Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase

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Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
Identifiers
EC no. 2.4.2.43
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Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase (EC 2.4.2.43, undecaprenyl phosphate-alpha-L-Ara4N transferase, 4-amino-4-deoxy-L-arabinose lipid A transferase, polymyxin resistance protein PmrK, arnT (gene)) is an enzyme with systematic name 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans, octacis-undecaprenyl phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase. [1] [2] [3] [4] [5] This enzyme catalyses the following chemical reaction

(1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans, octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans, octacis-undecaprenyl phosphate
(2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans, octacis-undecaprenyl phosphate + lipid IVA lipid IIA + ditrans, octacis-undecaprenyl phosphate
(3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans, octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans, octacis-undecaprenyl phosphate

This integral membrane protein is present in the inner membrane of certain Gram negative endobacteria.

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In molecular biology, the lipopolysaccharide kinase (Kdo/WaaP) family is a family of lipopolysaccharide kinases that includes lipopolysaccharide core heptose(I) kinase rfaP. Lipopolysaccharide core heptose(I) kinase rfaP is required for the addition of phosphate to O-4 of the first heptose residue of the lipopolysaccharide (LPS) inner core region. It has previously been shown that it is necessary for resistance to hydrophobic and polycationic antimicrobials in E. coli and that it is required for virulence in invasive strains of Salmonella enterica. The family also includes 3-deoxy-D-manno-octulosonic acid kinase from Haemophilus influenzae, which phosphorylates Kdo-lipid IV(A), a lipopolysaccharide precursor, and is involved in virulence.

UDP-glucuronic acid dehydrogenase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305, UDP-GlcUA decarboxylase, ArnADH) is an enzyme with systematic name UDP-glucuronate:NAD+ oxidoreductase (decarboxylating). This enzyme catalyses the following chemical reaction

UDP-4-amino-4-deoxy-L-arabinose formyltransferase is an enzyme with systematic name 10-formyltetrahydrofolate:UDP-4-amino-4-deoxy-beta-L-arabinose N-formyltransferase. This enzyme catalyses the following chemical reaction

Lipid II:glycine glycyltransferase (EC 2.3.2.16, N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:N6-glycine transferase, femX (gene)) is an enzyme with systematic name alanyl-D-alanine-diphospho-ditrans, octacis-undecaprenyl-N-acetylglucosamine:glycine N6-glycyltransferase. This enzyme catalyses the following chemical reaction

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase (EC 2.3.2.18, femB (gene)) is an enzyme with systematic name N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase. This enzyme catalyses the following chemical reaction

GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase is an enzyme with systematic name GDP-mannose:D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase . This enzyme catalyses the following chemical reaction

Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase. This enzyme catalyses the following chemical reaction

(KDO)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:(KDO)-lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase. This enzyme catalyses the following chemical reaction

(KDO)2-lipid IVA (2-8) 3-deoxy-D-manno-octulosonic acid transferase is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:(KDO)2-lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase . This enzyme catalyses the following chemical reaction

(KDO)3-lipid IVA (2-4) 3-deoxy-D-manno-octulosonic acid transferase is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:(KDO)3-lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase . This enzyme catalyses the following chemical reaction

Xanthan ketal pyruvate transferase is an enzyme with systematic name phosphoenolpyruvate:D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 4,6-O-(1-carboxyethan-1,1-diyl)transferase. This enzyme catalyses the following chemical reaction

UDP-4-amino-4-deoxy-L-arabinose aminotransferase is an enzyme with systematic name UDP-4-amino-4-deoxy-beta-L-arabinose:2-oxoglutarate aminotransferase. This enzyme catalyses the following chemical reaction

3-deoxy-D-manno-octulosonic acid kinase is an enzyme with systematic name ATP:(KDO)-lipid IVA 3-deoxy-alpha-D-manno-oct-2-ulopyranose 4-phosphotransferase. This enzyme catalyses the following chemical reaction

Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase is an enzyme with systematic name UDP-4-amino-4-deoxy-alpha-L-arabinose:ditrans,octacis-undecaprenyl phosphate 4-amino-4-deoxy-alpha-L-arabinosyltransferase. This enzyme catalyses the following chemical reaction

Undecaprenyl-phosphate glucose phosphotransferase is an enzyme with systematic name UDP-glucose:ditrans,octacis-undecaprenyl-phosphate glucose phosphotransferase. This enzyme catalyses the following chemical reaction

UDP-N-acetylglucosamine—undecaprenyl-phosphate N-acetylglucosaminephosphotransferase is an enzyme with systematic name UDP-N-acetyl-alpha-D-glucosamine:ditrans,octacis-undecaprenyl phosphate N-acetyl-alpha-D-glucosaminephosphotransferase. This enzyme catalyses the following chemical reaction

UDP-N-acetylglucosamine---decaprenyl-phosphate N-acetylglucosaminephosphotransferase is an enzyme with systematic name UDP-N-acetyl-alpha-D-glucosamine:trans,octacis-decaprenyl-phosphate N-acetylglucosaminephosphotransferase. This enzyme catalyses the following chemical reaction

References

  1. Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR (November 2001). "An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor". The Journal of Biological Chemistry. 276 (46): 43122–31. doi: 10.1074/jbc.m106961200 . PMID   11535604.
  2. Trent MS, Ribeiro AA, Doerrler WT, Lin S, Cotter RJ, Raetz CR (November 2001). "Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm". The Journal of Biological Chemistry. 276 (46): 43132–44. doi: 10.1074/jbc.m106962200 . PMID   11535605.
  3. Zhou Z, Ribeiro AA, Lin S, Cotter RJ, Miller SI, Raetz CR (November 2001). "Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation". The Journal of Biological Chemistry. 276 (46): 43111–21. doi: 10.1074/jbc.m106960200 . PMID   11535603.
  4. Bretscher LE, Morrell MT, Funk AL, Klug CS (March 2006). "Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium". Protein Expression and Purification. 46 (1): 33–9. doi:10.1016/j.pep.2005.08.028. PMID   16226890.
  5. Impellitteri NA, Merten JA, Bretscher LE, Klug CS (January 2010). "Identification of a functionally important loop in Salmonella typhimurium ArnT". Biochemistry. 49 (1): 29–35. doi:10.1021/bi901572f. PMC   2805049 . PMID   19947657.