FUT8

FUT8
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DE0
Identifiers
Aliases	FUT8 , fucosyltransferase 8, CDGF, CDGF1
External IDs	OMIM: 602589 MGI: 1858901 HomoloGene: 9650 GeneCards: FUT8
Gene location (Human) Chr. Chromosome 14 (human) [1] Band 14q23.3 Start 65,410,592 bp [1] End 65,744,121 bp [1]
Gene location (Mouse) Chr. Chromosome 12 (mouse) [2] Band 12|12 C3 Start 77,284,899 bp [2] End 77,523,112 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in corpus callosum olfactory bulb trigeminal ganglion inferior ganglion of vagus nerve pylorus subthalamic nucleus Achilles tendon ganglionic eminence pons spinal ganglia Top expressed in seminal vesicula sciatic nerve olfactory epithelium left colon Epithelium of stomach crypt of lieberkuhn of small intestine optic nerve olfactory tubercle cerebellar vermis Paneth cell More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity glycosyltransferase activity SH3 domain binding glycoprotein 6-alpha-L-fucosyltransferase activity alpha-(1->6)-fucosyltransferase activity Cellular component integral component of membrane Golgi apparatus membrane Golgi membrane Golgi cisterna membrane extracellular exosome cytosol Biological process GDP-L-fucose metabolic process regulation of cellular response to oxidative stress N-glycan fucosylation N-glycan processing in utero embryonic development respiratory gaseous exchange by respiratory system protein glycosylation oligosaccharide biosynthetic process regulation of gene expression integrin-mediated signaling pathway protein N-linked glycosylation transforming growth factor beta receptor signaling pathway cell migration L-fucose catabolic process receptor metabolic process protein N-linked glycosylation via asparagine Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 2530 53618 Ensembl ENSG00000033170 ENSMUSG00000021065 UniProt Q9BYC5 Q9WTS2 RefSeq (mRNA) NM_004480 NM_178154 NM_178155 NM_178156 NM_178157 NM_001371533 NM_001371534 NM_001371536 NM_001252614 NM_001252615 NM_001252616 NM_016893 RefSeq (protein) NP_004471 NP_835368 NP_835369 NP_001358462 NP_001358463 NP_001358465 NP_001239543 NP_001239544 NP_001239545 NP_058589 Location (UCSC) Chr 14: 65.41 – 65.74 Mb Chr 12: 77.28 – 77.52 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Alpha-(1,6)-fucosyltransferase is an enzyme that in humans is encoded by the FUT8 gene. ^{[5] [6]}

This enzyme belongs to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternatively spliced variants encoding different isoforms have been identified. ^[6]

Kyowa Hakko Kirin's "Potelligent" platform uses a CHO cell line in which FUT8 has been knocked out to make afucosylated monoclonal antibodies. ^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000033170 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000021065 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Costache M, Apoil PA, Cailleau A, Elmgren A, Larson G, Henry S, Blancher A, Iordachescu D, Oriol R, Mollicone R (Dec 1997). "Evolution of fucosyltransferase genes in vertebrates". J Biol Chem. 272 (47): 29721–8. doi: 10.1074/jbc.272.47.29721 . PMID   9368041.
6. "Entrez Gene: FUT8 fucosyltransferase 8 (alpha (1,6) fucosyltransferase)".
7. Yu, X; Marshall, MJE; Cragg, MS; Crispin, M (June 2017). "Improving Antibody-Based Cancer Therapeutics Through Glycan Engineering" (PDF). BioDrugs. 31 (3): 151–166. doi:10.1007/s40259-017-0223-8. PMID   28466278. S2CID   3722081.

Further reading

• Miyoshi E, Noda K, Yamaguchi Y, et al. (2000). "The alpha1-6-fucosyltransferase gene and its biological significance". Biochim. Biophys. Acta. 1473 (1): 9–20. doi:10.1016/s0304-4165(99)00166-x. PMID   10580126.
• Kalyanaraman VS, Rodriguez V, Veronese F, et al. (1990). "Characterization of the secreted, native gp120 and gp160 of the human immunodeficiency virus type 1". AIDS Res. Hum. Retroviruses. 6 (3): 371–80. doi:10.1089/aid.1990.6.371. PMID   2187500.
• Pal R, Hoke GM, Sarngadharan MG (1989). "Role of oligosaccharides in the processing and maturation of envelope glycoproteins of human immunodeficiency virus type 1". Proc. Natl. Acad. Sci. U.S.A. 86 (9): 3384–8. Bibcode:1989PNAS...86.3384P. doi: 10.1073/pnas.86.9.3384 . PMC   287137 . PMID   2541446.
• Dewar RL, Vasudevachari MB, Natarajan V, Salzman NP (1989). "Biosynthesis and processing of human immunodeficiency virus type 1 envelope glycoproteins: effects of monensin on glycosylation and transport". J. Virol. 63 (6): 2452–6. doi:10.1128/jvi.63.6.2452-2456.1989. PMC   250699 . PMID   2542563.
• Kozarsky K, Penman M, Basiripour L, et al. (1989). "Glycosylation and processing of the human immunodeficiency virus type 1 envelope protein". J. Acquir. Immune Defic. Syndr. 2 (2): 163–9. PMID   2649653.
• Robinson WE, Montefiori DC, Mitchell WM (1988). "Evidence that mannosyl residues are involved in human immunodeficiency virus type 1 (HIV-1) pathogenesis". AIDS Res. Hum. Retroviruses. 3 (3): 265–82. doi:10.1089/aid.1987.3.265. PMID   2829950.
• Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID   8619474.
• Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC   139146 . PMID   9110174.
• Yanagidani S, Uozumi N, Ihara Y, et al. (1997). "Purification and cDNA cloning of GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase (alpha1-6 FucT) from human gastric cancer MKN45 cells". J. Biochem. 121 (3): 626–32. doi:10.1093/oxfordjournals.jbchem.a021631. PMID   9133635.
• Yamaguchi Y, Fujii J, Inoue S, et al. (1999). "Mapping of the alpha-1,6-fucosyltransferase gene, FUT8, to human chromosome 14q24.3". Cytogenet. Cell Genet. 84 (1–2): 58–60. doi:10.1159/000015215. PMID   10343104. S2CID   85305954.
• Takahashi T, Ikeda Y, Tateishi A, et al. (2000). "A sequence motif involved in the donor substrate binding by alpha1,6-fucosyltransferase: the role of the conserved arginine residues". Glycobiology. 10 (5): 503–10. doi: 10.1093/glycob/10.5.503 . PMID   10764839.
• Yamaguchi Y, Ikeda Y, Takahashi T, et al. (2000). "Genomic structure and promoter analysis of the human alpha1, 6-fucosyltransferase gene (FUT8)". Glycobiology. 10 (6): 637–43. doi: 10.1093/glycob/10.6.637 . PMID   10814706.
• Takahashi T, Ikeda Y, Miyoshi E, et al. (2000). "alpha1,6fucosyltransferase is highly and specifically expressed in human ovarian serous adenocarcinomas". Int. J. Cancer. 88 (6): 914–9. doi:10.1002/1097-0215(20001215)88:6<914::AID-IJC12>3.0.CO;2-1. PMID   11093814.
• Roos C, Kolmer M, Mattila P, Renkonen R (2002). "Composition of Drosophila melanogaster proteome involved in fucosylated glycan metabolism". J. Biol. Chem. 277 (5): 3168–75. doi: 10.1074/jbc.M107927200 . PMID   11698403.
• Coullin P, Crooijmans RP, Groenen MA, et al. (2003). "Assignment of FUT8 to chicken chromosome band 5q1.4 and to human chromosome 14q23.2→q24.1 by in situ hybridization. Conserved and compared synteny between human and chicken". Cytogenet. Genome Res. 97 (3–4): 234–8. doi:10.1159/000066611. PMID   12438718. S2CID   27532607.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Martinez-Duncker I, Michalski JC, Bauvy C, et al. (2004). "Activity and tissue distribution of splice variants of alpha6-fucosyltransferase in human embryogenesis". Glycobiology. 14 (1): 13–25. doi: 10.1093/glycob/cwh006 . PMID   14514715.
• Ito Y, Miyauchi A, Yoshida H, et al. (2003). "Expression of alpha1,6-fucosyltransferase (FUT8) in papillary carcinoma of the thyroid: its linkage to biological aggressiveness and anaplastic transformation". Cancer Lett. 200 (2): 167–72. doi:10.1016/S0304-3835(03)00383-5. PMID   14568171.

External links

• Overview of all the structural information available in the PDB for UniProt : Q9BYC5 (Human FUT8) at the PDBe-KB .