2'-phosphotransferase

2'-phosphotransferase
Identifiers
EC no.	2.7.1.160
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

In enzymology, a 2'-phosphotransferase (EC 2.7.1.160) is an enzyme that catalyzes the chemical reaction

2'-phospho-[ligated tRNA] + NAD⁺${\displaystyle \rightleftharpoons }$ mature tRNA + ADP-ribose 1,2-phosphate + nicotinamide + H₂O

Thus, the two substrates of this enzyme are [[2'-phospho-[ligated tRNA]]] and NAD⁺, whereas its 4 products are mature tRNA, ADP-ribose 1'',2''-phosphate, nicotinamide, and H₂O.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is 2'-phospho-[ligated tRNA]:NAD+ phosphotransferase. Other names in common use include yeast 2'-phosphotransferase, Tpt1, Tpt1p, and 2'-phospho-tRNA:NAD+ phosphotransferase.

References

• Steiger MA, Kierzek R, Turner DH, Phizicky EM (2001). "Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog". Biochemistry. 40 (46): 14098–105. doi:10.1021/bi011388t. PMID   11705403.
• Spinelli SL, Kierzek R, Turner DH, Phizicky EM (1999). "Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast". J. Biol. Chem. 274 (5): 2637–44. doi: 10.1074/jbc.274.5.2637 . PMID   9915792.
• Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM (1997). "A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae". J. Biol. Chem. 272 (20): 13203–10. doi: 10.1074/jbc.272.20.13203 . PMID   9148937.
• McCraith SM, Phizicky EM (1991). "An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule". J. Biol. Chem. 266 (18): 11986–92. doi: 10.1016/S0021-9258(18)99054-X . PMID   2050693.
• Hu QD, Lu H, Huo K, Ying K, Li J, Xie Y, Mao Y, Li YY (2003). "A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis". Cell. Mol. Life Sci. 60 (8): 1725–32. doi:10.1007/s00018-003-3107-7. PMC   11138648 . PMID   14504659.
• Steiger MA, Jackman JE, Phizicky EM (2005). "Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism". RNA. 11 (1): 99–106. doi:10.1261/rna.7194605. PMC   1370695 . PMID   15611300.
• Sawaya R, Schwer B, Shuman S (2005). "Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1". RNA. 11 (1): 107–13. doi:10.1261/rna.7193705. PMC   1370696 . PMID   15611301.
• Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S (2005). "Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1". J. Mol. Biol. 348 (2): 295–305. doi:10.1016/j.jmb.2005.02.049. PMID   15811369.