CCA tRNA nucleotidyltransferase

CCA tRNA nucleotidyltransferase
Identifiers
EC no.	2.7.7.72
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

CCA tRNA nucleotidyltransferase (EC 2.7.7.72, CCA-adding enzyme, tRNA adenylyltransferase, tRNA CCA-pyrophosphorylase, tRNA-nucleotidyltransferase, transfer-RNA nucleotidyltransferase, transfer ribonucleic acid nucleotidyl transferase, CTP(ATP):tRNA nucleotidyltransferase, transfer ribonucleate adenylyltransferase, transfer ribonucleate adenyltransferase, transfer RNA adenylyltransferase, transfer ribonucleate nucleotidyltransferase, ATP (CTP):tRNA nucleotidyltransferase, ribonucleic cytidylic cytidylic adenylic pyrophosphorylase, transfer ribonucleic adenylyl (cytidylyl) transferase, transfer ribonucleic-terminal trinucleotide nucleotidyltransferase, transfer ribonucleate cytidylyltransferase, ribonucleic cytidylyltransferase, -C-C-A pyrophosphorylase, ATP(CTP)-tRNA nucleotidyltransferase, tRNA adenylyl(cytidylyl)transferase, CTP:tRNA cytidylyltransferase) is an enzyme with systematic name CTP,CTP,ATP:tRNA cytidylyl,cytidylyl,adenylyltransferase. ^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

a tRNA precursor + 2 CTP + ATP ${\displaystyle \rightleftharpoons }$ a tRNA with a 3' CCA end + 3 diphosphate (overall reaction)

(1a) a tRNA precursor + CTP ${\displaystyle \rightleftharpoons }$ a tRNA with a 3' cytidine end + diphosphate

(1b) a tRNA with a 3' cytidine + CTP ${\displaystyle \rightleftharpoons }$ a tRNA with a 3' CC end + diphosphate

(1c) a tRNA with a 3' CC end + ATP ${\displaystyle \rightleftharpoons }$ a tRNA with a 3' CCA end + diphosphate

The acylation of all tRNAs with an amino acid occurs at the terminal ribose of a 3' CCA sequence.

See also

• TRNT1

References

1. Schofield P, Williams KR (August 1977). "Purification and some properties of Escherichia coli tRNA nucleotidyltransferase". The Journal of Biological Chemistry. 252 (15): 5584–8. doi: 10.1016/S0021-9258(19)63390-9 . PMID   328503.
2. Shi PY, Maizels N, Weiner AM (June 1998). "CCA addition by tRNA nucleotidyltransferase: polymerization without translocation?". The EMBO Journal. 17 (11): 3197–206. doi:10.1093/emboj/17.11.3197. PMC   1170658 . PMID   9606201.
3. Augustin MA, Reichert AS, Betat H, Huber R, Mörl M, Steegborn C (May 2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". Journal of Molecular Biology. 328 (5): 985–94. doi: 10.1016/S0022-2836(03)00381-4 . PMID   12729736.
4. Yakunin AF, Proudfoot M, Kuznetsova E, Savchenko A, Brown G, Arrowsmith CH, Edwards AM (August 2004). "The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and phosphatase activities". The Journal of Biological Chemistry. 279 (35): 36819–27. doi: 10.1074/jbc.M405120200 . PMID   15210699.
5. Hou YM (April 2010). "CCA addition to tRNA: implications for tRNA quality control". IUBMB Life. 62 (4): 251–60. doi:10.1002/iub.301. PMC   2848691 . PMID   20101632.

External links

• CCA+tRNA+nucleotidyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)