"Candidatus Epulopiscium" | |
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Genus: | "Candidatus Epulopiscium" corrig. Montgomery and Pollak 1988 |
Binomial name | |
"Candidatus Epulopiscium fishelsonii" corrig. Montgomery and Pollak 1988 | |
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USNM 40601 | |
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Candidatus Epulopiscium is a genus of Gram-positive bacteria that have a symbiotic relationship with surgeonfish. These bacteria are known for their unusually large size, many ranging from 0.2 - 0.7 mm (200–700 μm) in length. Until the discovery of Thiomargarita namibiensis in 1999, Epulonipiscium species were thought to be the largest bacteria. [1] [2] They are still the largest known heterotrophic bacteria.
In addition to their large size, Epulonipiscium, commonly referred to as "epulos," are morphologically diverse and extremely polyploid. [3] Epulos also have unique reproductive strategies in which certain cells can form intracellular offspring, similar to microbial sporulation; furthermore, several epulo morphologies exhibit sporulation.
While the bacteria have not been successfully grown in the lab, scientists have gained a better understanding of Epulonipiscium through microscopic, phylogenetic, and genomic analyses.
Epulonipiscium means "a guest at a banquet of fish" in Latin, from epulo ("guest at a feast" or "guest at a banquet") and piscium ("of a fish"), [4] as the organism was found inside the gut of marine surgeonfish. Epulonipiscium cells were initially classified as protists on the basis of their large size and unusual ultrastructure.
Originally, Epulonipiscium populations were thought to be a single species and given the name Epulopiscium fishelsoni in 1988, by Montgomery (one of the co-discovers) and Pollak. The epithet fishelsoni honors Lev Fishelson, a Polish-born Israeli ichthyologist [5] [6] who was part of the group that made the discovery while studying the intestines of a brown surgeonfish from the Red Sea in 1985. [7]
Later, however, Epulopiscium fishelsoni was shown to comprise two phylogenetically distinct groups of bacteria by Angert and collaborators using rRNA gene sequence comparisons. [1] Subsequent studies illustrated the relationship between these symbionts and the host surgeonfish.
The largest Epulonipiscium cells can be seen with the naked eye. However, because of their size, Epulonipiscium cells must compensate for their small surface-to-volume ratio, compared to other bacteria. One distinct feature is the cell membrane, which contains many folds to increase the effective surface area.
Additionally, Epulonipiscium cells are extremely polyploid, with individuals containing hundreds of thousands of copies of the genome. Since bacteria rely on diffusion rather than cytoskeletal transport as in eukaryotes, this extreme polyploidy allows for the production of gene products at numerous sites in the cell to produce biomolecules where they are needed.
The largest Epulonipiscium morphologies exhibit a unique viviparous reproduction. This unusual and derived form of sporulation produces anywhere from one to twelve daughter cells that grow inside of the parent cell, until the parent eventually lyses, and dies. [9] [10] These cells appear to not use binary fission for reproduction. Some morphologies use endospore formation for reproduction. [11] However, there are some smaller morphologies that reproduce through binary fission and spore formation.
Although sporulation is widespread among other bacteria (such as Bacillus subtilis and Clostridium species) in the phylum Bacillota, spore formation is usually brought about by overcrowding, the accumulation of toxins in the environment, or starvation, rather than a standard form of reproduction. The production of multiple endospores has been observed in other large gut symbionts such as Metabacterium polyspora , which are phylogenetically related to Epulonipiscium. [2] Since sporulation affords bacteria much more protection from the outside environment than binary fission, it is thought that the evolution of this unusual life cycle may assist transfer of the bacteria from one host to another.
Epulonipiscium species and their surgeonfish hosts are suggested to have a nutritional symbiotic relationship: Epulonipiscium species have only been found in surgeonfish that eat algae and detritus. It is suggested that Epulonipiscium species assist in the fish's digestion. [12] However, scientists have been unable to culture Epulonipiscium outside of its natural habitat.
An endosymbiont or endobiont is an organism that lives within the body or cells of another organism. Typically the two organisms are in a mutualistic relationship. Examples are nitrogen-fixing bacteria, which live in the root nodules of legumes, single-cell algae inside reef-building corals, and bacterial endosymbionts that provide essential nutrients to insects.
An endospore is a dormant, tough, and non-reproductive structure produced by some bacteria in the phylum Bacillota. The name "endospore" is suggestive of a spore or seed-like form, but it is not a true spore. It is a stripped-down, dormant form to which the bacterium can reduce itself. Endospore formation is usually triggered by a lack of nutrients, and usually occurs in gram-positive bacteria. In endospore formation, the bacterium divides within its cell wall, and one side then engulfs the other. Endospores enable bacteria to lie dormant for extended periods, even centuries. There are many reports of spores remaining viable over 10,000 years, and revival of spores millions of years old has been claimed. There is one report of viable spores of Bacillus marismortui in salt crystals approximately 25 million years old. When the environment becomes more favorable, the endospore can reactivate itself into a vegetative state. Most types of bacteria cannot change to the endospore form. Examples of bacterial species that can form endospores include Bacillus cereus, Bacillus anthracis, Bacillus thuringiensis, Clostridium botulinum, and Clostridium tetani. Endospore formation is not found among Archaea.
Thiomargarita namibiensis is a gram-negative, facultative anaerobic, coccoid bacterium found in South America's ocean sediments of the continental shelf of Namibia. The genus name Thiomargarita means "sulfur pearl." This refers to the cells' appearance as they contain microscopic elemental sulfur granules just below the cell wall that refract light creating a pearly iridescent luster. The cells are each covered in a mucus sheath aligned in a chain, resembling loose strings of pearls. The species name namibiensis means "of Namibia".
Acanthuridae are a family of ray-finned fish which includes surgeonfishes, tangs, and unicornfishes. The family includes about 86 extant species of marine fish living in tropical seas, usually around coral reefs. Many of the species are brightly colored and popular in aquaria.
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A bacterium, despite its simplicity, contains a well-developed cell structure which is responsible for some of its unique biological structures and pathogenicity. Many structural features are unique to bacteria and are not found among archaea or eukaryotes. Because of the simplicity of bacteria relative to larger organisms and the ease with which they can be manipulated experimentally, the cell structure of bacteria has been well studied, revealing many biochemical principles that have been subsequently applied to other organisms.
Trichonympha is a genus of single-celled, anaerobic parabasalids of the order Hypermastigia that is found exclusively in the hindgut of lower termites and wood roaches. Trichonympha’s bell shape and thousands of flagella make it an easily recognizable cell. The symbiosis between lower termites/wood roaches and Trichonympha is highly beneficial to both parties: Trichonympha helps its host digest cellulose and in return receives a constant supply of food and shelter. Trichonympha also has a variety of bacterial symbionts that are involved in sugar metabolism and nitrogen fixation.
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Bacteria are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit the air, soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria play a vital role in many stages of the nutrient cycle by recycling nutrients and the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in mutualistic, commensal and parasitic relationships with plants and animals. Most bacteria have not been characterised and there are many species that cannot be grown in the laboratory. The study of bacteria is known as bacteriology, a branch of microbiology.
Rhizopus microsporus is a fungal plant pathogen infecting maize, sunflower, and rice.
A prokaryote is a single-cell organism whose cell lacks a nucleus and other membrane-bound organelles. The word prokaryote comes from the Ancient Greek πρό (pró), meaning 'before', and κάρυον (káruon), meaning 'nut' or 'kernel'. In the two-empire system arising from the work of Édouard Chatton, prokaryotes were classified within the empire Prokaryota. However in the three-domain system, based upon molecular analysis, prokaryotes are divided into two domains: Bacteria and Archaea. Organisms with nuclei are placed in a third domain: Eukaryota.
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