| 2021 Tallahassee Tennis Challenger | |
|---|---|
| Date | 19–25 April |
| Edition | 21st |
| Surface | Green clay |
| Location | Tallahassee, Florida, United States |
| Champions | |
| Singles | |
 Jenson Brooksby | |
| Doubles | |
 Orlando Luz / Rafael Matos | |
The 2021 Tallahassee Tennis Challenger was a professional tennis tournament played on green clay courts. It was the 21st edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Tallahassee, Florida, United States between April 19 and April 25, 2021.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| BRA | Thiago Seyboth Wild | 123 | 1 |
| USA | Denis Kudla | 124 | 2 |
 IND | Prajnesh Gunneswaran | 138 | 3 |
 TPE | Jason Jung | 151 | 4 |
 CRO | Ivo Karlović | 170 | 5 |
 ARG | Juan Manuel Cerúndolo | 174 | 6 |
 KAZ | Dmitry Popko | 180 | 7 |
| USA | Michael Mmoh | 181 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
Jenson Brooksby def. Bjorn Fratangelo 6–3, 4–6, 6–3. Orlando Luz / Rafael Matos def. Sekou Bangoura / Donald Young 7–6(7–2), 6–2.