Zinc finger protein 226

Last updated
ZNF226
Identifiers
Aliases ZNF226 , zinc finger protein 226
External IDs MGI: 1929114 HomoloGene: 128597 GeneCards: ZNF226
Orthologs
SpeciesHumanMouse
Entrez

7769

56707

Ensembl

ENSG00000167380

ENSMUSG00000087598

UniProt

Q9NYT6

n/a

RefSeq (mRNA)

NM_019940
NM_001310670

RefSeq (protein)

n/a

Location (UCSC) Chr 19: 44.17 – 44.18 Mb Chr 7: 23.89 – 23.91 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse
Figure 1. Bird's Eye View of the ZNF226 Transcript and Promoters ZNF226 Gene.png
Figure 1. Bird's Eye View of the ZNF226 Transcript and Promoters
Figure 2. Human ZNF226 5' UTR Secondary Structure 5' UTR ZNF226.png
Figure 2. Human ZNF226 5’ UTR Secondary Structure
Figure 3. Human ZNF226 3' UTR Secondary Structure 3' UTR ZNF226.png
Figure 3. Human ZNF226 3' UTR Secondary Structure
Figure 4. A multiple sequence alignment of human ZNF226 and ortholog species with selected regions highlighted. ZNF226 Ortholog Multiple Sequence Alignment 1.png
Figure 4. A multiple sequence alignment of human ZNF226 and ortholog species with selected regions highlighted.

Zinc finger protein 226 is a protein that in humans is encoded by the ZNF226 gene. [5]

Gene

The zinc finger protein 226 is also known as the Kruppel-associated box protein. [6] Within humans, the ZNF226 gene is found on the plus strand of chromosome19q13, spanning 13,311 nucleotides from 44,165,070 to 44,178,381. [5]

Transcript

Currently, there are 20 different transcript variants encoding ZNF226. [5] All of them have six or seven identified exon regions within ZNF226. [7] The longest identified transcript, ZNF226 transcript variant x4 spans 2,797 base pairs (bp). [7]

Protein

ZNF226 is currently known to have three isoforms within humans: ZNF226 isoform X1, ZNF226 isoform X2, and ZNF226 isoform X3. [5] The ZNF226 isoform X1 protein is the longest known variant, with 803 amino acids. [5] This protein contains the Kruppel associated box A (KRAB-A) domain, which functions as a transcriptional repressor. [8] However, the exact function of the ZNF226 protein is currently unknown. Within isoform X1, there are 18 C2H2 zinc finger structural motif (zf-C2H2) domains, which are known to bind either zinc ions (Zn2+) or nucleic acid (Figure 7–8). [9] [10] Within those regions, cysteine and histidine are the primary amino acids that bind to Zn2+ or nucleic acid, although other amino acids have been identified for binding (Figure 7–8). In addition to the KRAB-A domain and zf-C2H2 domains, there are zinc finger double domains which also contain binding sites for ions or nucleic acids. [7]

ZNF226 human and ortholog protein sequences have molecular weights between 89 and 92 kDa. [11] They had isoelectric points (pI) ranging from 8.60 to 9.00. [11] In humans, the zf-C2H2 and zinc finger double domain region of ZNF226 isoform X1 is 59.3 kDa with a theoretical pI of 9.11. [11] With the spacing of cysteine, or C, there is a cysteine every three amino acids. At least one of the amino acids in between the C's are either aspartic acid or glutamic acid. [12] Despite the region's patterns of aspartic acid, it is still considered to have a lesser amount of the amino acid at 1.9%. [12] There is also repetition within the chemical patterns within humans that is characteristic of ZNF226. [12] These repetitions appear most common within the zf-C2H2 and zinc finger double domains of the protein, notably with cysteine and histidine binding sites. [12] Predicted secondary structures of ZNF226 demonstrate a variable number of alpha helices, beta-stranded bridges, and random coils throughout the protein. Using various programs, such as GOR4 and the Chou and Fasman program, there is overall similarity in the predictions of coiled, stranded, and helix regions throughout the protein. [13] [14] [15]

Regulation

Gene

Promoter

Using the Genomatix software, GXP_7536741 (1142 bp) was identified as the best promoter of ZNF226 (Figure 1). [16] Within the last 500 bp of the promoter, the signal transducer and activator of transcription (V$STAT.01), selenocysteine tRNA activating factor (V$THAP11.01), and cell cycle regulators: cell cycle homology region (V$CHR.01) were conserved among Homo sapiens, Macaca mulatta, Pan troglodytes, and Canis lupus familiaris. [16] In addition, the SPI-1 proto-oncogene; hematopoietic TF PU.1 (V$SPI1.02) was also known for binding to a promoter region within the c-fes proto-oncogene which encodes tyrosine kinase. [17] The TF binding site is also found in two regions within the promoter sequence. The signal transducer and activator of transcription binding site was also conserved in two regions, and is known to have a higher binding specificity. [18] The selenocysteine tRNA activating factor plays a role in embryonic stem cell regeneration. [19] The cell cycle regulators: cell cycle homology region binding site plays an important role in cell survival, where mutations in the transcription factor can lead to apoptosis. [20]

Figure 5. A multiple sequence alignment of human ZNF226 and ortholog species with selected regions highlighted. ZNF226 Ortholog Multiple Sequence Alignment 2.png
Figure 5. A multiple sequence alignment of human ZNF226 and ortholog species with selected regions highlighted.
Figure 6. The ZNF226 evolutionary rate in comparison to the cytochrome c and fibrinogen alpha chain proteins ZNF226 Evolutionary Graph.png
Figure 6. The ZNF226 evolutionary rate in comparison to the cytochrome c and fibrinogen alpha chain proteins
Figure 7. ZNF226 zf-C2H2 structural domains highlighted on the conceptual translation ZNF226 Conceptual Translation 1.1.png
Figure 7. ZNF226 zf-C2H2 structural domains highlighted on the conceptual translation
Figure 8. ZNF226 zf-C2H2 structural domains highlighted on the conceptual translation ZNF226 Conceptual 1.2.png
Figure 8. ZNF226 zf-C2H2 structural domains highlighted on the conceptual translation
Figure 9. Sequence logo of ZNF226 zf-C2H2 structural domain DNA binding sites ZNF226 zf-C2H2 Domains Binding Sites.png
Figure 9. Sequence logo of ZNF226 zf-C2H2 structural domain DNA binding sites

Tissue distribution

In terms of gene expression, ZNF226 is generally expressed in most tissues. [5] Microarray data illustrates higher expression of ZNF226 within the ovaries. [21] This is further supported by data which depicts a decrease in ZNF226 expression in granulosa cells within individuals with polycystic ovary syndrome. [22] There was also higher expressions of ZNF226 observed within the thyroid compared to other tissues. [5] Evidence of decreased ZNF226 expression is observed with individuals with papillary thyroid cancer. [23]

Within fetuses, there is some level of ZNF226 expression present within all tissues throughout the gestational period of 10 to 20 weeks. [5] However, there is a higher level of ZNF226 expression in the heart at 10 weeks of gestation, and a decreased level of expression within kidneys at 20 weeks gestation. [5]

ZNF226 expression has been observed within epithelial progenitor cells (EPCs) in the peripheral blood (PB) and umbilical cord blood (CB). The gene expression is lower in PB-EPCs when compared to CB-EPCs. [24] PB-EPCs have more tumor suppressor (TP53) expression when compared to CB-EPCs. [24] CB-EPCs have more angiogenic expression, or growth and splitting of vasculature. [24]

Transcript

Using RNAfold, minimum free energy structures were created based on the extended 5’ and 3’ untranslated region (UTR) in human sequences. Unconserved amino acids, miRNA, stem-loop formations, and RNA binding proteins (RBPs) are shown on the diagram (Figure 2–3).

miRNA targeting

Within the 5’ UTR region, both miR-4700-5p and miR-4667-5p were referenced in an experiment which identified certain miRNAs expressed consistently in ERBB2+ breast cancer gene. [25] In addition, miR-8089 was referenced in a study showing certain novel miRNAs found within sepsis patients. [26] miR-4271 was shown to have effects on coronary heart disease binding to the 3' UTR region of the APOC3 gene. [27] Literature on miR-7113-5p shows that this miRNA is a mirtron. [28]

Within the 3’ UTR region, miR-3143 is referenced in a study where miRNAs were expressed consistently in ERBB2+ breast cancer gene. [25] miR-152-5p plays a role in inhibiting DNA methylation of genes involved in metabolic and inflammatory pathways. [29] miR-31-3p is overexpressed in esophageal squamous cell carcinoma (ESCC). [30] One miRNA result, miR-150-5p, was conserved across multiple homologs within the 3’ UTR region more than 3000 bp downstream. [31] The miR-150-5p miRNA plays a role in colorectal cancer (CRC), where a lower expression of the miRNA was associated with a suppression of CRC metastasis. [31]

RNA binding proteins

In terms of some of the RBPs found, PAPBC1 had five binding sites, two of which are highlighted on the 5’ UTR. This protein is known to attach poly-a-tails for proteins that have entered the cytoplasm, preventing them from re-entry into the nucleus. The FUS protein was another one found with a binding site on a predicted stem loop. The gene encodes for a protein which facilitates transportation of the protein into the cytoplasm. [32] Within the 3’ UTR, the RBMY1A1, RBMX, and ACO1 proteins were some of the top scoring RBPs. The RBMY1A1 is a protein known to partake in splicing, and is required for sperm development. [33] The RBMX protein is a homolog of the RBMY protein involved in sperm production. [34] It is also known to promote transcription of a tumor suppressor gene, TXNIP. [34] ACO1 is another RBP known to bind with mRNA to regulate iron levels. [35] By binding to iron responsive elements, it can repress translation of ferritin and inhibit degradation of transferring receptor mRNA when iron levels become low. [35]

Protein

Analysis to predict post-translational modifications of the protein were conducted on. Based on the results of Expasy's Myristoylator in Homo sapiens, Mirounga leonina, and Fukomys damarensis , it can be concluded that ZNF226 is not myristoylated at the N-terminus. [36] Numerous predicted binding sites for post-translational modifications were also identified among the three species. The phosphorylation region at the C-terminus of the protein was also identified as a match for the protein kinase C phosphorylation binding site. [37] [38] [39] [40] S-nitrosylation was another identified modification at C354 (Figure 2). This modification is found in SRG1, a zinc finger protein that plays a role preventing nitric oxide (NO) synthesis. [41] When NO is sustained, s-nitrosylation occurs within the protein, disrupting its transcriptional repression abilities. [41] Acetylation was another modification identified. In the case of promyelocytic leukemia, a condition resulting in the abundance of blood forming cells in the bone marrow, promyelocytic leukemia zinc finger proteins are known to be activated by histone acetyltransferases, or by acetylation of a C-terminus lysine. [42] Acetylation in other zinc finger proteins, such as GATA1, are known to enhance their ability to interact with other proteins. [43] Arginine dimethylation is another identified modification within ZNF226. Arginine methylation of cellular nucleic acid binding protein (CNBP), a zinc finger protein, has shown to impede its ability to bind nucleic acids. [44]

It is predicted that ZNF226 localizes within the nucleus, which aligns with its known functions as a transcription factor. [45] It has also been predicted to localize within the mitochondria. [45]

Homology/evolution

Although there is little information available on the ZNF226 gene, homologs of the gene have been found across eukaryotes and bacteria species. Strict orthologs were only found within mammals (Figure 4). The ZNF226 gene is also closely related to the paralog ZNF234 in humans, and the Zfp111 gene within mice. [5] [8] Across the various species in which ZNF226 orthologs and homologs that were identified, conservation of the C2H2 binding sites is apparent (Figure 4–5). [12] In human ZNF226 paralogs, there is also conservation of the C2H2 binding sites, as well as nucleic acid binding sites. [5]

Slow rate evolution is apparent for the ZNF226 protein. It evolves in a manner similar to the cytochrome c protein instead of the fibrinogen alpha chain protein (Figure 6).

Interacting Proteins

Two interactions detected via the two hybrid method occurred with SSBP3 and ATF4, both of which are transcription factors.

ATF4/CREB-2 is a transcription factor which binds to the long terminal repeat of the human T-cell leukemia type1 virus (HTLV-1). It can be an activator of HTLV-1. [46] [47] [48]

SSBP3/CSDP is found in mice embryonic stem cells to develop into trophoblasts (provide nutrients to embryo). [46] [49] ZNF226 is expressed at greater levels within human stem cells. [24]

Function

With ZNF226 being a transcription factor, playing a role in transcriptional repression, the 18 zf-C2H2 binding domains are predicted to bind to the DNA sequence shown in the sequence logo (Figure 7–9). [9] [10]

Clinical significance

Associated diseases and conditions

A mutation within ZNF226 gene has been positively correlated with the presence of hepatocellular carcinoma (HCC). [50] A particular SNP (rs2927438) also correlated with an increased expression of ZNF226 in brain frontal cortical tissue and peripheral mononuclear cells, such as T cells and B cells. [51] The promoter region of ZNF226 was found to be hypomethylated in those who were exposed to the Chinese famine. [52] The hypomethylated region in ZNF226 was shown to have a correlation of methylation in the blood and the prefrontal cortex, although the exact function of the protein in the famine is not understood. [52] ZNF226 gene was listed among many other genes with a copy number variation (CNV) that was associated with single common variable immunodeficiency (CVID).

SNPs

Numerous SNPs were identified throughout the ZNF226 gene. Within the GXP_7536741 promoter, there were two SNPs of interest that were found. Listed below are associated transcription factors for both SNPs.

MatrixMatrix Full NamePosition StartPosition EndStrandMatrix ScoreFunction
V$LHX4.01LIM homeobox 4, Gsh45880(+)0.849 Homeodomains play a role in vertebrate development. LIM domains contains two double zinc finger motifs and cysteine-rich regions. Plays a role in neural and lymphoid cell development. [53] [54]
V$BRN2.04POU class 3 homeobox 2 (POU3F2), OTF76179(-)0.843The POU domain has been shown to play a role in neuroendocrine development. [53] [55]
V$GSH2.01Homeodomain transcription factor Gsh-26179(+)0.966Gsh-2 is found to play a role in brain development. [56]
V$LBX2.01Ladybird homeobox 26179(-)0.893In mice, this TF was shown to play a role in testis and epididymis. [57]
V$CDP.02Transcriptional repressor CDP149171(-)0.954CDP has been studied for its role in regulating the S phase of the cell cycle. [58]
V$HOXC13.01Homeodomain transcription factor HOXC13 154170(-)0.917 HOXC13 plays a role in regulating keratin expression. [59]
V$NFY.04Nuclear factor Y (Y-box binding factor)155169(-)0.938Plays a role in cell cycle regulation, and binds to the CCAAT box upstream to transcription site. [60]

Related Research Articles

<span class="mw-page-title-main">Transcription factor</span> Protein that regulates the rate of DNA transcription

In molecular biology, a transcription factor (TF) is a protein that controls the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific DNA sequence. The function of TFs is to regulate—turn on and off—genes in order to make sure that they are expressed in the desired cells at the right time and in the right amount throughout the life of the cell and the organism. Groups of TFs function in a coordinated fashion to direct cell division, cell growth, and cell death throughout life; cell migration and organization during embryonic development; and intermittently in response to signals from outside the cell, such as a hormone. There are 1500-1600 TFs in the human genome. Transcription factors are members of the proteome as well as regulome.

<span class="mw-page-title-main">Gene expression</span> Conversion of a genes sequence into a mature gene product or products

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, and ultimately affect a phenotype. These products are often proteins, but in non-protein-coding genes such as transfer RNA (tRNA) and small nuclear RNA (snRNA), the product is a functional non-coding RNA. The process of gene expression is used by all known life—eukaryotes, prokaryotes, and utilized by viruses—to generate the macromolecular machinery for life.

<span class="mw-page-title-main">Zinc finger</span> Small structural protein motif found mostly in transcriptional proteins

A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn2+) which stabilizes the fold. It was originally coined to describe the finger-like appearance of a hypothesized structure from the African clawed frog (Xenopus laevis) transcription factor IIIA. However, it has been found to encompass a wide variety of differing protein structures in eukaryotic cells. Xenopus laevis TFIIIA was originally demonstrated to contain zinc and require the metal for function in 1983, the first such reported zinc requirement for a gene regulatory protein followed soon thereafter by the Krüppel factor in Drosophila. It often appears as a metal-binding domain in multi-domain proteins.

<span class="mw-page-title-main">Regulation of gene expression</span> Modifying mechanisms used by cells to increase or decrease the production of specific gene products

Regulation of gene expression, or gene regulation, includes a wide range of mechanisms that are used by cells to increase or decrease the production of specific gene products. Sophisticated programs of gene expression are widely observed in biology, for example to trigger developmental pathways, respond to environmental stimuli, or adapt to new food sources. Virtually any step of gene expression can be modulated, from transcriptional initiation, to RNA processing, and to the post-translational modification of a protein. Often, one gene regulator controls another, and so on, in a gene regulatory network.

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.

<span class="mw-page-title-main">CTCF</span> Transcription factor

Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, V(D)J recombination and regulation of chromatin architecture.

<span class="mw-page-title-main">Y box binding protein 1</span> Protein-coding gene in the species Homo sapiens

Y box binding protein 1 also known as Y-box transcription factor or nuclease-sensitive element-binding protein 1 is a protein that in humans is encoded by the YBX1 gene.

<span class="mw-page-title-main">HIVEP1</span> Protein-coding gene in the species Homo sapiens

Zinc finger protein 40 is a protein that in humans is encoded by the HIVEP1 gene.

<span class="mw-page-title-main">REPIN1</span> Protein-coding gene in the species Homo sapiens

Replication initiator 1 is a protein that in humans is encoded by the REPIN1 gene. The protein helps enable RNA binding activity as a replication initiation-region protein. The make up of REPIN 1 include three zinc finger hand clusters that organize polydactyl zinc finger proteins containing 15 zinc finger DNA- binding motifs. It has also been predicted to help in regulation of transcription via RNA polymerase II with it being located in the nucleoplasm. Expression of this protein has been seen in the colon, spleen, kidney, and 23 other tissues within the human body throughout.

<span class="mw-page-title-main">ZNF10</span> Protein-coding gene in the species Homo sapiens

Zinc finger protein 10 is a protein that in humans is encoded by the ZNF10 gene.

<span class="mw-page-title-main">ZNF143</span> Protein-coding gene

Zinc finger protein 143 is a protein that in humans is encoded by the ZNF143 gene.

<span class="mw-page-title-main">ZNF366</span> Protein-coding gene in the species Homo sapiens

Zinc finger protein 366, also known as DC-SCRIPT, is a protein that in humans is encoded by the ZNF366 gene. The ZNF366 gene was first identified in a DNA comparison study between 85 kb of Fugu rubripes sequence containing 17 genes with its homologous loci in the human draft genome.

<span class="mw-page-title-main">HIVEP3</span> Protein-coding gene in the species Homo sapiens

Transcription factor HIVEP3 is a protein that in humans is encoded by the HIVEP3 gene.

<span class="mw-page-title-main">ZNF300</span> Human protein-coding gene

Zinc finger protein 300 is a protein that in humans is encoded by the ZNF300 gene. The protein encoded by this gene is a C2H2-type zinc finger DNA binding protein and a likely transcription factor.

In molecular biology, the BEN domain is a protein domain which is found in diverse proteins including:

<span class="mw-page-title-main">Zinc finger protein 684</span> Protein found in humans

Zinc finger protein 684 is a protein that in humans is encoded by the ZNF684 gene.

LOC101928193 is a protein which in humans is encoded by the LOC101928193 gene. There are no known aliases for this gene or protein. Similar copies of this gene, called orthologs, are known to exist in several different species across mammals, amphibians, fish, mollusks, cnidarians, fungi, and bacteria. The human LOC101928193 gene is located on the long (q) arm of chromosome 9 with a cytogenic location at 9q34.2. The molecular location of the gene is from base pair 133,189,767 to base pair 133,192,979 on chromosome 9 for an mRNA length of 3213 nucleotides. The gene and protein are not yet well understood by the scientific community, but there is data on its genetic makeup and expression. The LOC101928193 protein is targeted for the cytoplasm and has the highest level of expression in the thyroid, ovary, skin, and testes in humans.

<span class="mw-page-title-main">ZNF548</span> Protein-coding gene in the species Homo sapiens

Zinc Finger Protein 548 (ZNF548) is a human protein encoded by the ZNF548 gene which is located on chromosome 19. It is found in the nucleus and is hypothesized to play a role in the regulation of transcription by RNA Polymerase II. It belongs to the Krüppel C2H2-type zinc-finger protein family as it contains many zinc-finger repeats.

<span class="mw-page-title-main">ZNF839</span> Protein which in humans is encoded by the ZNF839 gene

ZNF839 or zinc finger protein 839 is a protein which in humans is encoded by the ZNF839 gene. It is located on the long arm of chromosome 14. Zinc finger protein 839 is speculated to play a role in humoral immune response to cancer as a renal carcinoma antigen (NY-REN-50). This is because NY-REN-50 was found to be over expressed in cancer patients, especially those with renal carcinoma. Zinc finger protein 839 also plays a role in transcription regulation by metal-ion binding since it binds to DNA via C2H2-type zinc finger repeats.

<span class="mw-page-title-main">ZFP62</span> Gene in Humans

Zinc Finger Protein 62, also known as "ZNF62," "ZNF755," or "ZET," is a protein that in humans is encoded by the ZFP62 gene. ZFP62 is part of the C2H2 Zinc Finger family of genes.

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