FF domain

Last updated
Domain found in FBP11, CA150, and PRP40
PDB 4FQG.jpg
Crystal structure of the TCERG1 FF4-6 tandem repeat domain
Identifiers
SymbolFF
Pfam PF01846
InterPro IPR002713
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary

FF domains are short protein domains of about 50 amino acids. The domain was discovered by multiple sequence alignments. FF domains have been found in various transcription factors, splicing proteins, and signaling proteins. FF domains and WW domains often occur together in proteins. [1]

The structure of the FF domain in CA150 (TCERG1) was solved in 2009 [2] with refinements in 2013. [3]

FF domains are protein interaction domains and thus can mediate the interaction among different proteins, such as various splicing proteins in the spliceosome. For instance, the interaction of yeast splicing proteins Prp40 and Luc7 is mediated by FF domains. [4]

References

  1. Bedford, M. T.; Leder, P. (July 1999). "The FF domain: a novel motif that often accompanies WW domains". Trends in Biochemical Sciences. 24 (7): 264–265. doi:10.1016/s0968-0004(99)01417-6. ISSN   0968-0004. PMID   10390614.
  2. Lu, Ming; Yang, Jun; Ren, Zhiyong; Sabui, Subir; Espejo, Alexsandra; Bedford, Mark T.; Jacobson, Raymond H.; Jeruzalmi, David; McMurray, John S.; Chen, Xiaomin (2009-10-23). "Crystal structure of the three tandem FF domains of the transcription elongation regulator CA150". Journal of Molecular Biology. 393 (2): 397–408. doi:10.1016/j.jmb.2009.07.086. ISSN   1089-8638. PMC   3319151 . PMID   19660470.
  3. Liu, Jiangxin; Fan, Shilong; Lee, Chul-Jin; Greenleaf, Arno L.; Zhou, Pei (April 2013). "Specific Interaction of the Transcription Elongation Regulator TCERG1 with RNA Polymerase II Requires Simultaneous Phosphorylation at Ser2, Ser5, and Ser7 within the Carboxyl-terminal Domain Repeat". Journal of Biological Chemistry. 288 (15): 10890–10901. doi: 10.1074/jbc.M113.460238 . PMC   3624469 . PMID   23436654.
  4. Ester, Claudia; Uetz, Peter (2008). "The FF domains of yeast U1 snRNP protein Prp40 mediate interactions with Luc7 and Snu71". BMC Biochemistry. 9 (1): 29. doi: 10.1186/1471-2091-9-29 . ISSN   1471-2091. PMC   2613882 . PMID   19014439.
This article incorporates text from the public domain Pfam and InterPro: IPR002713