N,N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase

Last updated
N,N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase
Identifiers
EC no. 2.4.1.290
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Search
PMC articles
PubMed articles
NCBI proteins

N,N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase (EC 2.4.1.290, PglA) is an enzyme with systematic name UDP-N-acetyl-alpha-D-galactosamine:N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol 3-alpha-N-acetyl-D-galactosaminyltransferase. [1] This enzyme catalyses the following chemical reaction

UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol

This enzyme is isolated from Campylobacter jejuni . It is important for N-linked glycosylation in this species. [2] The glycosyl motif that the enzymes encoded by the Pgl locus create consists of a bacillosamine joined to a GalNAc residue by an alpha 1–3 glycosidic bond, followed by four additional GalNAc residues linked by alpha 1–4 bonds. A branching glucose residue completes the heptasaccharide, joined by a beta 1–3 linkage to the third GalNAc residue from the amino acid linkage point. [3] Glycosylation takes place on an undecaprenyl pyrophosphate on the cytosolic face of the plasma membrane, followed by flippase transfer to the periplasmic space and en bloc transfer to an asparagine residue. [4]

This enzyme catalyzes the addition of GalNAc, covalently bonded to carrier uridine diphosphate (UDP), to isoprenoid lipid–linked bacillosamine, resulting in production of UDP and the glycosylated lipid. [1]

Related Research Articles

In enzymology, a globoside alpha-N-acetylgalactosaminyltransferase is an enzyme that catalyzes the chemical reaction

In enzymology, a globotriaosylceramide 3-beta-N-acetylgalactosaminyltransferase is an enzyme that catalyzes the chemical reaction

In enzymology, a polypeptide N-acetylgalactosaminyltransferase is an enzyme that catalyzes the chemical reaction

In enzymology, a glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase is an enzyme that catalyzes the chemical reaction

<span class="mw-page-title-main">GALNT3</span> Protein-coding gene in the species Homo sapiens

Polypeptide N-acetylgalactosaminyltransferase 3 is an enzyme that in humans is encoded by the GALNT3 gene.

<span class="mw-page-title-main">GALNT1</span> Protein-coding gene in the species Homo sapiens

Polypeptide N-acetylgalactosaminyltransferase 1 is an enzyme that in humans is encoded by the GALNT1 gene.

<span class="mw-page-title-main">GALNT2</span> Protein-coding gene in the species Homo sapiens

Polypeptide N-acetylgalactosaminyltransferase 2 is an enzyme that in humans is encoded by the GALNT2 gene.

<span class="mw-page-title-main">GALNT6</span> Protein-coding gene in humans

Polypeptide N-acetylgalactosaminyltransferase 6 is an enzyme that in humans is encoded by the GALNT6 gene.

<span class="mw-page-title-main">GALNT13</span> Protein-coding gene in the species Homo sapiens

Polypeptide N-acetylgalactosaminyltransferase 13 is an enzyme that in humans is encoded by the GALNT13 gene.

UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine N-acetyltransferase is an enzyme with systematic name acetyl-CoA:UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine N-acetyltransferase. This enzyme catalyses the following chemical reaction

(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase is an enzyme with systematic name UDP-N-acetyl-D-galactosamine:1-O-(O- - -O-beta-D-galactopyranosyl- -beta-D-glucopyranosyl)-ceramide 4-beta-N-acetyl-D-galactosaminyltransferase. This enzyme catalyses the following chemical reaction:

N-acetylneuraminylgalactosylglucosylceramide beta-1,4-N-acetylgalactosaminyltransferase (EC 2.4.1.165, is an enzyme that catalyses the following chemical reaction:

Glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase is an enzyme with systematic name UDP-N-acetyl-D-galactosamine:D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase. This enzyme catalyses the following chemical reaction

N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase is an enzyme with systematic name UDP-N-acetyl-D-galactosamine:N-acetyl-beta-D-glucosaminyl-group 4-beta-N-acetylgalactosaminyltransferase. This enzyme catalyses the following chemical reaction

N-acetylgalactosamine-N, N'-diacetylbacillosaminyl-diphospho-undecaprenol 4-alpha-N-acetylgalactosaminyltransferase is an enzyme with systematic name UDP-N-acetyl-alpha-D-galactosamine:N-acetylgalactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol 3-alpha-N-acetyl-D-galactosaminyltransferase. This enzyme catalyses the following chemical reaction

GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase is an enzyme with systematic name UDP-N-acetyl-alpha-D-galactosamine:GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-tritrans,heptacis-undecaprenol 4-alpha-N-acetyl-D-galactosaminyltransferase. This enzyme catalyses the following chemical reaction

GalNAc5-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase is an enzyme with systematic name UDP-alpha-D-glucose:(GalNAc-alpha- )4-GalNAc-alpha-(1->3)-diNAcBac-diphospho-tritrans,heptacis-undecaprenol 3-beta-D-glucosyltransferase. This enzyme catalyses the following chemical reaction

Undecaprenyl-diphosphooligosaccharide-protein glycotransferase is an enzyme with systematic name tritrans,heptacis-undecaprenyl-diphosphooligosaccharide:protein-L-asparagine N-beta-D-oligosaccharidotransferase. This enzyme catalyses the following chemical reaction

UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine transaminase is an enzyme with systematic name UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine:2-oxoglutarate aminotransferase. This enzyme catalyses the following chemical reaction

Phosphoglycosyl transferase C (PglC) is an enzyme belonging to a class known as monotopic phosphoglycosyl transferases (PGT). PGTs are required for the synthesis of glycoconjugates on the membrane surface of bacteria. Glycoconjugates, such as glycoproteins, are imperative for bacterial communication as well as host cell interactions between prokaryotic and eukaryotic cells lending to bacteria's pathogenicity.

References

  1. 1 2 Glover KJ, Weerapana E, Imperiali B (October 2005). "In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation". Proceedings of the National Academy of Sciences of the United States of America. 102 (40): 14255–9. Bibcode:2005PNAS..10214255G. doi: 10.1073/pnas.0507311102 . PMC   1242339 . PMID   16186480.
  2. Karlyshev AV, Ketley JM, Wren BW (2005). "The Campylobacter jejuni Glycome". FEMS Microbiology Reviews. 29 (2): 377–390. doi: 10.1016/j.fmrre.2005.01.003 . PMID   15808749.
  3. Young NM, Brisson JR, Kelly J, Watson DC, Tessier L, Lanthier PH, Jarrell HC, Cadotte N, St Michael F, Aberg E, Szymanski CM (2002). "Structure of the N-linked glycan present on multiple glycoproteins in the Gram-negative bacterium, Campylobacter jejuni". Journal of Biological Chemistry. 277 (45): 42530–42539. doi: 10.1074/jbc.M206114200 . PMID   12186869.
  4. Alaimo C, Catrein I, Morf L, Marolda CL, Callewaert N, Valvano MA, Feldman MF, Aebi M (2006). "Two distinct but interchangeable mechanisms for flipping of lipid-linked oligosaccharides". The EMBO Journal. 25 (5): 967–976. doi: 10.1038/sj.emboj.7601024 . PMC   1409731 . PMID   16498400.