Laboratory glassware

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Three beakers, an Erlenmeyer flask, a graduated cylinder and a volumetric flask Lab glassware.jpg
Three beakers, an Erlenmeyer flask, a graduated cylinder and a volumetric flask

Laboratory glassware is a variety of equipment used in scientific work, traditionally made of glass. Glass may be blown, bent, cut, molded, or formed into many sizes and shapes. It is commonly used in chemistry, biology, and analytical laboratories. Many laboratories have training programs to demonstrate how glassware is used and to alert first–time users to the safety hazards involved with using glassware.

Contents

History

Late 17th-century laboratory glassware in the painting by Cornelis de Man (National Museum in Warsaw). Man Group portrait (detail).jpg
Late 17th-century laboratory glassware in the painting by Cornelis de Man (National Museum in Warsaw).

Ancient era

The history of glassware dates back to the Phoenicians who fused obsidian together in campfires making the first glassware. Glassware evolved as other ancient civilizations including the Syrians, Egyptians, and Romans refined the art of glassmaking. Mary the Jewess, an alchemist in Alexandria during the 1st century AD, is credited for the creation of some of the first glassware for chemical such as the kerotakis which was used for the collection of fumes from a heated material. [1] Despite these creations, glassware for chemical uses was still limited during this time because of the low thermal stability necessary for experimentation and therefore was primarily made using copper or ceramic materials. [1]

Early modern era

Glassware improved once again during the 14th-16th century, with the skill and knowledge of glass makers in Venice. During this time, the Venetians gathered knowledge about glassmaking from the East with information coming from Syria and the Byzantine Empire. [1] Along with knowledge about glassmaking, glassmakers in Venice also received higher quality raw materials from the East such as imported plant ash which contained higher soda content compared to plant ash from other areas. [1] This combination of better raw materials and information from the East led to the production of clearer and higher thermal and chemical durability leading towards the shift to the use of glassware in laboratories. [1]

Modern era

Many glasses that were produced in bulk in the 1830s would quickly become unclear and dirty because of the low quality glass being used. [2] During the 19th century, more chemists began to recognize the importance of glassware due to its transparency, and the ability to control the conditions of experiments. [3] Jöns Jacob Berzelius, who invented the test tube, and Michael Faraday both contributed to the rise of chemical glassblowing. Faraday published Chemical Manipulation in 1827 which detailed the process for creating many types of small tube glassware and some experimental techniques for tube chemistry. [3] [4] Berzelius wrote a similar textbook titled Chemical Operations and Apparatus which provided a variety of chemical glassblowing techniques. [3] The rise of this chemical glassblowing widened the availability of chemical experimentation and led to a shift towards the dominant use of glassware in laboratories. With the emergence of glassware in laboratories, the need for organization and standards arose. The Prussian Society for the Advancement of Industry was one of the earliest organizations to support the collaborative improvement of the quality of glass used. [5]

Following the development of borosilicate glass by Otto Schott in the late 19th century, most laboratory glassware was manufactured in Germany up until the start of World War I. [6] Before World War I, glass producers in the United States had difficulty competing with German laboratory glassware manufacturers because laboratory glassware was classified as educational material and was not subject to an import tax. During World War I, the supply of laboratory glassware to the United States was cut off. [6]

In 1915 Corning Glassworks developed their own borosilicate glass, introduced under the name Pyrex. This was a boon to the war effort in the United States. [6] Though many laboratories turned back to imports after the war ended, research into better glassware flourished. Glassware became more resistant to thermal shock while maintaining chemical inertness. [7]

During the 1920s efforts to standardise the dimensions of laboratory glassware began, particularly for ground glass joints, with some manufacturer specific standardisation beginning to occur around this time. Commercial standards began development around 1930, allowing the compatibility of joints between different manufacturers for the first time, along with other features. [8] [9] This quickly led to the high degree of standardisation and modularity seen in modern glassware.

Laboratory glassware selection

Laboratory glassware is typically selected by a person in charge of a particular laboratory analysis to match the needs of a given task. The task may require a piece of glassware made with a specific type of glass. The task may be readily performed using low cost, mass-produced glassware, or it may require a specialized piece created by a glass blower. The task may require controlling the flow of fluid. The task may have distinctive quality assurance requirements.

Type of glass

Brown glass jars with some clear lab glassware in the background Verrerie-p1030903.jpg
Brown glass jars with some clear lab glassware in the background

Laboratory glassware may be made from several types of glass, each with different capabilities and used for different purposes. Borosilicate glass is a type of transparent glass that is composed of boron oxide and silica, its main feature is a low coefficient of thermal expansion making it more resistant to thermal shock than most other glasses. [10] Quartz glass can withstand very high temperatures and is transparent in certain parts of the electromagnetic spectrum. Darkened brown or amber (actinic) glass can block ultraviolet and infrared radiation. Heavy-wall glass can withstand pressurized applications. Fritted glass is finely porous glass through which gas or liquid may pass. Coated glassware is specially treated to reduce the occurrence of breakage or failure. Silanized (siliconized) glassware is specially treated to prevent organic samples from sticking to the glass. [11]

Scientific glass blowing

Scientific glass blowing, which is practiced in some larger laboratories, is a specialized field of glassblowing. Scientific glassblowing involves precisely controlling the shape and dimension of glass, repairing expensive or difficult-to-replace glassware, and fusing together various glass parts. Many parts are available fused to a length of glass tubing to create highly specialized piece of laboratory glassware.

Controlling fluid flow

When using glassware it is often necessary to control the flow of fluid. It is commonly stopped with a stopper. Fluid may be transported between connected pieces of glassware. Types of interconnecting components include glass tubing, T-connectors, Y-connectors, and glass adapters. For a leak-tight connection a ground glass joint is used (possibly reinforced using a clamping method such as a Keck clips). Another way to connect glassware is with a hose barb and flexible tubing. Fluid flow can be switched selectively using a valve, of which a stopcock is a common type fused to the glassware. Valves made entirely of glass may be used to restrict fluid flows. Fluid, or any material which flows, can be directed into a narrow opening using a funnel.

Quality assurance

Metrology

Laboratory glassware can be used for high precision volumetric measurements. With high precision measurements, such as those made in a testing laboratory, the metrological grade of the glassware becomes important. The metrological grade then can be determined by both the confidence interval around the nominal value of measurement marks and the traceability of the calibration to an NIST standard. Periodically it may be necessary to check the calibration of the laboratory glassware. [12]

Dissolved silica

Laboratory glassware is composed of silica, which is considered insoluble in most substances, with a few exceptions such as hydrofluoric acid or strong alkali hydroxides. Though insoluble, a minute quantity of silica will dissolve in neutral water, which may affect high precision, low threshold measurements of silica in water. [13]

Cleaning

Cleaning laboratory glassware in a dishwasher Caricamento della vetreria di laboratorio in lavastoviglie.jpg
Cleaning laboratory glassware in a dishwasher

Cleaning laboratory glassware is a frequent necessity and may be done using multiple methods depending on the nature of the contamination and the purity requirements of its use. Glassware can be soaked in a detergent solution to remove grease and loosen most contaminations, these contaminations are then scrubbed with a brush or scouring pad to remove particles which cannot be rinsed. Sturdy glassware may be able to withstand sonication as an alternative to scrubbing. Solvents are used to remove organic residues that soap cannot remove, and inorganic residues that do not dissolve in water can often be dissolved with a dilute acid. When cleaning is finished it is common practice to rinse glassware multiple times, often finally with deionised water, before suspending it upside down on drying racks. [14] Specialised dishwashers can be used to automate these cleaning methods.

Resistant residues may require more powerful cleaning methods. Base baths are commonly used for organic residues, although the strong alkaline conditions do slowly dissolve the glass itself, and concentrated hydrochloric acid is common for removing inorganic residues. [15] Even more severe methods exist, such as acidic peroxide (piranha solution), aqua regia, and chromic acid, but these are considered somewhat of a last resort due to the hazards of using them, and their use by students is restricted in many institutions. [14]

For certain sensitive experiments glassware may require specialised procedures and ultra-pure water or solvents to dissolve trace quantities of specific contaminations known to interfere with an experiment. [16]

Examples

There are many different kinds of laboratory glassware items:

Examples of glassware containers include:

Examples of glassware used for measurements include:

Other examples of glassware includes:

Related Research Articles

<span class="mw-page-title-main">Glass</span> Transparent non-crystalline solid material

Glass is an amorphous (non-crystalline) solid. Because it is often transparent and chemically inert, glass has found widespread practical, technological, and decorative use in window panes, tableware, and optics. Some common objects made of glass like "a glass" of water, "glasses", and "magnifying glass", are named after the material.

<span class="mw-page-title-main">Florence flask</span> Glass laboratory container

A Florence flask/boiling flask is a type of flask used as an item of laboratory glassware and is named after the city Florence. It is used as a container to hold liquids. A Florence flask has a round body, a long neck, and often a flat bottom. It is designed for uniform heating, boiling, distillation and ease of swirling; it is produced in a number of different glass thicknesses to stand different types of use. They are often made of borosilicate glass for heat and chemical resistance. Traditional Florence flasks typically do not have a ground glass joint on their rather longer necks, but typically have a slight lip or flange around the tip of the neck. The common volume for a Florence flask is 1 litre.

A burette is a graduated glass tube with a tap at one end, for delivering known volumes of a liquid, especially in titrations. It is a long, graduated glass tube, with a stopcock at its lower end and a tapered capillary tube at the stopcock's outlet. The flow of liquid from the tube to the burette tip is controlled by the stopcock valve.

<span class="mw-page-title-main">Gas syringe</span> Tool used in a laboratory

A gas syringe is a piece of laboratory glassware used to insert or withdraw a volume of a gas from a closed system, or to measure the volume of gas evolved from a chemical reaction. A gas syringe can also be used to measure and dispense liquids, especially where these liquids need to be kept free from air.

<span class="mw-page-title-main">Borosilicate glass</span> Glass made of silica and boron trioxide

Borosilicate glass is a type of glass with silica and boron trioxide as the main glass-forming constituents. Borosilicate glasses are known for having very low coefficients of thermal expansion, making them more resistant to thermal shock than any other common glass. Such glass is subjected to less thermal stress and can withstand temperature differentials without fracturing of about 165 °C (300 °F). It is commonly used for the construction of reagent bottles and flasks, as well as lighting, electronics, and cookware.

<span class="mw-page-title-main">Laboratory flask</span> Type of laboratory glassware

Laboratory flasks are vessels or containers that fall into the category of laboratory equipment known as glassware. In laboratory and other scientific settings, they are usually referred to simply as flasks. Flasks come in a number of shapes and a wide range of sizes, but a common distinguishing aspect in their shapes is a wider vessel "body" and one narrower tubular sections at the top called necks which have an opening at the top. Laboratory flask sizes are specified by the volume they can hold, typically in metric units such as milliliters or liters. Laboratory flasks have traditionally been made of glass, but can also be made of plastic.

Glass tubes are mainly cylindrical hollow-wares. Their special shape combined with the huge variety of glass types, allows the use of glass tubing in many applications. For example, laboratory glassware, lighting applications, solar thermal systems and pharmaceutical packaging to name the largest.

A hermetic seal is any type of sealing that makes a given object airtight. The term originally applied to airtight glass containers, but as technology advanced it applied to a larger category of materials, including rubber and plastics. Hermetic seals are essential to the correct and safe functionality of many electronic and healthcare products. Used technically, it is stated in conjunction with a specific test method and conditions of use. Colloquially, the exact requirements of such a seal varies with the application.

<span class="mw-page-title-main">Soda–lime glass</span> Type of glass

Soda–lime glass, also called soda–lime–silica glass, is the most prevalent type of glass, used for windowpanes and glass containers for beverages, food, and some commodity items. Some glass bakeware is made of soda-lime glass, as opposed to the more common borosilicate glass. Soda–lime glass accounts for about 90% of manufactured glass.

<span class="mw-page-title-main">Beaker (laboratory equipment)</span> Glass container used in laboratories

In laboratory equipment, a beaker is generally a cylindrical container with a flat bottom. Most also have a small spout to aid pouring, as shown in the picture. Beakers are available in a wide range of sizes, from one milliliter up to several liters. A beaker is distinguished from a flask by having straight rather than sloping sides. The exception to this definition is a slightly conical-sided beaker called a Philips beaker. The beaker shape in general drinkware is similar.

<span class="mw-page-title-main">Wet chemistry</span> Form of analytical chemistry

Wet chemistry is a form of analytical chemistry that uses classical methods such as observation to analyze materials. The term wet chemistry is used as most analytical work is done in the liquid phase. Wet chemistry is also known as bench chemistry, since many tests are performed at lab benches.

<span class="mw-page-title-main">Round-bottom flask</span> Laboratory equipment

Round-bottom flasks are types of flasks having spherical bottoms used as laboratory glassware, mostly for chemical or biochemical work. They are typically made of glass for chemical inertness; and in modern days, they are usually made of heat-resistant borosilicate glass. There is at least one tubular section known as the neck with an opening at the tip. Two- or three-necked flasks are common as well. Round bottom flasks come in many sizes, from 5 mL to 20 L, with the sizes usually inscribed on the glass. In pilot plants even larger flasks are encountered.

<span class="mw-page-title-main">Schlenk line</span> Glass apparatus used in chemistry

The Schlenk line is a commonly used chemistry apparatus developed by Wilhelm Schlenk. It consists of a dual manifold with several ports. One manifold is connected to a source of purified inert gas, while the other is connected to a vacuum pump. The inert-gas line is vented through an oil bubbler, while solvent vapors and gaseous reaction products are prevented from contaminating the vacuum pump by a liquid-nitrogen or dry-ice/acetone cold trap. Special stopcocks or Teflon taps allow vacuum or inert gas to be selected without the need for placing the sample on a separate line.

<span class="mw-page-title-main">Schlenk flask</span> Reaction vessel used in air-sensitive chemistry

A Schlenk flask, or Schlenk tube, is a reaction vessel typically used in air-sensitive chemistry, invented by Wilhelm Schlenk. It has a side arm fitted with a PTFE or ground glass stopcock, which allows the vessel to be evacuated or filled with gases. These flasks are often connected to Schlenk lines, which allow both operations to be done easily.

<span class="mw-page-title-main">Ground glass joint</span> Used in laboratories to easily assemble apparatus from parts

Ground glass joints are used in laboratories to quickly and easily fit leak-tight apparatus together from interchangeable commonly available parts. For example, a round bottom flask, Liebig condenser, and oil bubbler with ground glass joints may be rapidly fitted together to reflux a reaction mixture. This is a large improvement compared with older methods of custom-made glassware, which was time-consuming and expensive, or the use of less chemical resistant and heat resistant corks or rubber bungs and glass tubes as joints, which took time to prepare as well.

<span class="mw-page-title-main">Eye dropper</span> Device used to transfer small quantities of liquids

An eye dropper, also called Pasteur pipette or simply dropper, is a device used to transfer small quantities of liquids. They are used in the laboratory and also to dispense small amounts of liquid medicines. A very common use was to dispense eye drops into the eye. The commonly recognized form is a glass tube tapered to a narrow point and fitted with a rubber bulb at the top, although many styles of both plastic and glass droppers exist. The combination of the pipette and rubber bulb has also been referred to as a teat pipette. The Pasteur pipette name is from the French scientist Louis Pasteur, who used a variant of them extensively during his research. In the past, there was no equipment to transfer a chemical solution without exposing it to the external environment. The hygiene and purity of chemical compounds is necessary for the expected result of each experiment. The eye dropper, both glass and plastic types, can be sterilized and plugged with a rubber bulb at the open end of the pipette preventing any contamination from the atmosphere. Generally, they are considered cheap enough to be disposable, however, so long as the glass point is not chipped, the eye dropper may be washed and reused indefinitely.

Air-free techniques refer to a range of manipulations in the chemistry laboratory for the handling of compounds that are air-sensitive. These techniques prevent the compounds from reacting with components of air, usually water and oxygen; less commonly carbon dioxide and nitrogen. A common theme among these techniques is the use of a fine (100–10−3 Torr) or high (10−3–10−6 Torr) vacuum to remove air, and the use of an inert gas: preferably argon, but often nitrogen.

A volumetric pipette, bulb pipette, or belly pipette allows extremely accurate measurement of the volume of a solution. It is calibrated to deliver accurately a fixed volume of liquid.

<span class="mw-page-title-main">Cannula transfer</span>

Cannula transfer or cannulation is a set of air-free techniques used with a Schlenk line, in transferring liquid or solution samples between reaction vessels via cannulae, avoiding atmospheric contamination. While the syringes are not the same as cannulae, the techniques remain relevant.

<span class="mw-page-title-main">Evaporating dish</span> A piece of laboratory glassware

An evaporating dish is a piece of laboratory glassware used for the evaporation of solutions and supernatant liquids, and sometimes to their melting point. Evaporating dishes are used to evaporate excess solvents – most commonly water – to produce a concentrated solution or a solid precipitate of the dissolved substance.

References

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