PTK6

PTK6
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1RJA, 2KGT
Identifiers
Aliases	PTK6 , BRK, protein tyrosine kinase 6
External IDs	OMIM: 602004; MGI: 99683; HomoloGene: 68494; GeneCards: PTK6; OMA:PTK6 - orthologs
Gene location (Human) Chr. Chromosome 20 (human) [1] Band 20q13.33 Start 63,528,001 bp [1] End 63,537,376 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2 H4|2 103.62 cM Start 180,835,514 bp [2] End 180,844,582 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in mucosa of transverse colon human penis skin of abdomen skin of leg mucosa of pharynx gums amniotic fluid gingival epithelium oral cavity jejunal mucosa Top expressed in jejunum esophagus lip intestinal villus blastocyst skin of external ear tongue ileum Ileal epithelium duodenum More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding protein kinase activity non-membrane spanning protein tyrosine kinase activity kinase activity protein binding identical protein binding signaling receptor binding ATP binding protein tyrosine kinase activity Cellular component cytoplasm cell projection membrane ruffle extrinsic component of cytoplasmic side of plasma membrane nucleus nucleoplasm cytosol plasma membrane nuclear body Biological process cellular response to retinoic acid negative regulation of growth phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway protein phosphorylation negative regulation of protein tyrosine kinase activity regulation of cell population proliferation intestinal epithelial cell differentiation positive regulation of neuron projection development peptidyl-tyrosine autophosphorylation protein autophosphorylation innate immune response cell migration positive regulation of cell cycle ERBB2 signaling pathway negative regulation of signal transduction positive regulation of epidermal growth factor receptor signaling pathway tyrosine phosphorylation of STAT protein positive regulation of tyrosine phosphorylation of STAT protein cell differentiation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5753 20459 Ensembl ENSG00000101213 ENSMUSG00000038751 UniProt Q13882 Q64434 RefSeq (mRNA) NM_001256358 NM_005975 NM_009184 NM_001356304 RefSeq (protein) NP_001243287 NP_005966 NP_033210 NP_001343233 Location (UCSC) Chr 20: 63.53 – 63.54 Mb Chr 2: 180.84 – 180.84 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Tyrosine-protein kinase 6 is an enzyme that in humans is encoded by the PTK6 gene. ^{[5] [6] [7]}

Function

Tyrosine-protein kinase 6—also known as BRK ^[7] (breast tumor kinase)—is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. The encoded protein has been shown to undergo autophosphorylation. ^[7]

Clinical significance

Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. ^[7]

Interactions

PTK6 has been shown to interact with STAP2 ^[8] and KHDRBS1. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000101213 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000038751 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Lee ST, Strunk KM, Spritz RA (December 1993). "A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes". Oncogene. 8 (12): 3403–10. PMID   8247543.
6. Park SH, Lee KH, Kim H, Lee ST (September 1997). "Assignment of the human PTK6 gene encoding a non-receptor protein tyrosine kinase to 20q13.3 by fluorescence in situ hybridization". Cytogenet Cell Genet. 77 (3–4): 271–2. doi:10.1159/000134595. PMID   9284935.
7. "PTK6 protein tyrosine kinase 6". National Center for Biotechnology Information (NCBI). 4 June 2020.
8. Mitchell PJ, Sara E A, Crompton M R (August 2000). "A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK". Oncogene. 19 (37). ENGLAND: 4273–82. doi: 10.1038/sj.onc.1203775 . ISSN   0950-9232. PMID   10980601.
9. Derry JJ, Richard S, Valderrama Carvajal H, Ye X, Vasioukhin V, Cochrane A W, Chen T, Tyner A L (August 2000). "Sik (BRK) Phosphorylates Sam68 in the Nucleus and Negatively Regulates Its RNA Binding Ability". Mol. Cell. Biol. 20 (16). UNITED STATES: 6114–26. doi:10.1128/MCB.20.16.6114-6126.2000. ISSN   0270-7306. PMC   86087 . PMID   10913193.

Further reading

• Serfas MS, Tyner AL (2003). "Brk, Srm, Frk, and Src42A form a distinct family of intracellular Src-like tyrosine kinases". Oncol. Res. 13 (6–10): 409–19. doi: 10.3727/096504003108748438 . PMID   12725532.
• Mitchell PJ, Barker KT, Martindale JE, et al. (1994). "Cloning and characterisation of cDNAs encoding a novel non-receptor tyrosine kinase, brk, expressed in human breast tumours". Oncogene. 9 (8): 2383–90. PMID   8036022.
• Kamalati T, Jolin HE, Mitchell PJ, et al. (1997). "Brk, a breast tumor-derived non-receptor protein-tyrosine kinase, sensitizes mammary epithelial cells to epidermal growth factor". J. Biol. Chem. 271 (48): 30956–63. doi: 10.1074/jbc.271.48.30956 . PMID   8940083.
• Lee H, Kim M, Lee KH, et al. (1998). "Exon-intron structure of the human PTK6 gene demonstrates that PTK6 constitutes a distinct family of non-receptor tyrosine kinase". Mol. Cells. 8 (4): 401–7. doi: 10.1016/S1016-8478(23)13443-1 . PMID   9749526.
• Llor X, Serfas MS, Bie W, et al. (1999). "BRK/Sik expression in the gastrointestinal tract and in colon tumors". Clin. Cancer Res. 5 (7): 1767–77. PMID   10430081.
• Derry JJ, Richard S, Valderrama Carvajal H, et al. (2000). "Sik (BRK) Phosphorylates Sam68 in the Nucleus and Negatively Regulates Its RNA Binding Ability". Mol. Cell. Biol. 20 (16): 6114–26. doi:10.1128/MCB.20.16.6114-6126.2000. PMC   86087 . PMID   10913193.
• Mitchell PJ, Sara EA, Crompton MR (2000). "A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK". Oncogene. 19 (37): 4273–82. doi: 10.1038/sj.onc.1203775 . PMID   10980601.
• Kamalati T, Jolin HE, Fry MJ, Crompton MR (2000). "Expression of the BRK tyrosine kinase in mammary epithelial cells enhances the coupling of EGF signalling to PI 3-kinase and Akt, via erbB3 phosphorylation". Oncogene. 19 (48): 5471–6. doi: 10.1038/sj.onc.1203931 . PMID   11114724.
• Hong E, Shin J, Bang E, et al. (2001). "Complete sequence-specific 1H, 13C and 15N resonance assignments of the human PTK6 SH2 domain". J. Biomol. NMR. 19 (3): 291–2. doi:10.1023/A:1011221125013. PMID   11330822. S2CID   20836477.
• Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature. 414 (6866): 865–71. Bibcode:2001Natur.414..865D. doi: 10.1038/414865a . PMID   11780052.
• Kang KN, Kim M, Pae KM, Lee ST (2002). "Characterization of the 5'-flanking region of the human PTK6 gene". Biochim. Biophys. Acta. 1574 (3): 365–9. doi:10.1016/s0167-4781(02)00234-8. PMID   11997104.
• Qiu H, Miller WT (2002). "Regulation of the nonreceptor tyrosine kinase Brk by autophosphorylation and by autoinhibition". J. Biol. Chem. 277 (37): 34634–41. doi: 10.1074/jbc.M203877200 . PMID   12121988.
• Koo BK, Kim MH, Lee ST, Lee W (2002). "Purification and spectroscopic characterization of the human protein tyrosine kinase-6 SH3 domain". J. Biochem. Mol. Biol. 35 (3): 343–7. doi: 10.5483/BMBRep.2002.35.3.343 . PMID   12297019.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Coyle JH, Guzik BW, Bor YC, et al. (2003). "Sam68 Enhances the Cytoplasmic Utilization of Intron-Containing RNA and Is Functionally Regulated by the Nuclear Kinase Sik/BRK". Mol. Cell. Biol. 23 (1): 92–103. doi:10.1128/MCB.23.1.92-103.2003. PMC   140664 . PMID   12482964.
• Derry JJ, Prins GS, Ray V, Tyner AL (2003). "Altered localization and activity of the intracellular tyrosine kinase BRK/Sik in prostate tumor cells". Oncogene. 22 (27): 4212–20. doi: 10.1038/sj.onc.1206465 . PMID   12833144.
• Qiu H, Miller WT (2004). "Role of the Brk SH3 domain in substrate recognition". Oncogene. 23 (12): 2216–23. doi: 10.1038/sj.onc.1207339 . PMID   14676834.