| District | Senator | Party | Constituency | Began serving | 
|---|
 |  1st  |     |  Alanson T. Lincoln  |  Republican  |  Washington, Smyth, and city of Bristol  | 1908 | 
 |  2nd  |     |  John C. Noel  |  Republican  |  Scott, Lee, and Wise  | 1904 | 
 |  3rd  |     |  Roland E. Chase  |  Republican  |  Buchanan, Dickenson, Russell, and Tazewell  | 1908 | 
 |  4th  |     |  John M. Hart  |  Democratic  |  Roanoke, Montgomery, and cities of Roanoke and Radford  | 1908 | 
 |  5th  |     |  A. Pendleton Strother  |  Republican  |  Giles, Bland, Pulaski, and Wythe  | 1908 | 
 |  6th  |     |  John M. Parsons  |  Republican  |  Carroll, Grayson, and Patrick  | 1908 | 
 |  7th  |     |  Floyd W. King  |  Democratic  |  Craig, Botetourt, Allegheny, Bath, and city of Clifton Forge  | 1908 | 
 |  8th  |     |  George B. Keezell  |  Democratic  |  Rockingham  | 1896 (previously served 1884–1887) | 
 |  9th  |     |  Edward Echols  |  Democratic  |  Augusta, Highland, and city of Staunton  | 1906 (previously served 1889–1897) | 
 |  10th  |     |  Robert M. Ward  |  Democratic  |  Shenandoah, Frederick, and city of Winchester  | 1908 | 
 |  11th  |     |  G. Latham Fletcher  |  Democratic  |  Fauquier and Loudoun  | 1908 | 
 |  12th  |     |  Richard S. Parks  |  Democratic  |  Clarke, Page, and Warren  | 1908 | 
 |  13th  |     |  F. Wilmer Sims  |  Democratic  |  Spotsylvania, Stafford, Louisa, and city of Fredericksburg  | 1906 | 
 |  14th  |     |  R. Ewell Thornton  |  Democratic  |  Alexandria county, Prince William, Fairfax, and city of Alexandria  | 1908 | 
 |  15th  |     |  F. Pendleton Carter  |  Democratic  |  Culpeper, Madison, Rappahannock, and Orange  | 1908 | 
 |  16th  |     |  John B. Watkins  |  Democratic  |  Goochland, Powhatan, and Chesterfield  | 1908 | 
 |  17th  |     |  Nathaniel B. Early  |  Democratic  |  Albemarle, Greene, and city of Charlottesville  | 1908 | 
 |  18th  |     |  William C. White  |  Democratic  |  Appomattox, Buckingham, Fluvanna, and Charlotte  | 1908 | 
 |  19th  |     |  Aubrey E. Strode  |  Democratic  |  Amherst and Nelson  | 1906 | 
 |  20th  |     |  Don P. Halsey  |  Democratic  |  Campbell and city of Lynchburg  | 1908 (previously served 1902–1904) | 
 |  21st  |     |  Henry A. Edmondson  |  Democratic  |  Halifax  | 1908 | 
 |  22nd  |     |  J. Randolph Tucker  |  Democratic  |  Bedford, Rockbridge, and city of Buena Vista  | 1908 | 
 |  23rd  |     |  William A. Garrett  |  Democratic  |  Pittsylvania, Henry, and city of Danville  | 1901 | 
 |  24th  |     |  George T. Rison  |  Democratic  |  Pittsylvania and city of Danville  | 1904 | 
 |  25th  |     |  James D. Elam  |  Democratic  |  Mecklenburg and Brunswick  | 1908 (previously served 1901–1904) | 
 |  26th  |     |  Greenville O. McAlexander  |  Republican  |  Franklin and Floyd  | 1908 | 
 |  27th  |     |  Alexander R. Hobbs  |  Democratic  |  Greensville, Sussex, Surry, and Prince George  | 1901 | 
 |  28th  |     |  William Hodges Mann  |  Democratic  |  Nottoway, Amelia, Lunenburg, Prince Edward, and Cumberland  | 1899 | 
 |  29th  |     |  Charles T. Lassiter  |  Democratic  |  Dinwiddie and city of Petersburg  | 1906 | 
 |  30th  |     |  Edward E. Holland  |  Democratic  |  Isle of Wight, Southampton, and Nansemond  | 1908 | 
 |  31st  |     |  William W. Sale  |  Democratic  |  Norfolk city | 1901 | 
 |  32nd  |     |  Charles U. Gravatt  |  Democratic  |  Caroline, Hanover, and King William  | 1908 | 
 |  33rd  |     |  John A. Lesner  |  Democratic  |  Norfolk county and city of Portsmouth  | 1908 | 
 |  34th  |     |  C. Harding Walker  |  Democratic  |  King George, Richmond, Westmoreland, Lancaster, and Northumberland  | 1899 | 
 |  35th  |     |  T. Ashby Wickham  |  Democratic  |  Henrico, New Kent, Charles City, James City, and city of Williamsburg  | 1906 | 
 |  36th  |     |  Saxon W. Holt  |  Democratic  |  Elizabeth City, York, Warwick, and city of Newport News  | 1904 | 
 |  37th  |     |  Ben T. Gunter  |  Democratic  |  Accomac, Northampton, and Princess Anne  | 1904 | 
 |  38th  |     |  Elben C. Folkes  |  Democratic  |  Richmond city | 1908 | 
 |     |  Arthur C. Harman  |  Democratic  | 1904 | 
 |  39th  |     |  John R. Saunders  |  Democratic  |  King and Queen, Middlesex, Essex, Gloucester, and Mathews  | 1908 |