Fibrinoid necrosis

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Fibrinoid necrosis is a pathological lesion that affects blood vessels, and is characterized by the occurrence of endothelial damage, followed by leakage of plasma proteins, including fibrinogen, from the vessel lumen; these proteins infiltrate and deposit within the vessel walls, where fibrin polymerization subsequently ensues. [1] [2] [3] [4]

Contents

Although the term fibrinoid essentially means "fibrin-like", it has been confirmed through immunohistochemical analysis and electron microscopy that the areas referred to as "fibrin-like" do contain fibrin, whose predominant presence contributes to the bright, eosinophilic (pinkish) and structureless appearance of the affected vessels. [4] [5] [6] [7]

The earliest documented identification of fibrinoid changes dates back to 1880, when it was questioned whether these histological changes resulted from the deposition of a fibrinous exudate, or the degeneration and breakdown of collagen fibers. [8] [9]

The term fibrinoid was introduced to describe these changes, because distinguishing fibrinoid from hyaline deposits posed a significant challenge, as both exhibit a similar appearance under standard light microscopy. [4] [8] This morphological similarity necessitated the use of specialized histological staining techniques, such as phosphotungstic acid hematoxylin and various types of trichrome stains, to facilitate the distinction of fibrinoid material. Because these stains possess the ability to highlight and identify fibrin, this led to the term fibrinoid, which means "fibrin-like", being used to describe the affected vessels. [4]

Nevertheless, as early as 1957, fibrin was indeed identified within fibrinoid, and by 1982, this understanding had advanced, with many researchers recognizing fibrinoid as a complex structure primarily composed of fibrin interwoven with various plasma proteins. [8]

A renal biopsy from a patient with anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis reveals a lesion characterized by bright eosinophilia on H&E staining (yellow arrow, left) and intense red staining with trichrome (right), confirming the presence of fibrinoid necrosis. Fibrinoid necrosis.jpg
A renal biopsy from a patient with anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis reveals a lesion characterized by bright eosinophilia on H&E staining (yellow arrow, left) and intense red staining with trichrome (right), confirming the presence of fibrinoid necrosis.
An H&E micrograph showing (intensely pink) fibrinoid necrosis (large blood vessel - right of image) in a case of vasculitis (eosinophilic granulomatosis with polyangiitis). Churg-Strauss syndrome - high mag.jpg
An H&E micrograph showing (intensely pink) fibrinoid necrosis (large blood vessel – right of image) in a case of vasculitis (eosinophilic granulomatosis with polyangiitis).

Nomenclature

A misnomer

The term fibrinoid necrosis is, in fact, considered a misnomer, [1] [10] as the intense eosinophilic staining of the accumulated plasma proteins masks the true nature of the underlying changes in the blood vessel, and makes it virtually impossible to definitively determine whether the cells of the vessel wall are actually undergoing necrosis. [1] [11]

A 2000 review stated that "whether the lesion is truly necrotic, in the sense that it reflects the result of unprogrammed cell death, has never been investigated in depth", [8] and an electron microscopy study examining fibrinoid necrosis in rat models with induced pulmonary hypertension found that fibrinoid changes weren't necessarily associated with necrosis of the smooth muscles of the media, and therefore recommended using the term fibrinoid vasculosis instead. [6]

However, despite the inaccuracy, the microscopic characteristics of fibrinoid changes strongly resemble those of necrotic tissue, which is why the term fibrinoid necrosis continues to be used, even though it may not fully reflect the true underlying process. [10]

Fibrinoid necrosis and lipohyalinosis

In 1971, CM Fisher, a pioneering figure in cerebral vascular diseases, proposed using the term lipohyalinosis as a replacement for fibrinoid necrosis, based on his observation that the affected fibrinoid areas also contained lipid. [4]

The term lipohyalinosis was intended to serve as a synonym for fibrinoid necrosis, yet it is strictly used to describe the pathological fibrinoid changes in the cerebral vessels of patients with malignant hypertension. Even though the same pathological process, that affects cerebral blood vessels in malignant hypertension, also occurs in the arterioles of other organs, such as the kidneys and mesentery, lipohyalinosis is not used to describe these changes outside the brain, and fibrinoid necrosis remains the more widely recognized term for similar processes in other organs. [4]

However, a common misconception in many textbooks is the failure to clarify that lipohyalinosis and fibrinoid necrosis are actually two descriptions of the same pathological process. [12]

Instead of recognizing their equivalence, they are often presented as distinct stages, where lipohyalinosis is mistakenly described as a later consequence of fibrinoid necrosis, or lipohyalinosis is sometimes erroneously used interchangeably with arteriolosclerosis, which is a much broader term used to describe pathological changes in small arteries caused by a variety of conditions. Mislabeling lipohyalinosis as arteriolosclerosis overlooks the specific nature of lipohyalinosis as a condition involving fibrinoid necrosis (a particular form of vascular injury) and contributes to confusion. [12]

Localization

Fibrinoid necrosis predominantly affects small blood vessels, such as arterioles and glomeruli, [3] but it can also involve medium-sized vessels, as observed in conditions like polyarteritis nodosa. [13] It can also exhibit a highly segmental distribution, where the fibrinoid material does not uniformly coat the affected vessel but instead appears in isolated patches that are spaced along the length of the vessel wall. [4] [14]

Fibrinoid infiltration in affected vessels may be confined to the subintimal region, as the ground substance of the intima and the inner elastic lamina often act as a barrier, limiting further penetration of fibrin into the arterial wall. [6] However, if the internal elastic lamina is disrupted, fibrin may extend into the media, where it is typically contained by the outer elastic lamina, potentially spreading circumferentially along its inner surface. [6] [15] In some cases, fibrin may extend into the adventitia or even escape from the vessels into surrounding perivascular tissue or adjacent spaces. This phenomenon is observed in conditions such as antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis, where fibrin can infiltrate the urinary space near glomerular capillaries or the air space adjacent to alveolar capillaries. [16]

Associated diseases

Fibrinoid necrosis is observed in a wide range of pathological conditions such as:

  1. Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis: Fibrinoid necrosis is a classical finding in these small-vessel vasculitides, and it has been referred to as "the ANCA-associated lesion" even though it occurs in other conditions as well. [8]
  2. Polyarteritis nodosa (PAN): The hallmark morphological characteristic of PAN is fibrinoid necrosis. [1] In contrast, fibrinoid necrosis in Kawasaki disease is less pronounced than in PAN, despite both being forms of medium-sized vessel vasculitides. [17]
  3. Systemic lupus erythematosus. [14]
  4. Leucocytoclastic vasculitis. [18]
  5. Systemic sclerosis, arthus reaction (a type III hypersensitivity reaction) and rheumatoid subcutaneous nodules. [17]
An H&E-stained micrograph of a rheumatoid nodule reveals its characteristic histological structure, featuring a central core composed of fibrinoid necrosis, and surrounding this core is a layer of palisading macrophages and epithelioid histiocytes. Histopathology of a rheumatoid nodule.png
An H&E-stained micrograph of a rheumatoid nodule reveals its characteristic histological structure, featuring a central core composed of fibrinoid necrosis, and surrounding this core is a layer of palisading macrophages and epithelioid histiocytes.

Pathogenesis

Fibrinoid necrosis occurs as a consequence of endothelial injury, which permits the leakage of plasma proteins into the blood vessel walls. [2] [3] [28] This endothelial damage may arise due to a variety of underlying factors; for instance:

Endothelial cell damage results in the loss of the normal barrier function, and allows plasma components, including coagulation factors, to escape from the bloodstream and leak out into the blood vessel walls and the surrounding spaces. The coagulation factors that leak from the damaged blood vessels interact with various thrombogenic substances, such as tissue factor, which culminates in the formation of fibrin, whose accumulation leads to the characteristic appearance of fibrinoid necrosis. [5] [33]

Clinical relevance

Pathological consequences

Ischemia

Hemorrhage

Whenever hypertension induces fibrinoid necrosis in the small cerebral arteries, this considerably raises the risk of intracerebral hemorrhage (ICH) due to two main factors: [31]

  1. The deposition of fibrinoid material in the vessel wall leads to thickening of the arterial walls, making them progressively more rigid and less elastic.
  2. The narrowing of the arterial lumens further exacerbates this by raising intraluminal pressure.

As a result, the small cerebral arteries become more fragile and prone to rupture, which may ultimately lead to ICH.

Retinal detachment

When blood pressure rises significantly, as in malignant hypertension or eclampsia, retinal arterioles can undergo fibrinoid necrosis, reducing blood supply to the choriocapillaris, which is responsible for nourishing the retinal pigment epithelium (RPE). Ischemia disrupts RPE function, compromises the blood-retinal barrier and causes fluid leakage into the subretinal space, and the development of exudative retinal detachment. [35] [36]

Diagnostic value

Fibrinoid necrosis serves as an important diagnostic clue in recognizing vascular pathologies, and helping to guide further investigation and treatment; for instance:

  1. Neutrophilic infiltration in and around the vessel wall with leukocytoclasia.
  2. Fibrinoid necrosis.
  3. Vessel wall and tissue damage.

The lack of fibrinoid necrosis and inflammatory infiltration in the vessel may preclude the diagnosis of classic LCV, and necessitates further evaluation. [40] However, these histological features tend to progress gradually over time, and a biopsy taken too early or too late might miss the "textbook" full-blown changes of LCV. [39]

Prognostic value

Fibrinoid necrosis is included in the modified National Institutes of Health (NIH) activity index for lupus nephritis (LN), which is a scoring system used to assess the severity of LN based on histopathologic findings from kidney biopsies. [14]

The activity index is based on the evaluation of six histologic features that indicate active inflammation, each of which is assigned a score from 0-3 depending on the degree of glomerular involvement. [14] [41] The score of fibrinoid necrosis and cellular/fibrocellular crescents is multiplied by two, because these two lesions were considered to be associated with a higher level of severity; [42] this gives the activity index a total score of 0-24. [note 2]

The activity index correlates with the level of active inflammation in LN, [41] and serves as a general framework for guiding treatment decisions; the higher the NIH activity score, the more intensive the immunosuppressive treatment required. [43]

Notes

  1. This figure illustrates that Masson's trichrome stain also helps to distinguish between fibrinoid necrosis, and sclerosis in renal biopsies.
  2. This figure illustrates the modified NIH activity and chronicity indices for lupus nephritis.

References

  1. 1 2 3 4 5 Strayer D, Rubin E, Saffitz J, Schiller A (2015). Rubin's Pathology: Clinicopathologic Foundations of Medicine (7th ed.). Philadelphia, Penn.: Wolters Kluwer. ISBN   978-1-4511-8390-0.
  2. 1 2 Miller M, Zachary J (2017). "Mechanisms and Morphology of Cellular Injury, Adaptation, and Death". Pathologic Basis of Veterinary Disease: 2–34. doi:10.1016/B978-0-323-35775-3.00001-1. ISBN   978-0-323-35775-3. PMC   7171462 .
  3. 1 2 3 Chen H, Liu Y, Wei L, Wang H, Zheng Z, Yan T, et al. (December 2023). "The effect of fibrinoid necrosis on the clinical features and outcomes of primary IgA nephropathy". BMC Nephrology. 24 (1) 366. doi: 10.1186/s12882-023-03419-4 . PMC   10712095 . PMID   38082385.
  4. 1 2 3 4 5 6 7 8 Rosenblum WI (October 2008). "Fibrinoid necrosis of small brain arteries and arterioles and miliary aneurysms as causes of hypertensive hemorrhage: a critical reappraisal". Acta Neuropathologica. 116 (4): 361–369. doi:10.1007/s00401-008-0416-9. PMID   18642006.
  5. 1 2 Hano H, Takagi I, Nagatsuma K, Lu T, Meng C, Chiba S (January 2007). "An autopsy case showing massive fibrinoid necrosis of the portal tracts of the liver with cholangiographic findings similar to those of primary sclerosing cholangitis". World Journal of Gastroenterology. 13 (4): 639–642. doi: 10.3748/wjg.v13.i4.639 (inactive 12 July 2025). PMC   4065992 . PMID   17278236.{{cite journal}}: CS1 maint: DOI inactive as of July 2025 (link)
  6. 1 2 3 4 5 Heath D, Smith P (October 1978). "The electron microscopy of "fibrinoid necrosis" in pulmonary arteries". Thorax. 33 (5): 579–595. doi:10.1136/thx.33.5.579. PMC   470940 . PMID   31704.
  7. Wiener J, Spiro D, Lattes RG (September 1965). "The Cellular Pathology of Experimental Hypertension. II. Arteriolar Hyalinosis and Fibrinoid Change". The American Journal of Pathology. 47 (3): 457–485. PMC   1920447 . PMID   14334752.
  8. 1 2 3 4 5 Bajema IM, Bruijn JA (July 2000). "What stuff is this! A historical perspective on fibrinoid necrosis". The Journal of Pathology. 191 (3): 235–238. doi: 10.1002/(SICI)1096-9896(0000)9999:9999<N/A::AID-PATH610>3.0.CO;2-I . PMID   10878543.
  9. Scott GB (June 1968). "Trypan blue and the generalized Shwartzman reaction. The nature and formation of fibrinoid material in the pulmonary arteries". British Journal of Experimental Pathology. 49 (3): 251–256. PMC   2093816 . PMID   5665438.
  10. 1 2 Cross SS (2013). Underwood's Pathology: A Clinical Approach (6th ed.). Churchill Livingstone. ISBN   978-0-7020-4672-8.
  11. Jennette JC, Falk RJ, Hu P, Xiao H (January 2013). "Pathogenesis of antineutrophil cytoplasmic autoantibody-associated small-vessel vasculitis". Annual Review of Pathology. 8: 139–160. doi:10.1146/annurev-pathol-011811-132453. PMC   5507606 . PMID   23347350.
  12. 1 2 Rosenblum WI (September 2021). "Lipohyalinosis and Fibrinoid: Consistent and Important Failure to Understand These Are Synonyms". Journal of Neuropathology and Experimental Neurology. 80 (8): 802–803. doi: 10.1093/jnen/nlab009 . PMID   33684938.
  13. Aw DK, Vijaykumar K, Cheng SC, Tang TY, Tay JS, Choke ET (December 2023). "A case of severe polyarteritis nodosa with critical limb threatening ischemia-promising treatment with sirolimus drug-coated angioplasty". Journal of Vascular Surgery Cases and Innovative Techniques. 9 (4) 101266. doi:10.1016/j.jvscit.2023.101266. PMC   10725072 . PMID   38106351.
  14. 1 2 3 4 Bajema IM, Wilhelmus S, Alpers CE, Bruijn JA, Colvin RB, Cook HT, et al. (April 2018). "Revision of the International Society of Nephrology/Renal Pathology Society classification for lupus nephritis: clarification of definitions, and modified National Institutes of Health activity and chronicity indices". Kidney International. 93 (4): 789–796. doi: 10.1016/j.kint.2017.11.023 . hdl: 10044/1/57351 . PMID   29459092.
  15. Cassisa A, Cima L (April 2024). "Cutaneous vasculitis: insights into pathogenesis and histopathological features". Pathologica. 116 (2) 6: 119–133. doi:10.32074/1591-951X-985. PMC   11138767 . PMID   38767544.
  16. Sinico R, Guillevin L (2020). Anti-Neutrophil Cytoplasmic Antibody (ANCA) Associated Vasculitis. Rare Diseases of the Immune System. Springer Nature. doi:10.1007/978-3-030-02239-6. ISBN   978-3-030-02238-9.
  17. 1 2 3 4 Kumar V, Abbas AK, Aster JC (24 June 2014). Robbins & Cotran Pathologic Basis of Disease (9th ed.). Elsevier Health Sciences. ISBN   978-0-323-29635-9.
  18. 1 2 Baigrie D, Crane JS (January 2024), "Leukocytoclastic Vasculitis.", StatPearls, Treasure Island, Florida (FL): StatPearls Publishing, PMID   29489227
  19. Naranjo M, Chauhan S, Paul M (January 2024), "Malignant Hypertension.", StatPearls, Treasure Island, Florida (FL): StatPearls Publishing, PMID   29939523
  20. 1 2 Kasper DL, Fauci AS, Hauser SL, Longo DL, Jameson JL, Loscalzo J (6 February 2018). Harrison's Principles of Internal Medicine 20/E (Vol.1 & Vol.2) (ebook) (20th ed.). McGraw Hill Professional. ISBN   978-1-259-64404-7.
  21. Gusella A, Martignoni G, Giacometti C (July 2024). "Behind the Curtain of Abnormal Placentation in Pre-Eclampsia: From Molecular Mechanisms to Histological Hallmarks". International Journal of Molecular Sciences. 25 (14): 7886. doi: 10.3390/ijms25147886 . PMC   11277090 . PMID   39063129.
  22. Basri NI, Murthi P, Abd Rahman R (September 2024). "Hydroxychloroquine as an Adjunct Therapy for Diabetes in Pregnancy". International Journal of Molecular Sciences. 25 (17): 9681. doi: 10.3390/ijms25179681 . PMC   11395545 . PMID   39273629.
  23. Agarwal A, Ajmera P, Sharma P, Kanekar S (April 2024). "Cerebral microbleeds: Causes, clinical relevance, and imaging approach - A narrative review". Journal of Neurosciences in Rural Practice. 15 (2) 169: 169–181. doi:10.25259/JNRP_351_2023. PMC   11090589 . PMID   38746527.
  24. Gopallawa I, Dehinwal R, Bhatia V, Gujar V, Chirmule N (2023). "A four-part guide to lung immunology: Invasion, inflammation, immunity, and intervention". Frontiers in Immunology. 14 1119564. doi: 10.3389/fimmu.2023.1119564 . PMC   10102582 . PMID   37063828.
  25. Septimiu-Radu S, Gadela T, Gabriela D, Oancea C, Rosca O, Lazureanu VE, et al. (March 2023). "A Systematic Review of Lung Autopsy Findings in Elderly Patients after SARS-CoV-2 Infection". Journal of Clinical Medicine. 12 (5): 2070. doi: 10.3390/jcm12052070 . PMC   10004532 . PMID   36902856.
  26. Weeratunga P, Moller DR, Ho LP (January 2024). "Immune mechanisms of granuloma formation in sarcoidosis and tuberculosis". The Journal of Clinical Investigation. 134 (1) e175264. doi:10.1172/JCI175264. PMC   10760966 . PMID   38165044.
  27. Scheepens JC, Taphoorn MJ, Koekkoek JA (November 2024). "Patient-reported outcomes in neuro-oncology". Current Opinion in Oncology. 36 (6): 560–568. doi:10.1097/CCO.0000000000001078. PMC   11460742 . PMID   38984633.
  28. Adigun R, Basit H, Murray J (January 2024), "Cell Liquefactive Necrosis.", StatPearls, Treasure Island, Florida (FL): StatPearls Publishing, PMID   28613685
  29. Gluhovschi C, Gadalean F, Velciov S, Nistor M, Petrica L (November 2023). "Three Diseases Mediated by Different Immunopathologic Mechanisms-ANCA-Associated Vasculitis, Anti-Glomerular Basement Membrane Disease, and Immune Complex-Mediated Glomerulonephritis-A Common Clinical and Histopathologic Picture: Rapidly Progressive Crescentic Glomerulonephritis". Biomedicines. 11 (11): 2978. doi: 10.3390/biomedicines11112978 . PMC   10669599 . PMID   38001978.
  30. 1 2 Mohan H (31 October 2014). Textbook of Pathology (7th ed.). Jaypee Brothers Medical Publishers Pvt. Limited. ISBN   978-93-5152-369-7.
  31. 1 2 Huang B, Chen A, Sun Y, He Q (June 2024). "The Role of Aging in Intracerebral Hemorrhage". Brain Sciences. 14 (6): 613. doi: 10.3390/brainsci14060613 . PMC   11201415 . PMID   38928613.
  32. Romano A, Moltoni G, Blandino A, Palizzi S, Romano A, de Rosa G, et al. (October 2023). "Radiosurgery for Brain Metastases: Challenges in Imaging Interpretation after Treatment". Cancers. 15 (20): 5092. doi: 10.3390/cancers15205092 . PMC   10605307 . PMID   37894459.
  33. Jennette JC, Falk RJ (August 2014). "Pathogenesis of antineutrophil cytoplasmic autoantibody-mediated disease". Nature Reviews. Rheumatology. 10 (8): 463–473. doi: 10.1038/nrrheum.2014.103 . PMID   25003769.
  34. Marcinkowska W, Zielinska N, Szewczyk B, Łabętowicz P, Głowacka M, Olewnik Ł (October 2024). "Morphological Variability of the Sural Nerve and Its Clinical Significance". Journal of Clinical Medicine. 13 (20): 6055. doi: 10.3390/jcm13206055 . PMC   11508416 . PMID   39458004.
  35. Mishra P, Kanaujia V, Kesarwani D, Sharma K, Nanda J, Mishra P (June 2024). "Visual Outcomes in Malignant Hypertensive Retinopathy Cases: A Clinical and Spectral Domain Optical Coherence Tomography Study". Cureus. 16 (6): e62945. doi: 10.7759/cureus.62945 . PMC   11265969 . PMID   39050341.
  36. Hayreh SS, Hayreh SB (September 2023). "Uveal vascular bed in health and disease: lesions produced by occlusion of the uveal vascular bed and acute uveal ischaemic lesions seen clinically. Paper 2 of 2". Eye. 37 (13): 2617–2648. doi:10.1038/s41433-023-02417-y. PMC   10482881 . PMID   37185956.
  37. Monti S, Schäfer VS, Muratore F, Salvarani C, Montecucco C, Luqmani R (2023). "Updates on the diagnosis and monitoring of giant cell arteritis". Frontiers in Medicine. 10 1125141. doi: 10.3389/fmed.2023.1125141 . hdl: 11380/1299527 . PMC   9995793 . PMID   36910481.
  38. Matsuo T, Hiramatsu-Asano S, Sawachika H, Nishimura H (June 2023). "ANCA-associated vasculitis with scleritis, corneal melt, and perforation rescued by rituximab: Case report and literature review". Clinical Case Reports. 11 (6) e7595. doi:10.1002/ccr3.7595. PMC   10282114 . PMID   37351359.
  39. 1 2 Fraticelli P, Benfaremo D, Gabrielli A (June 2021). "Diagnosis and management of leukocytoclastic vasculitis". Internal and Emergency Medicine. 16 (4): 831–841. doi:10.1007/s11739-021-02688-x. PMC   8195763 . PMID   33713282.
  40. Oprinca GC, Mohor CI, Oprinca-Muja A, Hașegan A, Cristian AN, Fleacă SR, et al. (February 2024). "Unveiling the Pathological Mechanisms of Death Induced by SARS-CoV-2 Viral Pneumonia". Microorganisms. 12 (3): 459. doi: 10.3390/microorganisms12030459 . PMC   10972137 . PMID   38543510.
  41. 1 2 Mina R, Abulaban K, Klein-Gitelman MS, Eberhard BA, Ardoin SP, Singer N, et al. (February 2016). "Validation of the Lupus Nephritis Clinical Indices in Childhood-Onset Systemic Lupus Erythematosus". Arthritis Care & Research. 68 (2): 195–202. doi:10.1002/acr.22651. PMC   4720587 . PMID   26213987.
  42. Choi SE, Fogo AB, Lim BJ (March 2023). "Histologic evaluation of activity and chronicity of lupus nephritis and its clinical significance". Kidney Research and Clinical Practice. 42 (2): 166–173. doi:10.23876/j.krcp.22.083. PMC   10085727 . PMID   37037479.
  43. Fava A, Buyon J, Magder L, Hodgin J, Rosenberg A, Demeke DS, et al. (January 2024). "Urine proteomic signatures of histological class, activity, chronicity, and treatment response in lupus nephritis". JCI Insight. 9 (2) e172569. doi:10.1172/jci.insight.172569. PMC   10906224 . PMID   38258904.