Microspore

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Microscopic photo of spores (in red) of Selaginella. The large three spores at the top are megaspores whereas the numerous smaller red spores at the bottom are microspores. Selaginella heterospores.jpeg
Microscopic photo of spores (in red) of Selaginella . The large three spores at the top are megaspores whereas the numerous smaller red spores at the bottom are microspores.

Microspores are land plant spores that develop into male gametophytes, whereas megaspores develop into female gametophytes. [1] The male gametophyte gives rise to sperm cells, which are used for fertilization of an egg cell to form a zygote. Megaspores are structures that are part of the alternation of generations in many seedless vascular cryptogams, all gymnosperms and all angiosperms. Plants with heterosporous life cycles using microspores and megaspores arose independently in several plant groups during the Devonian period. [2] Microspores are haploid, and are produced from diploid microsporocytes by meiosis. [3]

Contents

Morphology

The microspore has three different types of wall layers. The outer layer is called the perispore, the next is the exospore, and the inner layer is the endospore. The perispore is the thickest of the three layers while the exospore and endospore are relatively equal in width. [4]

Seedless vascular plants

In heterosporous seedless vascular plants, modified leaves called microsporophylls bear microsporangia containing many microsporocytes that undergo meiosis, each producing four microspores. Each microspore may develop into a male gametophyte consisting of a somewhat spherical antheridium within the microspore wall. Either 128 or 256 sperm cells with flagella are produced in each antheridium. [3] The only heterosporous ferns are aquatic or semi-aquatic, including the genera Marsilea , Regnellidium , Pilularia , Salvinia , and Azolla . Heterospory also occurs in the lycopods in the spikemoss genus Selaginella and in the quillwort genus Isoëtes .

Types of seedless vascular plants:

Gymnosperms

In seed plants the microspores develop into pollen grains each containing a reduced, multicellular male gametophyte. [5] The megaspores, in turn, develop into reduced female gametophytes that produce egg cells that, once fertilized, develop into seeds. Pollen cones or microstrobili usually develop toward the tips of the lower branches in clusters up to 50 or more. The microsporangia of gymnosperms develop in pairs toward the bases of the scales, which are therefore called microsporophylls. Each of the microsporocytes in the microsporangia undergoes meiosis, producing four haploid microspores. These develop into pollen grains, each consisting of four cells and, in conifers, a pair of external air sacs. The air sacs give the pollen grains added buoyancy that helps with wind dispersal. [3]

Types of Gymnosperms:

Angiosperms

As the anther of a flowering plant develops, four patches of tissue differentiate from the main mass of cells. These patches of tissue contain many diploid microsporocyte cells, each of which undergoes meiosis producing a quartet of microspores. Four chambers (pollen sacs) lined with nutritive tapetal cells are visible by the time the microspores are produced. After meiosis, the haploid microspores undergo several changes:

  1. The microspore divides by mitosis producing two cells. The first of the cells (the generative cell) is small and is formed inside the second larger cell (the tube cell).
  2. The members of each part of the microspores separate from each other.
  3. A double-layered wall then develops around each microspore.

These steps occur in sequence and when complete, the microspores have become pollen grains. [3]

Embryogenesis

Although it is not the usual route of a microspore, this process is the most effective way of yielding haploid and double haploid plants through the use of male sex hormones. [6] Under certain stressors such as heat or starvation, plants select for microspore embryogenesis. It was found that over 250 different species of angiosperms responded this way. [6] In the anther, after a microspore undergoes microsporogenesis, it can deviate towards embryogenesis and become star-like microspores. The microspore can then go one of four ways: Become an embryogenic microspore, undergo callogenesis to organogenesis (haploid/double haploid plant), become a pollen-like structure or die. [6]

Microspore embryogenesis is used in biotechnology to produce double haploid plants, which are immediately fixed as homozygous for each locus in only one generation. The haploid microspore is stressed to trigger the embryogenesis pathway and the resulting haploid embryo either doubles its genome spontaneously or with the help of chromosome doubling agents. Without this double haploid technology, conventional breeding methods would take several generations of selection to produce a homozygous line. [7]

See also

Related Research Articles

<span class="mw-page-title-main">Gametophyte</span> Haploid stage in the life cycle of plants and algae

A gametophyte is one of the two alternating multicellular phases in the life cycles of plants and algae. It is a haploid multicellular organism that develops from a haploid spore that has one set of chromosomes. The gametophyte is the sexual phase in the life cycle of plants and algae. It develops sex organs that produce gametes, haploid sex cells that participate in fertilization to form a diploid zygote which has a double set of chromosomes. Cell division of the zygote results in a new diploid multicellular organism, the second stage in the life cycle known as the sporophyte. The sporophyte can produce haploid spores by meiosis that on germination produce a new generation of gametophytes.

<span class="mw-page-title-main">Spore</span> Unit of reproduction adapted for dispersal and survival in unfavorable conditions

In biology, a spore is a unit of sexual or asexual reproduction that may be adapted for dispersal and for survival, often for extended periods of time, in unfavourable conditions. Spores form part of the life cycles of many plants, algae, fungi and protozoa.

<span class="mw-page-title-main">Sporangium</span> Enclosure in which spores are formed

A sporangium, also known as a "sporange", is an enclosure in which spores are formed. It can be composed of a single cell or can be multicellular. Virtually all plants, fungi, and many other lineages form sporangia at some point in their life cycle. Sporangia can produce spores by mitosis, but in nearly all land plants and many fungi, sporangia are the site of meiosis and produce genetically distinct haploid spores.

<span class="mw-page-title-main">Alternation of generations</span> Reproductive cycle of plants and algae

Alternation of generations is the predominant type of life cycle in plants and algae. In plants both phases are multicellular: the haploid sexual phase – the gametophyte – alternates with a diploid asexual phase – the sporophyte.

<span class="mw-page-title-main">Apomixis</span> Replacement of the normal sexual reproduction by asexual reproduction, without fertilization

In botany, apomixis is asexual development of seed or embryo without without fertilization. However, other definitions include replacement of the seed by a plantlet or replacement of the flower by bulbils.

<span class="mw-page-title-main">Gametogenesis</span> Biological process

Gametogenesis is a biological process by which diploid or haploid precursor cells undergo cell division and differentiation to form mature haploid gametes. Depending on the biological life cycle of the organism, gametogenesis occurs by meiotic division of diploid gametocytes into various gametes, or by mitosis. For example, plants produce gametes through mitosis in gametophytes. The gametophytes grow from haploid spores after sporic meiosis. The existence of a multicellular, haploid phase in the life cycle between meiosis and gametogenesis is also referred to as alternation of generations.

<span class="mw-page-title-main">Sporophyte</span> Diploid multicellular stage in the life cycle of a plant or alga

A sporophyte is the diploid multicellular stage in the life cycle of a plant or alga which produces asexual spores. This stage alternates with a multicellular haploid gametophyte phase.

<span class="mw-page-title-main">Ovule</span> Female plant reproductive structure

In seed plants, the ovule is the structure that gives rise to and contains the female reproductive cells. It consists of three parts: the integument, forming its outer layer, the nucellus, and the female gametophyte in its center. The female gametophyte — specifically termed a megagametophyte— is also called the embryo sac in angiosperms. The megagametophyte produces an egg cell for the purpose of fertilization. The ovule is a small structure present in the ovary. It is attached to the placenta by a stalk called a funicle. The funicle provides nourishment to the ovule.

<span class="mw-page-title-main">Antheridium</span> Part of a plant producing and containing male gametes

An antheridium is a haploid structure or organ producing and containing male gametes. The plural form is antheridia, and a structure containing one or more antheridia is called an androecium. Androecium is also the collective term for the stamens of flowering plants.

<span class="mw-page-title-main">Theca</span> Sheath or covering

In biology, a theca is a sheath or a covering.

Microsporangia are sporangia that produce microspores that give rise to male gametophytes when they germinate. Microsporangia occur in all vascular plants that have heterosporic life cycles, such as seed plants, spike mosses and the aquatic fern genus not species Azolla. In gymnosperms and angiosperm anthers, the microsporangia produce microsporocytes, the microspore mother cells, which then produce four microspores through the process of meiosis. Microsporocytes are produced in the microsporangia of gymnosperm cones and the anthers of angiosperms. They are diploid microspore mother-cells, which then produce four haploid microspores through the process of meiosis. These become pollen grains, within which the microspores divide twice by mitosis to produce a very simple gametophyte.

<span class="mw-page-title-main">Double fertilization</span> Complex fertilization mechanism of flowering plants

Double fertilization is a complex fertilization mechanism of flowering plants (angiosperms). This process involves the joining of a female gametophyte with two male gametes (sperm). It begins when a pollen grain adheres to the stigma of the carpel, the female reproductive structure of a flower. The pollen grain then takes in moisture and begins to germinate, forming a pollen tube that extends down toward the ovary through the style. The tip of the pollen tube then enters the ovary and penetrates through the micropyle opening in the ovule. The pollen tube proceeds to release the two sperm in the embryo sac.

A gametangium is an organ or cell in which gametes are produced that is found in many multicellular protists, algae, fungi, and the gametophytes of plants. In contrast to gametogenesis in animals, a gametangium is a haploid structure and formation of gametes does not involve meiosis.

<span class="mw-page-title-main">Megaspore</span> Large spore in heterosporous plants that germinates into a female gametophyte

Megaspores, also called macrospores, are a type of spore that is present in heterosporous plants. These plants have two spore types, megaspores and microspores. Generally speaking, the megaspore, or large spore, germinates into a female gametophyte, which produces egg cells. These are fertilized by sperm produced by the male gametophyte developing from the microspore. Heterosporous plants include seed plants, water ferns (Salviniales), spikemosses (Selaginellaceae) and quillworts (Isoetaceae).

Plant reproduction is the production of new offspring in plants, which can be accomplished by sexual or asexual reproduction. Sexual reproduction produces offspring by the fusion of gametes, resulting in offspring genetically different from either parent. Asexual reproduction produces new individuals without the fusion of gametes, resulting in clonal plants that are genetically identical to the parent plant and each other, unless mutations occur.

Microgametogenesis is the process in plant reproduction where a microgametophyte develops in a pollen grain to the three-celled stage of its development. In flowering plants it occurs with a microspore mother cell inside the anther of the plant.

Megagametogenesis is the process of maturation of the female gametophyte, or megagametophyte, in plants During the process of megagametogenesis, the megaspore, which arises from megasporogenesis, develops into the embryo sac, which is where the female gamete is housed. These megaspores then develop into the haploid female gametophytes. This occurs within the ovule, which is housed inside the ovary.

Sporogenesis is the production of spores in biology. The term is also used to refer to the process of reproduction via spores. Reproductive spores were found to be formed in eukaryotic organisms, such as plants, algae and fungi, during their normal reproductive life cycle. Dormant spores are formed, for example by certain fungi and algae, primarily in response to unfavorable growing conditions. Most eukaryotic spores are haploid and form through cell division, though some types are diploid sor dikaryons and form through cell fusion.we can also say this type of reproduction as single pollination

<span class="mw-page-title-main">Embryonic sac</span>

A megaspore mother cell, or megasporocyte, is a diploid cell in plants in which meiosis will occur, resulting in the production of four haploid megaspores. At least one of the spores develop into haploid female gametophytes (megagametophytes). The megaspore mother cell arises within the megasporangium tissue.

<span class="mw-page-title-main">Heterospory</span> The production of spores of two different sizes and sexes by several groups of land plants

Heterospory is the production of spores of two different sizes and sexes by the sporophytes of land plants. The smaller of these, the microspore, is male and the larger megaspore is female. Heterospory evolved during the Devonian period from isospory independently in several plant groups: the clubmosses, the ferns including the arborescent horsetails, and progymnosperms. This occurred as part of the process of evolution of the timing of sex differentiation.

References

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