↑ "GeneNote". Archived from the original on 2012-12-18.
  15. 1 2 Brendel V, Bucher P, Nourbakhsh IR, Blaisdell BE, Karlin S (March 1992). "Methods and algorithms for statistical analysis of protein sequences". Proc. Natl. Acad. Sci. U.S.A. 89 (6): 2002–6. Bibcode:1992PNAS...89.2002B. doi: 10.1073/pnas.89.6.2002 . PMC   48584 . PMID   1549558.
  16. 1 2 "Biology WorkBench 3.2". San Diego Supercomputer Center. Retrieved April 20, 2012.[ permanent dead link ]
  17. ↑ Petersen TN, Brunak S, von Heijne G, Nielsen H (2011). "SignalP 4.0: discriminating signal peptides from transmembrane regions". Nat. Methods. 8 (10): 785–6. doi: 10.1038/nmeth.1701 . PMID   21959131. S2CID   16509924.
  18. ↑ Oppermann U, Kavanagh K, Guo K, Ng SS, Lukacik P, Wu X, Dubinina E, Shafqat N, Bray J, Marsden BD, Sharma S, Vedadi M, Delft FV, Sundstrom M (2005). "SDR goes SGC: a Structural Genomics Initiative". In H Weiner, B Plapp, R Lindahl, E Maser (eds.). Enzymology and Molecular Biology of Carbonyl Metabolism. Vol. 12. West Lafayette, Ind: Purdue University Press. pp. 235–241. ISBN   1-55753-384-9.
  19. ↑ Kerrien S, Aranda B, Breuza L, Bridge A, Broackes-Carter F, Chen C, Duesbury M, Dumousseau M, Feuermann M, Hinz U, Jandrasits C, Jimenez RC, Khadake J, Mahadevan U, Masson P, Pedruzzi I, Pfeiffenberger E, Porras P, Raghunath A, Roechert B, Orchard S, Hermjakob H (January 2012). "The IntAct molecular interaction database in 2012". Nucleic Acids Res. 40 (Database issue): D841–6. doi:10.1093/nar/gkr1088. PMC   3245075 . PMID   22121220.
  20. ↑ Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl: 11858/00-001M-0000-0010-8592-0 . PMID   16169070. S2CID   8235923.
  21. ↑ Sowa ME, Bennett EJ, Gygi SP, Harper JW (July 2009). "Defining the human deubiquitinating enzyme interaction landscape". Cell. 138 (2): 389–403. doi:10.1016/j.cell.2009.04.042. PMC   2716422 . PMID   19615732.
  22. 1 2 "Basic Local Alignment Search Tool". National Center for Biotechnology Information. Retrieved March 3, 2012.
  23. ↑ Thompson JD, Higgins DG, Gibson TJ (November 1994). "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice". Nucleic Acids Res. 22 (22): 4673–80. doi:10.1093/nar/22.22.4673. PMC   308517 . PMID   7984417.
  24. ↑ Honeth G, Bendahl PO, Ringnér M, Saal LH, Gruvberger-Saal SK, Lövgren K, Grabau D, Fernö M, Borg A, Hegardt C (2008). "The CD44+/CD24- phenotype is enriched in basal-like breast tumors". Breast Cancer Res. 10 (3): R53. doi:10.1186/bcr2108. PMC   2481503 . PMID   18559090.
  25. ↑ Kuby J, Kindt TJ, Goldsby RA, Osborne BA (2007). Kuby immunology (6th ed.). San Francisco: W.H. Freeman. pp. Appendix A, 3–4. ISBN   978-1-4292-0211-4.
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