POLE (gene)

POLE
Identifiers
Aliases	POLE , CRCS12, FILS, POLE1, polymerase (DNA) epsilon, catalytic subunit, DNA polymerase epsilon, catalytic subunit, IMAGEI
External IDs	OMIM: 174762 MGI: 1196391 HomoloGene: 4539 GeneCards: POLE
Gene location (Human) Chr. Chromosome 12 (human) [1] Band 12q24.33 Start 132,623,753 bp [1] End 132,687,376 bp [1]
Gene location (Mouse) Chr. Chromosome 5 (mouse) [2] Band 5 F|5 53.45 cM Start 110,434,172 bp [2] End 110,485,340 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sural nerve spleen skin of abdomen ganglionic eminence right lobe of liver gastric mucosa body of stomach bone marrow cells popliteal artery gastrocnemius muscle Top expressed in otic placode saccule internal carotid artery maxillary prominence utricle primitive streak external carotid artery spermatid vas deferens somite More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding DNA binding 4 iron, 4 sulfur cluster binding nucleotidyltransferase activity iron-sulfur cluster binding zinc ion binding chromatin binding metal ion binding nucleic acid binding DNA-directed DNA polymerase activity single-stranded DNA 3'-5' exodeoxyribonuclease activity protein binding Cellular component plasma membrane nucleoplasm nucleus epsilon DNA polymerase complex Biological process nucleotide-excision repair, DNA gap filling DNA synthesis involved in DNA repair DNA biosynthetic process embryonic organ development nucleic acid phosphodiester bond hydrolysis cellular response to DNA damage stimulus base-excision repair, gap-filling DNA repair DNA replication leading strand elongation DNA replication proofreading G1/S transition of mitotic cell cycle mitotic cell cycle DNA replication initiation telomere maintenance via semi-conservative replication Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5426 18973 Ensembl ENSG00000177084 ENSMUSG00000007080 UniProt Q07864 Q9WVF7 RefSeq (mRNA) NM_006231 NM_011132 RefSeq (protein) NP_006222 NP_035262 Location (UCSC) Chr 12: 132.62 – 132.69 Mb Chr 5: 110.43 – 110.49 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

DNA polymerase epsilon catalytic subunit is an enzyme that in humans is encoded by the POLE gene. ^{[5] [6]} It is the central catalytic subunit of DNA polymerase epsilon.

Clinical significance

POLE, along with POLD1, has in 2013 been associated with multiple adenoma. ^[7]

Interactions

POLE has been shown to interact with RAD17. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000177084 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000007080 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Szpirer J, Pedeutour F, Kesti T, Riviere M, Syväoja JE, Turc-Carel C, Szpirer C (Aug 1994). "Localization of the gene for DNA polymerase epsilon (POLE) to human chromosome 12q24.3 and rat chromosome 12 by somatic cell hybrid panels and fluorescence in situ hybridization". Genomics. 20 (2): 223–6. doi:10.1006/geno.1994.1156. PMID   8020968.
6. "Entrez Gene: POLE polymerase (DNA directed), epsilon".
7. Palles C, Cazier JB, Howarth KM, Domingo E, Jones AM, Broderick P, Kemp Z, Spain SL, Guarino E, Guarino Almeida E, Salguero I, Sherborne A, Chubb D, Carvajal-Carmona LG, Ma Y, Kaur K, Dobbins S, Barclay E, Gorman M, Martin L, Kovac MB, Humphray S, Lucassen A, Holmes CC, Bentley D, Donnelly P, Taylor J, Petridis C, Roylance R, Sawyer EJ, Kerr DJ, Clark S, Grimes J, Kearsey SE, Thomas HJ, McVean G, Houlston RS, Tomlinson I (2013). "Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas". Nat. Genet. 45 (2): 136–44. doi:10.1038/ng.2503. PMC   3785128 . PMID   23263490.
8. Post SM, Tomkinson AE, Lee EY (Oct 2003). "The human checkpoint Rad protein Rad17 is chromatin-associated throughout the cell cycle, localizes to DNA replication sites, and interacts with DNA polymerase epsilon". Nucleic Acids Res. England. 31 (19): 5568–75. doi:10.1093/nar/gkg765. PMC   206465 . PMID   14500819.

Further reading

• Popanda O, Thielmann HW (1992). "The function of DNA polymerases in DNA repair synthesis of ultraviolet-irradiated human fibroblasts". Biochim. Biophys. Acta. 1129 (2): 155–60. doi:10.1016/0167-4781(92)90480-N. PMID   1730053.
• Kesti T, Frantti H, Syväoja JE (1993). "Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase epsilon". J. Biol. Chem. 268 (14): 10238–45. doi: 10.1016/S0021-9258(18)82195-0 . PMID   8486689.
• Mozzherin DJ, Fisher PA (1996). "Human DNA polymerase epsilon: enzymologic mechanism and gap-filling synthesis". Biochemistry. 35 (11): 3572–7. doi:10.1021/bi952142p. PMID   8639508.
• Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme". Mol. Cell. Biol. 17 (4): 1817–23. doi:10.1128/mcb.17.4.1817. PMC   232028 . PMID   9121429.
• Coll JM, Hickey RJ, Cronkey EA, Jiang HY, Schnaper L, Lee MY, Uitto L, Syvaoja JE, Malkas LH (1998). "Mapping specific protein-protein interactions within the core component of the breast cell DNA synthesome". Oncol. Res. 9 (11–12): 629–39. PMID   9563011.
• Huang D, Pospiech H, Kesti T, Syväoja JE (1999). "Structural organization and splice variants of the POLE1 gene encoding the catalytic subunit of human DNA polymerase epsilon". Biochem. J. 339 (3): 657–65. doi:10.1042/0264-6021:3390657. PMC   1220202 . PMID   10215605.
• Araújo SJ, Tirode F, Coin F, Pospiech H, Syväoja JE, Stucki M, Hübscher U, Egly JM, Wood RD (2000). "Nucleotide excision repair of DNA with recombinant human proteins: definition of the minimal set of factors, active forms of TFIIH, and modulation by CAK". Genes Dev. 14 (3): 349–59. doi:10.1101/gad.14.3.349. PMC   316364 . PMID   10673506.
• Li Y, Pursell ZF, Linn S (2000). "Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon". J. Biol. Chem. 275 (30): 23247–52. doi: 10.1074/jbc.M002548200 . PMID   10801849.
• Mäkiniemi M, Hillukkala T, Tuusa J, Reini K, Vaara M, Huang D, Pospiech H, Majuri I, Westerling T, Mäkelä TP, Syväoja JE (2001). "BRCT domain-containing protein TopBP1 functions in DNA replication and damage response". J. Biol. Chem. 276 (32): 30399–406. doi: 10.1074/jbc.M102245200 . PMID   11395493.
• Fuss J, Linn S (2002). "Human DNA polymerase epsilon colocalizes with proliferating cell nuclear antigen and DNA replication late, but not early, in S phase". J. Biol. Chem. 277 (10): 8658–66. doi: 10.1074/jbc.M110615200 . PMID   11741962.
• Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi: 10.1074/jbc.M206194200 . PMID   12171929.
• Post SM, Tomkinson AE, Lee EY (2003). "The human checkpoint Rad protein Rad17 is chromatin-associated throughout the cell cycle, localizes to DNA replication sites, and interacts with DNA polymerase epsilon". Nucleic Acids Res. 31 (19): 5568–75. doi:10.1093/nar/gkg765. PMC   206465 . PMID   14500819.
• Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. PMID   14667819.
• Clark AG, Glanowski S, Nielsen R, Thomas PD, Kejariwal A, Todd MA, Tanenbaum DM, Civello D, Lu F, Murphy B, Ferriera S, Wang G, Zheng X, White TJ, Sninsky JJ, Adams MD, Cargill M (2003). "Inferring nonneutral evolution from human-chimp-mouse orthologous gene trios". Science. 302 (5652): 1960–3. Bibcode:2003Sci...302.1960C. doi:10.1126/science.1088821. PMID   14671302. S2CID   6682593.
• Rytkönen AK, Vaara M, Nethanel T, Kaufmann G, Sormunen R, Läärä E, Nasheuer HP, Rahmeh A, Lee MY, Syväoja JE, Pospiech H (2006). "Distinctive activities of DNA polymerases during human DNA replication". FEBS J. 273 (13): 2984–3001. doi:10.1111/j.1742-4658.2006.05310.x. PMID   16762037. S2CID   32819159.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.