TRNT1

TRNT1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1OU5, 4X4W
Identifiers
Aliases	TRNT1 , CCA1, MtCCA, CGI-47, SIFD, tRNA nucleotidyl transferase 1, RPEM
External IDs	OMIM: 612907; MGI: 1917297; HomoloGene: 9333; GeneCards: TRNT1; OMA:TRNT1 - orthologs
Gene location (Human) Chr. Chromosome 3 (human) [1] Band 3p26.2 Start 3,126,916 bp [1] End 3,150,879 bp [1]
Gene location (Mouse) Chr. Chromosome 6 (mouse) [2] Band 6|6 E1 Start 106,746,081 bp [2] End 106,759,435 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in endothelial cell secondary oocyte gonad Achilles tendon parietal pleura epithelium of colon pancreatic epithelial cell cartilage tissue metanephros testicle Top expressed in spermatocyte zygote spermatid genital tubercle secondary oocyte tail of embryo yolk sac ventricular zone embryo atrioventricular valve More reference expression data BioGPS n/a
Gene ontology Molecular function CTP:3'-cytidine-tRNA cytidylyltransferase activity transferase activity nucleotide binding tRNA binding nucleotidyltransferase activity ATP binding CTP:tRNA cytidylyltransferase activity RNA binding 5'-3' RNA polymerase activity ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Cellular component mitochondrial matrix intracellular anatomical structure mitochondrion nucleoplasm Biological process RNA processing tRNA 3'-terminal CCA addition tRNA processing tRNA 3'-end processing mitochondrial tRNA 3'-end processing Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 51095 70047 Ensembl ENSG00000072756 ENSMUSG00000013736 UniProt Q96Q11 Q8K1J6 RefSeq (mRNA) NM_001302946 NM_016000 NM_182916 NM_001242358 NM_001242360 NM_027296 RefSeq (protein) NP_001289875 NP_886552 NP_001354250 NP_001354251 NP_001354252 NP_001229287 NP_001229289 NP_081572 Location (UCSC) Chr 3: 3.13 – 3.15 Mb Chr 6: 106.75 – 106.76 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

tRNA-nucleotidyltransferase 1, is an enzyme that in humans is encoded by the TRNT1 gene. ^{[5] [6] [7]} This enzyme adds the nucleotide sequence CCA to the 3' end of tRNA, using ATP and CTP as substrates. The sequence creates the binding site for an amino acid. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000072756 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000013736 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W (Aug 2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC   310876 . PMID   10810093.
6. Nagaike T, Suzuki T, Tomari Y, Takemoto-Hori C, Negayama F, Watanabe K, Ueda T (Oct 2001). "Identification and characterization of mammalian mitochondrial tRNA nucleotidyltransferases". J Biol Chem. 276 (43): 40041–9. doi: 10.1074/jbc.M106202200 . PMID   11504732.
7. "Entrez Gene: TRNT1 tRNA nucleotidyl transferase, CCA-adding, 1".
8. Lizano E, Scheibe M, Rammelt C, Betat H, Mörl M (May 2008). "A comparative analysis of CCA-adding enzymes from human and E. coli: differences in CCA addition and tRNA 3'-end repair". Biochimie. 90 (5): 762–72. doi:10.1016/j.biochi.2007.12.007. PMID   18226598.

Further reading

• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC   310948 . PMID   11076863.
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC   311072 . PMID   11230166.
• Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC   1083732 . PMID   11256614.
• Reichert AS, Thurlow DL, Mörl M (2002). "A eubacterial origin for the human tRNA nucleotidyltransferase?". Biol. Chem. 382 (10): 1431–8. CiteSeerX   10.1.1.657.1235 . doi:10.1515/BC.2001.176. PMID   11727826. S2CID   230616.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Augustin MA, Reichert AS, Betat H, et al. (2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". J. Mol. Biol. 328 (5): 985–94. doi: 10.1016/S0022-2836(03)00381-4 . PMID   12729736.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC   528930 . PMID   15489336.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC   1356129 . PMID   16344560.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC   1347501 . PMID   16381901.
• Lizano E, Schuster J, Müller M, et al. (2007). "A splice variant of the human CCA-adding enzyme with modified activity". J. Mol. Biol. 366 (4): 1258–65. doi:10.1016/j.jmb.2006.12.016. PMID   17204286.