TRNA(His) guanylyltransferase

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tRNA(His) guanylyltransferase
Identifiers
EC no. 2.7.7.79
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tRNA(His) guanylyltransferase (EC 2.7.7.79, histidine tRNA guanylyltransferase, Thg1p, Thg1) is an enzyme with systematic name p-tRNA(His):GTP guanylyltransferase (ATP-hydrolysing). [1] [2] [3] [4] [5] [6] This enzyme catalyses the following chemical reaction

p-tRNA(His) + ATP + GTP pppGp-tRNA(His) + AMP + diphosphate (overall reaction)
(1a) p-tRNA(His) + ATP App-tRNA(His) + diphosphate
(1b) App-tRNA(His) + GTP pppGp-tRNA(His) + AMP

The enzyme requires a divalent cation for activity.

Thg1 enzymes are the only nucleic acid polymerases known to be capable of catalyzing nucleotide addition in the 3′–5′ direction. [7]

References

  1. ↑ Jahn D, Pande S (December 1991). "Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. II. Catalytic mechanism". The Journal of Biological Chemistry. 266 (34): 22832–6. doi: 10.1016/S0021-9258(18)54429-X . PMID   1660462.
  2. ↑ Pande S, Jahn D, Söll D (December 1991). "Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and physical properties". The Journal of Biological Chemistry. 266 (34): 22826–31. doi: 10.1016/S0021-9258(18)54428-8 . PMID   1660461.
  3. ↑ Gu W, Jackman JE, Lohan AJ, Gray MW, Phizicky EM (December 2003). "tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis". Genes & Development. 17 (23): 2889–901. doi:10.1101/gad.1148603. PMC   289149 . PMID   14633974.
  4. ↑ Placido A, Sieber F, Gobert A, Gallerani R, Giegé P, Maréchal-Drouard L (November 2010). "Plant mitochondria use two pathways for the biogenesis of tRNAHis". Nucleic Acids Research. 38 (21): 7711–7. doi:10.1093/nar/gkq646. PMC   2995067 . PMID   20660484.
  5. ↑ Jackman JE, Phizicky EM (April 2008). "Identification of critical residues for G-1 addition and substrate recognition by tRNA(His) guanylyltransferase". Biochemistry. 47 (16): 4817–25. doi:10.1021/bi702517q. PMID   18366186.
  6. ↑ Hyde SJ, Eckenroth BE, Smith BA, Eberley WA, Heintz NH, Jackman JE, Doublié S (November 2010). "tRNA(His) guanylyltransferase (THG1), a unique 3'-5' nucleotidyl transferase, shares unexpected structural homology with canonical 5'-3' DNA polymerases". Proceedings of the National Academy of Sciences of the United States of America. 107 (47): 20305–10. doi: 10.1073/pnas.1010436107 . PMC   2996709 . PMID   21059936.
  7. ↑ Jackman, Jane E.; Gott, Jonatha M.; Gray, Michael W. (May 2012). "Doing it in reverse: 3'-to-5' polymerization by the Thg1 superfamily". RNA. 18 (5): 886–899. doi:10.1261/rna.032300.112. ISSN   1469-9001. PMC   3334698 . PMID   22456265.
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