6G-fructosyltransferase

6G-fructosyltransferase
Identifiers
EC no.	2.4.1.243
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

In enzymology, a 6G-fructosyltransferase (EC 2.4.1.243) is an enzyme that catalyzes the chemical reaction

[1-beta-D-fructofuranosyl-(2->1)-]m⁺1 alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n⁺1 alpha-D-glucopyranoside ${\displaystyle \rightleftharpoons }$ [1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n⁺1 beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >${\displaystyle \rightleftharpoons }$ 0)

Thus, the two substrates of this enzyme are [[[1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside]] and [[[1-beta-D-fructofuranosyl-(2->1)-]n+1 alpha-D-glucopyranoside]], whereas its 4 products are [[[1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside]], [[[1-beta-D-fructofuranosyl-(2->1)-]n+1]], [[beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >=]], and 0.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is 1F-oligo[beta-D-fructofuranosyl-(2->1)-]sucrose 6G-beta-D-fructotransferase. Other names in common use include fructan:fructan 6G-fructosyltransferase, 1F(1-beta-D-fructofuranosyl)m, sucrose:1F(1-beta-D-fructofuranosyl)_nsucrose, 6G-fructosyltransferase, 6G-FFT, 6G-FT, and 6G-fructotransferase.

References

• Shiomi N (1981). "Purification and characterisation of 6G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.)". Carbohydr. Res. 96 (2): 281–292. doi:10.1016/S0008-6215(00)81878-9.
• Shiomi N (1982). "Reverse reaction of fructosyl transfer catalysed by asparagus 6G-fructosyltransferase". Carbohydr. Res. 106: 166–169. doi:10.1016/S0008-6215(00)80743-0.
• Shiomi N, Ueno K (2004). "Cloning and expression of genes encoding fructosyltransferases from higher plants in food technology". J. Appl. Glycosci. 51 (2): 177–183. doi: 10.5458/jag.51.177 .
• Ueno K, Onodera S, Kawakami A, Yoshida M, Shiomi N (March 2005). "Molecular characterization and expression of a cDNA encoding fructan:fructan 6G-fructosyltransferase from asparagus (Asparagus officinalis)". The New Phytologist. 165 (3): 813–24. doi: 10.1111/j.1469-8137.2004.01294.x . PMID   15720693.