PTPN12

PTPN12
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5J8R
Identifiers
Aliases	PTPN12 , PTP-PEST, PTPG1, protein tyrosine phosphatase, non-receptor type 12, protein tyrosine phosphatase non-receptor type 12
External IDs	OMIM: 600079; MGI: 104673; HomoloGene: 37691; GeneCards: PTPN12; OMA:PTPN12 - orthologs
Gene location (Human) Chr. Chromosome 7 (human) [1] Band 7q11.23 Start 77,537,295 bp [1] End 77,640,069 bp [1]
Gene location (Mouse) Chr. Chromosome 5 (mouse) [2] Band 5|5 A3 Start 20,986,645 bp [2] End 21,055,911 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gonad epithelium of colon monocyte right lung tibial nerve Achilles tendon lower lobe of lung upper lobe of lung upper lobe of left lung subcutaneous adipose tissue Top expressed in tail of embryo Gonadal ridge left lung lobe genital tubercle stroma of bone marrow trigeminal ganglion abdominal wall otic placode hair follicle dermis More reference expression data BioGPS More reference expression data
Gene ontology Molecular function SH3 domain binding phosphatase activity protein binding hydrolase activity non-membrane spanning protein tyrosine phosphatase activity phosphoprotein phosphatase activity protein tyrosine phosphatase activity Cellular component podosome cell junction cell projection focal adhesion cytoplasm cytosol nucleoplasm Biological process tissue regeneration dephosphorylation protein dephosphorylation regulation of epidermal growth factor receptor signaling pathway peptidyl-tyrosine dephosphorylation cellular response to epidermal growth factor stimulus ERBB2 signaling pathway negative regulation of ERBB signaling pathway negative regulation of platelet-derived growth factor receptor-beta signaling pathway cellular response to cytokine stimulus Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5782 19248 Ensembl ENSG00000127947 ENSMUSG00000028771 UniProt Q05209 P35831 RefSeq (mRNA) NM_001131008 NM_001131009 NM_002835 NM_011203 NM_001356590 NM_001356591 NM_001356592 RefSeq (protein) NP_001124480 NP_001124481 NP_002826 NP_035333 NP_001343519 NP_001343520 NP_001343521 Location (UCSC) Chr 7: 77.54 – 77.64 Mb Chr 5: 20.99 – 21.06 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Tyrosine-protein phosphatase non-receptor type 12 is an enzyme that in humans is encoded by the PTPN12 gene. ^{[5] [6]}

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein–protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility. ^[6]

Interactions

PTPN12 has been shown to interact with BCAR1, ^{[7] [8] [9] [10]} Grb2, ^[11] PSTPIP1, ^[12] TGFB1I1, ^[13] Paxillin ^{[14] [15] [16]} and SHC1. ^{[17] [18]}

References

1. GRCh38: Ensembl release 89: ENSG00000127947 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000028771 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Takekawa M, Itoh F, Hinoda Y, Adachi M, Ariyama T, Inazawa J, Imai K, Yachi A (March 1994). "Chromosomal localization of the protein tyrosine phosphatase G1 gene and characterization of the aberrant transcripts in human colon cancer cells". FEBS Lett. 339 (3): 222–8. doi:10.1016/0014-5793(94)80420-6. PMID   7509295. S2CID   4885570.
6. "Entrez Gene: PTPN12 protein tyrosine phosphatase, non-receptor type 12".
7. Lin, Yi; Ceacareanu Alice Corina; Hassid Aviv (August 2003). "Nitric oxide-induced inhibition of aortic smooth muscle cell motility: role of PTP-PEST and adaptor proteins p130cas and Crk". Am. J. Physiol. Heart Circ. Physiol. 285 (2): H710–21. doi:10.1152/ajpheart.01127.2002. ISSN   0363-6135. PMID   12714323. S2CID   42587789.
8. Garton, A J; Burnham M R; Bouton A H; Tonks N K (August 1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition". Oncogene. 15 (8): 877–85. doi: 10.1038/sj.onc.1201279 . ISSN   0950-9232. PMID   9285683.
9. Côté, J F; Charest A; Wagner J; Tremblay M L (September 1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry. 37 (38): 13128–37. doi:10.1021/bi981259l. ISSN   0006-2960. PMID   9748319.
10. Garton, A J; Flint A J; Tonks N K (November 1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST". Mol. Cell. Biol. 16 (11): 6408–18. doi:10.1128/MCB.16.11.6408. ISSN   0270-7306. PMC   231642 . PMID   8887669.
11. Charest, A; Wagner J; Kwan M; Tremblay M L (April 1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2". Oncogene. 14 (14): 1643–51. doi: 10.1038/sj.onc.1201008 . ISSN   0950-9232. PMID   9135065.
12. Dowbenko, D; Spencer S; Quan C; Lasky L A (January 1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein". J. Biol. Chem. 273 (2): 989–96. doi: 10.1074/jbc.273.2.989 . ISSN   0021-9258. PMID   9422760.
13. Nishiya, N; Iwabuchi Y; Shibanuma M; Côté J F; Tremblay M L; Nose K (April 1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain". J. Biol. Chem. 274 (14): 9847–53. doi: 10.1074/jbc.274.14.9847 . ISSN   0021-9258. PMID   10092676.
14. Shen, Y; Lyons P; Cooley M; Davidson D; Veillette A; Salgia R; Griffin J D; Schaller M D (January 2000). "The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo". J. Biol. Chem. 275 (2): 1405–13. doi: 10.1074/jbc.275.2.1405 . ISSN   0021-9258. PMID   10625692.
15. Côté, J F; Turner C E; Tremblay M L (July 1999). "Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST". J. Biol. Chem. 274 (29): 20550–60. doi: 10.1074/jbc.274.29.20550 . ISSN   0021-9258. PMID   10400685.
16. Shen, Y; Schneider G; Cloutier J F; Veillette A; Schaller M D (March 1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". J. Biol. Chem. 273 (11): 6474–81. doi: 10.1074/jbc.273.11.6474 . ISSN   0021-9258. PMID   9497381.
17. Habib, T; Herrera R; Decker S J (October 1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase". J. Biol. Chem. 269 (41): 25243–6. doi: 10.1016/S0021-9258(18)47237-7 . ISSN   0021-9258. PMID   7929214.
18. Charest, A; Wagner J; Jacob S; McGlade C J; Tremblay M L (April 1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity". J. Biol. Chem. 271 (14): 8424–9. doi: 10.1074/jbc.271.14.8424 . ISSN   0021-9258. PMID   8626541.

Further reading

• Takekawa M, Itoh F, Hinoda Y, et al. (1993). "Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein-tyrosine-phosphatase". Biochem. Biophys. Res. Commun. 189 (2): 1223–30. doi:10.1016/0006-291X(92)92335-U. PMID   1472029.
• Yi T, Cleveland JL, Ihle JN (1991). "Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification". Blood. 78 (9): 2222–8. doi: 10.1182/blood.V78.9.2222.2222 . PMID   1932742.
• Garton AJ, Tonks NK (1994). "PTP-PEST: a protein tyrosine phosphatase regulated by serine phosphorylation". EMBO J. 13 (16): 3763–71. doi:10.1002/j.1460-2075.1994.tb06687.x. PMC   395289 . PMID   7520867.
• Habib T, Herrera R, Decker SJ (1994). "Activators of protein kinase C stimulate association of Shc and the PEST tyrosine phosphatase". J. Biol. Chem. 269 (41): 25243–6. doi: 10.1016/S0021-9258(18)47237-7 . PMID   7929214.
• Yang Q, Co D, Sommercorn J, Tonks NK (1993). "Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase". J. Biol. Chem. 268 (23): 17650. doi: 10.1016/S0021-9258(19)85383-8 . PMID   8349645.
• Yang Q, Co D, Sommercorn J, Tonks NK (1993). "Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase". J. Biol. Chem. 268 (9): 6622–8. doi: 10.1016/S0021-9258(18)53296-8 . PMID   8454633.
• Charest A, Wagner J, Jacob S, et al. (1996). "Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity". J. Biol. Chem. 271 (14): 8424–9. doi: 10.1074/jbc.271.14.8424 . PMID   8626541.
• Garton AJ, Flint AJ, Tonks NK (1996). "Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST". Mol. Cell. Biol. 16 (11): 6408–18. doi:10.1128/MCB.16.11.6408. PMC   231642 . PMID   8887669.
• Charest A, Wagner J, Kwan M, Tremblay ML (1997). "Coupling of the murine protein tyrosine phosphatase PEST to the epidermal growth factor (EGF) receptor through a Src homology 3 (SH3) domain-mediated association with Grb2". Oncogene. 14 (14): 1643–51. doi: 10.1038/sj.onc.1201008 . PMID   9135065.
• Garton AJ, Burnham MR, Bouton AH, Tonks NK (1997). "Association of PTP-PEST with the SH3 domain of p130cas; a novel mechanism of protein tyrosine phosphatase substrate recognition". Oncogene. 15 (8): 877–85. doi: 10.1038/sj.onc.1201279 . PMID   9285683.
• Dowbenko D, Spencer S, Quan C, Lasky LA (1998). "Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein". J. Biol. Chem. 273 (2): 989–96. doi: 10.1074/jbc.273.2.989 . PMID   9422760.
• Shen Y, Schneider G, Cloutier JF, et al. (1998). "Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin". J. Biol. Chem. 273 (11): 6474–81. doi: 10.1074/jbc.273.11.6474 . PMID   9497381.
• Côté JF, Charest A, Wagner J, Tremblay ML (1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry. 37 (38): 13128–37. doi:10.1021/bi981259l. PMID   9748319.
• Wu Y, Dowbenko D, Lasky LA (1998). "PSTPIP 2, a second tyrosine phosphorylated, cytoskeletal-associated protein that binds a PEST-type protein-tyrosine phosphatase". J. Biol. Chem. 273 (46): 30487–96. doi: 10.1074/jbc.273.46.30487 . PMID   9804817.
• Sanger Centre, The; Washington University Genome Sequencing Cente, The (1999). "Toward a complete human genome sequence". Genome Res. 8 (11): 1097–108. doi: 10.1101/gr.8.11.1097 . PMID   9847074.
• Li J, Nishizawa K, An W, et al. (1999). "A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion". EMBO J. 17 (24): 7320–36. doi:10.1093/emboj/17.24.7320. PMC   1171078 . PMID   9857189.
• Garton AJ, Tonks NK (1999). "Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST". J. Biol. Chem. 274 (6): 3811–8. doi: 10.1074/jbc.274.6.3811 . PMID   9920935.
• Angers-Loustau A, Côté JF, Charest A, et al. (1999). "Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts". J. Cell Biol. 144 (5): 1019–31. doi:10.1083/jcb.144.5.1019. PMC   2148201 . PMID   10085298.
• Nishiya N, Iwabuchi Y, Shibanuma M, et al. (1999). "Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain". J. Biol. Chem. 274 (14): 9847–53. doi: 10.1074/jbc.274.14.9847 . PMID   10092676.