EPH receptor A2

EPHA2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1MQB, 2E8N, 2K9Y, 2KSO, 2X10, 2X11, 3C8X, 3CZU, 3FL7, 3HEI, 3HPN, 3KKA, 3MBW, 3MX0, 3SKJ, 4P2K, 4PDO, 4TRL, 5EK7
Identifiers
Aliases	EPHA2 , Epha2, AW545284, Eck, Myk2, Sek-2, Sek2, ARCC2, CTPA, CTPP1, CTRCT6, EPH receptor A2, ECK
External IDs	OMIM: 176946; MGI: 95278; HomoloGene: 20929; GeneCards: EPHA2; OMA:EPHA2 - orthologs
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p36.13 Start 16,124,337 bp [1] End 16,156,069 bp [1]
Gene location (Mouse) Chr. Chromosome 4 (mouse) [2] Band 4 D3|4 73.67 cM Start 141,028,551 bp [2] End 141,056,695 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in vagina gallbladder skin of abdomen cervix epithelium salivary gland minor salivary glands trachea oral cavity gums parotid gland Top expressed in neural plate lip lens hair follicle jejunum duodenum esophagus right lung yolk sac mesoderm More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding protein kinase activity transferase activity kinase activity protein binding protein tyrosine kinase activity ATP binding ephrin receptor activity transmembrane receptor protein tyrosine kinase activity cadherin binding virus receptor activity transmembrane-ephrin receptor activity Cellular component integral component of membrane cell projection membrane ruffle membrane cell junction lamellipodium membrane leading edge membrane focal adhesion integral component of plasma membrane intracellular anatomical structure plasma membrane lamellipodium cell surface neuron projection receptor complex tight junctions Biological process regulation of lamellipodium assembly skeletal system development negative regulation of protein kinase B signaling cell differentiation regulation of cell adhesion mediated by integrin protein kinase B signaling activation of GTPase activity phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway keratinocyte differentiation vasculogenesis multicellular organism development protein phosphorylation cell adhesion blood vessel development notochord morphogenesis notochord cell development angiogenesis intrinsic apoptotic signaling pathway in response to DNA damage neural tube development neuron differentiation notochord regulation of ERK1 and ERK2 cascade cell chemotaxis post-anal tail morphogenesis axial mesoderm formation response to growth factor viral process cell migration apoptotic process peptidyl-tyrosine phosphorylation osteoclast differentiation osteoblast differentiation regulation of angiogenesis regulation of blood vessel endothelial cell migration ephrin receptor signaling pathway lens fiber cell morphogenesis mammary gland epithelial cell proliferation bone remodeling branching involved in mammary gland duct morphogenesis negative regulation of cytokine production defense response to Gram-positive bacterium negative regulation of lymphangiogenesis blood vessel endothelial cell proliferation involved in sprouting angiogenesis pericyte cell differentiation negative regulation of angiogenesis inflammatory response negative regulation of chemokine production blood vessel morphogenesis cAMP metabolic process cell motility viral entry into host cell positive regulation of protein localization to plasma membrane axon guidance protein localization to plasma membrane positive regulation of bicellular tight junction assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1969 13836 Ensembl ENSG00000142627 ENSMUSG00000006445 UniProt P29317 Q03145 RefSeq (mRNA) NM_004431 NM_001329090 NM_010139 RefSeq (protein) NP_001316019 NP_004422 NP_034269 Location (UCSC) Chr 1: 16.12 – 16.16 Mb Chr 4: 141.03 – 141.06 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

EPH receptor A2 (ephrin type-A receptor 2) is a protein that in humans is encoded by the EPHA2 gene. ^{[5] [6]}

Function

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. ^[6]

Clinical significance

It may be implicated in BRAF mutated melanomas becoming resistant to BRAF-inhibitors and MEK inhibitors. ^[7] It is also the receptor by which Kaposi's sarcoma-associated herpesvirus (KSHV) enters host cells; small molecule inhibitors of EphA2 have shown some ability to block KSHV entry into human cells. ^[8]

Interactions

EPH receptor A2 has been shown to interact with:

• Ephrin A1 ^[9]
• ACP1 ^[10]
• Grb2, ^[11]
• PIK3R1, ^[12] and
• SHC1. ^[11]

It was also shown that doxazosin is a small molecule agonist of EPH receptor A2. ^[13]

See also

• Vasculogenic mimicry

References

1. GRCh38: Ensembl release 89: ENSG00000142627 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000006445 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Sulman EP, Tang XX, Allen C, Biegel JA, Pleasure DE, Brodeur GM, Ikegaki N (April 1997). "ECK, a human EPH-related gene, maps to 1p36.1, a common region of alteration in human cancers". Genomics. 40 (2): 371–4. doi: 10.1006/geno.1996.4569 . PMID   9119409.
6. "Entrez Gene: EPHA2 EPH receptor A2".
7. "Counteracting Drug Resistance in Melanoma". 2015. Archived from the original on 2015-02-04. Retrieved 2015-02-04.
8. Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, König S, Ensser A, Myoung J, Brockmeyer NH, Stürzl M, Fleckenstein B, Neipel F (2012). "The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma–associated herpesvirus". Nat. Med. 18 (6): 961–6. doi:10.1038/nm.2805. PMC   3645317 . PMID   22635007.
9. Himanen JP, Goldgur Y, Miao H, Myshkin E, Guo H, Buck M, Nguyen M, Rajashankar KR, Wang BC, Nikolov DB (July 2009). "Ligand recognition by A-class Eph receptors: crystal structures of the EphA2 ligand-binding domain and the EphA2/ephrin-A1 complex". EMBO Rep. 10 (7): 722–8. doi: 10.1038/embor.2009.91 . PMC   2727437 . PMID   19525919.
10. Kikawa KD, Vidale DR, Van Etten RL, Kinch MS (October 2002). "Regulation of the EphA2 kinase by the low molecular weight tyrosine phosphatase induces transformation". J. Biol. Chem. 277 (42): 39274–9. doi: 10.1074/jbc.M207127200 . PMID   12167657.
11. Pratt RL, Kinch MS (October 2002). "Activation of the EphA2 tyrosine kinase stimulates the MAP/ERK kinase signaling cascade". Oncogene. 21 (50): 7690–9. doi:10.1038/sj.onc.1205758. PMID   12400011. S2CID   7083192.
12. Pandey A, Lazar DF, Saltiel AR, Dixit VM (December 1994). "Activation of the Eck receptor protein tyrosine kinase stimulates phosphatidylinositol 3-kinase activity". J. Biol. Chem. 269 (48): 30154–7. doi: 10.1016/S0021-9258(18)43790-8 . PMID   7982920.
13. Petty A, Myshkin E, Qin H, Guo H, Miao H, Tochtrop GP, Hsieh JT, Page P, Liu L, Lindner DJ, Acharya C, MacKerell AD, Ficker E, Song J, Wang BC (August 2012). "A small molecule agonist of EphA2 receptor tyrosine kinase inhibits tumor cell migration in vitro and prostate cancer metastasis in vivo". PLOS ONE. 7 (8): e42120. Bibcode:2012PLoSO...742120P. doi: 10.1371/journal.pone.0042120 . PMC   3419725 . PMID   22916121.

Further reading

• Flanagan JG, Vanderhaeghen P (1998). "The ephrins and Eph receptors in neural development". Annu. Rev. Neurosci. 21: 309–45. doi:10.1146/annurev.neuro.21.1.309. PMID   9530499. S2CID   1278600.
• Zhou R (1998). "The Eph family receptors and ligands". Pharmacol. Ther. 77 (3): 151–81. doi:10.1016/S0163-7258(97)00112-5. PMID   9576626.
• Holder N, Klein R (1999). "Eph receptors and ephrins: effectors of morphogenesis". Development. 126 (10): 2033–44. doi:10.1242/dev.126.10.2033. PMID   10207129.
• Wilkinson DG (2000). "Eph receptors and ephrins: regulators of guidance and assembly". Int. Rev. Cytol. International Review of Cytology. 196: 177–244. doi:10.1016/S0074-7696(00)96005-4. ISBN   9780123646002. PMID   10730216.
• Xu Q, Mellitzer G, Wilkinson DG (2000). "Roles of Eph receptors and ephrins in segmental patterning". Philos. Trans. R. Soc. Lond. B Biol. Sci. 355 (1399): 993–1002. doi:10.1098/rstb.2000.0635. PMC   1692797 . PMID   11128993.
• Wilkinson DG (2001). "Multiple roles of EPH receptors and ephrins in neural development". Nat. Rev. Neurosci. 2 (3): 155–64. doi:10.1038/35058515. PMID   11256076. S2CID   205014301.
• Kinch MS, Carles-Kinch K (2003). "Overexpression and functional alterations of the EphA2 tyrosine kinase in cancer". Clin. Exp. Metastasis. 20 (1): 59–68. doi:10.1023/A:1022546620495. PMID   12650608. S2CID   2472961.
• Walker-Daniels J, Hess AR, Hendrix MJ, Kinch MS (2003). "Differential regulation of EphA2 in normal and malignant cells". Am. J. Pathol. 162 (4): 1037–42. doi:10.1016/S0002-9440(10)63899-0. PMC   1851246 . PMID   12651595.
• Lindberg RA, Hunter T (1990). "cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases". Mol. Cell. Biol. 10 (12): 6316–24. doi:10.1128/mcb.10.12.6316. PMC   362907 . PMID   2174105.
• Pandey A, Shao H, Marks RM, Polverini PJ, Dixit VM (1995). "Role of B61, the ligand for the Eck receptor tyrosine kinase, in TNF-alpha-induced angiogenesis". Science. 268 (5210): 567–9. Bibcode:1995Sci...268..567P. doi:10.1126/science.7536959. PMID   7536959.
• Pandey A, Duan H, Dixit VM (1995). "Characterization of a novel Src-like adapter protein that associates with the Eck receptor tyrosine kinase". J. Biol. Chem. 270 (33): 19201–4. doi: 10.1074/jbc.270.33.19201 . PMID   7543898.
• Ruiz JC, Robertson EJ (1994). "The expression of the receptor-protein tyrosine kinase gene, eck, is highly restricted during early mouse development". Mech. Dev. 46 (2): 87–100. doi:10.1016/0925-4773(94)90078-7. PMID   7918100. S2CID   21419336.
• Davis S, Gale NW, Aldrich TH, Maisonpierre PC, Lhotak V, Pawson T, Goldfarb M, Yancopoulos GD (1994). "Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity". Science. 266 (5186): 816–9. Bibcode:1994Sci...266..816D. doi:10.1126/science.7973638. PMID   7973638.
• Pandey A, Lazar DF, Saltiel AR, Dixit VM (1994). "Activation of the Eck receptor protein tyrosine kinase stimulates phosphatidylinositol 3-kinase activity". J. Biol. Chem. 269 (48): 30154–7. doi: 10.1016/S0021-9258(18)43790-8 . PMID   7982920.
• Ganju P, Shigemoto K, Brennan J, Entwistle A, Reith AD (1994). "The Eck receptor tyrosine kinase is implicated in pattern formation during gastrulation, hindbrain segmentation and limb development". Oncogene. 9 (6): 1613–24. PMID   8183555.
• Gale NW, Holland SJ, Valenzuela DM, Flenniken A, Pan L, Ryan TE, Henkemeyer M, Strebhardt K, Hirai H, Wilkinson DG, Pawson T, Davis S, Yancopoulos GD (1996). "Eph receptors and ligands comprise two major specificity subclasses and are reciprocally compartmentalized during embryogenesis". Neuron. 17 (1): 9–19. doi: 10.1016/S0896-6273(00)80276-7 . PMID   8755474. S2CID   1075856.
• Kozlosky CJ, VandenBos T, Park L, Cerretti DP, Carpenter MK (1997). "LERK-7: a ligand of the Eph-related kinases is developmentally regulated in the brain". Cytokine. 9 (8): 540–9. doi:10.1006/cyto.1997.0199. PMID   9245480.
• Ephnomenclaturecommittee (1997). "Unified nomenclature for Eph family receptors and their ligands, the ephrins. Eph Nomenclature Committee". Cell. 90 (3): 403–4. doi: 10.1016/S0092-8674(00)80500-0 . PMID   9267020. S2CID   26773768.

External links

• Overview of all the structural information available in the PDB for UniProt : P29317 (Human Ephrin type-A receptor 2) at the PDBe-KB .
• Overview of all the structural information available in the PDB for UniProt : Q03145 (Mouse Ephrin type-A receptor 2) at the PDBe-KB .