Laboratory water bath

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Inside a shaking water bath Shaking water bath 2015.JPG
Inside a shaking water bath
A water bath operating at 72degC Circulating water bath 2015.jpg
A water bath operating at 72°C

A water bath is laboratory equipment made from a container filled with heated water. It is used to incubate samples in water at a constant temperature over a long period of time. Most water baths have a digital or an analogue interface to allow users to set a desired temperature, but some water baths have their temperature controlled by a current passing through a reader.

Contents

Uses include warming of reagents, melting of substrates, determination of boiling point, or incubation of cell cultures. It is also used to enable certain chemical reactions to occur at high temperature.

Water baths are preferred heat sources for heating flammable chemicals, as their lack of open flame prevents ignition. [1] Different types of water baths are used depending on application. For all water baths, it can be used up to 99.9 °C. [2] [3]

When the required temperature is above 100 °C, alternative methods such as oil bath, silicone oil bath or sand bath may be used. [4]

Precautions

Types of water bath

A shaking water bath in action

Circulating water baths

Circulating water baths (also called stirrers [10] ) are ideal for applications when temperature uniformity and consistency are critical, such as enzymatic and serologic experiments. Water is thoroughly circulated throughout the bath resulting in a more uniform temperature.

Non-circulating water baths

This type of water bath relies primarily on convection instead of water being uniformly heated. Therefore, it is less accurate in terms of temperature control. In addition, there are add-ons that provide stirring to non-circulating water baths to create more uniform heat transfer. [4]

Shaking water baths

This type of water bath has extra control for shaking, which moves liquids around. This shaking feature can be turned on or off. In microbiological practices, constant shaking allows liquid-grown cell cultures grown to constantly mix with the air.

Some key benefits of shaking water bath are user-friendly operation via keypad, convenient bath drains, adjustable shaking frequencies, bright LED-display, optional lift-up bath cover, power switch integrated in keypad and warning and cut-off protection for low/high temperature.

Water bath technologies

The bath is a fundamental product in any laboratory. Over the years, water baths have evolved from basic analog tools to advanced digital machines capable of sophisticated and programmable controls, functions, and capabilities.

Key features in water baths often include:

Additional images

See also

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References

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  4. 1 2 "Baths and Circulators" (PDF). pubs.acs.org. October 2004.
  5. 1 2 "Standard Operating Procedure: Heat Sources and Heating Baths" (PDF). naples.cc.sunysb.edu. February 2013. Archived from the original (PDF) on 2018-05-16.
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  7. 1 2 "Standard Operating Procedures Manual: Biosafety Level 2 Laboratory, Arizona State University".
  8. 1 2 Cheesbrough, Monica (2005-09-08). District Laboratory Practice in Tropical Countries. Cambridge University Press. ISBN   9780521676304.
  9. 1 2 "Fisher Isotemp Water Baths" (PDF). www.seas.upenn.edu. Retrieved 2015-07-09.
  10. "Water Baths, Stir and Hot Plates" (PDF). library.ewh.org.
  11. "Beads for Baths".