| 2021 TK Sparta Prague Open | |
|---|---|
| Date | 9–15 August |
| Category | ATP Challenger Tour |
| Draw | 32S / 16D |
| Surface | Clay |
| Location | Prague, Czech Republic |
| Venue | TK Sparta Praha |
| Champions | |
| Singles | |
 Dalibor Svrčina | |
| Doubles | |
| Jonáš Forejtek / Michael Vrbenský | |
The 2021 TK Sparta Prague Open was a professional tennis tournament played on clay courts. It was part of the 2021 ATP Challenger Tour. It took place in Prague, Czech Republic between 9 and 15 August 2021.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 SRB | Nikola Milojević | 161 | 1 |
 KAZ | Dmitry Popko | 183 | 2 |
 ITA | Lorenzo Giustino | 234 | 3 |
 ARG | Facundo Mena | 254 | 4 |
 CHI | Nicolás Jarry | 263 | 5 |
| ITA | Riccardo Bonadio | 271 | 6 |
 ESP | Adrián Menéndez Maceiras | 287 | 7 |
| CZE | Michael Vrbenský | 288 | 8 |
The following players received wildcards into the singles main draw:
Toby Kodat Martin Krumich Dalibor Svrčina The following player received entry into the singles main draw using a protected ranking:
The following players received entry from the qualifying draw:
Dalibor Svrčina def. Dmitry Popko 6–0, 7–5. Jonáš Forejtek / Michael Vrbenský def. 
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