Procollagen galactosyltransferase

Last updated
procollagen galactosyltransferase
Identifiers
EC no. 2.4.1.50
CAS no. 9028-07-3
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / QuickGO
Search
PMC articles
PubMed articles
NCBI proteins

In enzymology, a procollagen galactosyltransferase (EC 2.4.1.50) is an enzyme that catalyzes the chemical reaction

UDP-galactose + procollagen 5-hydroxy-L-lysine UDP + procollagen 5-(D-galactosyloxy)-L-lysine

Thus, the two substrates of this enzyme are UDP-galactose and procollagen 5-hydroxy-L-lysine, whereas its two products are UDP and procollagen 5-(D-galactosyloxy)-L-lysine.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-galactose:procollagen-5-hydroxy-L-lysine D-galactosyltransferase. Other names in common use include hydroxylysine galactosyltransferase, collagen galactosyltransferase, collagen hydroxylysyl galactosyltransferase, UDP galactose-collagen galactosyltransferase, uridine diphosphogalactose-collagen galactosyltransferase, and UDPgalactose:5-hydroxylysine-collagen galactosyltransferase. This enzyme participates in lysine degradation.

Structure and functons of procollagen galactosyltransferase 1

Procollagen galactosyltransferase 1 (GT251), encoded by the COLGALT1 gene, plays a crucial role in lysyl O-linked glycosylation and the maturation of collagen. [1] GT251 consists of two galactosyltransferase domains (GalT-N and GalT-C) and is stabilized in a dimeric form. [2] The GT251 dimer can further associate with LH3 (encoded by PLOD3) to form a heterotetrameric complex, known as the KOGG complex (Lysyl Hydroxylation-Galactosylation-Glucosylation complex). [2] Within this complex, three key enzymatic reactions in lysine O-linked glycosylation are coordinately catalyzed by LH3 and GT251, ensuring proper collagen modification and structural integrity.

References

  1. Schegg, Belinda; Hülsmeier, Andreas J.; Rutschmann, Christoph; Maag, Charlotte; Hennet, Thierry (2009-02-01). "Core Glycosylation of Collagen Is Initiated by Two β(1- O )Galactosyltransferases". Molecular and Cellular Biology. 29 (4): 943–952. doi:10.1128/MCB.02085-07. ISSN   1098-5549. PMC   2643808 . PMID   19075007.
  2. 1 2 Peng, Junjiang; Li, Wenguo; Yao, Deqiang; Xia, Ying; Wang, Qian; Cai, Yan; Li, Shaobai; Cao, Mi; Shen, Yafeng; Ma, Peixiang; Liao, Rijing; Zhao, Jie; Qin, An; Cao, Yu (2025-03-11). "The structural basis for the human procollagen lysine hydroxylation and dual-glycosylation". Nature Communications. 16 (1). doi:10.1038/s41467-025-57768-9. ISSN   2041-1723.