Limulus clotting enzyme

Limulus clotting enzyme
Identifiers
EC no.	3.4.21.86
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Limulus clotting enzyme (EC 3.4.21.86, clotting enzyme) is an enzyme. ^{[1] [2]} This enzyme catalyses the following chemical reaction:

Selective cleavage of -Arg¹⁸- and -Arg⁴⁷- bonds in coagulogen to form coagulin and fragments

This enzyme is present in the hemocyte granules of horseshoe crabs Limulus and Tachypleus . In the immunity-related clotting pathways of these organisms, it is the final enzyme responsible for the activation of coagulin. ^[3]

References

1. Muta T, Hashimoto R, Miyata T, Nishimura H, Toh Y, Iwanaga S (December 1990). "Proclotting enzyme from horseshoe crab hemocytes. cDNA cloning, disulfide locations, and subcellular localization". The Journal of Biological Chemistry. 265 (36): 22426–33. doi: 10.1016/S0021-9258(18)45722-5 . PMID   2266134.
2. Tokunaga F, Nakajima H, Iwanaga S (January 1991). "Further studies on lipopolysaccharide-sensitive serine protease zymogen (factor C): its isolation from Limulus polyphemus hemocytes and identification as an intracellular zymogen activated by alpha-chymotrypsin, not by trypsin". Journal of Biochemistry. 109 (1): 150–7. doi:10.1093/oxfordjournals.jbchem.a123337. PMID   2016264.
3. Iwanaga, S (May 2007). "Biochemical principle of Limulus test for detecting bacterial endotoxins". Proceedings of the Japan Academy. Series B, Physical and Biological Sciences. 83 (4): 110–9. Bibcode:2007PJAB...83..110I. doi:10.2183/pjab.83.110. PMC   3756735 . PMID   24019589.

External links

• Limulus+clotting+enzyme at the U.S. National Library of Medicine Medical Subject Headings (MeSH)