A water bath is laboratory equipment made from a container filled with heated water. It is used to incubate samples in water at a constant temperature over a long period of time. Most water baths have a digital or an analogue interface to allow users to set a desired temperature, but some water baths have their temperature controlled by a current passing through a reader.
Uses include warming of reagents, melting of substrates, determination of boiling point, or incubation of cell cultures. It is also used to enable certain chemical reactions to occur at high temperature.
Water baths are preferred heat sources for heating flammable chemicals, as their lack of open flame prevents ignition. [1] Different types of water baths are used depending on application. For all water baths, it can be used up to 99.9 °C. [2] [3]
When the required temperature is above 100 °C, alternative methods such as oil bath, silicone oil bath or sand bath may be used. [4]
This section is written like a manual or guide.(February 2020) |
Circulating water baths (also called stirrers [10] ) are ideal for applications when temperature uniformity and consistency are critical, such as enzymatic and serologic experiments. Water is thoroughly circulated throughout the bath resulting in a more uniform temperature.
This type of water bath relies primarily on convection instead of water being uniformly heated. Therefore, it is less accurate in terms of temperature control. In addition, there are add-ons that provide stirring to non-circulating water baths to create more uniform heat transfer. [4]
This type of water bath has extra control for shaking, which moves liquids around. This shaking feature can be turned on or off. In microbiological practices, constant shaking allows liquid-grown cell cultures grown to constantly mix with the air.
Some key benefits of shaking water bath are user-friendly operation via keypad, convenient bath drains, adjustable shaking frequencies, bright LED-display, optional lift-up bath cover, power switch integrated in keypad and warning and cut-off protection for low/high temperature.
The bath is a fundamental product in any laboratory. Over the years, water baths have evolved from basic analog tools to advanced digital machines capable of sophisticated and programmable controls, functions, and capabilities.
Key features in water baths often include:
A bain-marie, also known as a water bath or double boiler, a type of heated bath, is a piece of equipment used in science, industry, and cooking to heat materials gently or to keep materials warm over a period of time. A bain-marie is also used to melt ingredients for cooking.
Paraffin wax is a soft colorless solid derived from petroleum, coal, or oil shale that consists of a mixture of hydrocarbon molecules containing between 20 and 40 carbon atoms. It is solid at room temperature and begins to melt above approximately 37 °C (99 °F), and its boiling point is above 370 °C (698 °F). Common applications for paraffin wax include lubrication, electrical insulation, and candles; dyed paraffin wax can be made into crayons. It is not to be confused with kerosene and other petroleum products that are sometimes called paraffin.
A rotary evaporator (rotavap) is a device used in chemical laboratories for the efficient and gentle removal of solvents from samples by evaporation. When referenced in the chemistry research literature, description of the use of this technique and equipment may include the phrase "rotary evaporator", though use is often rather signaled by other language.
A deep fryer is a kitchen appliance used for deep frying. Deep frying is a method of cooking by submerging food into oil at high heat, typically between temperatures of 350 to 375 °F.
A hot plate or hotplate is a portable self-contained tabletop small appliance cooktop that features one or more electric heating elements or gas burners. A hot plate can be used as a stand-alone appliance, but is often used as a substitute for one of the burners from an oven range or a kitchen stove. Hot plates are often used for food preparation, generally in locations where a full kitchen stove would not be convenient or practical. A hot plate can have a flat surface or round surface. Hot plates can be used for traveling or in areas without electricity.
Cell disruption is a method or process for releasing biological molecules from inside a cell.
Hydronics is the use of liquid water or gaseous water (steam) or a water solution as a heat-transfer medium in heating and cooling systems. The name differentiates such systems from oil and refrigerant systems.
Transformer oil or insulating oil is an oil that is stable at high temperatures and has excellent electrical insulating properties. It is used in oil-filled wet transformers, some types of high-voltage capacitors, fluorescent lamp ballasts, and some types of high-voltage switches and circuit breakers. Its functions are to insulate, suppress corona discharge and arcing, and to serve as a coolant.
A circulator pump or circulating pump is a specific type of pump used to circulate gases, liquids, or slurries in a closed circuit. They are commonly found circulating water in a hydronic heating or cooling system. Because they only circulate liquid within a closed circuit, they only need to overcome the friction of a piping system.
A sand bath is a common piece of laboratory equipment made from a container filled with heated sand. It is used to evenly heat another container, most often during a chemical reaction.
Vapour-compression refrigeration or vapor-compression refrigeration system (VCRS), in which the refrigerant undergoes phase changes, is one of the many refrigeration cycles and is the most widely used method for air conditioning of buildings and automobiles. It is also used in domestic and commercial refrigerators, large-scale warehouses for chilled or frozen storage of foods and meats, refrigerated trucks and railroad cars, and a host of other commercial and industrial services. Oil refineries, petrochemical and chemical processing plants, and natural gas processing plants are among the many types of industrial plants that often utilize large vapor-compression refrigeration systems. Cascade refrigeration systems may also be implemented using two compressors.
A heated bath is used in the laboratory to allow a chemical reaction to occur at an elevated temperature.
A heating mantle, or isomantle, is a piece of laboratory equipment used to apply heat to containers, as an alternative to other forms of heated bath. In contrast to other heating devices, such as hotplates or Bunsen burners, glassware containers may be placed in direct contact with the heating mantle without substantially increasing the risk of the glassware shattering, because the heating element of a heating mantle is insulated from the container so as to prevent excessive temperature gradients. Heating mantles may have various forms. In a common arrangement, electric wires are embedded within a strip of fabric that can be wrapped around a flask. The current supplied to the device, and hence the temperature achieved, is regulated by a rheostat. This type of heating mantle is quite useful for maintaining an intended temperature within a separatory funnel, for example, after the contents of a reaction have been removed from a primary heat source.
A cooling bath or ice bath, in laboratory chemistry practice, is a liquid mixture which is used to maintain low temperatures, typically between 13 °C and −196 °C. These low temperatures are used to collect liquids after distillation, to remove solvents using a rotary evaporator, or to perform a chemical reaction below room temperature.
A thermostatic mixing valve (TMV) is a valve that blends hot water with cold water to ensure constant, safe shower and bath outlet temperatures to prevent scalding.
An oil bath is a type of heated bath used in a laboratory, most commonly used to heat up chemical reactions. It is a container of oil that is heated by a hot plate or a Bunsen burner.
The Thiele tube, named after the German chemist Johannes Thiele, is a laboratory glassware designed to contain and heat an oil bath. Such a setup is commonly used in the determination of the melting point or boiling point of a substance. The apparatus resembles a glass test tube with an attached handle.
Reflux is a technique involving the condensation of vapors and the return of this condensate to the system from which it originated. It is used in industrial and laboratory distillations. It is also used in chemistry to supply energy to reactions over a long period of time.
HVAC is a major sub discipline of mechanical engineering. The goal of HVAC design is to balance indoor environmental comfort with other factors such as installation cost, ease of maintenance, and energy efficiency. The discipline of HVAC includes a large number of specialized terms and acronyms, many of which are summarized in this glossary.
Immersion cooling is an IT cooling practice by which complete servers are immersed in a dielectric, electrically non-conductive fluid that has significantly higher thermal conductivity than air. Heat is removed from a system by putting the coolant in direct contact with hot components, and circulating the heated liquid through heat exchangers. This practice is highly effective because liquid coolants can absorb more heat from the system, and are more easily circulated through the system, than air. Immersion cooling has many benefits, including but not limited to: sustainability, performance, reliability and cost
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